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Ficus auriculata Lour. (Moraceae) is an underutilized wild edible fruit widely consumed for its nutritional properties. The present study aimed to determine the phytochemical composition and in vitro antioxidant, enzyme inhibitory, anti-inflammatory and anti-cancerous properties of the F. auriculata fruit extracts through in vitro digestion (oral, gastric and intestinal phases). The extracts were obtained by hot extraction and cold maceration methods using aqueous and methanolic solvents. Major phytoconstituents identified through LC-MS was subjected to molecular docking against the target proteins. The elemental analysis shows the presence of major elements; high levels of total phenolics (124.61 ± 0.82 mg gallic acid equivalent/g), flavonoids (76.38 ± 0.82 mg quercetin equivalent/g), vitamin E (32.48 ± 0.09 mg alpha-tocopherol equivalent/g), and carbohydrate (34.59 ± 0.45 mg glucose equivalent/g) in hot extracted methanolic undigested extract (HEM UD) and high level of total protein (124.71 ± 0.34 mg bovine serum albumin equivalent/g) in cold extracted methanolic undigested fruit extract were found. HEM UD showed high antioxidant activity in 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), 2,2-diphenyl-1-picryl-hydrazyl, and superoxide radical scavenging assays with IC 50 of 53.30 ± 0.57, 80.69 ± 0.12, and 65.47 ± 1.13 μg/mL, respectively. The HEM UD extract also potentially inhibited the enzyme activity of α-amylase, α-glucosidase, tyrosinase, and protein denaturation (IC 50 of 67.76 ± 1.22, 83.18 ± 1.23, 87.24 ± 1.15, and 65.76 ± 0.60 μg/mL). The most potent extract (HEM UD) was studied for its anticancer effects by MTT assay against the MCF-7 and HeLa cell lines and showed the IC 50 of 89.80 ± 0.56 and 60.76 ± 0.04 μg/mL, respectively. The LC-MS analysis elucidated ten phytoconstituents. Based on the molecular docking study, querciturone could potentially be an effective constituent in treating diabetes and inflammation-related issues. The findings indicated the ability of F. auriculata fruits as a promising functional food., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

A streamlined strategy for the one-pot synthesis of isoxazolone analogues has been developed through an acceptorless dehydrogenative annulation (ADA) pathway by employing new Ru(II) hydride complexes as effective catalysts. New Ru(II) complexes ( C1-C3 ) tailored with N̂O chelating carbazolone benzhydrazone ligands were synthesized and their formation was confirmed using analytical and spectral techniques including FT-IR and NMR. The structural configuration of the complexes featuring an octahedral geometry around the Ru(II) ion was precisely determined by single-crystal X-ray diffraction analysis. Further, the catalytic efficacy of the titled complexes has been established for the facile and productive synthesis of isoxazolone derivatives from a diverse range of benzyl alcohols, methyl acetoacetate/ethyl benzoylacetate and hydroxylamine hydrochloride, generating excellent yields of up to 93% under well-suited mild conditions with 1 mol % of catalyst loading. A sequence of time-dependent control experiments unveiled the ADA route, indicating the initial generation of an 4-methoxy benzaldehyde intermediate followed by 3-phenylisoxazol-5(4 H )-one, accompanied by the release of water and hydrogen as byproducts. Gram-scale synthesis of the compound indicates the industrial relevance of our synthetic strategy. A short synthesis of medicinally active androgen antagonist illustrates the utility of the present protocol.

Background: Researchers are increasingly accessing scientific articles through unauthorized websites like Sci-Hub. Sci-Hub contains retracted articles, including those which are not labelled as retracted, and this is a potential threat to academic research., Methods: This study analyses the extent of the availability of retracted articles within the Sci-Hub, particularly focusing on the presence of unlabeled retracted articles (URA) which may inadvertently be used in subsequent research, thus propagating flawed findings. The authors identified 16925 English-language research articles retracted between 2003 and 2022 indexed in the Web of Science and Scopus databases. These articles were cross-checked with Sci-Hub to ascertain whether they were appropriately labelled as retracted., Results: The investigation revealed that 84.83% of the retracted articles available on Sci-Hub do not have any indication of their retracted status. These URA could potentially be reused by researchers, unaware of their retracted status. The availability of URA in the field of health sciences is particularly high, which indicates a significant risk of their unintended use and further citation in future research., Conclusions: This study underscores the crucial need for stringent implementation of regulatory measures on retraction suggested by the Committee on Publication Ethics (COPE) or newly published National Information Standards Organization (NISO) recommendations.

Background: Datura stramonium L., a wild-growing herb, has been traditionally used to treat various ailments, including toothache, asthma, rheumatism, epilepsy, and alopecia. Scientific evidence supports its anticancer, anti-inflammatory, anti-asthmatic, anticholinergic, antifungal, and antibacterial properties., Aim: This study aimed to isolate, characterize, and identify the most potent anticandidal compounds inhibiting the growth of Candida spp., while also predicting their drug-likeness and toxicity profiles., Method: The anticandidal activity of D. stramonium leaf extracts was assessed using the Agar well-diffusion method and minimum inhibitory concentration (MIC) was determined by the broth dilution method. The most active extract was selected for column chromatography. Different fractions were collected and screened against pathogenic Candida spp. The most active fraction was subjected to Gas chromatography-Mass spectrometry (GC-MS), Fourier Transform-Infrared Spectroscopy (FT-IR), and Nuclear Magnetic Resonance (NMR) analysis. Additionally, computational tools such as molecular docking and ADMET prediction provided further insights into the molecular interactions between the target enzymes., Results: In vitro anticandidal activity demonstrated that the ethyl acetate extract exhibited significant activity against human pathogenic Candida spp., with the highest zones of inhibition against Candida guilliermondii (20.33 ± 0.56 mm), Candida tropicalis (16.33 ± 0.58 mm), and Candida albicans (14.66 ± 1.05 mm), with a minimum inhibitory concentration (MIC) value of 25 μg/ml. Additionally, the most potent fraction (F8) obtained from the Column revealed significant anticandidal activity. GC-MS analysis of the F8 fraction indicated the presence of 23 compounds, with the major compounds being Phthalic acid, di (2-propylpentyl) ester (Compound 1), Pentadecane (Compound 2), Octadecane (Compound 3), Benzoic acid, 3-Amino-5-Hydroxy-, Methyl ester (Compound 4), and 1,2-Benzenedicarboxylic acid, bis (2-ethylhexyl) ester (Compound 5). This study reports all 23 compounds from D. stramonium for the first time. Furthermore, NMR studies confirmed the presence of Phthalic acid, di (2-propylpentyl) ester as the most abundant compound, designated as compound 1. Finally, docking analysis revealed that compound 1 showed good binding affinities for the tested enzymes, with the highest binding scores of -7.084 kcal/mol and -7.030 kcal/mol with Lanosterol 14-alpha demethylase (PDB ID: 5JLC, 5TZ1). The results of the in silico pharmacokinetic and drug-likeness properties indicated that compound 1 is a potential anticandidal drug candidate., Conclusion: This study highlights that 23 compounds were reported from the leaf extract of D. stramonium for the first time. The findings suggest that compound 1 can be considered a new anticandidal drug candidate., Competing Interests: Declaration of competing interest The authors declare that there are no conflicts of interests., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

The present research aims to develop a Ca-Zn ion-incorporated surface functionalized 3D Ti cancellous bone scaffold for bone defect repair. The scaffold is designed to mimic human cancellous bone architecture through selective laser melting-based additive manufacturing. The chemical-based surface modification approach employed here created a Ca and Zn ions incorporated nano-porous surface layer with enhanced surface roughness and hydrophilicity. The modified biomimetic scaffold improved the corrosion resistance behaviour with I CORR and E CORR values of 0.174 mA and 0.0097 V respectively. It is learned that incorporating Zn as ZnO over the scaffold has antibacterial activity against Staphylococcus aureus and Escherichia coli. The cellular response of MG-63 to the modified scaffold was evaluated through in-vitro studies which focus on the cytocompatible properties. The intra-osseous biomimetic Ti-Na-Ca:Zn 3D scaffold revealed significant improvement in the osseointegration capabilities in terms of bone mineral density (BMD) and bone volume/total volume (BV/TV) in the rabbit model. The osseointegration potential at the Ti-Na-Ca:Zn interface was evidenced by histological analysis and micro-CT imaging. In addition to this, the remarkable upregulation of osteogenic genes such as OCN, COL1A1, OPN, ALP, RUNX2, and OSX evidences the dynamics of the osseointegration process at each surgical period. This Ca and Zn surface functionalised porous architecture of the 3D Ti cancellous bone scaffold with enhanced biological response and bone integration can potentially give insights into implant customisation along with improved clinical outcomes., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the present work., (Copyright © 2024. Published by Elsevier B.V.)

