1. Myopathic changes in murine skeletal muscle lacking synemin.
- Author
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García-Pelagio KP, Muriel J, O'Neill A, Desmond PF, Lovering RM, Lund L, Bond M, and Bloch RJ
- Subjects
- Animals, Biomechanical Phenomena, Costameres metabolism, Costameres pathology, Genotype, Intermediate Filament Proteins genetics, Male, Mice, Inbred C57BL, Mice, Knockout, Muscle Fatigue, Muscle Fibers, Fast-Twitch metabolism, Muscle Fibers, Fast-Twitch pathology, Muscle, Skeletal pathology, Muscle, Skeletal physiopathology, Muscular Diseases etiology, Muscular Diseases genetics, Muscular Diseases pathology, Muscular Diseases physiopathology, Phenotype, Running, Sarcolemma metabolism, Sarcolemma pathology, Intermediate Filament Proteins deficiency, Isometric Contraction, Muscle Strength, Muscle, Skeletal metabolism, Muscular Diseases metabolism
- Abstract
Diseases of striated muscle linked to intermediate filament (IF) proteins are associated with defects in the organization of the contractile apparatus and its links to costameres, which connect the sarcomeres to the cell membrane. Here we study the role in skeletal muscle of synemin, a type IV IF protein, by examining mice null for synemin (synm-null). Synm-null mice have a mild skeletal muscle phenotype. Tibialis anterior (TA) muscles show a significant decrease in mean fiber diameter, a decrease in twitch and tetanic force, and an increase in susceptibility to injury caused by lengthening contractions. Organization of proteins associated with the contractile apparatus and costameres is not significantly altered in the synm-null. Elastimetry of the sarcolemma and associated contractile apparatus in extensor digitorum longus myofibers reveals a reduction in tension consistent with an increase in sarcolemmal deformability. Although fatigue after repeated isometric contractions is more marked in TA muscles of synm-null mice, the ability of the mice to run uphill on a treadmill is similar to controls. Our results suggest that synemin contributes to linkage between costameres and the contractile apparatus and that the absence of synemin results in decreased fiber size and increased sarcolemmal deformability and susceptibility to injury. Thus synemin plays a moderate but distinct role in fast twitch skeletal muscle., (Copyright © 2015 the American Physiological Society.)
- Published
- 2015
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