1. A novel thermophilic recombinant obligate xylobiohydrolase (AcGH30A) from Acetivibrio clariflavus orchestrates the deconstruction of xylan polysaccharides.
- Author
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Singh YR, Thakur A, Fontes CMGA, and Goyal A
- Subjects
- Hydrogen-Ion Concentration, Temperature, Substrate Specificity, Hydrolysis, Bacterial Proteins chemistry, Bacterial Proteins genetics, Bacterial Proteins metabolism, Bacterial Proteins isolation & purification, Cloning, Molecular, Escherichia coli genetics, Xylans chemistry, Xylans metabolism, Recombinant Proteins chemistry, Recombinant Proteins metabolism, Recombinant Proteins genetics, Recombinant Proteins isolation & purification, Enzyme Stability
- Abstract
GH30 xylobiohydrolases, an expanding enzyme category, need deeper insights for optimal use. The primary aim of this study was to characterize a new xylobiohydrolase, AcGH30A of GH30 family from Acetivibrio clariflavus. The gene encoding AcGH30A was cloned using pET28a(+) vector and expressed in E. coli BL21(DE3) cells. AcGH30A was purified by immobilized metal-ion affinity chromatography. SDS-PAGE analysis of AcGH30A showed molecular mass of ~58 kDa. AcGH30A showed optimum temperature 80 °C and optimum pH 7.0. AcGH30A was stable (maintaining >80 % of control activity) in pH range, 4-7 and temperature range, 30 °C -70 °C when incubated for 90 min. AcGH30A displayed melting temperature, 72 °C and half-life, 21 days at 4 °C. The enzyme activity of AcGH30A was enhanced by 10 mM Ca
2+ and Mg2+ ions by 25 % and 21 %, respectively, whereas 10 mM Co2+ , Zn2+ , Fe2+ , and Cu2+ ions significantly reduced it. AcGH30A showed activity against various xylan polysaccharides displaying highest Vmax , 139 U.mg-1 and KM , 0.71 mg.ml-1 against 4-O-methyl glucuronoxylan under optimum conditions. TLC, HPLC and LC-MS analyses of AcGH30A hydrolyzed products from xylan substrates revealed the release of sole product, xylobiose, confirming it as an obligate xylobiohydrolase. AcGH30A being a highly thermostable enzyme can be potentially utlilized in various biotechnological applications., Competing Interests: Declaration of competing interest CMGAF is a financial beneficiary of the company that sells the enzyme that is described in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)- Published
- 2024
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