The utilization of the density functional theory (DFT) methodology has developed as a highly efficient method for investigating molecular structure and vibrational spectra, and it is increasingly being employed in various applications relating to biological systems. This study focuses on conducting investigations, both experimental and computed, to analyze the molecular structure, electronic properties and features of (E)-4-(((9H-purin-6-yl)imino)methyl)-2-methoxyphenol (ANVA). The expression ANVA should be rewritten as follows: the compound is a derivative of adenine (primary amine), specifically a vanillin (aldehyde). The present study reports the synthesis, characterization, DFT, docking and antimicrobial activity of ANVA. The optimization of the molecular structure was conducted, and the determination of its structural features was performed using DFT with the B3LYP/cc-pVDZ method. The vibrational assignments were determined in detail by analyzing the potential energy distribution. A strong correlation was observed between the spectra that were observed and the spectra that were calculated. The calculation of intramolecular charge transfer was performed using natural bond orbital analysis. In addition, several computational methods were employed, including highest occupied molecular orbital-least unoccupied molecular orbital analysis, molecular electrostatic potential calculations, non-linear optical, reduced density gradient, localization orbital locator and electron localization function analysis. This paper examines the present use of adenine derivatives in combatting bacterial and fungal infections, as well as the inclusion of spectral and quantum chemical calculations in the discussion.Communicated by Ramaswamy H. Sarma.

Rationale: Anabolic steroids, also known as anabolic-androgenic steroids (AAS), encompass steroidal androgens such as testosterone, as well as synthetic counterparts with similar structures and effects. The misuse of AAS has increased over the years, leading to ethical and welfare concerns in sports. The World Anti-Doping Agency (WADA) and the International Federation for Equestrian Sports (FEI) have banned AAS in relevant sports. Methandienone is one of the most identified anabolic androgenic steroids in sports drug testing, Therefore, reliable detection methods are crucial for effective doping control and maintaining the integrity of the sports., Methods: This study explores the use of homogenized camel liver for detecting methandienone metabolites in camels. The biotransformation pathways of methandienone in homogenized camel liver tissues are analyzed using Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS) to identify and characterize the phase I and phase II metabolites. Chromatographic separation was achieved using a Thermo-Hypersil C18 column., Results: The study has identified 11 methandienone metabolites (M1-M11), this includes 10 phase I and one phase II metabolite. A glucuronic acid conjugate of methandienone was observed in this study, but no sulfonic acid conjugations were found. The metabolites and their possible chemical structures, along with their fragmentation patterns are confirmed using MSMS (MS2) experiments in data-independent acquisition (DIA) mode., Conclusions: These findings serve as a vital tool for the rapid detection of methandienone, combating its illicit use in camel racing. Comprehensive screenings covering both the parent drug and its metabolites are recommended to improve detection accuracy and ensure regulatory compliance in sports doping. Future research should explore methandienone's metabolite profile in administered camel samples., (© 2024 John Wiley & Sons Ltd.)

Biodegradation of microplastics (MPs) through microalgal strains would be of eco-friendly approach for significant pollution abatement. Polystyrene (PS) is a major contaminant in the marine environment; however studies on marine microalgal degradation of PS MPs have been very limited. In the present study, six marine microalgal strains viz. Picochlorum maculatum, Dunaliella salina, Amphora sp., Navicula sp., Synechocystis sp. and Limnospira indica were investigated for their ability to degrade PS MPs for the incubation period of 45 days. Results from weight reduction, ATR-FTIR, SEM, and molecular docking analysis confirmed that microalgae formed biofilms on PS MPs, causing structural changes, and laccase-driven enzymatic breakdown. A maximum weight loss of 23.2 ± 0.21% and a minimum of 11.3 ± 0.026% were caused by the colonized microalgae Synechocystis sp. and Amphora sp. respectively. The study indicated that a higher reduction rate was observed in the Synechocystis sp. Treated PS MPs with a rate of 0.0058 g/day and a lower half-life of 119.34 days. SEM analysis showed that microalgae caused pits, erosion, and damage to the PS film. ATR-FTIR confirmed the chemical modifications and proved biodegradation. Laccase enzyme activity was higher in Synechocystis sp., and molecular docking showed the laccase interaction with the derivatives of PS, elucidating the breakdown process. This study highlights the potential of microalgae for eco-friendly microplastic degradation and paves the way for future research on the by-products of this process. Exploring the ecological impact of by-products and optimizing scalable methods can further enhance the sustainability and practical applications of this promising solution., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Background: Drought is one of main critical factors that limits sugarcane productivity and juice quality in tropical regions. The unprecedented changes in climate such as monsoon failure, increase in temperature and other factors warrant the need for development of stress tolerant cultivars to sustain sugar production. Plant Nuclear factor (NF-Y) is one of the major classes of transcription factors that have a major role in plant development and abiotic stress response. In our previous studies, we found that under drought conditions, the nuclear factor NF-YB2 was highly expressed in Erianthus arundinaceus, an abiotic stress tolerant wild genus of Saccharum species. In this study, the coding sequence of NF-YB2 gene was isolated from Erianthus arundinaceus and overexpressed in sugarcane to develop drought tolerant lines. RESULTS : EaNF-YB2 overexpressing sugarcane (OE) lines had higher relative water content, chlorophyll content and photosynthetic efficiency compared to non-transgenic (NT) control. In addition, overexpressing lines had higher activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR), and higher proline content, lower malondialdehyde (MDA) and peroxide (H 2 O 2 ) contents. The expression studies revealed that EaNF-YB2 expression was significantly higher in OE lines than NT control under drought stress. The OE lines had an elevated expression of abiotic stress responsive genes such as BRICK, HSP 70, DREB2, EDH45, and LEA3. The morphological analysis revealed that OE lines exhibited less wilting than NT under drought conditions., Conclusion: This study provides insights into the role of the EaNF-YB2 gene in drought tolerance in sugarcane. Based on the findings of this study, the EaNF-YB2 gene can be potentially exploited to produce drought tolerant sugarcane cultivars to sustain sugarcane production under water deficit conditions., Competing Interests: Declarations. Ethics approval and consent to participate: The Saccharum spp. Hybrid (Co 86032) used in this study was developed at the ICAR-Sugarcane Breeding Institute, Coimbatore, India, a government-funded institute. The experimental material involved follows institutional, national, and international guidelines and legislation. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2024. The Author(s).)

Lichen-associated endophytic Actinobacteria, particularly Streptomyces species, are recognized for their production of bioactive secondary metabolites with significant pharmaceutical potential. With the escalating prevalence of diseases, Streptomyces species are being investigated for its natural source of antimicrobial compounds for new antibiotics. This study focuses on the bioactive properties of secondary metabolites from lichen-associated endophytic Actinobacteria, focusing on Streptomyces glaucescens NTSB-37 isolated form lichen, Parmotrema perlatum (Huds.) M. Choisy of Kolli hills, Tamil Nadu, India. Among 54 Actinobacterial isolates (NTSB-1 to NTSB-54) collected from various regions in Tamil Nadu, Streptomyces glaucescen NTSB-37 demonstrated notable antibacterial and anti-dermatophytic activities against multidrug-resistant bacteria and dermatophytes, including Trichomonas rubrum and Microsporum canis. The morphological and molecular (16S rRNA gene sequencing) characterization confirmed its identity. Secondary metabolites screening via FTIR and GC-MS revealed bioactive compounds in crude extracts and exhibited increased antibacterial activity with increasing dosage volume and notable MIC values. Partial purification was achieved through TLC and HPTLC fingerprinting. Further investigation shows Streptomyces glaucescens NTSB-37, an endophytic actinobacteria, is a prolific producer of bioactive secondary metabolites with effective antimicrobial agents amid the growing challenge of drug resistance and suggesting biotechnological applications in human health. The findings furthermore emphasize the important of exploring novel ecosystem, particularly lichens with abundant microhabitats within and in hilly regions for developing bioactive compounds with therapeutic potential., Competing Interests: Declarations. Conflict of interest: The authors declare no competing interests. Ethical Approval: Not Applicable. Consent for Publication: All authors agreed to publish this manuscript in this journal., (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

Background: Tapentadol hydrochloride is a potent analgesic commonly used to manage moderate to severe pain. Rapidly dissolving tablets of Tapentadol offer a significant advantage in enhancing patient compliance by providing quick pain relief. The development of fast-dissolving tablets (FDTs) requires careful consideration of formulation parameters to achieve optimal disintegration and dissolution profiles. In this study, the aim was to fabricate Tapentadol FDTs by selecting suitable super disintegrating agents such as croscarmellose sodium and crospovidone, which serve as two independent variables. The direct compression method was employed to formulate nine different Tapentadol hydrochloride formulations (TH1 to TH9)., Materials and Methods: The study utilized Design-Expert® software version 13.0 and the Response Surface Methodology (RSM) for the optimization of Tapentadol FDTs. The formulations were prepared using the direct compression method with varying concentrations of the super disintegrants, croscarmellose sodium, and crospovidone. The primary response variables considered in this optimization study included disintegration time (Y1), percentage drug release at 15 minutes (Q15, Y2), and percentage drug release at 30 minutes (Q30, Y3). All pre-compressional and postcompressional parameters were evaluated for each formulation, along with in vitro dissolution studies. Furthermore, DD Solver, a statistical tool, was employed to determine the kinetics of drug release and the release order mechanism based on regression coefficient value (r²), Akaike Information Criterion (AIC), and Model Selection Criteria (MSC)., Results: The evaluation studies indicated that the TH5 formulation exhibited the most rapid disintegration time and the highest drug release percentage within the specified time frame. The super disintegrants used demonstrated a significant impact on the response variables, notably enhancing the solubility and dissolution rate of Tapentadol hydrochloride. Based on the exponent release (n) value, the study concluded that the TH5 formulation followed a first-order release kinetics and Fickian diffusion mechanism for drug release. Stability studies were performed following the International Council for Harmonization (ICH) guidelines to assess the shelf-life of the optimized formulation. The ANOVA data revealed that the p-value was greater than 0.05, indicating no significant differences during the storage period. Additionally, a similarity factor (f2) analysis was conducted to compare the optimized formulation with the marketed formulation (Tydol 100 mg)., Discussion: The findings highlight the crucial role of super disintegrants in fast-dissolving tablet formulation, significantly impacting disintegration time and dissolution profile. The TH5 formulation excelled in rapid disintegration and drug release, optimized using RSM and Design-Expert software, with statistical analysis confirming the Fickian diffusion mechanism for drug release., Conclusion: The study successfully developed and optimized Tapentadol fast-dissolving tablets using direct compression and response surface methodology. The TH5 formulation showed rapid disintegration and optimal drug release, with stability confirmed under ICH conditions. This highlights the importance of super disintegrants in FDT formulation for rapid action and patient compliance., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)

