18 results on '"Banfi, Stefano"'
Search Results
2. A tetracationic porphyrin with dual anti-prion activity.
- Author
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Masone A, Zucchelli C, Caruso E, Lavigna G, Eraña H, Giachin G, Tapella L, Comerio L, Restelli E, Raimondi I, Elezgarai SR, De Leo F, Quilici G, Taiarol L, Oldrati M, Lorenzo NL, García-Martínez S, Cagnotto A, Lucchetti J, Gobbi M, Vanni I, Nonno R, Di Bari MA, Tully MD, Cecatiello V, Ciossani G, Pasqualato S, Van Anken E, Salmona M, Castilla J, Requena JR, Banfi S, Musco G, and Chiesa R
- Abstract
Prions are deadly infectious agents made of PrP
Sc , a misfolded variant of the cellular prion protein (PrPC ) which self-propagates by inducing misfolding of native PrPC . PrPSc can adopt different pathogenic conformations (prion strains), which can be resistant to potential drugs, or acquire drug resistance, hampering the development of effective therapies. We identified Zn(II)-BnPyP, a tetracationic porphyrin that binds to distinct domains of native PrPC , eliciting a dual anti-prion effect. Zn(II)-BnPyP binding to a C-terminal pocket destabilizes the native PrPC fold, hindering conversion to PrPSc ; Zn(II)-BnPyP binding to the flexible N-terminal tail disrupts N- to C-terminal interactions, triggering PrPC endocytosis and lysosomal degradation, thus reducing the substrate for PrPSc generation. Zn(II)-BnPyP inhibits propagation of different prion strains in vitro , in neuronal cells and organotypic brain cultures. These results identify a PrPC -targeting compound with an unprecedented dual mechanism of action which might be exploited to achieve anti-prion effects without engendering drug resistance., Competing Interests: J.C. and H.E., as part of the company ATLAS Molecular Pharma S.L., declare that they have no conflicts of interest, as the company had no role in study design or funding, nor will they, or their immediate family members, benefit financially from the findings reported. All the other authors declare no competing interests., (© 2023 The Author(s).)- Published
- 2023
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3. Improving chestnut physicochemical properties through fermentation - Development of chestnut Amazake.
- Author
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Santos MV, Banfi S, Santos R, Mota M, Raymundo A, and Prista C
- Abstract
The increased awareness of population regarding the impact of consumption habits is leading to interest in new, innovative, diversified and health promoting foods. In this work, two new amazake fermented products were developed with chestnut ( Castanea sativa Mill.), using rice or chestnut koji as source of glycolytic enzymes. The analysis of the amazakes evolution showed improvements in chestnuts physicochemical characteristics. The fermented products presented higher values of soluble protein, sugars, starches, antioxidant capacity, and similar values of ascorbic acid for chestnut koji amazake. The adhesiveness increased, which is related to the enhanced concentrations of sugars and starches. The evolution into less structured products was observed in the firmness followed by a consistent decrease of the viscoelastic moduli. The developed chestnut amazakes can represent a suitable alternative to traditional amazake, creating an opportunity for valorisation of chestnut industrial by-products, as new, tasty, and nutritive fermented products with potential functional characteristics., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2023 The Authors.)
- Published
- 2023
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4. Drug delivery systems for the photodynamic application of two photosensitizers belonging to the porphyrin family.
- Author
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Malacarne MC, Banfi S, Rugiero M, and Caruso E
- Abstract
Photodynamic therapy involves the concomitant action of three components, light with an appropriate wavelength, molecular oxygen, and a molecule, able to absorb an electromagnetic radiation, called photosensitizer (PS). A fundamental aspect is the bioavailability of the PS that is directly related to some physicochemical properties of the PS itself as it should feature a certain degree of lipophilicity to easily cross the cell membrane, however, at the same time, should be sufficiently water-soluble to navigate in the bloodstream. Consequently, the use of a system for drug delivery becomes essential when photosensitizers with a high degree of lipophilicity are considered. In this work, we present three different drug delivery systems, microemulsions, emulsions and liposomes all capable of carrying a PS belonging to the porphyrin family: the tetraphenyl porphyrin (TPP) and the 4-hydroxyphenyl porphyrin (THPP), which show a relevant different degree of lipophilicity. A series of microemulsions (ME) and emulsions (E) were prepared, among which two formulations, one for THPP and one for TPP, have been chosen. The stability of these two carriers was monitored over time and under various temperature conditions. With the same criteria, two liposomal formulations have been also identified and analyzed. The four formulations mentioned above (one ME, one E and two liposomes) have been tested on SKOV3 tumor cell line comparing the photodynamic activity of the porphyrin formulations versus the aqueous/organic (DMSO) solution of the same two PSs. The results show that all the formulations have proved to be excellent carriers and that the liposomal formulation enhance the photodynamic efficacy of both porphyrins., (© 2021. The Author(s).)
