1. Visualization of oxidative injury in the mouse kidney using selective superoxide anion fluorescent probes.
- Author
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Lv Y, Dan Cheng, Dongdong Su, Chen M, Yin BC, Yuan L, and Zhang XB
- Abstract
Drug-induced acute kidney injury (AKI), caused by renal drug metabolism, has been regarded as a main problem in clinical pharmacology and practice. However, due to the lack of effective biomarkers and noninvasive real-time tools, the early diagnosis of drug-induced AKI is still a crucial challenge. The superoxide anion (O
2 ˙- ), the preliminary reactive oxidative species, is closely related to drug-induced AKI. In this paper, we reported two new mitochondria-targeted fluorescent probes for investigating AKI via mapping the fluctuation of O2 ˙- with high sensitivity and selectivity by the combination of rational design and a probe-screening approach. Small-molecule fluorescent probes ( Naph-O2 ˙- and NIR-O2 ˙- ) with high accuracy and excellent selectivity were successfully applied to detect endogenously produced O2 ˙- in living cells and tissues by dual-model confocal imaging, and to trap the fluctuation of the O2 ˙- level during the drug-induced nephrotoxicity. Moreover, probe NIR-O2 ˙- was also used to elucidate the protective effects of l-carnitine (LC) against drug-induced nephrotoxicity for the first time. Therefore, these probes may be potential chemical tools for exploring the roles of O2 ˙- in complex nephrotoxicity disease systems.- Published
- 2018
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