1. Impact of purification and fractionation process on the chemical structure and physical properties of locust bean gum.
- Author
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Sébastien G, Christophe B, Mario A, Pascal L, Michel P, and Aurore R
- Subjects
- Chemical Fractionation methods, Galactans isolation & purification, Galactose analogs & derivatives, Mannans isolation & purification, Plant Gums isolation & purification, Galactans chemistry, Mannans chemistry, Plant Gums chemistry
- Abstract
Crude locust bean gum (CLBG) was purified and fractionated into two parts: the first was obtained by solubilization in water at 25°C (GM25) and the second consisted in a further extraction at 80°C on the residual impoverished fraction (GM80). The complete structural characterization has shown that GM80 possessed relatively longer chain lengths than GM25, a slightly lower degree of galactose substitution and a somewhat sharper galactosyl distribution in substituted and unsubstituted regions. A physical behavior analysis was carried out on solubilization kinetics, viscosity, viscoelasticity and formation of associated gels with xanthan or carrageenan. The average structure of GM80 generated larger intra-chain, inter-chain and inter-molecular interactions, resulting in the appearance of a stronger network. Small structural differences therefore generated very different physical behaviors. This study thus allowed to establish, in a precise and complete manner, fractionation-purification-structure-function relationships of galactomannans extracted from carob., (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Published
- 2014
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