25 results on '"Ducheix, Simon"'
Search Results
2. Lipidomic analysis of adipose-derived extracellular vesicles reveals specific EV lipid sorting informative of the obesity metabolic state.
- Author
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Blandin A, Dugail I, Hilairet G, Ponnaiah M, Ghesquière V, Froger J, Ducheix S, Fizanne L, Boursier J, Cariou B, Lhomme M, and Le Lay S
- Subjects
- Animals, Mice, Chromatography, Liquid, Tandem Mass Spectrometry, Obesity metabolism, Sphingomyelins metabolism, Lipidomics, Extracellular Vesicles metabolism
- Abstract
Adipose extracellular vesicles (AdEVs) transport lipids that could participate in the development of obesity-related metabolic dysfunctions. This study aims to define mouse AdEV lipid signature by a targeted LC-MS/MS approach in either healthy or obesity context. Distinct clustering of AdEV and visceral adipose tissue (VAT) lipidomes by principal component analysis reveals specific AdEV lipid sorting when compared with secreting VAT. Comprehensive analysis identifies enrichment of ceramides, sphingomyelins, and phosphatidylglycerols species in AdEVs compared with source VAT whose lipid content closely relates to the obesity status and is influenced by the diet. Obesity moreover impacts AdEV lipidome, mirroring lipid alterations retrieved in plasma and VAT. Overall, our study identifies specific lipid fingerprints for plasma, VAT, and AdEVs that are informative of the metabolic status. Lipid species enriched in AdEVs in the obesity context may constitute biomarker candidates or mediators of the obesity-associated metabolic dysfunctions., Competing Interests: Declaration of interests There are no conflicts of interest to declare., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2023
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3. Reduction in gut-derived MUFAs via intestinal stearoyl-CoA desaturase 1 deletion drives susceptibility to NAFLD and hepatocarcinoma.
- Author
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Ducheix S, Piccinin E, Peres C, Garcia-Irigoyen O, Bertrand-Michel J, Fouache A, Cariello M, Lobaccaro JM, Guillou H, Sabbà C, Ntambi JM, and Moschetta A
- Subjects
- Animals, Cholesterol, Diet, Western, Fatty Acids, Fatty Acids, Monounsaturated metabolism, Fibrosis, Inflammation, Mice, Stearoyl-CoA Desaturase genetics, Triglycerides metabolism, Carcinoma, Hepatocellular genetics, Liver Neoplasms genetics, Non-alcoholic Fatty Liver Disease genetics
- Abstract
Nonalcoholic fatty liver disease (NAFLD) is defined by a set of hepatic conditions ranging from steatosis to steatohepatitis (NASH), characterized by inflammation and fibrosis, eventually predisposing to hepatocellular carcinoma (HCC). Together with fatty acids (FAs) originated from adipose lipolysis and hepatic lipogenesis, intestinal-derived FAs are major contributors of steatosis. However, the role of mono-unsaturated FAs (MUFAs) in NAFLD development is still debated. We previously established the intestinal capacity to produce MUFAs, but its consequences in hepatic functions are still unknown. Here, we aimed to determine the role of the intestinal MUFA-synthetizing enzyme stearoyl-CoA desaturase 1 (SCD1) in NAFLD. We used intestinal-specific Scd1-KO (iScd1
-/- ) mice and studied hepatic dysfunction in different models of steatosis, NASH, and HCC. Intestinal-specific Scd1 deletion decreased hepatic MUFA proportion. Compared with controls, iScd1-/- mice displayed increased hepatic triglyceride accumulation and derangement in cholesterol homeostasis when fed a MUFA-deprived diet. Then, on Western diet feeding, iScd1-/- mice triggered inflammation and fibrosis compared with their wild-type littermates. Finally, intestinal-Scd1 deletion predisposed mice to liver cancer. Conclusions: Collectively, these results highlight the major importance of intestinal MUFA metabolism in maintaining hepatic functions and show that gut-derived MUFAs are protective from NASH and HCC., (© 2022 The Authors. Hepatology Communications published by Wiley Periodicals LLC on behalf of American Association for the Study of Liver Diseases.)- Published
- 2022
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4. Seipin localizes at endoplasmic-reticulum-mitochondria contact sites to control mitochondrial calcium import and metabolism in adipocytes.
- Author
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Combot Y, Salo VT, Chadeuf G, Hölttä M, Ven K, Pulli I, Ducheix S, Pecqueur C, Renoult O, Lak B, Li S, Karhinen L, Belevich I, Le May C, Rieusset J, Le Lay S, Croyal M, Tayeb KS, Vihinen H, Jokitalo E, Törnquist K, Vigouroux C, Cariou B, Magré J, Larhlimi A, Ikonen E, and Prieur X
- Subjects
- Adipose Tissue metabolism, Animals, Calcium metabolism, Cell Line, Endoplasmic Reticulum metabolism, Endoplasmic Reticulum Stress, Energy Metabolism physiology, GTP-Binding Protein gamma Subunits deficiency, GTP-Binding Protein gamma Subunits physiology, Humans, Lipid Droplets metabolism, Lipid Metabolism physiology, Lipids physiology, Male, Mice, Mice, Inbred C57BL, Adipocytes metabolism, GTP-Binding Protein gamma Subunits metabolism, Mitochondria metabolism
- Abstract
Deficiency of the endoplasmic reticulum (ER) protein seipin results in generalized lipodystrophy by incompletely understood mechanisms. Here, we report mitochondrial abnormalities in seipin-deficient patient cells. A subset of seipin is enriched at ER-mitochondria contact sites (MAMs) in human and mouse cells and localizes in the vicinity of calcium regulators SERCA2, IP3R, and VDAC. Seipin association with MAM calcium regulators is stimulated by fasting-like stimuli, while seipin association with lipid droplets is promoted by lipid loading. Acute seipin removal does not alter ER calcium stores but leads to defective mitochondrial calcium import accompanied by a widespread reduction in Krebs cycle metabolites and ATP levels. In mice, inducible seipin deletion leads to mitochondrial dysfunctions preceding the development of metabolic complications. Together, these data suggest that seipin controls mitochondrial energy metabolism by regulating mitochondrial calcium influx at MAMs. In seipin-deficient adipose tissue, reduced ATP production compromises adipocyte properties, contributing to lipodystrophy pathogenesis., Competing Interests: Declaration of interests The authors declare no competing interests., (Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.)
- Published
- 2022
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5. Proton NMR Enables the Absolute Quantification of Aqueous Metabolites and Lipid Classes in Unique Mouse Liver Samples.