- Published
- 2021
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5. New BODIPYs for photodynamic therapy (PDT): Synthesis and activity on human cancer cell lines.
- Author
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Caruso E, Malacarne MC, Marras E, Papa E, Bertato L, Banfi S, and Gariboldi MB
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- Apoptosis drug effects, Boron Compounds chemical synthesis, Boron Compounds metabolism, Boron Compounds pharmacology, Cell Cycle Checkpoints drug effects, Cell Line, Tumor, Cell Movement drug effects, Cell Survival drug effects, Drug Stability, Humans, Light, Neoplasms drug therapy, Neoplasms pathology, Photochemotherapy, Photosensitizing Agents chemistry, Photosensitizing Agents metabolism, Photosensitizing Agents pharmacology, Reactive Oxygen Species metabolism, Singlet Oxygen metabolism, Boron Compounds chemistry, Photosensitizing Agents chemical synthesis
- Abstract
A new class of compounds based on the 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene core, known as BODIPYs, has attracted significant attention as photosensitizers suitable for application in photodynamic therapy (PDT), which is a minimally invasive procedure to treat cancer. In PDT the combination of a photosensitizer (PS), light, and oxygen leads to a series of photochemical reactions generating reactive oxygen species (ROS) exerting cytotoxic action on tumor cells. Here we present the synthesis and the study of the in vitro photodynamic effects of two BODIPYs which differ in the structure of the substituent placed on the meso (or 8) position of the dipyrrolylmethenic nucleus. The two compounds were tested on three human cancer cell lines of different origin and degree of malignancy. Our results indicate that the BODIPYs are very effective in reducing the growth/viability of HCT116, SKOV3 and MCF7 cells when irradiated with a green LED source, whereas they are practically devoid of activity in the dark. Phototoxicity occurs mainly through apoptotic cell death, however necrotic cell death also seems to play a role. Furthermore, singlet oxygen generation and induction of the increase of reactive oxygen species also appear to be involved in the photodynamic effect of the BODIPYs. Finally, it is worth noting that the two BODIPYs are also able to exert anti-migratory activity., (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Published
- 2020
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6. In vitro photodynamic treatment of cancer cells induced by aza-BODIPYs.
- Author
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Malacarne MC, Banfi S, and Caruso E
- Subjects
- Antineoplastic Agents chemical synthesis, Antineoplastic Agents chemistry, Apoptosis drug effects, Boron Compounds chemical synthesis, Boron Compounds chemistry, Cell Line, Tumor, Cell Proliferation drug effects, Drug Screening Assays, Antitumor, Humans, Molecular Structure, Neoplasms pathology, Photosensitizing Agents chemical synthesis, Photosensitizing Agents chemistry, Antineoplastic Agents pharmacology, Boron Compounds pharmacology, Neoplasms drug therapy, Photochemotherapy, Photosensitizing Agents pharmacology
- Abstract
Two aza-BODIPY photosensitizes (PSs, compounds 7 and 8), featuring an iodine atom on each pyrrolic unit of their structure, were synthesized in fairly good yields starting from commercial products and tested in vitro on two human cancer cell lines (HCT116 and SKOV3) to assess their photodynamic efficacy. After treating the cell cultures with variable concentrations of 7 or 8 and incubating for the desired incubation time, the cells were irradiated for two hours with a red-light emitting diode (LED) device; afterwards the extent of cell death was determined by MTT assay. Besides the killing effect, the new PSs were also studied to determine further parameters related to photodynamic efficacy, such as the resistance towards photobleaching, the rate of singlet oxygen production, the fluorescence quantum yields, the cellular uptake and the localization inside the cells and, finally, flow cytometric analysis for apoptosis. Considering the results as a whole, these aza-BODIPYs can be considered to be promising photosensitizers because of their IC
50 values being below micromolar concentrations and for more rather interesting features. Actually, these molecules have proved to be: (a) quite stable towards photobleaching; (b) good producers of singlet oxygen and (c) highly penetrating the cells with a wide distribution in the cytosol. Furthermore, in accordance with the good rate of singlet oxygen production, the apoptotic cells reach 30% and this allows us to assume a low inflammatory effect of the in vivo PDT treatment; thus a possible in vivo application of these aza-BODIPYs might be plausible.- Published
- 2020
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7. Cationic diarylporphyrins: In vitro versatile anticancer and antibacterial photosensitizers.