Kombucha is fermented and produced with a biofilm called a symbiotic culture of bacteria and yeast, which is drunk all over the world for its beneficial effects on human health and energy levels. The metagenomic study of kombucha frequently detected microorganisms in proteobacteria, firmicutes, and actinobacteria. And also, yeast and fungi are Ascomycota and Basidiomycota is present in green leaf and sugarcane juice fermented kombucha. The kombucha extracts' biological activities were assessed using pH, total phenolic content, antioxidant, antibacterial, and anticancer activity. Fermentation may enhance biological activity and the generation of bioactive substances. These results showed the pH -3.1 ± 0.2 and TPC -0.721 μg/mL of gallic acid equivalent. The antioxidant radicals scavenging activity of kombucha was evaluated by DPPH, ABTS, H 2 O 2 and TAC. The bioactive chemicals identified by FT-IR and HR-LC/MS analysis of Kombucha totaled 45 components. The identified compounds were further move on to perform molecular docking study against gastric cancer target proteins 4H9M, 2DQ7 and 1TVO are binding with Nequinate compounds showing best LibDock scores 105.12, 114.49, and 108.97. So, this study suggests that knowledge can potentially active bioactive compounds are present in kombucha and it's stimulated the mechanism of gastrointestinal transit. Additionally, the metagenomic analysis gives strength to understand the bacterial and fungal distribution and its molecular mechanism from Kombucha., (© The Author(s) 2024. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.)

The structures of the anticancer drug compounds satraplatin and picoplatin are optimized, and theoretical studies done by Density Functional Theory (DFT) to investigate the optical properties, global chemical descriptors, and band gap were calculated using the obtained Frontier molecular orbital values. The theoretically obtained data of the platinum drugs found to possess low energy gap values and low chemical hardness with high chemical softness describe that the compounds are chemically stable and most reactive. Using the molecular docking process, the binding mechanisms of the satraplatin and picoplatin drugs with cancer DNA structures were studied, and the results were analyzed. For the two drug compounds examined, satraplatin has shown the least binding energies at -5.06kcal/mol compared to picoplatin at -2.69 kcal/mol, and conformations with root mean square deviation (RMSD) values by DNA structures are less than or equal to 2.00 Å in targeting the specific DNA target residues of the reference bound ligand. The grid box was generated using the selective grid parameters using the Autodock 4.2 program. The drug molecules found to have the least binding energy value with the lowest inhibition constant implicate the good docking score towards the DNA structure by the docking studies. DFT studies also show good structural properties and chemical reactivity. From the obtained results, satraplatin and picoplatin were found to have good chemical descriptors and good binding affinity and are best suited for biological molecular targets., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Plastic debris in coastal environments usually undergoes weathering due to various environmental conditions. However, the weathering effects on exposed and shaded sides of the same plastics are underexplored. In this study, 1573 plastic fragments were collected from 15 coastal sites worldwide between December 2021 and December 2022, and weathering experiments were conducted outdoors. The field investigation showed significant two-sided weathering differences of plastic fragments. The weathering morphology included biota, cracks, delamination, discoloration, etc. The weathering degree was assessed with three metrics, i.e., line density (0-58 mm/mm 2 ), surface loss (0-92 %), and texture index (0-2). The 3D magnitudes of these three metrics revealed the two-sided weathering differences of plastic fragments. Specifically, 43 % of the samples had magnitudes > 5, indicating significant differences. Outdoor simulations suggested that sun-exposed sides developed more cracks, pores, and bubbles, while shaded sides remained smoother. After 12 months, the line density increased from 2.85 to 9.23 mm/mm² for polyethylene (PE) and 4.16-8.47 mm/mm² for polypropylene (PP) (p < 0.05). The carbonyl index increased from 0.50 to 1.70 (PE), from 0.18 to 1.10 (PP), and from 0.45 to 1.57 (polyvinyl chloride). This increase indicated oxidative degradation on sun-exposed sides. Our results highlighted the uneven degree of weathering on both sides of the same plastic fragment due to different environmental factors. The study provided critical insights for creating more accurate models to predict plastic degradation, which will help inform global strategies to reduce plastic pollution., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Microplastics (MPs, <5 mm) are widespread in coastal ecosystems and pose a growing global concern; however, their presence in deep-sea environments remains underexplored, especially in the Indian region. This study addresses this gap by providing the first comprehensive documentation of MPs in the Central Indian Ocean Basin (CIOB) at a depth of 5000 m, marking the initial effort to assess their presence and abundance in deep-sea core samples. The study investigated the MP concentration, composition and potential sources, revealing a size range between 10 μm and 4900 μm, with average abundances recorded at BC20 (10.2 ± 6.2 MPs/g), the PRZ (6.4 ± 5.0 MPs/g), and the IRZ (4.5 ± 0.8 MPs/g). Fibres constituted 54.9 % of the MPs, primarily blue, black, and red, and the predominant polymer was polyacetylene (50 %), followed by polyvinyl alcohol and polyvinyl chloride. These findings highlight the significant presence of MPs in the deep sea, underscoring the need for a better understanding of their transport and deposition mechanisms. This research is vital for shaping conservation strategies and policies aimed at mitigating MP pollution in marine ecosystems, emphasizing the urgency for further investigations in this region., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Dineshram Ramadoss reports financial support was provided by Government of India Ministry of Earth Sciences. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

A facile, cost-effective, and sustainable synthesis of substituted triazines from primary alcohols by newly synthesized nickel pincer-type complexes ( 1 - 3 ) has been described. Herein, we report the synthesis of a set of three well-defined Ni(II) O^N^O pincer-type complexes, structurally characterized by analytical, spectral, and X-ray diffraction techniques. Further, the nickel complexes are explored as efficient catalysts (4 mol %) for the construction of 2,4,6-substituted 1,3,5-triazines from readily available alcohols via an acceptorless dehydrogenative coupling (ADC) strategy. A wide range of substituted triazine derivatives (33 examples) has been synthesized from the coupling of alcohols and benzamidine/guanidine hydrochloride with a maximum isolated yield of 92% under mild conditions, with eco-friendly H 2 O and H 2 gas as the only byproducts. A plausible mechanism has been proposed based on a sequence of control experiments. Interestingly, the short synthesis of the antiulcer drug irsogladine and the large-scale synthesis of 2,4-diphenyl-6-( p -tolyl)-1,3,5-triazine highlight the convenience of the current methodology.

The research article details the synthesis of chalcone-chromone-based scaffolds via multicomponent reactions. These compounds were characterized using conventional spectroscopic methods, including NMR ( 1 H and 13 C), FT-IR, and HR-MS. Among the synthesized scaffolds, AZBNPy stood out, exhibiting exceptional DNA and protein targeting capabilities with superior binding parameters. Molecular docking studies indicated that AZBNPy has potential as a potent anticancer agent and a probe for cancer cell imaging. The findings showed that AZBNPy effectively inhibited cell proliferation and induced cell death by targeting HER-2, PARP-2, and HFR in MCF-7 cells. Additionally, in vitro fluorescence imaging studies confirmed AZBNPy's specificity for cancer cell receptors, displaying strong fluorescence in human breast cancer tissues. The clinical application of AZBNPy as an optical imaging agent holds significant promise in aiding surgeons with the precise identification and removal of cancerous tissues, potentially improving patient outcomes and survival rates., Competing Interests: Declarations. Competing Interests: The authors declare no competing interests. Supplementary Information: Supplementary information document contains detailed synthetic methods of chalcone and their derivatives and the techniques used in spectral data, absorption spectra and graphical plot for the interaction of scaffold with DNA and BSA associated with this research manuscript can be retrieving in the online version., (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

In the present study, lanthanum oxytellurate (LOT) samples with varying La : Te ratios are successfully synthesized using a simple hydrothermal method that has enormous advantages. The prepared samples crystallize in a La 2 O 6 Te composite phase with an orthorhombic crystal system. A nanorod-like morphology is observed for each sample, and the presence of constituent elements is verified from EDX results. Chemical compositions and their oxidation states are obtained from XPS studies. Optical studies of the materials demonstrated band gap values between 2.65 and 2.78 eV, confirming the LOT samples' semiconducting nature. Broad photoluminescence (PL) spectra with peaks ranging between 650 and 750 nm were observed, and a red shift occurred by increasing the Te concentration in the samples. Overall, the samples exhibit good photoresponse characteristics under dark and light conditions with high I on / I off ratios and sensitivity values. These parameters confirm the potential of LOT materials to stand out as an alternative for susceptible and effective photodetector devices. Among the samples, LOT-2 showed 10.64 μA W -1 responsivity, 5.8 × 10 7 Jones of detectivity, and a rise and fall time of 16.25 and 23.13 s, respectively, which was the best photodetection performance compared to the other two samples. Results from the nonlinear optical (NLO) Z -scan study demonstrated the RSA behavior and valley-peak configuration with positive n 2 values corresponding to the self-focusing effect. The NLO characteristics of the materials are governed by the two-photon absorption (2PA) mechanism. Here, the LOT-2 sample displayed comparatively better results than the other two. The third-order nonlinear optical NLO susceptibility χ (3) values suggest that LOT materials have the potential to become a new candidate for various NLO applications in the coming days.