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Amiel A, Tremblay-Franco M, Gautier R, Ducheix S, Montagner A, Polizzi A, Debrauwer L, Guillou H, Bertrand-Michel J, and Canlet C
- Abstract
Hepatic metabolites provide valuable information on the physiological state of an organism, and thus, they are monitored in many clinical situations. Typically, monitoring requires several analyses for each class of targeted metabolite, which is time consuming. The present study aimed to evaluate a proton nuclear magnetic resonance (
1 H-NMR) method for obtaining quantitative measurements of aqueous and lipidic metabolites. We optimized the extraction protocol, the standard samples, and the organic solvents for the absolute quantification of lipid species. To validate the method, we analyzed metabolic profiles in livers of mice fed three different diets. We compared our results with values obtained with conventional methods and found strong correlations. The1 H-NMR protocol enabled the absolute quantification of 29 aqueous metabolites and eight lipid classes. Results showed that mice fed a diet enriched in saturated fatty acids had higher levels of triglycerides, cholesterol ester, monounsaturated fatty acids, lactate, 3-hydroxy-butyrate, and alanine and lower levels of glucose, compared to mice fed a control diet. In conclusion, proton NMR provided a rapid overview of the main lipid classes (triglycerides, cholesterol, phospholipids, fatty acids) and the most abundant aqueous metabolites in liver.- Published
- 2019
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6. PGC-1β Induces Susceptibility To Acetaminophen-Driven Acute Liver Failure.
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Piccinin E, Ducheix S, Peres C, Arconzo M, Vegliante MC, Ferretta A, Bellafante E, Villani G, and Moschetta A
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- Animals, Disease Models, Animal, Liver metabolism, Liver Failure, Acute chemically induced, Liver Failure, Acute genetics, Male, Mice, Mice, Inbred C57BL, Oxidative Stress, Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha metabolism, Acetaminophen adverse effects, Diet, High-Fat adverse effects, Liver Failure, Acute metabolism, Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha genetics
- Abstract
Acetaminophen (APAP) is a worldwide commonly used painkiller drug. However, high doses of APAP can lead to acute hepatic failure and, in some cases, death. Previous studies indicated that different factors, including life-style and metabolic diseases, could predispose to the risk of APAP-induced liver failure. However, the molecular process that could favor APAP hepatotoxicity remains understood. Here, we reported that a short-term high fat-enriched diet worsens APAP-induced liver damage, by promoting liver accumulation of lipids that induces the activation of peroxisome proliferator-activated receptor gamma coactivator 1-beta (PGC-1β). Therefore, we challenged mice with hepatic-specific PGC-1β overexpression on a chow diet with a subtoxic dose of APAP and we found that PGC-1β overexpression renders the liver more sensitive to APAP damage, mainly due to intense oxidative stress, finally ending up with liver necrosis and mice death. Overall, our results indicated that during high fat feeding, PGC-1β adversely influences the ability of the liver to overcome APAP toxicity by orchestrating different metabolic pathways that finally lead to fatal outcome.
- Published
- 2019
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7. Role of Oleic Acid in the Gut-Liver Axis: From Diet to the Regulation of Its Synthesis via Stearoyl-CoA Desaturase 1 (SCD1).
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Piccinin E, Cariello M, De Santis S, Ducheix S, Sabbà C, Ntambi JM, and Moschetta A
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- Diet, Gastrointestinal Tract physiology, Humans, Liver physiology, Oleic Acid biosynthesis, Gastrointestinal Tract drug effects, Liver drug effects, Oleic Acid metabolism, Stearoyl-CoA Desaturase metabolism
- Abstract
The consumption of an olive oil rich diet has been associated with the diminished incidence of cardiovascular disease and cancer. Several studies have attributed these beneficial effects to oleic acid (C18 n-9), the predominant fatty acid principal component of olive oil. Oleic acid is not an essential fatty acid since it can be endogenously synthesized in humans. Stearoyl-CoA desaturase 1 (SCD1) is the enzyme responsible for oleic acid production and, more generally, for the synthesis of monounsaturated fatty acids (MUFA). The saturated to monounsaturated fatty acid ratio affects the regulation of cell growth and differentiation, and alteration in this ratio has been implicated in a variety of diseases, such as liver dysfunction and intestinal inflammation. In this review, we discuss our current understanding of the impact of gene-nutrient interactions in liver and gut diseases, by taking advantage of the role of SCD1 and its product oleic acid in the modulation of different hepatic and intestinal metabolic pathways.
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- 2019
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8. The protective role of liver X receptor (LXR) during fumonisin B1-induced hepatotoxicity.
- Author
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Régnier M, Polizzi A, Lukowicz C, Smati S, Lasserre F, Lippi Y, Naylies C, Laffitte J, Bétoulières C, Montagner A, Ducheix S, Gourbeyre P, Ellero-Simatos S, Menard S, Bertrand-Michel J, Al Saati T, Lobaccaro JM, Burger HM, Gelderblom WC, Guillou H, Oswald IP, and Loiseau N
- Subjects
- Alanine Transaminase metabolism, Animals, Aspartate Aminotransferases metabolism, Chemical and Drug Induced Liver Injury etiology, Chemical and Drug Induced Liver Injury pathology, Female, Fumonisins blood, Gene Expression Regulation drug effects, Liver drug effects, Liver physiology, Liver X Receptors genetics, Mice, Inbred C57BL, Mice, Knockout, Sphingolipids metabolism, Chemical and Drug Induced Liver Injury metabolism, Fumonisins toxicity, Liver X Receptors metabolism
- Abstract
Fumonisin B1 (FB1), a congener of fumonisins produced by Fusarium species, is the most abundant and most toxicologically active fumonisin. FB1 causes severe mycotoxicosis in animals, including nephrotoxicity, hepatotoxicity, and disruption of the intestinal barrier. However, mechanisms associated with FB1 toxicity are still unclear. Preliminary studies have highlighted the role of liver X receptors (LXRs) during FB1 exposure. LXRs belong to the nuclear receptor family and control the expression of genes involved in cholesterol and lipid homeostasis. In this context, the toxicity of FB1 was compared in female wild-type (LXR
+/+ ) and LXRα,β double knockout (LXR-/- ) mice in the absence or presence of FB1 (10 mg/kg body weight/day) for 28 days. Exposure to FB1 supplemented in the mice's drinking water resulted in more pronounced hepatotoxicity in LXR-/- mice compared to LXR+/+ mice, as indicated by hepatic transaminase levels (ALT, AST) and hepatic inflammatory and fibrotic lesions. Next, the effect of FB1 exposure on the liver transcriptome was investigated. FB1 exposure led to a specific transcriptional response in LXR-/- mice that included altered cholesterol and bile acid homeostasis. ELISA showed that these effects were associated with an elevated FB1 concentration in the plasma of LXR-/- mice, suggesting that LXRs participate in intestinal absorption and/or clearance of the toxin. In summary, this study demonstrates an important role of LXRs in protecting the liver against FB1-induced toxicity, suggesting an alternative mechanism not related to the inhibition of sphingolipid synthesis for mycotoxin toxicity.- Published
- 2019
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9. Deletion of Stearoyl-CoA Desaturase-1 From the Intestinal Epithelium Promotes Inflammation and Tumorigenesis, Reversed by Dietary Oleate.
