1. Scanning Switch-off Microscopy for Super-Resolution Fluorescence Imaging.
- Author
-
Gao Z, Hou S, Deng S, Liang L, Wang F, Guo L, Fang W, Li Q, Kang B, Chen HY, and Fan C
- Subjects
- Humans, DNA chemistry, Optical Imaging methods, Nanostructures chemistry, Microscopy, Fluorescence methods, Fluorescent Dyes chemistry, Microscopy, Confocal methods
- Abstract
Super-resolution (SR) microscopy provides a revolutionary optical imaging approach by breaking the diffraction limit of light, while the commonly required special instrumentation with complex optical setup hampers its popularity. Here, we present a scanning switch-off microscopy (SSM) concept that exploits the omnipresent switch-off response of fluorophores to enable super-resolution imaging using a commercial confocal microscope. We validated the SSM model with theoretical calculations and experiments. An imaging resolution of ∼100 nm was obtained for DNA origami nanostructures and cellular cytoskeletons using fluorescent labels of Alexa 405, Alexa 488, Cy3, and Atto 488. Notably, super-resolution imaging of live cells was realized with SSM, by employing a dronpa fluorescent protein as the fluorescent label. In principle, this SSM method can be applied to any excitation laser scanning-based microscope.
- Published
- 2024
- Full Text
- View/download PDF