In recent years, several physicochemical methods have been proposed for decolourising textile dyes; however, few have been adopted by the textile industry because of factors such as high cost, low efficiency, and limited applicability to a wide range of dyes. The current study focuses on synthesising algae-mediated Cu and CuO nanocatalysts (Alg-Cu and Alg-CuO) using natural waste materials from green algae. The synthesised Alg-CuO nanocatalyst was characterised and confirmed using SEM, TEM, UV, FT-IR, XRD, XPS, GC-MS, and TGA. An innovative and efficient technique for decolourising dyes through aerobic oxidation was implemented in industrial wastewater treatment. Various hydroxylamine substrates were successfully transformed into the desired aldehydes using an Alg-CuO nanocatalyst. In the process of aerobic oxidation, 2-(2-amino-ethyl)-aminoethanol can be converted into 2-(2-amino-ethyl)acetaldehyde, resulting in 96% product conversion within 4 min. In addition, the synthesised Alg-CuO nanocatalyst was used to investigate the dye decolourisation process using CBB G250 dye. The Alg-CuO nanocatalyst exhibited excellent decolourisation properties; for 20 min, 85% decolourisation of the CBB G250 dye was achieved. As a result, green synthesis is a viable medium for producing Alg-CuO nanocatalysts with high bond energies for dye decolourisation. Finally, the dye and Alg-CuO nanocatalyst was separated and reused for the following process. This method has been used for industrial wastewater treatment., Competing Interests: Declarations. Competing interests: The authors declare no competing interests., (© 2024. The Author(s).)

Background/Objective: Indwelling intrauterine contraceptive devices (IUDs) have surfaces that facilitate the attachment of Candida spp., creating a suitable environment for biofilm formation. Due to this, vulvovaginal candidiasis (VVC) is frequently linked to IUD usage, necessitating the prompt removal of these devices for effective treatment. In this study, we evaluated the susceptibility of antimicrobial peptides in vitro against biofilm forming, Amphotericin B (MIC50 > 2 mg L -1 ) resistant Candida krusei and Candida tropicalis isolated from IUD users who had signs of vaginal candidiasis (hemorrhage, pelvic pain, inflammation, itching, and vaginal discharge). Three antimicrobial peptides, namely, epinecidin-1 (epi-1) and its two variants, namely, variant-1 (Var-1) and variant-2 (Var-2), which were reported to have enhanced antibacterial activity were tested against IUD isolates ( C. krusei and C. tropicalis ) with pathogenic form of Candida albicans as control. Variants of epi-1, namely, Var-1 and Var-2 were created by substituting lysine in the place of histidine and alanine. Methods: The antimicrobial activity was measured using the microbroth dilution method to determine the minimum inhibitory concentration (MIC) of peptides against C. albicans , C. krusei and C. tropicalis . The MIC of each peptide was used for biofilm assay by Crystal violet staining, Scanning Electron Microscopy, and Reactive Oxygen Species (ROS) assay. To find the possible mechanism of anti-biofilm activity by the peptides, their ability to interact with Candida spp. cell membrane proteins such as Exo-β-(1,3)-Glucanase, Secreted Aspartic Proteinase (Sap) 1, and N-terminal Domain Adhesin: Als 9-2 were determined through PatchDock. Results: The MIC values of peptides: epi-1, var-1 and var-2 against C. albicans are 128 μg mL -1 , 64 μg mL -1 and 32 μg mL -1 , C. tropicalis are 256 μg mL -1 , 64 μg mL -1, and 32 μg mL -1 and C. krusei are 128 µg mL -1 , 128 µg mL -1 and 64 µg mL -1 , respectively. Both the variants outperformed epi-1. Specifically for tested Candida spp., var-1 showed two- to four-fold enhancements and var-2 showed two- to eight-fold enhancements compared to epi-1. Electron microscopy confirmed that the mechanism of action involves pore formation thus inducing reactive oxygen species in Candida spp. cell membrane. Computational analysis showed that the peptides have a high tendency to interact with Candida spp. cell membrane proteins such as Exo-β-(1,3)-Glucanase, Secreted Aspartic Proteinase (Sap) 1, and N-terminal Domain Adhesin: Als 9-2, thereby preventing biofilm formation. Conclusions: The in vitro evidence supports the potential use of epi-1 and its variants to be used as an anti-biofilm agent to coat IUDs in the future for therapeutic purposes.

DNA replication origins play a crucial role in cellular division and are evolutionarily conserved across domains. This study investigated the evolutionary transitions of replication origins between archaea and bacteria by analyzing 2733 bacterial and 257 archaeal genomes. Our findings revealed that certain methanogens and bacteria share phylogenetic proximity, suggesting evolutionary interactions across diverse ecological systems. Evolutionary transitions in replication origins may have occurred between gut methanogens and bacteria, haloarchaea (Halogeometricum borinquense DSM 11551 and Halovivax ruber XH-70), halobacteria, and sulfur-reducing archaea. Methanosarcina barkeri (M. barkeri), Methanosaeta thermophila, and Methanococcoides burtonii (M. burtonii) were closely related to respiratory tract bacteria in humans. Methanohalobium evestigatum (M. evestigatum) is strongly linked to the animal gut pathogen Mycoplasma putrefaciens (M. putrefaciens). Several thermophilic hydrogenotrophic methanogens clustered with oral and fish pathogens. Pyrococcus furiosus (P. furiosus) was evolutionarily related to the replication origin of plant pathogens. This study sheds light on the ecological drivers of DNA replication origin evolution and their role in microbial speciation and adaptation. Our findings highlight the influence of mutualistic and parasitic relationships on these evolutionary transitions. It could have significant implications in biotechnology and medicine, such as developing novel antimicrobial strategies and understanding host-pathogen dynamics., (© 2024 Wiley‐VCH GmbH.)

Eutrophication of marine ecosystems is a global problem, particularly in a changing climate and the spreading of Oxygen Minimum Zones (OMZs). The eastern Arabian Sea has both seasonal and permanent/perennial OMZs, but our understanding of the fauna there is extremely poor. So, this study investigated the composition and physiological status (alive or dead) of zooplankton (copepods) in the two OMZs. The study was accomplished by employing a Multiple Plankton Net and vital staining method. Our results showed for the first time that the oxygen deficiency adversely impacted the physiological status of copepods in OMZs which was reflected as (a) a two-fold decrease in zooplankton in the more intense oceanic OMZ compared to the coastal OMZ, (b) a marked increase of dead copepods in both OMZs (50 to 60 %) compared to normoxic waters (35 %) observed in previous studies and (c) higher copepod carcasses (50-70 %) and lower copepods larvae in the intense oceanic OMZ., Competing Interests: Declaration of competing interest The authors declare that there is no conflict of interest with this manuscript., (Copyright © 2024. Published by Elsevier Ltd.)

Rare-earth-based nanomaterials with exceptional electrochemical properties can be obtained via a simple, low-cost, environment-friendly citrate-gel-matrix approach. Owing to their high specific capacitance, strong conductivity, and electrochemical stability, gallium-based materials are capable of withstanding numerous charge-discharge cycles, thus extending the life cycle of a supercapacitor. This tunability enabled researchers to alter the morphology and composition of the electrode to enhance supercapacitor performance. This research describes the investigation of stable neodymium gallium oxides, namely, Nd 3 Ga 5 O 12 and Nd 3 GaO 6 , synthesised via a citrate-gel-matrix method for asymmetric supercapacitor applications. Structural, morphological and optical studies elucidated the outstanding supercapacitor performance of Nd 3 Ga 5 O 12 and Nd 3 GaO 6 . XRD revealed the formation of cubic Nd 3 Ga 5 O 12 and orthorhombic Nd 3 GaO 6 . The vibrational modes, optical properties, surface morphology and oxidation states of Nd 3 Ga x O y phases were examined using Raman spectroscopy, UV-visible spectroscopy, scanning electron microscopy and X-ray photoelectron spectroscopy, respectively. Electrochemical studies using cyclic voltammetry, galvanostatic charge-discharge and electrochemical impedance spectroscopy revealed that the fabricated Nd 3 Ga 5 O 12 electrode resulted in a greater specific capacitance of about 418 F g -1 than Nd 3 GaO 6 , thereby displaying superior electrochemical characteristics. An asymmetric device fabricated with Nd 3 Ga 5 O 12 demonstrated a specific capacitance of about 64 F g -1 , energy density of 20 W h kg -1 and a power density of about 750 W kg -1 . Thus, the favorable attributes of Nd 3 Ga x O y in terms of its electrochemical performance indicate its significant promise for potential application in energy-storage devices.