- Author
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Caruso E, Malacarne MC, Banfi S, Gariboldi MB, and Orlandi VT
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- Anti-Bacterial Agents chemical synthesis, Anti-Bacterial Agents pharmacology, Antineoplastic Agents chemical synthesis, Antineoplastic Agents pharmacology, Cations chemistry, Cell Line, Tumor, Cell Proliferation drug effects, Cell Proliferation radiation effects, Gram-Negative Bacteria drug effects, Gram-Positive Bacteria drug effects, Humans, Light, Photosensitizing Agents chemical synthesis, Photosensitizing Agents pharmacology, Porphyrins chemical synthesis, Porphyrins pharmacology, Reactive Oxygen Species metabolism, Anti-Bacterial Agents chemistry, Antineoplastic Agents chemistry, Photosensitizing Agents chemistry, Porphyrins chemistry
- Abstract
The visible light combined with photosensitizers (PSs) is exploited in both antitumoral and antimicrobial fields inducing a photo-oxidative stress within the target cells. Among the different PSs, porphyrins belong to the family of the most promising compounds to be used in clinical photodynamic applications. Although in the last years many porphyrins have been synthesised and tested, only a few reports concern the in vitro effects of the 5,15-diarylporphyrins. In this work, the activity of four 5,15-diarylporphyrins (compounds 7-10), bearing alkoxy-linked pyridinium appendixes, have been tested on cancer cell lines and against bacterial cultures. Among the synthetized PSs, compounds 7 and 9 are not symmetrically substituted porphyrins showing one cationic charge tethered at the end of one 4C or 8C carbon chains, respectively. On the other hand, compounds 8 and 10 are symmetrically substituted and show two chains of C4 and C8 carbons featuring a cationic charge at the end of both chains. The dicationic 8 and 10 were more hydrophilic than monocationic 7 and 9, outlining that the presence of two pyridinium salts have a higher impact on the solubility in the aqueous phase than the lipophilic effect exerted by the length of the alkyl chains. Furthermore, these four PSs showed a similar rate of photobleaching, irrespective of the length and number of chains and the number of positive charges. Among the eukaryotic cell lines, the SKOV3 cells were particularly sensitive to the photodynamic activity of all the tested diarylporphyrins, while the HCT116 cells were found more sensitive to PSs bearing C4 chain (7 and 8), regardless the number of cationic charges. The photo-induced killing effect of these porphyrins was also tested against two different bacterial cultures. As expected, the Gram positive Bacillus subtilis was more sensitive than the Gram negative Escherichia coli, and the dicationic porphyrin 8, bearing two C4 chains, was the most efficient on both microorganisms. In conclusion, the new compound 8 seems to be an optimal candidate to deepen as versatile anticancer and antibacterial photosensitizer., (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Published
- 2019
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8. Core-shell poly-methyl methacrylate nanoparticles covalently functionalized with a non-symmetric porphyrin for anticancer photodynamic therapy.
