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Coupled solid carbon source biofilm carriers (CCBs) was usually utilized to enhance the treatment efficiency of low carbon/nitrogen (C/N) wastewater. However, current CCBs have low carbon release capacity because of its small inner mass transfer coefficient. Therefore, this study innovatively applied pore-forming methods to modify CCBs. After orthogonal selections, two porous CCBs, which were respectively prepared through circulating freezing pore-forming method (CCB2) and ammonium bicarbonate pore-forming method (CCB3), were proposed and further applied in sequencing batch moving bed biofilm reactors (SBMBBRs). The results indicated that circulating freezing pore-forming method could improve the mechanical strength and carbon source release rate of CCBs. In addition, CCB2 could significantly enhance the total nitrogen (TN) removal efficiency of SBMBBRs, when compared with the non-porous CCBs (i.e., CCB1). Further biofilm and simultaneous nitrification and denitrification (SND) rate calculation attributed this enhancement to the higher biofilm amount (i.e., 0.06 g g -1 CCB) and the higher SND rate (i.e., 33.60%). Microbial community analysis reiterated these observations that CCB2 and CCB3 could accumulate Proteobacteria, Actinobacteriota and Nitrospirota, and also stimulate nitrification and denitrification associated pathways. More importantly, the cost calculation indicated CCB2 cost only 47.37% of CCB1 and 31.34% of CCB3, showing highly economic applicability. Overall, our results collectively proved that CCBs manufactured by circulating freezing pore-forming method could provide more carbon releasing points and microorganisms attaching positions, exhibiting effectively nitrogen removal when treating low C/N wastewater., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022. Published by Elsevier Ltd.)

Low carbon/nitrogen ratio (C/N) wastewater is widespread and difficult to treat. To find a resolution to this issue, this study systematically evaluated the constituents of composite solid carbon (i.e., skeletons, carbon sources and crosslinking agents), and proposed a new multi-carbon source composite S1 (MCSC.S1). The effects on nitrogen removal were further determined through a sequencing batch moving bed biofilm reactor (SBMBBR). The results showed that MCSC.S1, which was composed of polyvinyl alcohol-sodium alginate (PVA-SA), corncob + poly (R-β-hydroxybutyrate) (CC + PHB), and H 3 BO 3 -4% CaCl 2 +Na 2 SO 4 had high stability and absorption. With MCSC.S1, total nitrification removal was enhanced by more than 48.56% through releasing carbon and absorbing the attached denitrifying bacteria. In addition, it was found that MCSC.S1 can simulate the simultaneous nitrification and denitrification (SND) process and contribute to 29.85% of the total nitrogen removal. 16S gene-based analysis attributed this supplementary nitrogen removal to the enrichment of nitrification (i.e., Proteobacteria, Actinobacteria and Chloroflexi), denitrification of associated bacteria (i.e., Nitrospirota) in MCSC.S1 added reactor, and the increase in nitrogen recycling associated genes. These findings collectively demonstrate that the new MCSC.S1 could effectively enhance nitrogen removal efficiency in low C/N ratio wastewater., (Copyright © 2021. Published by Elsevier Ltd.)

Electrotrophic denitrification is a promising novel nitrogen removal technique. In this study, the performance and the mechanism of electrotrophic denitrification coupled with sulfate-sulfide cycle were investigated under different anodic influent COD/SO 4 2- ratios. The results showed that electrotrophic denitrification contributed to more than 22% total nitrogen removal in cathode chamber. Higher COD/SO 4 2- ratios would deteriorate the sulfate reduction but enhance methane production. Further mass balance indicated that the electron flow utilized by methanogenic archaea (MA) increased while that utilized by sulfate-reducing bacteria (SRB) decreased as the COD/SO 4 2- ratio increased from 0.44 to 1.11. However, higher COD/SO 4 2- ratios would produce more electrons to strengthen electrotrophic denitrification. Microbial community analysis showed that the biocathode was predominantly covered by Thiobacillus that encoded with narG gene. These findings collectively suggest that electrotrophic denitrification could be a sustainable approach to simultaneously remove COD and nitrogen under suitable COD/SO 4 2- ratio based on sulfur cycle in wastewater., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Anaerobic digestion (AD) is regarded as an effective method to treat waste activated sludge (WAS) and fat, oil and grease (FOG). Co-digestion of WAS/FOG could promote the methane yield but it will cause acid and salinity inhibition. Green waste (GW) was added into the digesters, and its effects on co-digestion of WAS and FOG in the mesophilic batch digester were investigated. Digestive performances (such as hydrolysis, acidogenesis and methanogenesis) were studied emphatically. The results showed that digester L6 (WAS:FOG:GW = 1:2:1, VS basis) presented the highest specific methane yield (SMY, 341.5 mL/g VS). The results of kinetics study verified that there was a slower hydrolysis rate when GW was applied as a co-substrate, which could reduce the potential of acid inhibition. Volatile fatty acid (VFA) and electrical conductivity analysis showed that GW addition could keep moderate VFA concentrations and alleviate the negative effects of high-salinity substrates on the digestive systems. The microbial community and diversity analysis proved that GW addition was beneficial to keep the balance of hydrolytic bacteria, acidogens and acetogens. The results of this study indicated that GW addition could enhance the energy recovery and system stability in the WAS/FOG co-digestive system.