- Author
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Ducheix S, Peres C, Härdfeldt J, Frau C, Mocciaro G, Piccinin E, Lobaccaro JM, De Santis S, Chieppa M, Bertrand-Michel J, Plateroti M, Griffin JL, Sabbà C, Ntambi JM, and Moschetta A
- Subjects
- Animals, Female, Intestinal Mucosa pathology, Male, Mice, Mice, Inbred C57BL, Oleic Acid metabolism, Tumor Burden, Dietary Fats, Unsaturated administration & dosage, Enteritis etiology, Intestinal Mucosa enzymology, Intestinal Neoplasms etiology, Oleic Acid administration & dosage, Stearoyl-CoA Desaturase physiology
- Abstract
Background & Aims: The enzyme stearoyl-coenzyme A desaturase 1 (SCD or SCD1) produces monounsaturated fatty acids by introducing double bonds into saturated bonds between carbons 9 and 10, with oleic acid as the main product. SCD1 is present in the intestinal epithelium, and fatty acids regulate cell proliferation, so we investigated the effects of SCD1-induced production of oleic acid in enterocytes in mice., Methods: We generated mice with disruption of Scd1 selectively in the intestinal epithelium (iScd1
-/- mice) on a C57BL/6 background; iScd1+/+ mice were used as controls. We also generated iScd1-/- ApcMin/+ mice and studied cancer susceptibility. Mice were fed a chow, oleic acid-deficient, or oleic acid-rich diet. Intestinal tissues were collected and analyzed by histology, reverse transcription quantitative polymerase chain reaction, immunohistochemistry, and mass spectrometry, and tumors were quantified and measured., Results: Compared with control mice, the ileal mucosa of iScd1-/- mice had a lower proportion of palmitoleic (C16:1 n-7) and oleic acids (C18:1 n-9), with accumulation of stearic acid (C18:0); this resulted a reduction of the Δ9 desaturation ratio between monounsaturated (C16:1 n-7 and C18:1 n-9) and saturated (C16:0 and C18:0) fatty acids. Ileal tissues from iScd1-/- mice had increased expression of markers of inflammation activation and crypt proliferative genes compared with control mice. The iScd1-/- ApcMin/+ mice developed more and larger tumors than iScd1+/+ ApcMin/+ mice. iScd1-/- ApcMin/+ mice fed the oleic acid-rich diet had reduced intestinal inflammation and significantly lower tumor burden compared with mice fed a chow diet., Conclusions: In studies of mice, we found intestinal SCD1 to be required for synthesis of oleate in the enterocytes and maintenance of fatty acid homeostasis. Dietary supplementation with oleic acid reduces intestinal inflammation and tumor development in mice., (Copyright © 2018 AGA Institute. Published by Elsevier Inc. All rights reserved.)- Published
- 2018
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10. Chronic O-GlcNAcylation and Diabetic Cardiomyopathy: The Bitterness of Glucose.
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Ducheix S, Magré J, Cariou B, and Prieur X
- Abstract
Type 2 diabetes (T2D) is a major risk factor for heart failure. Diabetic cardiomyopathy (DC) is characterized by diastolic dysfunction and left ventricular hypertrophy. Epidemiological data suggest that hyperglycaemia contributes to the development of DC. Several cellular pathways have been implicated in the deleterious effects of high glucose concentrations in the heart: oxidative stress, accumulation of advanced glycation end products (AGE), and chronic hexosamine biosynthetic pathway (HBP) activation. In the present review, we focus on the effect of chronic activation of the HBP on diabetic heart function. The HBP supplies N-acetylglucosamine moiety (O-GlcNAc) that is O-linked by O-GlcNAc transferase (OGT) to proteins on serine or threonine residues. This post-translational protein modification modulates the activity of the targeted proteins. In the heart, acute activation of the HBP in response to ischaemia-reperfusion injury appears to be protective. Conversely, chronic activation of the HBP in the diabetic heart affects Ca
2+ handling, contractile properties, and mitochondrial function and promotes stress signaling, such as left ventricular hypertrophy and endoplasmic reticulum stress. Many studies have shown that O-GlcNAc impairs the function of key protein targets involved in these pathways, such as phospholamban, calmodulin kinase II, troponin I, and FOXO1. The data show that excessive O-GlcNAcylation is a major trigger of the glucotoxic events that affect heart function under chronic hyperglycaemia. Supporting this finding, pharmacological or genetic inhibition of the HBP in the diabetic heart improves heart function. In addition, the SGLT2 inhibitor dapagliflozin, a glucose lowering agent, has recently been shown to lower cardiac HBP in a lipodystophic T2D mice model and to concomitantly improve the diastolic dysfunction of these mice. Therefore, targeting cardiac-excessive O-GlcNAcylation or specific target proteins represents a potential therapeutic option to treat glucotoxicity in the diabetic heart.- Published
- 2018
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11. LXRs, SHP, and FXR in Prostate Cancer: Enemies or Ménage à Quatre With AR?