- Author
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Ballestri M, Caruso E, Guerrini A, Ferroni C, Banfi S, Gariboldi M, Monti E, Sotgiu G, and Varchi G
- Subjects
- Antineoplastic Agents chemistry, Antineoplastic Agents pharmacology, Antineoplastic Agents therapeutic use, Apoptosis drug effects, Cell Line, Tumor, Colonic Neoplasms drug therapy, Colonic Neoplasms pathology, HCT116 Cells, Humans, Microscopy, Electron, Scanning, Particle Size, Photochemotherapy, Photosensitizing Agents pharmacology, Photosensitizing Agents therapeutic use, Porphyrins pharmacology, Porphyrins therapeutic use, Nanoparticles chemistry, Photosensitizing Agents chemistry, Polymethyl Methacrylate chemistry, Porphyrins chemistry
- Abstract
Photodynamic therapy (PDT) is an anticancer modality that exploits singlet oxygen and other reactive oxygen species, that are formed by selective irradiation of photosensitive molecules, to kill cancer cells. Most photosensitizers (PS) are hydrophobic and poorly soluble in water and several nanoplatforms have been established to achieve a more efficient delivery. Moreover, the covalent binding of the PS to nanoparticles could in principle reduce unwanted bleaching of the PS, while preserving its photodynamic activity. In this study we report the synthesis of a novel non-symmetrical diaryl-porphyrin suitably modified with a polymerizable pendant, that was used for the preparation of core-shell poly-methyl methacrylate nanoparticles covalently loaded with the diaryl-porphyrin (PMMA@PorVa). Particles, which were prepared with two different porphyrin loadings, are spherical in shape and with a narrow hydrodynamic diameter around 70 nm and a positive zeta potential. Their photo-toxicity was tested against the human colon carcinoma cell line HCT116 and the human ovarian adenocarcinoma cell line SKOV3. PMMA@PorVa were able to inhibit tumor cells proliferation similarly to the free porphyrin, thus confirming that the covalent attachment of the PS to PMMA nanoparticles allows to preserve PS photodynamic activity and in vitro efficacy. Flow cytometric analysis of apoptotic cells demonstrates that, especially in SKOV3 cells, the free diaryl-porphyrin is more effective in inducing apoptosis., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2018
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9. Synthesis, photodynamic activity, and quantitative structure-activity relationship modelling of a series of BODIPYs.
- Author
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Caruso E, Gariboldi M, Sangion A, Gramatica P, and Banfi S
- Subjects
- Apoptosis drug effects, Boron Compounds chemical synthesis, Boron Compounds chemistry, Cell Line, Tumor, Chromatography, High Pressure Liquid, Humans, Quantitative Structure-Activity Relationship, Spectrometry, Mass, Electrospray Ionization, Spectrum Analysis methods, Boron Compounds therapeutic use, Photochemotherapy
- Abstract
Here we report the synthesis of eleven new BODIPYs (14-24) characterized by the presence of an aromatic ring on the 8 (meso) position and of iodine atoms on the pyrrolic 2,6 positions. These molecules, together with twelve BODIPYs already reported by us (1-12), represent a large panel of BODIPYs showing different atoms or groups as substituent of the aromatic moiety. Two physico-chemical features (
1 O2 generation rate and lipophilicity), which can play a fundamental role in the outcome as photosensitizers, have been studied. The in vitro photo-induced cell-killing efficacy of 23 PSs was studied on the SKOV3 cell line treating the cells for 24h in the dark then irradiating for 2h with a green LED device (fluence 25.2J/cm2 ). The cell-killing efficacy was assessed with the MTT test and compared with that one of meso un-substituted compound (13). In order to understand the possible effect of the substituents, a predictive quantitative structure-activity relationship (QSAR) regression model, based on theoretical holistic molecular descriptors, was developed. The results clearly indicate that the presence of an aromatic ring is fundamental for an excellent photodynamic response, whereas the electronic effects and the position of the substituents on the aromatic ring do not influence the photodynamic efficacy., (Copyright © 2017 Elsevier B.V. All rights reserved.)- Published
- 2017
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10. Antimicrobial and anti-biofilm effect of a novel BODIPY photosensitizer against Pseudomonas aeruginosa PAO1.
- Author
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Orlandi VT, Rybtke M, Caruso E, Banfi S, Tolker-Nielsen T, and Barbieri P
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- Biofilms radiation effects, Boron pharmacology, Microscopy, Confocal, Porphobilinogen analogs & derivatives, Porphobilinogen pharmacology, Pseudomonas aeruginosa radiation effects, Anti-Bacterial Agents pharmacology, Biofilms drug effects, Photosensitizing Agents pharmacology, Pseudomonas aeruginosa drug effects, Pseudomonas aeruginosa physiology
- Abstract
Photodynamic therapy (PDT) combines the use of organic dyes (photosensitizers, PSs) and visible light in order to elicit a photo-oxidative stress which causes bacterial death. GD11, a recently synthesized PS belonging to the boron-dipyrromethene (BODIPY) class, was demonstrated to be efficient against planktonic cultures of Pseudomonas aeruginosa, causing a 7 log unit reduction of viable cells when administered at 2.5 μM. The effectiveness of GD11 against P. aeruginosa biofilms grown in flow-cells and microtiter trays was also demonstrated. Confocal laser scanning microscopy of flow-cell-grown biofilms suggests that the treatment has a biocidal effect against bacterial biofilm cells.