The cardiovascular sarcoplasmic reticulum (SR) calcium (Ca2+) ATPase is an imperative determinant of cardiac functionality. In addition, anomalies in Ca2+ handling protein and atypical energy metabolism are inherent in heart failure (HF). Moreover, Ca2+ overload in SR leads to mitochondrial matrix Ca2+ overload, which can trigger the generation of Reactive Oxygen Species (ROS), culminating in the triggering of the Permeability Transition Pore (PTP) and Cytochrome C release, resulting in apoptosis that leads to arrhythmias and numerous disorders. Although proteins involved in the molecular mechanism of Ca2+ dysfunction regarding mitochondrial dysfunction remains elusive, this study aims to assess the major Ca2+ handling proteins which may be involved in the Ca2+ malfunction that causes mitochondrial dysfunction and predicting the most effective drug by targeting the analyzed Ca2+ handling proteins through various insilico analyses. Thirteen proteins absorbed from interaction analysis were docked with four optimal phytochemicals from Crataegus oxyacantha (COC). Furthermore, The ADME profile of tyramine, vitexin, Epicatechin, and Epicatechin gallate was acclimated to evaluate potential drugability utilizing QikProp. So, molecular docking evaluations were performed using Glide (Maestro), autodock, and vina. Based on the results of 156 dockings by Maestro, auto-dock, and auto-dock vina, PKAC-a with Epicatechin gallate exhibits good interaction. Therefore, a 2000 ns molecular dynamics (MD) simulation was utilized to assess the feasible phytochemical Epicatechin gallate - PKAC-a complex binding stability utilizing Desmond and this study confirmed that Epicatechin gallate from COC has high possibilities to inhibit the aberrant cardiac Ca2+ signaling proteins due to its conformational rigidity.

Background: Plant-derived phytochemicals are crucial in fighting bacterial infections and in cancer therapy. Objective: This study investigates the phytochemical composition of the ethanolic extract obtained from Caralluma indica ( C. indica ) seeds and assesses its antimicrobial, anticancer, and antioxidant activities. Results: GC-MS analysis found 30 phytochemicals in C. indica seeds, including 5 bioactive compounds that have been shown to have antioxidant, antimicrobial, and cytotoxicity properties, through in silico evaluation. Phytochemical screening of C. indica identified and measured the phenolic compounds, providing insight into its bioactive potential and therapeutic properties. C. indica exhibited robust antioxidant capacity (DPPH, ABTS, nitric oxide, and H 2 O 2 radical scavenging) alongside potent antimicrobial activity against oral pathogen and cytotoxicity activity on a human oral squamous carcinoma cell line (OECM-1) (EC 50 of 169.35 µg/mL) and yeast cell Saccharomyces cerevisiae (215.82 µg/mL), with a selective index of 1.27. The subminimum % MBC/MFC of C. indica significantly reduced biofilm formation against oral pathogens ( p < 0.05). Molecular docking studies showed a strong correlation (r = 0.862) between antifungal and anticancer targets, suggesting that the antimicrobial agents in C. indica contribute to cancer prevention mechanisms. Conclusions: These findings propose C. indica seeds as promising candidates for combating oral pathogens, inhibiting biofilm formation, and reducing the risk of oral cancer progression.

In this study, one-pot multicomponent reactions of novel chromeno[2,3-c]pyrazole derivatives (1-14) were performed using an AlCl 3 catalyst via cyclisation. Various spectral and chromatographic techniques were used to elucidate the structure of the synthesised derivatives (1-14). The synthesised compounds were then inspected for their antibacterial, antioxidant, and tyrosinase inhibition activities. An in silico screening approach was also employed to identify highly potent derivatives. Besides, we utilised density functional theory (DFT) with the B3LYP/6-31G + (d, p) basis set to optimise the newly modified derivatives. This approach was used to calculate various properties, including electron density, electrostatic potential map, interaction strength, frontier molecular orbital energy, and reactivity characteristics. To examine the binding affinity, modes, and stability of the protein-drug complex, molecular docking with the 2Y9X protein structure were employed. The findings from DFT computations, along with physicochemical information and molecular docking binding affinity, showed promising results than standard and low active compound 1. The absorption, metabolism, and cytotoxic characteristics of all the novel derivatives were investigated in the ADMET prediction. Our findings could prove valuable in developing novel drugs for medicinal and pharmaceutical fields., Competing Interests: Declarations. Conflict of interest: The authors report no potential conflicts of interest., (© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

For biomedical applications, material scientists all over the world are working to develop cost-effective technologies and thereby synthesize new nanocomposite materials that are biocompatible, bioactive, scalable and naturally abundant. This study focuses on synthesizing and evaluating nanocomposites of zinc oxide (ZnO) and chitosan (CS) derived from crab shells, in three different weight proportions (1:0.5, 1:1, and 1:2). ZnO/CS nanocomposites were synthesized using a soft-chemical method. Characterization of the nanocomposites was done using XRD, FESEM, EDAX, FTIR, and PL techniques. Among the three formulations, the ZnO/CS nanocomposite with a 1:2 ratio (ZnO/CS) 1:2 exhibited the most significant antioxidant, anti-diabetic, and antibacterial properties. The (ZnO/CS) 1:2 demonstrated 89.46 and 90.85 % of inhibition in DPPH and superoxide free radical scavenging assays, respectively, and showed 91.86 % inhibition against the alpha-glucosidase enzyme. It also exhibited strong antibacterial activity against both gram-positive (Staphylococcus epidermidis, Bacillus subtilis) and gram-negative (Escherichia coli, Pseudomonas aeruginosa) bacteria. Additionally, the cytotoxicity of the (ZnO/CS) 1:2 nanocomposite was assessed against the MCF-7 breast cancer cell line, showing an IC 50 value of 11.58 ± 0.05 μg/mL at 30 μg/mL. The (ZnO/CS) 1:2 nanocomposite shows potential as a candidate for biomedical applications, particularly in antioxidant, anti-diabetic, and antibacterial activities., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Triple-negative breast cancer (TNBC) doesn't have well-defined molecular targets making it unable to treat with chemotherapy also have faster metastatic rate and worse survival rate. In the current study we aim to target TNBC through eco-friendly green synthesized silver nanoparticles having anti-cancer phytoconstituents from the traditional anti-cancer medicinal plant Eclipta alba. Green synthesized silver nanoparticles (AgNPs) are agglomerates of spherical shaped 40-60 nm sized showing characteristic light absorption at 437 nm, banding pattern at 1479, 1285, 1036, and 471 showing and further X-ray diffraction pattern confirm face-centered cubic crystal silver structure exist in the green synthesized silver nanoparticle preparation. Further in vitro anti-oxidant analysis results revealed that green synthesized AgNPs showed 2.6-fold higher anti-oxidant potential (IC50 15.70 g/ml) than that of aqueous plant leaf extract (IC50 39.80 g/ml). In MTT cytotoxicity analysis Eclipta alba plant extract and AgNPs both display dose-dependent cytotoxicity against triple-negative breast cancer cells (MDA-MB-231), although their IC 50 values differ substantially, at 105.80 µg/ml and 77.20 g/ml, respectively. Finally, AgNPs from Eclipta alba tested for anti-leishmanial activity and it showed 91.36 ± 1.05 for promastigotes and 76.62 ± 0.95 for amastigotes at the highest dose of 400 g/ml. Altogether present data showed that Eclipta alba leaf extract actively bonded with silver nanoparticles suppresses the MDA-MB-231 cells growth through high antioxidant characters and anti-leishmanial activity. From together we confirm that Eclipta alba was recommended to a future therapeutic drug and agent to control breast cancer in the clinical level., Competing Interests: Declarations Conflict of interest All authors declare that they have no conflict of interest., (© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

Adaptive network is a powerful presentation to describe different real-world phenomena. However, current models often neglect higher-order interactions (beyond pairwise interactions) and diverse adaptation types (cooperative and competitive) commonly observed in systems such as the human brain and social networks. This work addresses this gap by incorporating these factors into a model that explores their impact on collective properties such as synchronization. Through simplified network representations, we investigate how the simultaneous presence of cooperative and competitive adaptations influences phase transitions. Our findings reveal a transition from first-order to second-order synchronization as the strength of higher-order interactions increases under competitive adaptation. We also demonstrate the possibility of synchronization even without pairwise interactions, provided there is strong enough higher-order coupling. When only competitive adaptations are present, the system exhibits second-order-like phase transitions and clustering. Conversely, with a combination of cooperative and competitive adaptations, the system undergoes a first-order-like phase transition, characterized by a sharp transition to the synchronized state without reverting to an incoherent state during backward transitions. The specific nature of these second-order-like transitions varies depending on the coupling strengths and mean degrees. With our model, we can control not only when the system synchronizes but also the way the system goes to synchronization., (© 2024 Author(s). Published under an exclusive license by AIP Publishing.)