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Cariello M, Ducheix S, Maqdasy S, Baron S, Moschetta A, and Lobaccaro JA
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- Animals, Humans, Male, Prostatic Neoplasms physiopathology, Liver X Receptors metabolism, Prostatic Neoplasms metabolism, Receptors, Androgen metabolism, Receptors, Cytoplasmic and Nuclear metabolism
- Abstract
Androgens and androgen receptor (AR, NR3C4) clearly play a crucial role in prostate cancer progression. Besides, the link between metabolic disorders and the risk of developing a prostate cancer has been emerging these last years. Interestingly, "lipid" nuclear receptors such as LXRα/NR1H3 and LXRβ/NR1H2 (as well as FXRα/NR1H4 and SHP/NR0B2) have been described to decrease the lipid metabolism, while AR increases it. Moreover, these former orphan nuclear receptors can regulate androgen levels and modulate AR activity. Thus, it is not surprising to find such receptors involved in the physiology of prostate. This review is focused on the roles of liver X receptors (LXRs), farnesoid X receptor (FXR), and small heterodimeric partner (SHP) in prostate physiology and their capabilities to interfere with the androgen-regulated pathways by modulating the levels of active androgen within the prostate. By the use of prostate cancer cell lines, mice deficient for these nuclear receptors and human tissue libraries, several authors have pointed out the putative possibility to pharmacologically target these receptors. These data open a new field of research for the development of new drugs that could overcome the castration resistance in prostate cancer, a usual phenomenon in patients., Competing Interests: Declaration of Conflicting Interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
- Published
- 2018
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12. Dual extraction of mRNA and lipids from a single biological sample.
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Podechard N, Ducheix S, Polizzi A, Lasserre F, Montagner A, Legagneux V, Fouché E, Saez F, Lobaccaro JM, Lakhal L, Ellero-Simatos S, Martin PG, Loiseau N, Bertrand-Michel J, and Guillou H
- Subjects
- Animals, Chemical Fractionation methods, Gene Expression Profiling, Lipids chemistry, Liver chemistry, Liver metabolism, Mice, RNA, Messenger chemistry, Reproducibility of Results, Lipids isolation & purification, RNA, Messenger isolation & purification
- Abstract
The extraction of RNA and lipids from a large number of biological samples is time-consuming and costly with steps required for both transcriptomic and lipidomic approaches. Most protocols rely on independent extraction of nucleic acids and lipids from a single sample, thereby increasing the need for biological material and inducing variability in data analysis. We investigated whether it is possible to use a standard RNA extraction procedure to analyze not only RNA levels, but also lipids in a single liver sample. We show that the organic phase obtained when using standard reagents for RNA extraction can be used to analyze lipids, including neutral lipids and fatty acids, by gas chromatography. We applied this technique to an analysis of lipids and the associated gene expression pattern in mice with hepatic steatosis induced by pharmacological activation of nuclear receptor LXR.
- Published
- 2018
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13. Hepatic peroxisome proliferator-activated receptor γ coactivator 1β drives mitochondrial and anabolic signatures that contribute to hepatocellular carcinoma progression in mice.
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Piccinin E, Peres C, Bellafante E, Ducheix S, Pinto C, Villani G, and Moschetta A
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- Animals, Blotting, Western, Carcinoma, Hepatocellular pathology, Disease Progression, Gene Expression Regulation, Neoplastic, Immunohistochemistry, Lipid Metabolism genetics, Liver pathology, Liver Neoplasms pathology, Metabolism genetics, Mice, Mice, Knockout, Mitochondria metabolism, Reactive Oxygen Species metabolism, Real-Time Polymerase Chain Reaction, Carcinogenesis metabolism, Carcinoma, Hepatocellular metabolism, Liver Neoplasms metabolism, Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha metabolism
- Abstract
The peroxisome proliferator-activated receptor γ (PPARγ) coactivator-1β (PGC-1 β) is a master regulator of mitochondrial biogenesis and oxidative metabolism as well as of antioxidant defense. Specifically, in the liver, PGC-1β also promotes de novo lipogenesis, thus sustaining cellular anabolic processes. Given the relevant pathogenic role of mitochondrial and fatty acid metabolism in hepatocarcinoma (HCC), here we pointed to PGC-1β as a putative novel transcriptional player in the development and progression of HCC. For this purpose, we generated both hepatic-specific PGC-1β-overexpressing (LivPGC-1β) and PGC-1β knockout (LivPGC-1βKO) mice, and we challenged them with both chemical and genetic models of hepatic carcinogenesis. Our results demonstrate a pivotal role of PGC-1β in driving liver tumor development. Indeed, whereas mice overexpressing PGC-1β show greater tumor susceptibility, PGC-1β knockout mice are protected from carcinogenesis. High levels of PGC-1β are able to boost reactive oxygen species (ROS) scavenger expression, therefore limiting the detrimental ROS accumulation and, consequently, apoptosis. Moreover, it supports tumor anabolism, enhancing the expression of genes involved in fatty acid and triglyceride synthesis. Accordingly, the specific hepatic ablation of PGC-1β promotes the accumulation of ROS-driven macromolecule damage, finally limiting tumor growth., Conclusion: The present data elect hepatic PGC-1β as a transcriptional gatekeeper of mitochondrial function and redox status in HCC, orchestrating different metabolic programs that allow tumor progression. (Hepatology 2018;67:884-898)., (© 2017 by the American Association for the Study of Liver Diseases.)
- Published
- 2018
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14. Dietary oleic acid regulates hepatic lipogenesis through a liver X receptor-dependent signaling.
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Ducheix S, Montagner A, Polizzi A, Lasserre F, Régnier M, Marmugi A, Benhamed F, Bertrand-Michel J, Mselli-Lakhal L, Loiseau N, Martin PG, Lobaccaro JM, Ferrier L, Postic C, and Guillou H
- Subjects
- Animal Feed, Animals, Diet, Gene Expression Profiling, Immunoblotting, Inflammation metabolism, Inflammation pathology, Liver pathology, Liver Diseases metabolism, Liver Diseases pathology, Liver X Receptors genetics, Male, Mice, Inbred C57BL, Mice, Transgenic, Models, Animal, Protein Isoforms, Dietary Fats metabolism, Lipogenesis physiology, Liver metabolism, Liver X Receptors metabolism, Oleic Acid metabolism, Olive Oil metabolism
- Abstract
Olive oil consumption is beneficial for health as it is associated with a decreased prevalence of cancer and cardiovascular diseases. Oleic acid is, by far, the most abundant component of olive oil. Since it can be made through de novo synthesis in animals, it is not an essential fatty acid. While it has become clear that dietary oleic acid regulates many biological processes, the signaling pathway involved in these regulations remains poorly defined. In this work we tested the impact of an oleic acid-rich diet on hepatic gene expression. We were particularly interested in addressing the contribution of Liver X Receptors (LXR) in the control of genes involved in hepatic lipogenesis, an essential process in whole body energy homeostasis. We used wild-type mice and transgenic mice deficient for both α and β Liver X Receptor isoforms (LXR-/-) fed a control or an oleate enriched diet. We observed that hepatic-lipid accumulation was enhanced as well as the expression of lipogenic genes in the liver of wild-type mice fed the oleate enriched diet. In contrast, none of these changes occurred in the liver of LXR-/- mice. Strikingly, oleate-rich diet reduced cholesterolemia in wild-type mice and induced signs of liver inflammation and damage in LXR-/- mice but not in wild-type mice. This work suggests that dietary oleic acid reduces cholesterolemia while promoting LXR-dependent hepatic lipogenesis without detrimental effects to the liver.