- Published
- 2014
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11. Photoinduced antibacterial activity of two dicationic 5,15-diarylporphyrins.
- Author
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Orlandi VT, Caruso E, Tettamanti G, Banfi S, and Barbieri P
- Subjects
- Anti-Bacterial Agents chemical synthesis, Anti-Bacterial Agents chemistry, Bacteria radiation effects, Microbial Viability drug effects, Microbial Viability radiation effects, Photosensitizing Agents chemical synthesis, Photosensitizing Agents chemistry, Physical Phenomena, Porphyrins chemical synthesis, Porphyrins chemistry, Wastewater microbiology, Anti-Bacterial Agents pharmacology, Bacteria drug effects, Light, Photosensitizing Agents pharmacology, Porphyrins pharmacology
- Abstract
Antimicrobial photodynamic treatment combines the use of photosensitizers (PSs) and visible light to kill bacterial cells. Cationic porphyrins are PSs largely used against bacteria and, among them, those featuring one positive charge on each of the 5,10,15,20-tetraaryl substituent (tetracationic) are the most used. The aim of this study was to synthesize two dicationic 5,15-di(N-alkyl-4-pyridyl)porphyrins, bearing methyl (PS 3) and benzyl (PS 4) N-alkylating groups, and to compare the efficiency in antibacterial photodynamic treatment, upon irradiation with a halogen-tungsten white lamp. The killing efficiency of the PS 4 was constantly found higher than that of the PS 3 against both pure and mixed cultures of laboratory model microorganisms as well as against wild wastewater microflora. The two PSs are comparable as regards singlet oxygen generation, but show a different repartition coefficient; the more lipophilic benzylated PS 4 shows a better interaction with the bacterial cells than the methylated one (PS 3). The data support the hypothesis that an efficient PS-cell binding is required to obtain significant effects. A correlation among cell binding, photoinactivation and PS lipophilicity is suggested., (Copyright © 2013 Elsevier B.V. All rights reserved.)
- Published
- 2013
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12. Synthesis and photodynamic activity of a panel of BODIPY dyes.
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Banfi S, Caruso E, Zaza S, Mancini M, Gariboldi MB, and Monti E
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- Apoptosis drug effects, Boron Compounds chemical synthesis, Boron Compounds toxicity, Cell Line, Tumor, Fluorescent Dyes chemistry, HCT116 Cells, Humans, Light, Photosensitizing Agents chemistry, Photosensitizing Agents toxicity, Singlet Oxygen metabolism, Boron Compounds chemistry, Photosensitizing Agents chemical synthesis
- Abstract
Eight BODIPY dyes were synthesized and used as photosensitizers (PSs) on the human colon carcinoma cell line HCT116. In this panel of molecules, the structure varies in the substituents on pyrrole 2, 6 positions and on the phenyl ring at the indacene 8 position. For these compounds relevant physico-chemical parameters, such as singlet oxygen production, fluorescent quantum yield, absorbance profile and a relative rank of lipophilicity were determined. Our results indicate that some of these novel PSs are very effective in reducing the growth/viability of HCT116 cells when irradiated with a green LED source, whereas they are practically devoid of activity in the dark, up to 5 μM. To evaluate whether cell death is induced under these conditions, flow cytometric analysis of the percentage of apoptotic and autophagic cells was performed on four molecules, chosen for their efficacy/structural characteristics. Our data indicate that phototoxicity likely occurs mainly through apoptotic cell death, whereas autophagy seems to play a minor role in determining cell fate. Furthermore, the relationship between singlet oxygen generation and the PS efficacy is confirmed, thus underscoring the importance of the heavy-atom effect and of the presence of an aryl substituent at dipyrromethene 8 (meso) position. Among the PSs here described, the most efficient BODIPY was successfully tested on three other human cancer cell lines of different tissue origin, MCF7 (breast), A2780 and A2780/CP8 (ovary, sensitive and resistant to cisplatin, respectively), yielding IC(50) values comparable to those obtained on HCT116., (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Published
- 2012
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13. Effect of organic matter on the in vitro photoeradication of Pseudomonas aeruginosa by means of a cationic tetraaryl-porphyrin.