The collapse of dense algal blooms is identified as a significant source of methane (CH 4 ) emissions. When flocculation is used for algae removal, algal carbon is often turned into CH 4 and carbon dioxide (CO 2 ). Here, we established a "bio-pump" to control algal blooms and reduce greenhouse gas (GHG) emissions by the introduction of submerged macrophytes to the aquatic ecosystem and combination of flocculation and capping. The results suggested that this strategy contributed to an approximately 98% algae removal and sustainably improved dissolved oxygen (DO) in the water and sediment after the 40-day incubation. The aerobic condition at the sediment-water interface and deeper oxygen penetration in the sediment inhibited the abundance of microorganisms related to anaerobic CH 4 production, then changed the metabolic pathway and fate of algal carbon. After the 40-day incubation, compared with flocculation-capping treatments, the bio-pump reduced 69.07% CH 4 and 77.57% CO 2 emissions, which was jointly contributed by the inhibition of anaerobic CH 4 production, aerobic oxidation of CH 4 and carbon sequestration of submerged macrophytes. This was also demonstrated from the finding of a decrease in methyl coenzyme M reductase (mcrA) gene, an increase in particulate methane monooxygenase (pmoA) gene and the absorption of 13 C-labeled from algae biomass by submerged macrophytes at the end of incubation. Therefore, the bio-pump established in the present study can improve DO in algal blooms water and turn algal-derived organic matter into the plant biomass, which supplied a sustainable method for algae removal and GHG reduction., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

This paper used cetyltrimethylammonium chloride (CTAC) pre-loaded activated carbon (AC) to research nitrate adsorption. Effects of various parameters such as AC types, AC dosage as well as initial pH were studied. The results indicated that the ACs modified by CTAC can get higher nitrate removal. Even pH is neutral and basic, an accepted removal about 2.5 mg/g can be observed. The more CTAC pre-loaded on the AC surface, the higher nitrate adsorption capacity can be obtained. pH is regarded as a key factor affecting interactions between adsorbent and adsorbate, and the results confirmed that the nitrate adsorption on modified AC decreases gradually with the growth of initial pH. Besides, the acidic pH condition is much favoured for adsorption while the results gained a nitrate adsorption about 4.28 mg/g at pH = 3 condition. Sorption mechanism of nitrate on CTAC modified AC was investigated through two kinetic modellings including pseudo-second-order and Weber and Morris intra-particle diffusion model. The results imply that the generalized kinetic models tally well with experimental data. Additionally, interference of co-existing anions is examined, and the results showed that higher co-anions concentration would bring a heavier depression of the nitrate uptake due to its competing for adsorption sites.