Cells coordinate their activity and regulate biological processes in response to chemical signals. Mimicking natural processes, control over the formation of artificial supramolecular materials is of high interest for their application in biology and medicine. Supramolecular material that can form in response to chemical signals is important for the development of autonomously responsive materials. Herein, a supramolecular hydrogel system is reported enabling in situ generation of hydrogelators in response to a specific chemical signal. Using self-immolative chemistry, spatial control over the formation of supramolecular hydrogel material and structured free-standing hydrogel objects via providing H 2 O 2 locally is demonstrated. In addition, a hybrid system is developed enabling in situ generation of the H 2 O 2 by the action of an enzyme and glucose, providing an extra handle for the development of an intelligent soft material. This generic design should enable the use of various (chemical)stimuli that can be obtained via coupling different stimuli and various chemical and/or biological markers and appears a versatile approach for the design of smart artificial soft materials that can find application in theranostic purposes., (© 2024 Wiley‐VCH GmbH.)

In the present work, the concentrations of toxic heavy metals in mangrove sediments, mangrove leaves, and seeds collected from five stations in the Vattanam mangrove of Palk Bay, southeastern India, as well as the effects of the monsoon season on toxic heavy metals concentrations were determined. The concentrations were 17.5 μg/g for zinc (Zn), 9.11 μg/g for copper (Cu), and 6.03 μg/g for lead (Pb) in sediments; 10.71 μg/g (Zn) and 8.94 μg/g (Cu) in mangrove leaves; and 26.24 μg/g (Zn) and 17.01 μg/g (Cu) in mangrove seeds. At all sampling stations, the Cd concentration was below the limit of detection. These mangroves seem to have adapted successfully to their environment given their selective intake of only essential metals (Cu and Zn) and the restricted movement of non-essential metals (Cd and Pb). The concentrations of metals were below the maximum residual limits regulated by national and international standard limits., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Epinecidin-1 (epi-1), an antimicrobial peptide first identified in marine grouper fish, has multifunctional bioactivities. The present study aims to improve its therapeutic potential via structural modifications that could enhance its antimicrobial activity and stability. To achieve it, we replaced glycine and the first histidine in the parent epi-1 with lysine, which resulted in a peptide with a repeating KXXK motif and improved physiochemical properties related to antimicrobial activity. This modified peptide, referred to as glycine-to-lysine replaced-epi-1, also gained stability and a twofold increase in helical propensity. To produce the active peptide, overlap extension PCR was employed to generate the gene of GK-epi-1 via site-directed mutagenesis, which was then cloned into the pET-32a vector and expressed as a recombinant fusion protein in Escherichia coli C43 (DE3) strain. The recombinant protein was purified and digested with enterokinase to release the active peptide fragment, which was then evaluated for antimicrobial activity and stability. The lysine substitution led to an enhancement in broad-spectrum antimicrobial activity against a wide range of nosocomial pathogenic bacteria., (© 2024 International Union of Biochemistry and Molecular Biology, Inc.)

Interest in red microalgae of the Porphyridium genus has surged due to their richness in phycobiliproteins, polyunsaturated fatty acids, and sulfated polysaccharides. These biomasses and their derivatives find applications across food, feed, nutraceutical, pharmaceutical, and cosmetic industries. A deeper understanding of their properties and extraction methods is essential to optimize downstream processing. This paper comprehensively reviews Porphyridium sp., focusing on cultivation techniques, bioproduct extraction, purification, and characterization. It delves into protein, lipid, and polysaccharide extraction, considering the influence of culture conditions on biomass yield. Various methods like chromatography, electrophoresis, and membrane-based techniques for cell lysis and bioproduct recovery are explored, highlighting their pros and cons. By offering diverse insights, this review aims to inspire innovative research and industry progress in red microalgae biotechnology, contributing to sustainable solutions across sectors., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Our study focused on enhancing the production of anthraquinone derivatives in Oldenlandia umbellata using fungal elicitors. Aspergillus niger, Mucor prayagensis, and Trichoderma viride were used to elicit the anthraquinone derivatives in root cultures. The elicitation process led to an increase in the production of phytochemicals and secondary metabolites, with the highest total protein content observed in A. niger-elicited plants. We performed qualitative and quantitative phytochemical screening of the 80% methanol extract of the plants. Using reverse phase-ultra-fast liquid chromatography, we identified and quantified five anthraquinone compounds: aloe-emodin, rhein, emodin, chrysophanol, and alizarin. The in vitro root samples elicited with A. niger and M. prayagensis exhibited four and three anthraquinone derivatives, respectively, whereas those elicited with T. viride showed only two derivatives. Interestingly, chrysophanol content was the highest in A. niger-elicited root samples. We constructed a system pharmacology framework consisting of 40 nodes and 45 edges with 34 interacting genes. We also identified human proteins that interact with these derivatives, and inferred their roles in cancer-associated pathways. These anthraquinone derivatives interact with various proteins in multiple pathways, including apoptosis, human cytomegalovirus infection, proteoglycans in cancer, MAPK signaling, and hepatitis C, highlighting their potential therapeutic applications in cancer treatment., Competing Interests: Declarations. Ethics approval and consent to participate: The need for ethical approval and individual consent are not required. Competing interests: The authors declare no competing interests., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Green-synthesized silver and copper nanoparticles (NPs), along with their composites, exhibit various biological activities. Ocimum sanctum (Holy basil), traditionally used as medicine in South Asia, treats respiratory disorders, digestive issues, skin diseases and inflammatory conditions. Modern scientific studies support these bioactivities; however, no studies have investigated their bioactivity in combination with NPs. In this study, silver and copper NPs were synthesized using AgNO 3 and CuSO 4 ·5H 2 O solutions, respectively, with Ocimum sanctum leaf extract, and their antibacterial, antioxidant and anticancer properties were examined. Spectroscopic analyses, including Fourier transform infra-red (FTIR), transmission electron microscopy (TEM) and X-ray diffraction (XRD), elucidated the physicochemical characteristics of the green-synthesized nanoparticles ( Os -AgNPs and Os -CuNPs), revealing sizes of 11.7 and 13.1 nm, respectively. The Os -AgNPs: Os -CuNPs nano-composite with a 1:2 ratio exhibited a zone of inhibition ranging from 8 to 12 mm against tested bacterial pathogens. Additionally, the NPs and their composites demonstrated potent antioxidant activity, with notable 2-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity observed in composites with ratios of 2:1 and 1:2. Furthermore, they displayed potential anticancer activity against human leukaemia (Jurkat) cancer cells. Although no distinct difference in anticancer property was observed among the NPs and their composites, our study highlights their well-defined nanostructure and significant biological activity, suggesting their potential as therapeutic agents in the pharmaceutical industry.

Prebiotics are nondigestible components that comprise short-chain carbohydrates, primarily oligosaccharides, which are converted into beneficial compounds by probiotics. Various plant substances with prebiotic properties provide substantial health benefits and are used to prevent different diseases and for medical and clinical applications. Consuming prebiotics gives impeccable benefits since it aids in gut microbial balance. Prebiotic research is primarily concerned with the influence of intestinal disorders. The proposed review will describe recent data on the sources, structures, implementation of prebiotics and potential mechanisms in preventing and treating various disorders, with an emphasis on the gut microbiome. Prebiotics have a distinctive impact on the gastro intestine by explicitly encouraging the growth of probiotic organisms like Bifidobacteria and Lactobacilli. This in turn augments the body's inherent ability to fend off harmful pathogens. Prebiotic carbohydrates may also provide other non-specific advantages due to their fermentation in the large intestine. Additional in vivo research is needed to fully comprehend the interactions between prebiotics and probiotics ingested by hosts to improve their nutritional and therapeutic benefits., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

This article studies a non-Hermitian Su-Schrieffer-Heeger model which has periodically staggered Hermitian and non-Hermitian dimers. The changes in topological phases of the considered chiral symmetric model with respect to the introduced non-Hermiticity are studied where we find that the system supports only complex eigenspectra for all values of u  ≠ 0 and it stabilizes only non-trivial insulating phase for higher loss-gain strength. Even if the system acts as a trivial insulator in the Hermitian limit, the increase in loss-gain strength induces phase transition to non-trivial insulating phase through a (gapless) semi-metallic phase. Interesting phenomenon is observed in the case where Hermitian system acts as a non-trivial insulator. In such a situation, the introduced non-Hermiticity neither leaves the non-trivial phase undisturbed nor induces switching to trivial phase. Rather, it shows transition from non-trivial insulating phase to the same where it is mediated by the stabilization of (non-trivial) semi-metallic phase. This unusual transition between the non-trivial insulating phases through non-trivial semi-metallic phase gives rise to a question regarding the topological states of the system under open boundary conditions. So, we analyze the possibility of stable edge states in these two non-trivial insulating phases and check the characteristic difference between them. In addition, we study the nature of topological states in the case of non-trivial gapless (semi-metallic) region., (© 2024 IOP Publishing Ltd. All rights, including for text and data mining, AI training, and similar technologies, are reserved.)

