1. Contribution of Human papillomavirus in neuroendocrine tumors from a series of 10,575 invasive cervical cancer cases.
- Author
-
Alejo M, Alemany L, Clavero O, Quiros B, Vighi S, Seoud M, Cheng-Yang C, Garland SM, Juanpere N, Lloreta J, Tous S, Klaustermeier JE, Quint W, Bosch FX, de Sanjosé S, and Lloveras B
- Subjects
- Adult, Aged, Cyclin-Dependent Kinase Inhibitor p16 genetics, DNA, Viral isolation & purification, Female, Genotype, Human papillomavirus 16 pathogenicity, Human papillomavirus 18 pathogenicity, Humans, Immunohistochemistry, Microscopy, Electron, Middle Aged, Neoplasm Invasiveness, Neuroendocrine Tumors ultrastructure, Polymerase Chain Reaction, Uterine Cervical Neoplasms pathology, Uterine Cervical Neoplasms virology, Neuroendocrine Tumors virology, Papillomaviridae pathogenicity, Papillomavirus Infections complications, Uterine Cervical Neoplasms complications
- Abstract
Aims: Neuroendocrine tumors (NET) of the cervix are rare tumors with a very aggressive course. The human papillomavirus (HPV) has been linked to its etiology. The objective of this study is to describe HPV prevalence and genotype distribution of NET., Methods and Results: Forty-nine tumors with histological neuroendocrine features were identified among 10,575 invasive cervical cancer (ICC) cases from an international study. HPV DNA detection was done using SPF10/DEIA /LiPA
25 system. Immunohistochemical (IHC) staining for neuroendocrine markers (chromogranin A, synaptophysin, CD56) and for p16INK4a as a surrogate for HPV transforming infection was performed. In 13 samples with negative IHC for all 3 neuroendocrine markers studied, it was possible to conduct electron microscopy (EM). NET represented 0.5% of the total ICC series and HPV was detected in 42 out of 49 samples (85.7%, 95%CI:72.8%,94.1%). HPV16 was the predominant type (54.8%), followed by HPV18 (40.5%). p16INK4a overexpression was observed in 38/44 cases (86.4%). Neuroendocrine IHC markers could be demonstrated in 24/37 (64.9%) cases. EM identified neuroendocrine granules in 8 samples with negative IHC markers., Conclusions: Our data confirms the association of cervical NET with HPV and p16INK4a overexpression. Specifically, HPV16 and 18 accounted together for over 95% of the HPV positive cases. Current HPV vaccines could largely prevent these aggressive tumors., (Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.) more...- Published
- 2018
- Full Text
- View/download PDF