- Published
- 2017
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15. Is hepatic lipogenesis fundamental for NAFLD/NASH? A focus on the nuclear receptor coactivator PGC-1β.
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Ducheix S, Vegliante MC, Villani G, Napoli N, Sabbà C, and Moschetta A
- Subjects
- Animals, Disease Progression, Humans, Models, Biological, Non-alcoholic Fatty Liver Disease epidemiology, Non-alcoholic Fatty Liver Disease pathology, Lipogenesis, Liver metabolism, Non-alcoholic Fatty Liver Disease metabolism, Nuclear Receptor Coactivators metabolism
- Abstract
Non-alcoholic fatty liver diseases are the hepatic manifestation of metabolic syndrome. According to the classical pattern of NAFLD progression, de novo fatty acid synthesis has been incriminated in NAFLD progression. However, this hypothesis has been challenged by the re-evaluation of NAFLD development mechanisms together with the description of the role of lipogenic genes in NAFLD and with the recent observation that PGC-1β, a nuclear receptor/transcription factor coactivator involved in the transcriptional regulation of lipogenesis, displays protective effects against NAFLD/NASH progression. In this review, we focus on the implication of lipogenesis and triglycerides synthesis on the development of non-alcoholic fatty liver diseases and discuss the involvement of these pathways in the protective role of PGC-1β toward these hepatic manifestations.
- Published
- 2016
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16. Hepatic Fasting-Induced PPARα Activity Does Not Depend on Essential Fatty Acids.
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Polizzi A, Fouché E, Ducheix S, Lasserre F, Marmugi AP, Mselli-Lakhal L, Loiseau N, Wahli W, Guillou H, and Montagner A
- Subjects
- Alanine Transaminase blood, Animals, Aspartate Aminotransferases blood, Body Weight, Cholesterol blood, Cytochrome P-450 Enzyme System genetics, Cytochrome P450 Family 4 genetics, Fasting, Fatty Liver metabolism, Fatty Liver pathology, Fibroblast Growth Factors genetics, Liver pathology, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, PPAR alpha metabolism, RNA, Messenger metabolism, Triglycerides blood, Dietary Fats, Liver metabolism, PPAR alpha genetics
- Abstract
The liver plays a central role in the regulation of fatty acid metabolism, which is highly sensitive to transcriptional responses to nutrients and hormones. Transcription factors involved in this process include nuclear hormone receptors. One such receptor, PPARα, which is highly expressed in the liver and activated by a variety of fatty acids, is a critical regulator of hepatic fatty acid catabolism during fasting. The present study compared the influence of dietary fatty acids and fasting on hepatic PPARα-dependent responses. Pparα(-/-) male mice and their wild-type controls were fed diets containing different fatty acids for 10 weeks prior to being subjected to fasting or normal feeding. In line with the role of PPARα in sensing dietary fatty acids, changes in chronic dietary fat consumption influenced liver damage during fasting. The changes were particularly marked in mice fed diets lacking essential fatty acids. However, fasting, rather than specific dietary fatty acids, induced acute PPARα activity in the liver. Taken together, the data imply that the potent signalling involved in triggering PPARα activity during fasting does not rely on essential fatty acid-derived ligand., Competing Interests: The authors declare no conflicts of interest. The founding sponsors had no role in the design of the study; the collection, analysis, or interpretation of data; writing the manuscript; or the decision to publish the results.
- Published
- 2016
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17. Activation of the Constitutive Androstane Receptor induces hepatic lipogenesis and regulates Pnpla3 gene expression in a LXR-independent way.
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Marmugi A, Lukowicz C, Lasserre F, Montagner A, Polizzi A, Ducheix S, Goron A, Gamet-Payrastre L, Gerbal-Chaloin S, Pascussi JM, Moldes M, Pineau T, Guillou H, and Mselli-Lakhal L
- Subjects
- Animals, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors, Cell Line, Cells, Cultured, Constitutive Androstane Receptor, Female, Gene Expression Regulation drug effects, Hep G2 Cells, Hepatocytes drug effects, Hepatocytes metabolism, Humans, Lipase genetics, Lipase metabolism, Liver drug effects, Liver X Receptors genetics, Liver X Receptors metabolism, Male, Membrane Proteins genetics, Membrane Proteins metabolism, Mice, Inbred C57BL, Mice, Knockout, Nuclear Proteins genetics, Nuclear Proteins metabolism, Phenobarbital pharmacology, Pyridines pharmacology, RNA, Messenger metabolism, Transcription Factors genetics, Transcription Factors metabolism, Fatty Liver metabolism, Lipogenesis drug effects, Liver metabolism, Receptors, Cytoplasmic and Nuclear agonists, Receptors, Cytoplasmic and Nuclear genetics
- Abstract
The Constitutive Androstane Receptor (CAR, NR1I3) has been newly described as a regulator of energy metabolism. A relevant number of studies using animal models of obesity suggest that CAR activation could be beneficial on the metabolic balance. However, this remains controversial and the underlying mechanisms are still unknown. This work aimed to investigate the effect of CAR activation on hepatic energy metabolism during physiological conditions, i.e. in mouse models not subjected to metabolic/nutritional stress. Gene expression profiling in the liver of CAR knockout and control mice on chow diet and treated with a CAR agonist highlighted CAR-mediated up-regulations of lipogenic genes, concomitant with neutral lipid accumulation. A strong CAR-mediated up-regulation of the patatin-like phospholipase domain-containing protein 3 (Pnpla3) was demonstrated. Pnpla3 is a gene whose polymorphism is associated with the pathogenesis of nonalcoholic fatty liver disease (NAFLD) development. This observation was confirmed in human hepatocytes treated with the antiepileptic drug and CAR activator, phenobarbital and in immortalized human hepatocytes treated with CITCO. Studying the molecular mechanisms controlling Pnpla3 gene expression, we demonstrated that CAR does not act by a direct regulation of Pnpla3 transcription or via the Liver X Receptor but may rather involve the transcription factor Carbohydrate Responsive Element-binding protein. These data provide new insights into the regulation by CAR of glycolytic and lipogenic genes and on pathogenesis of steatosis. This also raises the question concerning the impact of drugs and environmental contaminants in lipid-associated metabolic diseases., (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Published
- 2016
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18. Liver PPARα is crucial for whole-body fatty acid homeostasis and is protective against NAFLD.