- Author
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Orlandi VT, Caruso E, Banfi S, and Barbieri P
- Subjects
- Antioxidants pharmacology, Cations, Microbial Sensitivity Tests, Anti-Bacterial Agents pharmacology, Photochemotherapy, Photosensitizing Agents pharmacology, Porphyrins pharmacology, Pseudomonas aeruginosa drug effects
- Abstract
Photodynamic therapy is emerging as an antimicrobial alternative approach; the concomitant presence of a photosensitizer (PS), O(2) and visible light induces lethal oxidative damages to bacterial cells. Among Gram-negative bacteria, Pseudomonas aeruginosa seems to be one of the least susceptible to photodynamic treatment. In this study, we evaluated the influence of several experimental conditions on photoeradication of a planktonic culture of P. aeruginosa PAO1 by means of a tetracationic meso-arylsubstituted porphyrin (RM24). Our findings suggest that the photo-oxidative stress induced by RM24 is strictly correlated to the amount of PS bound to the cells that in turn decreases with the increasing concentrations of organic compounds in the medium. The photoeradication is dependent on PS concentrations, cellular density and light dose. RM24 was able to induce oxidative stress by means of singlet oxygen formation, although ROS formation cannot be ruled out. The standardized experimental conditions of the photospot test allowed us to evidence intraspecific PDT sensitivity differences among three strains of P. aeruginosa., (© 2012 Wiley Periodicals, Inc. Photochemistry and Photobiology © 2012 The American Society of Photobiology.)
- Published
- 2012
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14. Photodynamic effects of novel 5,15-diaryl-tetrapyrrole derivatives on human colon carcinoma cells.
- Author
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Gariboldi MB, Ravizza R, Baranyai P, Caruso E, Banfi S, Meschini S, and Monti E
- Subjects
- Apoptosis, Cell Line, Tumor, Humans, Nitric Oxide metabolism, Photochemotherapy, Photosensitizing Agents chemistry, Porphyrins chemistry, Reactive Oxygen Species metabolism, Singlet Oxygen metabolism, Carcinoma drug therapy, Colonic Neoplasms drug therapy, Photosensitizing Agents toxicity, Porphyrins toxicity
- Abstract
Preliminary in vitro cytotoxicity studies on a panel of meso diaryl-substituted tetrapyrrole derivatives newly synthesized in our laboratory have shown that these compounds are photodynamically active on the human colon carcinoma cell line HCT116. In the present study, we investigate some mechanistic aspects of the photodynamic action of the most active compounds in the series, namely the 5-phenyl-15-(3-methoxyphenyl)porphyrin (1), the 5-phenyl-15-(3-hydroxyphenyl)porphyrin (2) and the 5,15-diphenylporphyrin (3). The results of the cytotoxicity studies indicate that the novel photosensitisers (PSs) are more potent in vitro than m-THPC (Foscan), a powerful PS already approved for clinical use in photodynamic therapy (PDT). A series of experiments were performed to elucidate a number of aspects in the mechanism of PS-induced phototoxicity, including, intracellular accumulation and subcellular localization of the PSs, induction of apoptosis, and generation of reactive oxygen species (ROS) and NO*. All the compounds tested exhibit similar singlet oxygen quantum yields; differential intracellular accumulation can contribute to the observed differences in phototoxicity. Flow cytometric studies indicate that all the tested compounds induce apoptosis; however, their cytotoxic effect does not seem to rely solely on this process. Generation of significant amounts of reactive oxygen species (ROS) and NO* were also observed; however, the contribution of this latter effect to the overall phototoxicity is unclear. Taken together, our observations suggest that the diaryl derivatives included in the present study could represent promising leads for the development of novel photosensitizing agents.
- Published
- 2009
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15. Antibacterial activity of tetraaryl-porphyrin photosensitizers: an in vitro study on Gram negative and Gram positive bacteria.