In cellular environments, certain synthetic molecules can form nanostructures via self-assembly, impacting molecular imaging, and biomedical applications. Control over the formation of these self-assembled nanostructures in subcellular organelle is challenging. By the action of stimuli, either present in the cellular environment or applied externally, in situ generation of molecular precursors can lead to accumulation and supramolecular nanostructure formation, resulting in efficient bioimaging. Here, we summarize smart fluorophore-based ordered nanostructure preparation at specific organelles for efficient bioimaging and therapeutic application towards cancer theranostics. We also present challenges and an outlook regarding intercellular self-assembly for theranostics application. Altogether, smart nanostructured materials with fluorescence read-outs at specific subcellular compartments would be beneficial in synthetic biology and precision therapeutics., Competing Interests: Declarations. Conflicts of Interest: There are no conflicts to declare. Ethics Approval and Consent to Participate: There are no human and animal subjects in this review and informed consent is not applicable., (© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

Model systems are critical in biomedical and preclinical research. Animal and in vitro models serve an important role in our current understanding of human physiology, disease pathophysiology, and therapy development. However, if the system is between cell culture and animal models, it may be able to overcome the knowledge gap that exists in the current system. Studies employing ex vivo organs as models have not been thoroughly investigated. Though the integration of other organs and systems has an impact on many biological mechanisms and disorders, it can add a new dimension to modeling and aid in the identification of new possible therapeutic targets. Here, we have discussed why the ex vivo organ model is desirable and the importance of the inclusion of organs from diverse species, described its historical aspects, studied organs as models in scientific research, and its ex vivo stability. We also discussed, how an ex vivo organ model might help researchers better understand organ physiology, as well as organ-specific diseases and therapeutic targets. We emphasized how this ex vivo organ dynamics will be more competent than existing models, as well as what tissues or organs would have potentially viable longevity for ex vivo modeling including human tissues, organs, and/or at least biopsies and its possible advantage in clinical medicine including organ transplantation procedure and precision medicine., Competing Interests: Declarations. Conflict of interest: The authors have no conflict of interest in any part of this manuscript. Ethical approval: Not applicable., (© 2024. The Author(s), under exclusive licence to the Japanese Society for Artificial Organs.)

In this study, we employed DFT calculations to elucidate the mechanism of NO generation from nitrite via PPh 3 -induced oxygen atom transfer (OAT) in the unsymmetrical β-diketiminato copper(II), LCu II ONO. We discovered that the OAT process involves the cooperation of two PPh 3 ligands and follows the mechanism distinct from that of the symmetrical β-diketiminato analogue. The Δ G ‡ value, calculated to be 34.8 kcal mol -1 , closely matches experimental data. The finding is further supported by analyzing the OAT product yields with varying equivalents of PPh 3 . The penta-coordinated species 5a, with PPh 3 occupying the axial site, forms in the final stage of the OAT process. The isomerization of 5a and the decoordination of the hemilabile pyridyl arm synergistically reduce Cu(II) to Cu(I), facilitating NO release from the Cu(I) centre. These computational results provide valuable insights for the ligand design for PPh 3 -induced OAT reactions to produce NO in Cu(II) nitrite systems.

Atherosclerosis (ASC) is characterized by foam cell-mediated plaque formation, vascular endothelial inflammation, and lipidosis and is the rudimentary cause of cardiovascular diseases. This is the pre-eminent global factor of mortality. This etiological paradigm is significantly influenced by several proteins, where 23 pivotal proteins involved in ASC were meticulously gleaned on the basis of literature studies. The crux of the present study was aimed to probe the drugability of four active phytochemicals from Crataegus oxyacantha (COC): epicatechin, gallate, tyramine, and vitexin against the selected 23 proteins. The molecular docking analysis was judiciously administered via Glide, the binding free energy was calculated in detail utilizing the prime molecular mechanics-generalized Born surface area (MM-GBSA) module, and a deeper comprehensive investigation of protein-ligand dynamic associations was elucidated through Desmond. Drawing from the upper echelons of our docking results, the molecular dynamics simulation outcomes revealed that the macrophage migration inhibitory factor and prethrombin-1 showed persistent binding nature with gallate. The bioactive compound known as gallate sourced from COC shows the best molecular association with pivotal proteins involved in ASC and has a promising therapeutic potential for drug development endeavors., Competing Interests: The authors declare no competing financial interest., (© 2024 The Authors. Published by American Chemical Society.)

Triclocarban (TCC) and triclosan (TCS) are antibacterial compounds used in household, veterinary, industrial and personal care products, which are known to be environmental pollutants and also toxic to organisms. The toxicological effects of these antibacterial chemicals on higher organisms have been studied in detail. But in lower invertebrates like hydra, it is still rare and yet to be explored. In this study, the toxicological effects of these two antibacterial compounds in Hydra vulgaris was performed to clearly understand the organismal, developmental, molecular and behavioural changes. Both TCC and TCS are toxic with respective LC 50 values of 0.09 and 0.25 mg/L, whereas TCC is comparatively more toxic than TCS. The structural damage of battery cell complexes (BCCs) on the tentacles was observed and ultimately made prey capturing difficult. It was evident that TCC and TCS exposure caused developmental toxicity by affecting reproduction and regeneration in H. vulgaris at higher sublethal doses (0.045 and 0.125 mg/L, respectively). TCC and TCS also caused DNA damage resulting in apoptosis. This study further reveals that these two antibacterial compounds are teratogenic and genotoxic in the organisms., (© 2024 John Wiley & Sons Ltd.)

Danger-associated molecular patterns (DAMPs) are released on the onset of tissue injury or death, which tend to trigger innate immunity and regulate various immune pathways. Among the various DAMP molecules, S100A8 and S100A9 belonging to Ca 2+ binding proteins with EF-hands and Zn 2+ ion binding sites have been implicated in aggravating the pathogenesis of rheumatoid arthritis (RA), upon interaction with pattern recognition receptors (PRR) such as TLR4, RAGE and CD36 receptors. Thus, the present study aims to assess the effect of Ca 2+ or Zn 2+ ions on the interaction of S100A8 and S100A9 proteins towards the PRRs. Protein-protein interaction analysis showed that the TLR4-S100A8Ca 2+ Zn 2+ , TLR4-S100A8 Zn 2+ , RAGE-S100A8/A8Zn 2+ , RAGE-S100A8/A8Ca 2+ , CD36-S100A8Ca 2+ , and CD36-S100A9/A9Ca 2+ showed higher affinity against each other. The 100 ns molecular dynamics simulation showed that the TLR4-S100A8Ca 2+ , RAGE-S100A8/A8Ca 2+ and CD36-S100A8Ca 2+ complexes showed minimal fluctuations in their trajectory indicating that Ca 2+ bound complexes were more stable than the other complexes. Furthermore, SPR analysis showed that S100A9 exhibited higher binding affinity towards PRRs in the presence of Ca 2+ and Zn 2+ ions. Considering the fact that physiological levels of both Ca 2+ and Zn 2+ ions play a critical role in the binding of S100A8 and S100A9 proteins against the PRRs, it can be emphasized that the S100A9 and RAGE receptors could be the critical players in the RA pathogenesis due to its impeccable binding towards the PRRs in the presence of both Ca 2+ and Zn 2+ ions. Nonetheless, further in vivo, and in vitro studies are imperative to validate these findings and identify potential targets for RA treatment., Competing Interests: Compliance with ethical standards. Conflict of interest: The authors declare no competing interests., (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

This study explores spatiotemporal variations of microplastics (MPs) in beach sediments along India's southeast coast, focusing on Tamil Nadu and Puducherry from 2020 to 2021. The MPs were extracted from the sediments through density separation and wet peroxidation. Following extraction, they were quantified and physically characterized using stereo-microscopy and chemically analyzed using ATR-FTIR. During the monsoon, Chennai (923 ± 380 MPs/kg) exhibited the highest MP abundance, followed by Puducherry (805 ± 222 MPs/kg), Nagapattinam (799 ± 257 MPs/kg), Thoothukudi (653 ± 258 MPs/kg), Rameswaram (585 ± 151 MPs/kg), and Kanyakumari (344 ± 71 MPs/kg). Similarly, in summer, Chennai (719 ± 192 MPs/kg) recorded the highest mean, trailed by Puducherry (645 ± 163 MPs/kg), Rameswaram (529 ± 138 MPs/kg), Nagapattinam (523 ± 95 MPs/kg), Thoothukudi (492 ± 104 MPs/kg), and Kanyakumari (335 ± 72 MPs/kg). Fibers predominated as the most common MP type. FTIR revealed polymers like polystyrene, polyethylene terephthalate, polyethylene, polypropylene, polyurethane, and polyamide. The Polymer Hazard Index indicated high polymer pollution risk, while the Pollution Load Index showed minimal contamination. The Potential Ecological Risk Index revealed low-to-medium MP pollution levels. Tailored strategies addressing plastic usage reduction and mitigation of terrestrial MP sources are imperative for coastal ecosystem resilience., Competing Interests: Declarations. Ethics approval: This paper does not need any ethical approval. Consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

