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The increasing demand for water due to the escalation in population and aggressive agricultural activities for drinking and irrigation purposes in the rural areas of Ethiopia has put tremendous stress on water requirements. The Elgo River in southern Ethiopia is deteriorating due to sedimentation, soil erosion, stormwater runoff, and anthropogenic activities. Elgo village faces water shortages and a lack of safe drinking water. The purpose of this research was to identify the extent of pollution in Elgo River water using the Canadian Council of Ministers of the Environment (CCME), Water Quality Index (WQI), and Irrigation Water Quality Index (IWQI). A total of 12 water samples were collected from 3 river sampling sites for the dry and wet seasons to test the physicochemical and biological parameters. Results obtained were: turbidity (46.5-156) NTU, colour (103.65-606.5) TCU, EC (182-268) μS/cm, TDS (192.5-275.5) mg/l, TSS (680-2774) mg/l, Ca2+ (22-45) mg/l, Mg2+ (19.5-23.5) mg/l, Cl- (10.5-16.65) mg/l, and SO42- (17.18-47) mg/l for both the dry and wet seasons, respectively. The CCME WQI revealed that the overall results were 38.38 for the dry season and 36.6 for the wet season for drinking water parameters. The CCME WQI categorization indicates that the Elgo River water is classified as poor, with results ranging from 0 to 44. For irrigation purposes 10, parameters such as SAR, PS, PI, MAR, KI, RSC, EC, SSP, TH, and %Na were examined to compute indices using the IWQI model. The overall result of water quality indicated that IWQIs of 81.4 and 62.14 are good for the dry season and poor for the wet season, respectively. This research provides a thorough analysis through modelling to determine the suitability of water for different purposes for the tribal and backward communities of the area., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2023 The Authors.)

Cytochromes c are ubiquitous proteins, essential for life in most organisms. Their distinctive characteristic is the covalent attachment of heme to their polypeptide chain. This post-translational modification is performed by a dedicated protein system, which in many Gram-negative bacteria and plant mitochondria is a nine-protein apparatus (CcmA-I) called System I. Despite decades of study, mechanistic understanding of the protein-protein interactions in this highly complex maturation machinery is still lacking. Here, we focused on the interaction of CcmC, the protein that sources the heme cofactor, with CcmE, the pivotal component of System I responsible for the transfer of the heme to the apocytochrome. Using in silico analyses, we identified a putative interaction site between these two proteins (residues Asp 47 , Gln 50 , and Arg 55 on CcmC; Arg 73 , Asp 101 , and Glu 105 on CcmE), and we validated our findings by in vivo experiments in Escherichia coli Moreover, employing NMR spectroscopy, we examined whether a heme-binding site on CcmE contributes to this interaction and found that CcmC and CcmE associate via protein-protein rather than protein-heme contacts. The combination of in vivo site-directed mutagenesis studies and high-resolution structural techniques enabled us to determine at the residue level the mechanism for the formation of one of the key protein complexes for cytochrome c maturation by System I., (© 2018 Shevket et al.)

This paper presents an important new approach to improving the timeliness of Total Petroleum Hydrocarbon (TPH) analysis in the soil by Gas Chromatography - Flame Ionization Detector (GC-FID) using the CCME Canada-Wide Standard reference method. The Canada-Wide Standard (CWS) method is used for the analysis of petroleum hydrocarbon compounds across Canada. However, inter-laboratory application of this method for the analysis of TPH in the soil has often shown considerable variability in the results. This could be due, in part, to the different gas chromatography (GC) conditions, other steps involved in the method, as well as the soil properties. In addition, there are differences in the interpretation of the GC results, which impacts the determination of the effectiveness of remediation at hydrocarbon-contaminated sites. In this work, multivariate experimental design approach was used to develop and validate the analytical method for a faster quantitative analysis of TPH in (contaminated) soil. A fractional factorial design (fFD) was used to screen six factors to identify the most significant factors impacting the analysis. These factors included: injection volume (μL), injection temperature (°C), oven program (°C/min), detector temperature (°C), carrier gas flow rate (mL/min) and solvent ratio (v/v hexane/dichloromethane). The most important factors (carrier gas flow rate and oven program) were then optimized using a central composite response surface design. Robustness testing and validation of model compares favourably with the experimental results with percentage difference of 2.78% for the analysis time. This research successfully reduced the method's standard analytical time from 20 to 8min with all the carbon fractions eluting. The method was successfully applied for fast TPH analysis of Bunker C oil contaminated soil. A reduced analytical time would offer many benefits including an improved laboratory reporting times, and overall improved clean up efficiency. The method was successfully applied for the analysis of TPH of Bunker C oil in contaminated soil., (Crown Copyright © 2015. Published by Elsevier B.V. All rights reserved.)