- Author
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Montagner A, Polizzi A, Fouché E, Ducheix S, Lippi Y, Lasserre F, Barquissau V, Régnier M, Lukowicz C, Benhamed F, Iroz A, Bertrand-Michel J, Al Saati T, Cano P, Mselli-Lakhal L, Mithieux G, Rajas F, Lagarrigue S, Pineau T, Loiseau N, Postic C, Langin D, Wahli W, and Guillou H
- Subjects
- Adipocytes, Animals, Cytochrome P-450 Enzyme System genetics, Cytochrome P450 Family 4 genetics, Disease Models, Animal, Fasting, Fenofibrate pharmacology, Fibroblast Growth Factors biosynthesis, Gene Expression drug effects, Gene Expression Profiling, Homeostasis genetics, Hypoglycemia genetics, Hypolipidemic Agents pharmacology, Hypothermia genetics, Lipid Metabolism genetics, Lipolysis genetics, Male, Mice, Inbred C57BL, Mice, Knockout, Non-alcoholic Fatty Liver Disease metabolism, Overweight genetics, PPAR alpha metabolism, RNA, Messenger metabolism, Triglycerides metabolism, Aging physiology, Fatty Acids metabolism, Fibroblast Growth Factors genetics, Hepatocytes, Non-alcoholic Fatty Liver Disease genetics, PPAR alpha genetics
- Abstract
Objective: Peroxisome proliferator-activated receptor α (PPARα) is a nuclear receptor expressed in tissues with high oxidative activity that plays a central role in metabolism. In this work, we investigated the effect of hepatocyte PPARα on non-alcoholic fatty liver disease (NAFLD)., Design: We constructed a novel hepatocyte-specific PPARα knockout (Pparα(hep-/-)) mouse model. Using this novel model, we performed transcriptomic analysis following fenofibrate treatment. Next, we investigated which physiological challenges impact on PPARα. Moreover, we measured the contribution of hepatocytic PPARα activity to whole-body metabolism and fibroblast growth factor 21 production during fasting. Finally, we determined the influence of hepatocyte-specific PPARα deficiency in different models of steatosis and during ageing., Results: Hepatocyte PPARα deletion impaired fatty acid catabolism, resulting in hepatic lipid accumulation during fasting and in two preclinical models of steatosis. Fasting mice showed acute PPARα-dependent hepatocyte activity during early night, with correspondingly increased circulating free fatty acids, which could be further stimulated by adipocyte lipolysis. Fasting led to mild hypoglycaemia and hypothermia in Pparα(hep-/-) mice when compared with Pparα(-/-) mice implying a role of PPARα activity in non-hepatic tissues. In agreement with this observation, Pparα(-/-) mice became overweight during ageing while Pparα(hep-/-) remained lean. However, like Pparα(-/-) mice, Pparα(hep-/-) fed a standard diet developed hepatic steatosis in ageing., Conclusions: Altogether, these findings underscore the potential of hepatocyte PPARα as a drug target for NAFLD., (Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/)
- Published
- 2016
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19. Effect of obesity and metabolic syndrome on plasma oxysterols and fatty acids in human.
- Author
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Tremblay-Franco M, Zerbinati C, Pacelli A, Palmaccio G, Lubrano C, Ducheix S, Guillou H, and Iuliano L
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- Adult, Cholesterol blood, Cluster Analysis, Demography, Female, Humans, Male, Middle Aged, Oxidation-Reduction, Fatty Acids blood, Metabolic Syndrome blood, Metabolic Syndrome complications, Obesity blood, Obesity complications, Sterols blood
- Abstract
Background: Obesity and the related entity metabolic syndrome are characterized by altered lipid metabolism and associated with increased morbidity risk for cardiovascular disease and cancer. Oxysterols belong to a large family of cholesterol-derived molecules known to play crucial role in many signaling pathways underlying several diseases. Little is known on the potential effect of obesity and metabolic syndrome on oxysterols in human., Objectives: In this work, we questioned whether circulating oxysterols might be significantly altered in obese patients and in patients with metabolic syndrome. We also tested the potential correlation between circulating oxysterols and fatty acids., Methods: 60 obese patients and 75 patients with metabolic syndrome were enrolled in the study along with 210 age- and sex-matched healthy subjects, used as control group. Plasma oxysterols were analyzed by isotope dilution GC/MS, and plasma fatty acids profiling was assessed by gas chromatography coupled with flame ionization detection., Results: We found considerable differences in oxysterols profiling in the two disease groups that were gender-related. Compared to controls, males showed significant differences only in 4α- and 4β-hydroxycholesterol levels in obese and metabolic syndrome patients. In contrast, females showed consistent differences in 7-oxocholesterol, 4α-hydroxycholesterol, 25-hydroxycholesterol and triol. Concerning fatty acids, we found minor differences in the levels of these variables in males of the three groups. Significant changes were observed in plasma fatty acid profile of female patients with obesity or metabolic syndrome. We found significant correlations between various oxysterols and fatty acids. In particular, 4β-hydroxycholesterol, which is reduced in obesity and metabolic syndrome, correlated with a number of saturated and mono-unsaturated fatty acids that are end-products of de novo lipogenesis., Conclusions: Our data provide the first evidence that obesity and metabolic syndrome are associated with major, gender-specific, changes in circulating oxysterols and fatty acids. These findings suggest a metabolic link between oxysterols and fatty acids, and that oxysterols may contribute to the epidemic diseases associated with obesity and metabolic syndrome in female., (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Published
- 2015
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20. Adverse effects of long-term exposure to bisphenol A during adulthood leading to hyperglycaemia and hypercholesterolemia in mice.
