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Background: Nitrogen (N) availability is crucial in regulating plants' abiotic stress resistance, particularly at the seedling stage. Nevertheless, plant responses to N under salinity conditions may vary depending on the soil's NH 4 + to NO 3 - ratio., Methods: In this study, we investigated the effects of different NH 4 + :NO 3 - ratios (100/0, 0/100, 25/75, 50/50, and 75/25) on the growth and physio-biochemical responses of soybean seedlings grown under controlled and saline stress conditions (0-, 50-, and 100-mM L - 1 NaCl and Na 2 SO 4 , at a 1:1 molar ratio)., Results: We observed that shoot length, root length, and leaf-stem-root dry weight decreased significantly with increased saline stress levels compared to control. Moreover, there was a significant accumulation of Na + , Cl - , hydrogen peroxide (H 2 O 2 ), and malondialdehyde (MDA) but impaired ascorbate-glutathione pools (AsA-GSH). They also displayed lower photosynthetic pigments (chlorophyll-a and chlorophyll-b), K + ion, K + /Na + ratio, and weakened O 2 •- -H 2 O 2 -scavenging enzymes such as superoxide dismutase, catalase, peroxidase, monodehydroascorbate reductase, glutathione reductase under both saline stress levels, while reduced ascorbate peroxidase, and dehydroascorbate reductase under 100-mM stress, demonstrating their sensitivity to a saline environment. Moreover, the concentrations of proline, glycine betaine, total phenolic, flavonoids, and abscisic acid increased under both stresses compared to the control. They also exhibited lower indole acetic acid, gibberellic acid, cytokinins, and zeatine riboside, which may account for their reduced biomass. However, NH 4 + :NO 3 - ratios caused a differential response to alleviate saline stress toxicity. Soybean seedlings supplemented with optimal ratios of NH 4 + :NO 3 - (T3 = 25:75 and T = 4 50:50) displayed lower Na + and Cl - and ABA but improved K + and K + /Na + , pigments, growth hormones, and biomass compared to higher NH 4 + :NO 3 - ratios. They also exhibited higher O 2 •- -H 2 O 2 -scavenging enzymes and optimized H 2 O 2 , MDA, and AsA-GSH pools status in favor of the higher biomass of seedlings., Conclusions: In summary, the NH 4 +  and NO 3 - ratios followed the order of 50:50 > 25:75 > 0:100 > 75:25 > 100:0 for regulating the morpho-physio-biochemical responses in seedlings under SS conditions. Accordingly, we suggest that applying optimal ratios of NH 4 + and NO 3 - (25/75 and 50:50) can improve the resistance of soybean seedlings grown in saline conditions., (© 2024. The Author(s).)

Soil salinity caused a widespread detrimental issue that hinders productivity in agriculture and ecological sustainability, while waste-derived soil amendments like biochar have drawn attention for their capacity to act as a mitigating agent, by enhancing the physical and chemical features of soil, and contributing to the recovery of agricultural waste resources. However, the information concerning biochar and salinity which affect the physicochemical characteristics of soils, crop physiology, and growth is limited. To investigate whether biochar mitigates the salinity stress on wheat crop seedlings, we grow them with salinity stress (120 mM), and biochar (20 tons ha -1 ), and its interactive effects. The soil properties of soil organic carbon (SOC), soil organic matter (SOM), dissolved organic carbon (DOC), and soil available phosphorus (SAP) decreased in the saline soil by 36.71%, 46.97%, 26.31%, and 15.00%, while biochar treatment increased SOC, DOC, and SAP contents by 7.42%, 31.57%, and 15.00%, respectively. On the other hand, dissolved organic nitrogen (DON) contents decreased in all the treatments compared to the control. The root growth traits, SPAD values, leaf nitrogen, photosynthetic parameters, antioxidant enzymes, and reactive oxygen species decreased in the saline treatment while increasing in the biochar and interactive treatment. Thus, these activities resulted in higher leaves and root biomass in the biochar treatment alone and interactive treatment of salinity and biochar. According to principal component analysis, redundancy analysis, and the mantel test, using biochar in conjunction with salinity treatment was found to be more effective than salinity treatment alone. The results of this study suggest that biochar can be used as a sustainable agricultural technique and a means of mitigation agent by lowering soil salinity while increasing the biomass of crops.

