1. Development of conductometric biosensor array for simultaneous determination of maltose, lactose, sucrose and glucose.
- Author
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Soldatkin OO, Peshkova VM, Saiapina OY, Kucherenko IS, Dudchenko OY, Melnyk VG, Vasylenko OD, Semenycheva LM, Soldatkin AP, and Dzyadevych SV
- Subjects
- Calibration, Conductometry instrumentation, Enzymes, Immobilized chemistry, Hydrogen-Ion Concentration, Osmolar Concentration, Reproducibility of Results, Sensitivity and Specificity, Solutions, Transducers, Water, Biosensing Techniques instrumentation, Conductometry methods, Glucose analysis, Lactose analysis, Maltose analysis, Sucrose analysis
- Abstract
The aim of this work was to develop an array of biosensors for simultaneous determination of four carbohydrates in solution. Several enzyme systems selective to lactose, maltose, sucrose and glucose were immobilised on the surface of four conductometric transducers and served as bio-recognition elements of the biosensor array. Direct enzyme analysis carried out by the developed biosensors was highly sensitive to the corresponding substrates. The analysis lasted 2 min. The dynamic range of substrate determination extended from 0.001 mM to 1.0-3.0mM, and strongly depended on the enzyme system used. An effect of the solution pH, ionic strength and buffer capacity on the biosensors responses was investigated; the conditions of simultaneous operation of all biosensors were optimised. The data on cross-impact of the substrates of all biosensors were obtained; the biosensor selectivity towards possible interfering carbohydrates was tested. The developed biosensor array showed good signal reproducibility and storage stability. The biosensor array is suited for simultaneous, quick, simple, and selective determination of maltose, lactose, sucrose and glucose., (© 2013 Elsevier B.V. All rights reserved.)
- Published
- 2013
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