- Author
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Marmugi A, Lasserre F, Beuzelin D, Ducheix S, Huc L, Polizzi A, Chetivaux M, Pineau T, Martin P, Guillou H, and Mselli-Lakhal L
- Subjects
- Age Factors, Animals, Biomarkers blood, Blood Glucose drug effects, Blood Glucose metabolism, Cholesterol blood, Gene Expression Profiling methods, Gene Expression Regulation drug effects, Hypercholesterolemia blood, Hypercholesterolemia diagnosis, Hyperglycemia blood, Hyperglycemia diagnosis, Insulin blood, Lipid Metabolism drug effects, Lipid Metabolism genetics, Liver drug effects, Liver metabolism, Male, Mice, Risk Assessment, Time Factors, Benzhydryl Compounds toxicity, Endocrine Disruptors toxicity, Hypercholesterolemia chemically induced, Hyperglycemia chemically induced, Phenols toxicity
- Abstract
Bisphenol A (BPA) is a suspected endocrine disruptor highly prevalent in our environment since it is used as monomer of polycarbonate plastics and epoxy resins. Recent epidemiological and animal studies have suggested that BPA exposure may influence the development of obesity and related pathologies such as type 2 diabetes, and cardiovascular diseases. However, experimental studies have often focused on short-term exposures. In this study, we investigated the effect of several months of BPA exposure on hepatic and plasma metabolic markers in adult mice. Male CD1 mice were exposed during 8 months to five different BPA doses below or equivalent to the current no observed adverse effect level (NOAEL: 5000 μg/kg/day) through drinking water. Plasma lipid profiles and liver transcriptomic analysis were performed in control and BPA-treated animals. We report a specific impact of BPA exposure on glycaemia, glucose tolerance and cholesterolemia. Consistent with the hypercholesterolemia in BPA-treated animals, RT-qPCR performed on hepatic mRNA from same animals demonstrated an overexpression of key genes involved in cholesterol biosynthesis, namely, Mvd, Lss Hmgcr, and Sqle. BPA also induced the expression of the sterol regulatory element-binding proteins 2, a master regulator of hepatic cholesterol biosynthesis. As shown by the plasma lathosterol to cholesterol ratio, a surrogate marker for cholesterol biosynthesis, whole body cholesterol de novo synthesis was also increased in BPA-exposed animals. These original results are consistent with many epidemiological studies reporting on a link between BPA exposure and the onset of cardiovascular diseases., (Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2014
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21. The liver X receptor: a master regulator of the gut-liver axis and a target for non alcoholic fatty liver disease.
- Author
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Ducheix S, Montagner A, Theodorou V, Ferrier L, and Guillou H
- Subjects
- Animals, Cholesterol metabolism, Fatty Acids biosynthesis, Humans, Lipid Metabolism, Liver X Receptors, Non-alcoholic Fatty Liver Disease, Fatty Liver metabolism, Gastrointestinal Tract metabolism, Liver metabolism, Orphan Nuclear Receptors metabolism
- Abstract
Since it is associated to the obesity epidemic, non alcoholic fatty liver disease (NAFLD) has become a major public health issue. NAFLD ranges from benign hepatic steatosis, i.e. abnormally elevated triglyceride accumulation, to non alcoholic steatohepatitis (NASH) that can lead to irreversible liver damages. The search for pharmacological and dietary approaches to treat or prevent NAFLD has pointed at nuclear receptors as sensible targets. Indeed, nuclear receptors are ligand-sensitive transcription factors that play a central role in hepatic lipid metabolism. Among nuclear receptors, the liver X receptor has been identified as an oxysterol receptor. It is involved in the control of various aspects of lipid metabolism that are reviewed in this manuscript. We highlight the role of LXR in the gut-liver axis and the studies that have provided a rationale for strategies specifically targeting the hepatic activity of LXR in NAFLD., (Copyright © 2013 Elsevier Inc. All rights reserved.)
- Published
- 2013
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22. Essential fatty acids deficiency promotes lipogenic gene expression and hepatic steatosis through the liver X receptor.
- Author
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Ducheix S, Montagner A, Polizzi A, Lasserre F, Marmugi A, Bertrand-Michel J, Podechard N, Al Saati T, Chétiveaux M, Baron S, Boué J, Dietrich G, Mselli-Lakhal L, Costet P, Lobaccaro JM, Pineau T, Theodorou V, Postic C, Martin PG, and Guillou H
- Subjects
- Animals, Cholesterol metabolism, Deficiency Diseases physiopathology, Dietary Fats pharmacology, Disease Models, Animal, Female, Gene Expression drug effects, Lipogenesis drug effects, Liver metabolism, Liver X Receptors, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Mice, Transgenic, Orphan Nuclear Receptors deficiency, Orphan Nuclear Receptors genetics, Transcription Factors physiology, Triglycerides metabolism, Up-Regulation physiology, Fatty Acids, Essential deficiency, Fatty Liver physiopathology, Gene Expression physiology, Lipogenesis genetics, Lipogenesis physiology, Orphan Nuclear Receptors physiology
- Abstract
Background & Aims: Nutrients influence non-alcoholic fatty liver disease. Essential fatty acids deficiency promotes various syndromes, including hepatic steatosis, through increased de novo lipogenesis. The mechanisms underlying such increased lipogenic response remain unidentified., Methods: We used wild type mice and mice lacking Liver X Receptors to perform a nutrigenomic study that aimed at examining the role of these transcription factors., Results: We showed that, in the absence of Liver X Receptors, essential fatty acids deficiency does not promote steatosis. Consistent with this, Liver X Receptors are required for the elevated expression of genes involved in lipogenesis in response to essential fatty acids deficiency., Conclusions: This work identifies, for the first time, the central role of Liver X Receptors in steatosis induced by essential fatty acids deficiency., (Copyright © 2013 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.)
- Published
- 2013
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23. A systems biology approach to the hepatic role of the oxysterol receptor LXR in the regulation of lipogenesis highlights a cross-talk with PPARα.
