1. Rice protein exerts a hypocholesterolemic effect through regulating cholesterol metabolism-related gene expression and enzyme activity in adult rats fed a cholesterol-enriched diet.
- Author
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Yang L, Han G, Liu QH, Wu Q, He HJ, Cheng CZ, and Duan YJ
- Subjects
- Acyl Coenzyme A genetics, Acyl Coenzyme A metabolism, Animals, Caseins pharmacology, Cholesterol 7-alpha-Hydroxylase genetics, Cholesterol 7-alpha-Hydroxylase metabolism, Diet, Gene Expression drug effects, Hypercholesterolemia genetics, Hypercholesterolemia metabolism, Hypercholesterolemia prevention & control, Lipid Metabolism genetics, Liver drug effects, Liver metabolism, Liver X Receptors, Male, Orphan Nuclear Receptors genetics, Orphan Nuclear Receptors metabolism, PPAR alpha genetics, PPAR alpha metabolism, RNA, Messenger metabolism, Rats, Rats, Wistar, Sterol O-Acyltransferase genetics, Sterol O-Acyltransferase metabolism, Sterol Regulatory Element Binding Proteins genetics, Sterol Regulatory Element Binding Proteins metabolism, Anticholesteremic Agents pharmacology, Cholesterol blood, Cholesterol, Dietary administration & dosage, Dietary Proteins pharmacology, Lipid Metabolism drug effects, Oryza chemistry, Plant Proteins pharmacology
- Abstract
The major aim of this study is to elucidate the hypocholesterolemic mechanism exerted by rice protein (RP) in adult rats under cholesterol-enriched dietary condition. Compared with casein, the cholesterol levels in plasma and the liver were significantly reduced by RP, accompanying significant inhibition of cholesterol absorption. RP increased the activity and mRNA level of cholesterol 7α-hydroxylase, whereas acyl-CoA:cholesterol acyltransferase activity and gene expression were significantly depressed with consumption of RP. Neither the activity nor gene expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase of RP differed from that of casein. The gene expression of the peroxisome proliferator-activated receptor α and liver X receptor α were significantly activated by consumption of RP. RP did not modify the mRNA level of sterol regulatory element-binding protein-2 with respect to casein. These results suggest RP can induce a cholesterol-lowering effect through modifying cholesterol metabolism-related gene expression and enzyme activity in adult rats.
- Published
- 2013
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