1. Kre6 (yeast 1,6-β-transglycosylase) homolog, PhTGS, is essential for β-glucan synthesis in the haptophyte Pleurochrysis haptonemofera .
- Author
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Inukai M, Kobayashi N, Endo H, Asakawa K, Amano K, Yasuda Y, Cenci U, Colleoni C, Ball S, and Fujiwara S
- Abstract
Haptophytes synthesize unique β-glucans containing more β-1,6-linkages than β-1,3 linkages, as a storage polysaccharide. To understand the mechanism of the synthesis, we investigated the roles of Kre6 (yeast 1,6-β-transglycosylase) homologs, PhTGS, in the haptophyte Pleurochrysis haptonemofera . RNAi of PhTGS repressed β-glucan accumulation and simultaneously induced lipid production, suggesting that PhTGS is involved in β-glucan synthesis and that the knockdown leads to the alteration of the carbon metabolic flow. PhTGS was expressed more in light, where β-glucan was actively produced by photosynthesis, than in the dark. The crude extract of E . coli expressing PhKre6 demonstrated its activity to incorporate
14 C-UDP-glucose into β-glucan of P. haptonemofera . These findings suggest that PhTGS functions in storage β-glucan synthesis specifically in light, probably by producing the β-1,6-branch., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Inukai, Kobayashi, Endo, Asakawa, Amano, Yasuda, Cenci, Colleoni, Ball and Fujiwara.)- Published
- 2023
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