- Author
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Ducheix S, Podechard N, Lasserre F, Polizzi A, Pommier A, Murzilli S, Di Lisio C, D'Amore S, Bertrand-Michel J, Montagner A, Pineau T, Loiseau N, Lobaccaro JM, Martin PG, and Guillou H
- Subjects
- Animals, Cytochrome P-450 Enzyme System genetics, Cytochrome P450 Family 4, Fatty Acids metabolism, Fenofibrate pharmacology, Hydrocarbons, Fluorinated pharmacology, Ligands, Lipogenesis drug effects, Liver X Receptors, Male, Mice, Mice, Transgenic, Oligonucleotide Array Sequence Analysis, Orphan Nuclear Receptors agonists, Orphan Nuclear Receptors deficiency, PPAR alpha agonists, PPAR alpha deficiency, Protein Isoforms deficiency, Protein Isoforms metabolism, Sulfonamides pharmacology, Transcriptional Activation drug effects, Lipogenesis genetics, Liver cytology, Liver metabolism, Orphan Nuclear Receptors metabolism, PPAR alpha metabolism, Receptor Cross-Talk drug effects, Systems Biology
- Abstract
The Liver X Receptors (LXRs) α and β and the Peroxisome Proliferator-Activated Receptor α (PPARα) are transcription factors that belong to class II nuclear receptors. They drive the expression of genes involved in hepatic lipid homeostasis and therefore are important targets for the prevention and treatment of nonalcoholic fatty liver disease (NAFLD). LXRs and PPARα are regulated by endogenous ligands, oxysterols and fatty acid derived molecules, respectively. In the liver, pharmacological activation of LXRs leads to the over-expression of genes involved in de novo lipogenesis, while PPARα is critical for fatty acid catabolism in nutrient deprivation. Even if these two nuclear receptors seemed to play opposite parts, recent studies have highlighted that PPARα also influence the expression of genes involved in fatty acids synthesis. In this study, we used pharmacological approaches and genetically engineered mice to investigate the cross-talk between LXRs and PPARα in the regulation of genes responsible for lipogenesis. We first investigated the effect of T0901317 and fenofibrate, two synthetic agonists of LXRs and PPARα, respectively. As expected, T0901317 and fenofibrate induce expression of genes involved LXR-dependent and PPARα-dependent lipogenic responses. Considering such overlapping effect, we then tested whether LXR agonist may influence PPARα driven response and vice versa. We show that the lack of PPARα does not influence the effects of T0901317 on lipogenic genes expression. However, PPARα deficiency prevents the up-regulation of genes involved in ω-hydroxylation that are induced by the LXR agonist. In addition, over-expression of lipogenic genes in response to fenofibrate is decreased in LXR knockout mice as well as the expression of PPARα target genes involved in fatty acid oxidation. Altogether, our work provides in vivo evidence for a central interconnection between nuclear receptors that drive hepatic lipid metabolism in response to oxysterol and fatty acids., (Copyright © 2012 Elsevier Masson SAS. All rights reserved.)
- Published
- 2013
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24. Low doses of bisphenol A induce gene expression related to lipid synthesis and trigger triglyceride accumulation in adult mouse liver.
- Author
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Marmugi A, Ducheix S, Lasserre F, Polizzi A, Paris A, Priymenko N, Bertrand-Michel J, Pineau T, Guillou H, Martin PG, and Mselli-Lakhal L
- Subjects
- Animals, Benzhydryl Compounds, Gene Expression Profiling, Insulin blood, Lipid Metabolism, Liver metabolism, Male, Mice, Oligonucleotide Array Sequence Analysis, Estrogens, Non-Steroidal administration & dosage, Gene Expression drug effects, Lipids biosynthesis, Liver drug effects, Phenols administration & dosage
- Abstract
Unlabelled: Changes in lifestyle are suspected to have strongly influenced the current obesity epidemic. Based on recent experimental, clinical, and epidemiological work, it has been proposed that some food contaminants may exert damaging effects on endocrine and metabolic functions, thereby promoting obesity and associated metabolic diseases such as nonalcoholic fatty liver disease (NAFLD). In this work, we investigated the effect of one suspicious food contaminant, bisphenol A (BPA), in vivo. We used a transcriptomic approach in male CD1 mice exposed for 28 days to different doses of BPA (0, 5, 50, 500, and 5,000 μg/kg/day) through food contamination. Data analysis revealed a specific impact of low doses of BPA on the hepatic transcriptome, more particularly on genes involved in lipid synthesis. Strikingly, the effect of BPA on the expression of de novo lipogenesis followed a nonmonotonic dose-response curve, with more important effects at lower doses than at the higher dose. In addition to lipogenic enzymes (Acc, Fasn, Scd1), the expression of transcription factors such as liver X Receptor, the sterol regulatory element binding protein-1c, and the carbohydrate responsive element binding protein that govern the expression of lipogenic genes also followed a nonmonotonic dose-response curve in response to BPA. Consistent with an increased fatty acid biosynthesis, determination of fat in the liver showed an accumulation of cholesteryl esters and of triglycerides., Conclusion: Our work suggests that exposure to low BPA doses may influence de novo fatty acid synthesis through increased expression of lipogenic genes, thereby contributing to hepatic steatosis. Exposure to such contaminants should be carefully examined in the etiology of metabolic diseases such as NAFLD and nonalcoholic steatohepatitis., (Copyright © 2011 American Association for the Study of Liver Diseases.)
- Published
- 2012
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25. Key role of the ERK1/2 MAPK pathway in the transcriptional regulation of the Stearoyl-CoA Desaturase (SCD1) gene expression in response to leptin.
- Author
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Mauvoisin D, Prévost M, Ducheix S, Arnaud MP, and Mounier C
- Subjects
- Analysis of Variance, Blotting, Western, Cells, Cultured, Electrophoretic Mobility Shift Assay, Hep G2 Cells, Humans, Insulin pharmacology, Janus Kinase 2 metabolism, Promoter Regions, Genetic drug effects, Promoter Regions, Genetic physiology, Reverse Transcriptase Polymerase Chain Reaction, Stearoyl-CoA Desaturase metabolism, Gene Expression Regulation drug effects, Leptin pharmacology, Mitogen-Activated Protein Kinases physiology, Stearoyl-CoA Desaturase genetics, Transcription, Genetic drug effects, Transcription, Genetic physiology
- Abstract
Stearoyl-CoA Desaturase-1 (SCD1) is the rate limiting enzyme catalyzing the synthesis of monounsaturated fatty acids. Variation of SCD1 activity and the ratio of saturated to unsaturated fatty acids have been implicated in a variety of diseases including obesity, type II diabetes and cancers. In liver, many factors regulate SCD1 expression including dietary and hormonal factors such as insulin and leptin. We previously showed in hepatic cells that insulin acts through the PI3K and mTOR pathways to upregulate SCD1 expression. In the present study, using HepG2 cells, we characterized the signaling pathway mediating the leptin inhibitory response on SCD1 gene expression. We showed that leptin inhibits SCD1 at the transcriptional level. Inhibition of the ERK1/2 MAPK pathway with the PD98059 reverses the effect of leptin on SCD1 expression. Our data also demonstrated that the effect of leptin is entirely independent of the effect of insulin. Using the pharmaceutical inhibitors Ag490 and SL0101, we showed that the inhibitory effect of leptin is also mediated by the Janus Kinase 2 (Jak2) and p90RSK. EMSA and transfection experiments suggest a key role for the Sp1 transcription factor, which in turn may compete for the binding of other transcription factors such as AP-1, leading to the inhibition of SCD1 transcription. Taken together, our observations showed that, independently of insulin action, leptin exerts an inhibitory effect on SCD1 transcription via a signaling pathway implicating Jak2, ERK1/2, and p90RSK which probably targets the downstream transcription factor Sp1 on the SCD1 promoter., (Copyright 2010 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2010
- Full Text
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