External ocular infections are of serious global concern, which cause significant visual morbidity and even blindness, particularly in low-income and resource-limited countries. Herewith, we are reporting the profile of bacteria and fungi causing external ocular infections and the associated factors in Arba Minch, southern Ethiopia. An institution-based cross-sectional study was conducted among 259 suspected individuals with external ocular infections from 01 January to 10 June 2020 in Arba Minch General Hospital. The demographic data were obtained using a structured questionnaire, while corneal scrapings and conjunctival swabs were collected for culture. Samples were inoculated onto MacConkey, blood, mannitol salt and Sabouraud dextrose agar plates. Bacteria were characterised using conventional microbiological techniques. Fungal isolates were identified by culture and morphology by means of microscopy. Antibiotic susceptibility tests for bacteria were performed via the Kirby-Bauer disk diffusion technique. A logistic regression analysis determined the association between dependent and independent variables; P values ≤ 0.05 were considered statistically significant. The most common clinical manifestations in culture-proven cases were conjunctivitis (38.6%, n = 100) and blepharoconjunctivitis (25.9%, n = 67). The overall prevalence of external ocular bacterial and fungal infections was 73.2 (n = 115) and 26.8% (n = 42), respectively. Isolates of Staphylococcus aureus (24.8%, n = 39) and Pseudomonas aeruginosa (13.4%, n = 21) were the predominant Gram-positive and Gram-negative bacteria, respectively. The prevalence of multidrug-resistant bacteria was 71.3% (n = 82). The World Health Organisation-prioritised bacteria such as methicillin-resistant S. aureus (61.5%, n = 24), extended-spectrum beta-lactamase (22.6%, n = 12), and carbapenemase-producing Enterobacteriaceae (15.1%, n = 8) were also detected. The prominent fungi recovered from patients with keratitis and conjunctivitis were Aspergillus spp. and Candida albicans, respectively (9.5%, n = 15 each). Age (26-49 years) and family income were statistically associated with ocular infections (P ≤ 0.05). The results provide insights into the characteristics of major ocular bacterial and fungal pathogens circulating in the ophthalmic patients of Arba Minch. Conjunctivitis is the main ocular manifestation observed, with a predominance of S. aureus. More than two-thirds of the ocular bacteria were MDR, and the highest prevalence corresponds to S. aureus. The aggravation of multi-drug resistance, including the WHO-prioritised ones, warrants periodic evaluations., Competing Interests: Declarations. Competing interests: The authors declare no competing interests., (© 2024. The Author(s).)

Recent studies highlight the role of brain metabolites in regulation of neuronal signals and behaviour. To understand the underlying mechanism, brain metabolites and associated signaling molecules were examined in early adolescent rat experienced CRS. Rats were tested for their learning and memory ability, and their metabolite profile was evaluated using Gas chromatography-mass spectrometry (GC-MS). Differences in metabolites were examined by variable importance in projection (VIP) and multivariate analysis. Ingenuity Pathway Analysis (IPA) and KEGG ID were performed for the identified metabolites. We found that CRS altered the metabolites that were involved in biosynthesis of steroid hormone, aminoacyl t-RNA, L-Dopa biosynthesis, and metabolism of tyrosine, fatty acid, and purine. Further analysis showed reduction of 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR, a metabolite involved in purine metabolism) an AMP kinase activator, influenced the hypoxanthine-guanine phosphoribosyltransferase (HPRT), serotonin transporter (SERT), postsynaptic density protein (PSD) -95, its phosphorylation and brain-derived neurotrophic factor (BDNF) in CRS animals, which displayed deficit in memory. The AICAR treated CRS rats showed improved memory and altered metabolites and other molecules (HPRT, SERT, PSD-95 and BDNF) levels were restored. Our analysis revealed that CRS induced changes in metabolites possibly altered synaptic plasticity and memory in which HPRT, SERT-PSD95-BDNF associated pathway involved. Taken together, our observation provides initial insight into how stress differently influences the metabolic pathway, and associated behaviour. Further study will help to develop pharmacological intervention strategies., Competing Interests: Declarations Ethical Approval The experimental protocol reviewed and approved by Institutional Animal Ethics Committee (Ref. No.: BDU/IAEC/P28/2024), assuring that the number of animals used was minimum. Competing Interests The authors declare no competing interests., (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

The marine ecosystem contains an assorted range of organisms, among which macroalgae stands out marine resources as an invaluable reservoir of structurally diverse bioactive compounds. Marine macroalgae are considered as primary consumers have gained more attention for their bioactive components. Sulfated polysaccharides (SPs) are complex polymers found in macroalgae that play a crucial role in their cell wall composition. This review consolidates high-tech methodologies employed in the extraction of macroalgal SPs, offering a valuable resource for researchers focuses in the pharmacological relevance of marine macromolecules. The pharmacological activities of SPs, focusing on their therapeutic action by encompassing diverse study models are summarized. Furthermore, in silico docking studies facilitates a comprehensive understanding of SPs interactions with their binding sites providing a valuable insight for future endeavors. The biological properties of algal SPs, along with a brief reference to mode of action based on different targets are presented. This review utilizes up-to-date research discoveries across various study models to elucidate the biological functions of SPs, focusing on their molecular-level mechanisms and offering insights for prospective investigations. Besides, the significance of SPs from seaweeds is highlighted, showcasing their potential beneficial applications in promoting human health. With promising biomedical prospects, this review explores the extensive uses and experimental evidence supporting the important roles of SPs in various fields., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

The Batagur kachuga (B. kachuga), commonly known as the Red-crowned roofed turtle, is a critically endangered species native to India and its neighboring countries like Bangladesh, and Nepal. The present study is the first report of the complete mitochondrial genome of B. kachuga (16,517 bp) construed via the next-generation sequencing (NGS) approach from eggshell DNA. There are 22 transfer RNAs (tRNAs), 2 ribosomal RNAs (rRNAs), 13 protein-coding genes (PCGs), and one putative control region (CR/D-loop) in the mitogenome. The CR region from the current study reveals conserved TAS, CD, and CSB domains and two AT-rich tandem repeat regions. Most genes are encoded in the heavy strand except the NADH dehydrogenase subunit 6 (ND6) gene and seven tRNA genes. Most PCGs start with the initiation codon ATG, except the COI (Cytochrome Oxidase Subunit-I) gene, which starts with the GTG codon. The present investigation also predicts the distinctive cloverleaf structures of tRNAs except for tRNA-Ser1 and tRNA-Ser-2, which lack a DHU arm. The comparative analysis of Ka/Ks with other 33 species from Order Testudines, in relation to B. kachuga, revealed negative selection in most PCGs, indicating a process of preservation and purification that aids in eliminating undesirable or detrimental substitutes. Phylogenetic analysis of this species has been analysed using the complete mitogenome of 33 turtle species. The maximum likelihood phylogenetic tree strongly supports each family in different clades and also reveals a close relationship between the Pangashura and Batagur genera. Our study suggests the generation of genome-wide molecular data, in terms of mitogenomes, SNPs, and SSRs, is needed to improve the understanding of this species and their phylogenetics and evolutionary relationships, which will help to improve the conservation efforts of this species., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Heavy metals emanate from diverse anthropogenic activities and the top soil in the vicinity of these activities acts as an immediate sink and facilitates diffusion of heavy metals into the food chain. In the semi-arid plains of India, Prosopis juliflora is the most common and dominant weed along the motorways and barren lands including industrial environs. This investigation hypothesizes the adaptive nature of Prosopis juliflora in the metal enriched soils and attempts to understand its hyper-accumulating potential of metals besides bioconversion/detoxification capability. Prosopis juliflora samples (root, stem, leaves, and pods) from 100 sites in the environs of anthropogenic activities (vehicular emissions and industrial operations) were analyzed for heavy metal concentrations (Cu, Fe, Cr, Cd, Ni, Pb). Prosopis juliflora accumulate metals at the rate of 0.138 mg/kg/day DW for Copper (Cu), Fe: 0.142 mg/kg/day DW, Cr: 0.114 mg/kg/day DW, Ni: 0.048 mg/kg/day DW, Pb: 0.052 mg/kg/day DW, Cd: 0.009 mg/kg/day DW. Furthermore, X-ray Photoelectron Spectroscopy (XPS) metal oxidation state analysis revealed that in the pods of Prosopis juliflora heavy metals (Fe, Cr, Pb) largely existed in non-toxic form (toxic:non-toxic - 3:6), while in the under canopy soil, metals predominantly existed in toxic form (toxic:non-toxic - 7:2); conclusively XPS results ascertains the heavy metal bioconversion/detoxification potential of the plant. These findings suggest that presence of Prosopis juliflora coppice in the barren landscapes across the transportation corridors and metal based industrial zones may ideally favor phyto-remediation of heavy metals., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Background: Green synthesized nanoparticles (NPs) have gained increasing popularity in recent times due to their broad spectrum of antimicrobial properties. This study aimed to develop a phytofabrication approach for producing cuprous (Cu 2 O) and cupric oxide (CuO) NPs using a simple, non-hazardous process and to examine their antimicrobial properties. Methods: The synthesis employed Bidens pilosa plant extract as a natural reducing and stabilizing agent, alongside copper chloride dihydrate as the precursor. The biosynthesized NPs were characterized through various techniques, including X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier-transform infrared (FT-IR) spectroscopy, ultraviolet-visible (UV-Vis) spectroscopy, scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDS). Results: XRD analysis confirmed that the synthesized CuO and Cu 2 O NPs exhibited a high degree of crystallinity, with crystal structures corresponding to monoclinic and face-centered cubic systems. SEM images revealed that the NPs displayed distinct spherical and sponge-like morphologies. EDS analysis further validated the purity of the synthesized CuO NPs. The antimicrobial activity of the CuO and Cu 2 O NPs was tested against various pathogenic bacterial strains, including Staphylococcus aureus , Pseudomonas aeruginosa , Escherichia coli , and Bacillus cereus , with the minimum inhibitory concentration (MIC) used to gauge their effectiveness. Conclusions: The results showed that the phytosynthesized NPs had promising antibacterial properties, particularly the Cu 2 O NPs, which, with a larger crystal size of 68.19 nm, demonstrated significant inhibitory effects across all tested bacterial species. These findings suggest the potential of CuO and Cu 2 O NPs as effective antimicrobial agents produced via green synthesis.