- Author
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Banfi S, Caruso E, Buccafurni L, Battini V, Zazzaron S, Barbieri P, and Orlandi V
- Subjects
- Cell Survival drug effects, Cell Survival radiation effects, Dose-Response Relationship, Drug, Escherichia coli cytology, Escherichia coli radiation effects, Light, Microbial Sensitivity Tests, Photosensitizing Agents chemistry, Porphyrins chemistry, Pseudomonas aeruginosa cytology, Pseudomonas aeruginosa radiation effects, Staphylococcus aureus cytology, Staphylococcus aureus radiation effects, Structure-Activity Relationship, Escherichia coli drug effects, Photosensitizing Agents pharmacology, Porphyrins pharmacology, Pseudomonas aeruginosa drug effects, Staphylococcus aureus drug effects
- Abstract
Background: Photodynamic therapy exploits visible light and photosensitizers to inactivate cells and this methodology is currently used for the treatment of several types of malignancy. Although various tumours are successfully treated with PSs and light, the application on microorganisms (photodynamic antimicrobial chemotherapy) has not yet found specific medical applications and still remains an open field of fundamental research., Purpose: The assessment of the effect of a panel of seven tetraaryl-porphyrins, two commercial (PS 1 and 2) and five synthetic (PS 3-7) in in vitro experiments against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus., Methods: Three of the new photosensitizers (PS 3, 4 and 5) are tetracationic porphyrins and were prepared by N-alkylation of 5,10,15,20-tetra-4-pyridylporphyrin with a large excess of different benzyl chlorides; compound 7 is a dicationic porphyrin and was obtained in a similar way using a lower excess of 4-methoxybenzyl chloride. The neutral porphyrin (PS 6) was previously described. Dose-response curves were obtained titrating the survivors of cell suspensions (10(8)cfu/ml) exposed to the PSs and irradiated with visible light (total fluence rate 266 J/cm2)., Results: The non ionic porphyrin 6 was the least active PS against all the tested bacteria. Cationic PSs 3, 4, 5 and 7 were more active than the commercial 1 and 2. The Gram positive S. aureus was more sensitive to all the PSs than the Gram negative E. coli and P. aeruginosa, the latter being the more resistant one. Compound 7 was found particularly efficient against P. aeruginosa, causing a 7 log units reduction of survivors at a concentration of 8 microM., Conclusions: The reported results confirm that the presence of positively charged groups on porphyrin frame is fundamental for PSs antibacterial activity, however our data suggest that a moderate degree of lipophilicity, achievable by the introduction of aromatic hydrocarbon side chains on the pyridyl moieties, may improve PSs efficiency. Furthermore dicationic porphyrin 7 seems to be more efficient than the corresponding tetracationic derivatives thus emphasizing an interesting feature involved in the PSs activity.
- Published
- 2006
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16. Comparison between 5,10,15,20-tetraaryl- and 5,15-diarylporphyrins as photosensitizers: synthesis, photodynamic activity, and quantitative structure-activity relationship modeling.
- Author
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Banfi S, Caruso E, Buccafurni L, Murano R, Monti E, Gariboldi M, Papa E, and Gramatica P
- Subjects
- Cell Line, Tumor, Drug Screening Assays, Antitumor, Humans, Photosensitizing Agents chemistry, Photosensitizing Agents pharmacology, Porphyrins chemistry, Porphyrins pharmacology, Photochemotherapy, Photosensitizing Agents chemical synthesis, Porphyrins chemical synthesis, Quantitative Structure-Activity Relationship
- Abstract
The synthesis of a panel of seven nonsymmetric 5,10,15,20-tetraarylporphyrins, 13 symmetric and nonsymmetric 5,15-diarylporphyrins, and one 5,15-diarylchlorin is described. In vitro photodynamic activities on HCT116 human colon adenocarcinoma cells were evaluated by standard cytotoxicity assays. A predictive quantitative structure-activity relationship (QSAR) regression model, based on theoretical holistic molecular descriptors, of a series of 34 tetrapyrrolic photosensitizers (PSs), including the 24 compounds synthesized in this work, was developed to describe the relationship between structural features and photodynamic activity. The present study demonstrates that structural features significantly influence the photodynamic activity of tetrapyrrolic derivatives: diaryl compounds were more active with respect to the tetraarylporphyrins, and among the diaryl derivatives, hydroxy-substituted compounds were more effective than the corresponding methoxy-substituted ones. Furthermore, three monoarylporphyrins, isolated as byproducts during diarylporphyrin synthesis, were considered for both photodynamic and QSAR studies; surprisingly they were found to be particularly active photosensitizers.
