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Droughts evolve in space and time without following borders or pre-determined temporal constraints. Here, we present a new database of drought events built with a three-dimensional density-based clustering algorithm. The chosen approach is able to identify and characterize the spatio-temporal evolution of drought events, and it was tuned with a supervised approach against a set of past global droughts characterized independently by multiple drought experts. About 200 events were detected over Europein the period 1981-2020 using SPI-3 (3-month cumulated Standardized Precipitation Index) maps derived from the ECMWF (European Centre for Medium-range Weather Forecasts) 5th generation reanalysis (ERA5) precipitation. The largest European meteorological droughts during this period occurred in 1996, 2003, 2002 and 2018. A general agreement between the major events identified by the algorithm and drought impact records was found, as well as with previous datasets based on pre-defined regions., (© 2023. The Author(s).)

European forests are an important source for timber production, human welfare, income, protection and biodiversity. During the last two decades, Europe has experienced a number of droughts which have been exceptional within the last 500 years, both in terms of duration and intensity. These droughts seem to leave remarkable imprints on the mortality dynamics of European forests. However, systematic observations on tree decline, with emphasis on a single species, has been scarce so far so that our understanding of mortality dynamics and drought occurrence is still limited at a continental scale. Here, we make use of the ICP Forest crown defoliation dataset, permitting us to retrospectively monitor tree mortality for all major conifers, major broadleaves, as well as a pooled dataset of minor tree species in Europe. In total, we analysed more than three million observations gathered during the last 25 years and employed a high-resolution drought index which can assess soil moisture anomaly based on a hydrological water-balance and runoff model. We found overall and species-specific increasing trends in mortality rates, accompanied by decreasing soil moisture. A generalized linear mixed model identified a previous-year soil moisture anomaly as the most important driver of mortality patterns in conifers, but the response was not uniform across the numerous analysed plots. We conclude that mortality patterns in European forests are currently reaching a concerning upward trend which could be further accelerated by global change-type droughts in the near future., (© 2022 German Society for Plant Sciences, Royal Botanical Society of the Netherlands.)

Study Region: The Pampas region is located in the central-east part of Argentina, and is one of the most productive agricultural regions of the world under rainfed conditions., Study Focus: This study aims at examining how different Land Surface Models (LSMs) and satellite estimations reproduce daily surface and root zone soil moisture variability over 8 in-situ observation sites. The ability of the LSMs to detect dry and wet events is also evaluated., New Hydrological Insights for the Region: The surface and root zone soil moisture of the LSMs and the surface soil moisture of the ESA CCI (European Space Agency Climate Change Initiative, hereafter ESA-SM) show in general a good performance against the in-situ measurements. In particular, the BHOA (Balance Hidrológico Operativo para el Agro) shows the best representation of the soil moisture dynamic range and variability, and the GLDAS (Global Land Data Assimilation System)-Noah, ERA-Interim TESSEL (Tiled ECMWF's Scheme for Surface Exchanges over Land) and Global Drought Observatory (GDO)-LISFLOOD are able to adequately represent the soil moisture anomalies over the Pampas region. In addition to the LSM results, also the ESA-SM satellite estimated anomalies proved to be valuable. However, the LSMs and the ESA-SM have difficulties in reproducing the soil moisture frequency distributions. Based on this study, it is clear that accurate forcing data and soil parameters are critical to substantially improve the ability of LSMs to detect dry and wet events., Competing Interests: The authors report no declarations of interest., (© 2020 The Author(s).)

The temporal consistency of the fAPAR GEOV2 full time series (constituted by data derived from SPOT-VGT1/2 and PROBA-V) is analyzed against the single-sensor MODIS dataset, with a particular focus on the most recent fAPAR anomalies ( z -scores) produced from PROBA-V in the period 2014-2017. The intercomparison highlights a systematic overestimation of GEOV2 fAPAR z -scores when compared to MODIS fAPAR, likely related to the observed positive bias (over 90% of the domain) in the PROBA-V vs. SPOT-VGT1/2 relationship. A simple two-step harmonization procedure has been proposed to remove this discrepancy, based on two separate linear corrections of SPOT-VGT1/2 (2001-2013) and PROBA-V (2014-2017) data with respect to MODIS, followed by a time lag correction. The harmonized GEOV2 time series preserves the overall dynamic of fAPAR, while removing the sensor bias and improving the consistency with MODIS data. The fAPAR anomalies from the harmonized GEOV2 time series provide unbiased estimates of z -scores that are overall well correlated ( R  = 0.55 ± 0.25) with the MODIS fAPAR anomalies., (© 2019 The Authors.)