A field experiment was conducted during the Rabi season 2017-2018 (October-March) at the University of Agriculture, Peshawar research farm to examine the influence of different nitrogen (N) and phosphorus (P) levels on two different oat varieties: Australian and Ukrainian. The treatments included control and three levels of nitrogen and phosphorus at 30, 60, and 90 kg ha -1 . The treatments were arranged in randomized complete block design (RCBD) and replicated three times. The findings showed that the oat varieties were significantly different from one another in yield and yield parameters. The Australian variety recorded higher emergence (49 plants m -2 ), days to emergence (15 days), days to flowering (122 days), days to maturity (145 days), plant height (142.7 cm), number of leaves (6.03 leaves plant -1 ), number of tillers (92.2 tillers m -1 ), biological yield (8,179.2 kg ha -1 ), and grain yield (3,725.6 kg ha -1 ) than the Ukrainian variety. Similarly, different N and P levels, the maximum days to emergence, days to flowering, and days to maturity were recorded in a control plot. The application of 105 kg N + 90 kg P ha -1 was statistically similar to the application of 105 kg N + 60 kg P ha -1 . Maximum emergence (60 plants m -2 ), number of leaves (7.0 leaves plant -1 ), plant height (118.6 cm), number of tillers m -1 (102.6), biological yield (9,687.5 kg ha -1 ), and grain yield (4,416.7 kg ha -1 ) were determined in Australian variety. Based on the findings of this study, the Australian variety performed better in terms of yield and yield components and the application of N and P fertilizers at the rate of 105 kg N + 60 kg P ha -1 produced the best results in both oat varieties., Competing Interests: Aman Ullah is an Academic Editor for PeerJ., (© 2021 Bibi et al.)

Leaf nitrogen (N) concentration plays an important role in biochemical and physiological functions, and N availability directly influences rice yield. However, excessive N fertilization is considered to be a root cause of environmental issues and low nitrogen use efficiency. Therefore, the selection of appropriate nutrient management practices and organic amendments is key to maximizing nitrogen uptake and maintaining high and sustainable rice production. Here, we evaluated the effects of different 15 N-labelled nitrogen sources (urea, ammonium nitrate, and ammonium sulfate at 315 kg ha -1 ) with or without biochar (30 t ha -1 ) on paddy soil properties, root growth, leaf gas exchange, N metabolism enzymes, and N uptake in the early and late seasons of 2019. We found significant differences among N fertilizer sources applied with or without biochar ( P  < 0.05). Across the seasons, the combination of biochar with N fertilizers significantly increased soil organic carbon by 51.21% and nitrogen availability by 27.51% compared with N fertilizers alone. Correlation analysis showed that rice root morphological traits were strongly related to soil chemical properties, and higher root growth was measured in the biochar treatments. Similarly, net leaf photosynthetic rate averaged 9.34% higher, chlorophyll (Chl) a concentration 12.91% higher, and Chl b concentration 10.05% higher in the biochar treatments than in the biochar-free treatments across the seasons. Notably, leaf 15 N concentration was 23.19% higher in the biochar treatments in both seasons. These results illustrated higher activities of N metabolism enzymes such as NR, GS, and GOGAT by an average 23.44%, 11.26% and 18.16% in the biochar treatments across the seasons, respectively. The addition of biochar with synthetic N fertilizers is an ecological nutrient management strategy that can increase N uptake and assimilation by ameliorating soil properties and improving the morpho-physiological factors of rice., Competing Interests: The authors declared that there is no conflict of interest., (© 2021 The Authors. Published by Elsevier B.V. on behalf of King Saud University.)

Silybum marianum L. is medicinally important for its active principle component silymarin. Silymarin regenerates damaged hepatic tissues. On the basis of such regenerative properties, the radical scavenging activity (1,1-diphenyl-2-picrylhydrazyl (DPPH)) of different tissues and the phenotypic difference of the hepatoprotective species, S. marianum L. were evaluated. There was less phenotypic difference in purple and white varieties of S. marianum. Assay of the antioxidant potential of different parts of the plant revealed that significantly higher activity (78.2%) was observed in seeds of the purple flowering plant than seeds of white flowering plant (49%) after different time intervals. Young leaves collected from white flowering plant exhibit 64.8% activity, which is higher than the purple flowering plant (55.1%). Significantly, same activity was observed in mature leaves of white (52%) and purple flowering plants (50%). The main stem collected from both the varieties exhibits similar activity from 50 to 52%. A 67.2% activity was recorded for mature roots of white flowering plant followed by roots of the purple variety (65%). The present study revealed that seeds and roots of both the varieties scavenge and detoxify more DPPH free radicals than other plant parts and can be used as a source of natural antioxidants and food additives.