- Published
- 2006
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17. Photodynamic effects of porphyrin and chlorin photosensitizers in human colon adenocarcinoma cells.
- Author
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Banfi S, Caruso E, Caprioli S, Mazzagatti L, Canti G, Ravizza R, Gariboldi M, and Monti E
- Subjects
- Cell Line, Tumor, Cell Survival drug effects, Cell Survival physiology, Dihematoporphyrin Ether chemistry, Dihematoporphyrin Ether pharmacology, Dihematoporphyrin Ether therapeutic use, Dose-Response Relationship, Drug, Humans, Photosensitizing Agents chemistry, Photosensitizing Agents pharmacology, Porphyrins chemistry, Porphyrins pharmacology, Adenocarcinoma drug therapy, Colonic Neoplasms drug therapy, Photosensitizing Agents therapeutic use, Porphyrins therapeutic use
- Abstract
Photodynamic therapy (PDT) is a cancer treatment involving systemic administration of a tumor-localizing photosensitizer; this, when activated by the appropriate wavelength of light, interacts with molecular oxygen to form a toxic, short-lived species known as singlet oxygen, which is thought to mediate cellular death. Photofrin, a complex mixture of porphyrin oligomers has recently received FDA approval for the photodynamic treatment of esophageal and endobronchial carcinoma, but its photodynamic and toxicity profiles are far from ideal. In the present study we evaluated a series of porphyrin-based PSs, some of which newly synthesized by our group, with the aim to identify agents with more favorable characteristics. For the most effective compounds in the porphyrin series, chlorin analogs were also synthesized; for comparison, the screening also included Photofrin. Cytotoxicity studies were performed by the MTT assay on a cultured human colon adenocarcinoma cell line (HCT116); the results indicate that the 3,4,5-trimethoxyphenyl, 3OH- and 4OH-phenyl, and the sulfonamidophenyl derivatives are significantly more potent than Photofrin. Flow cytometric studies and fluorescence microscopy indicate that in PDT-treated HCT116 cells death occurs mainly by apoptosis. In summary, novel PSs described in the present study, belonging both to the porphyrin and chlorin series, have proven more effective than Photofrin in killing colon cancer cells in vitro; extending these observation to in vivo models, particularly regarding the deeper reaching chlorin derivatives, might lead to significant advances in the development of tumor PDT.
- Published
- 2004
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18. Oxidative cleavage of plasmid bluescript by water-soluble Mn-porphyrins and artificial oxidants or molecular oxygen.
- Author
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Banfi S, Cassani E, Caruso E, and Cazzaro M
- Subjects
- Catalysis, Cell Line, Tumor, Genetic Vectors, Humans, Hydrogen-Ion Concentration, Manganese chemistry, Manganese metabolism, Manganese Compounds chemistry, Metalloporphyrins pharmacology, Oxidation-Reduction, Plasmids chemistry, Solubility, Water chemistry, Manganese Compounds metabolism, Metalloporphyrins metabolism, Oxidants metabolism, Oxygen metabolism, Plasmids metabolism
- Abstract
A set of eight Mn(III)-porphyrins were used as catalysts in oxidative demolition of Plasmid Bluescript, to the nicked and linear forms, in the presence of different oxygen donors (NaOCl, H(2)O(2), AcOOH, t-BuOOH). The efficiency of the catalytic system is related to a combination of factors such as porphyrin structures, pH of the aqueous phase and nature of the primary oxidant. The highest catalytic activity was observed when ionic porphyrins were used as catalyst (the cationic being more active than the anionic) and NaOCl was used as primary oxidant at pH 9.5; in contrast, lipophilic catalysts proved to be completely unreactive towards the DNA, whichever oxidant used. The plasmid demolition was also achieved by irradiating the reaction mixture, containing Zinc porphyrins, with a white lamp; under these conditions, the highest efficiency was again observed with meso-tetra(1-methyl-4-pyridyl)porphyrin. However, preliminary experiments of photo activation applied on tumour cells (HCT 116) showed no dead cells with cationic porphyrin, while the amphiphilic Zn-tetra(4-hydroxyphenyl)porphyrin gave IC(50) values at 5 x 10(-2) microM concentration (37.1 ng/mL).
- Published
- 2003
- Full Text
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