The purpose of this research was to explore the unstirred, ferroin-catalyzed Belousov-Zhabotinsky (BZ) reaction as an experimental model for the response of excitable media to small perturbations (slightly larger than the threshold for excitations). Following Showalter et al. (Showalter, K.; Noyes, R. M.; Turner, H. J.Am. Chem. Soc. 1979, 101, 7463-69), we used a positively biased silver electrode to release silver ions into a BZ reaction mixture, removing bromide ions and causing an excitation if sufficient bromide was removed. We found (1) a scaling region in which the delay before activation increased linearly as the size of the perturbation decreased, qualitatively consistent with but not fully explained by the Oregonator of Field et al. (Field, R. J.; Körõs, E.; Noyes, R. M. J. Am. Chem. Soc. 1972, 94, 8649-64); (2) evidence for a 10 s oligomerization time scale; and (3) that activations were always delayed until after the end of a pulse of current, with the delay essentially constant for sufficiently long pulses, an effect not seen in simple ODE models but consistent with the anomalously large current apparently required for activation (Showalter, K.; Noyes, R. M. J. Am. Chem. Soc. 1976, 98, 3730-31) and explainable by bromide transport. Overall, the BZ system appeared to be well-suited as an experimental prototype, despite its complexity.

The prototype experimental example of "spontaneous" pattern formation in an unstirred chemical medium is the oscillatory Belousov-Zhabotinsky (BZ) reaction: target patterns of outward-moving concentric rings are readily observed when the reaction is run in a thin layer in a Petri dish. In many experimental runs, new target centers appeared to form closer to pre-existing target centers than expected in a randomized model. Here we describe a simple direct test for the presence of temporal order in the spatiotemporal dynamics of target nucleation, and apply this test to detect significant temporal order in target formation in the ferroin-catalyzed BZ reaction. We also describe how mixing heterogeneity can generate temporal order, even in the absence of heterogeneous physical nucleating centers.

The objective of this study was to investigate the long-term effects of anti-retroviral protease inhibitors (PIs) on 2-deoxy-d -glucose (2-DG) transport in L6 cells in vitro. Exposure of L6 cells to saquinavir, ritonavir, indinavir and amprenavir resulted in significant increases in 2-DG transport using PI concentrations of 1-10 microM with continual exposure to PI. After removal of the PI for up to 48 h, 2-DG transport increases did not change and remained at pre-reversal levels. These changes in 2-DG transport were not related to stress-induced sugar transport or to apoptosis. The examination of glucose transporter (GLUT) 1, 3 or 4 translocation with subcellular fractionation indicated that insulin (i.e. 67 nM) could induce the translocation of all the GLUTs to the plasma membrane. Also, ritonavir (10 microM), which leads to a 2-fold increase in 2-DG transport, demonstrated increased GLUT (i.e. 1, 3 or 4) presence in the plasma membrane fraction, in the presence or absence of insulin. This increased 2-DG transport involved transporter presence in plasma membrane preparations and did not affect the ability of insulin to stimulate 2-DG transport with continual PI exposure. The mechanism(s) involved indicates ready reversibility of PI effects on transporters. The mechanism(s) why reversibility of PI-induced 2-DG transport was similar plus or minus PI was not apparent.

The objective of our research was to investigate the effects of the protease inhibitors ritonavir, saquinavir, and indinavir on triglyceride synthesis, lipolysis, insulin binding, and signaling in differentiating 3T3 L1 pre-adipocytes. Saquinavir, ritonavir, and indinavir all stimulated triglyceride (TG) synthesis. Additionally, all concentrations of protease inhibitors employed (i.e., 0.1 micro M to 10 micro M) significantly decreased insulin-stimulated TG synthesis. No effects of any of the protease inhibitors were observed either on basal lipolysis or after stimulation of lipolysis with 100 nM noradrenaline. Specific (125)I-insulin binding was observed to be decreased by exposure to all the protease inhibitors throughout the period of adipocyte phenotype development. This was mediated by indinavir through a receptor decrease and had no effect on receptor affinity. During differentiation with ritonavir (i.e., 1-11 days post addition of differentiating cocktail), insulin receptor substrate-1 (IRS-1) tyrosine phosphorylation was ascertained (day 11) and found to be decreased in the ritonavir exposed cells when compared with control cells. The results reported herein demonstrate protease inhibitor effects on basal TG synthesis while exhibiting decreased insulin-stimulated TG synthesis at physiological concentrations of protease inhibitors. These effects may be subsequent to decreased insulin binding and/or IRS-1 tyrosine phosphorylation.