Anodic mixotrophic denitrification microbial fuel cell (MFC) was developed for pollutants removal and electricity generation in treatment of low C/N domestic wastewater. The experimental results show that the MFC achieved up to 100% of acetate, 100% of sulfide, and more than 91% of nitrate removal efficiency in all the MFCs. Particularly, thiosulfate was generated as the main intermediate of sulfide oxidation, and the sulfate generation ratio ranged from 66.93% to 73.76%. Those electrons produced during the acetate and sulfide oxidation were mainly used for denitrification and electricity generation. The microbial community analysis revealed that heterotrophic denitrifying bacteria (HDB) and sulfide-based autotrophic denitrifying bacteria (SADB) were the dominant bacteria for pollutants removal, and those facultative autotrophic bacterium (FAB) were key functional genera for high sulfate generation under both low and high sulfide concentrations. Meanwhile, the microbial functional prediction revealed that sulfide oxidation gene of Sqr and Sox were highly expressed. Moreover, a preliminary sulfide-based autotrophic denitrification (SAD) potential estimation indicated that the sulfide generated in the WWTPs had great potential for denitrification., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)

The processes of (n)ZVI/AC（（nano）zero valent iron/activated carbon）micro-electrolysis were applied for nitrate removal from groundwater, aiming to reduce nitrate to N 2 , an environmentally friendly end product. (n)ZVI was utilized and combined with selected commercial AC to form the micro-electrolysis. Effect of different operational parameters, including reductant dosage, (n)ZVI/AC ratios, and reaction pH, on nitrate and TN removal were cleared. The results showed that nZVI presents higher reaction activities than ZVI in micro-electrolysis. With the increase of the (n)ZVI/AC mass ration from 1:2 to 2:1, the TN removal increased from 16.8% to 38.9%, then declined with the further increase of the ratio. The nitrate removal was negatively correlated with the initial pH of the solution. Compared to ZVI particles, even with a lower dosage, nZVI presented 100% nitrate removal at acidic and neutral pH conditions, and TN removal could maintain higher than 35% with pH lower than 9.0. A kinetic model was also established to explain the pathways of nitrate removal, and the results indicated that AC not only enriched nitrate as an adsorbent but also present highly potential in catalytic converting nitrate to N 2 . The technique presented great potentials in removing nitrate from water and a promising application prospect., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

The effects of harmful algal blooms (HABs) on nutrient dynamics have been extensively studied; however, the response of nitrogen to continuous HAB degradation and subsequent reoccurrence is not well understood. Here, a small-scale experiment was conducted to assess how nitrogen in the sediment-water interface (SWI) responds to HAB degradation and subsequent reoccurrence at different initial algal densities. The results showed that during the algae decomposition stage, the NH 4 + -N flux of the SWI remained positive but decreased with the increase in algal density from 3.5 × 10 7 to 2.3 × 10 8 cells per L, indicating that the sediment was the source of NH 4 + -N. In contrast, the deposit was a sink of NO 3 - -N. However, during the reoccurrence of HAB, the distribution of NH 4 + -N and NO 3 - -N fluxes was completely converted. Nitrogen flux analysis throughout algae decomposition and reoccurrence indicated that although the sediment acted as a sink of nitrogen, the flux was dependent on the initial algal density. Our results confirmed that algae decomposition and reoccurrence would greatly affect the nitrogen cycle of the SWI, during which dissolved oxygen (DO) and initial algal density dominated. This study is the first to show that the regulation of nitrogen flux and migration changes during continuous HAB decomposition and subsequent reoccurrence., Competing Interests: The authors declare no competing financial interests., (This journal is © The Royal Society of Chemistry.)

It has been proved that the nitrogen can be removed from the sediment in a sediment microbial fuel cell system (SMFCs), but the competition between nitrate and oxygen for electrons would be a key factor that would affect the removal efficiency, and its mechanism is not clear. Based on organic sediment fuel, an SMFC was constructed, and the influence of dissolved oxygen (DO) on nitrogen transformation and cathodic microbial communities was investigated. The results showed that the best total nitrogen removal efficiency of 60.55% was achieved at DO level of 3 mg/L. High DO concentration affected the removal efficiency through the electrons' competition with nitrate, while low DO concentration suppressed the nitrification. Comamonas, Diaphorobacter and Brevundimonas were the three dominant genera responsible for denitrification at DO concentration of 3 mg/L in this study. The establishment of SMFCs for nitrogen removal by regulating DO level would offer a promising method for sediment treatment.

This study aims to enhance hydrolysis and anaerobic digestion of corn cob (CC) by using food waste (FW) pretreatment. FW, which tends to be acidification in fermentation, was applied in this process as an acid-like agent to accelerate lignocellulose hydrolysis, aiming to promote methane yield in further digestion process. The effect of FW pretreatment on pH, soluble chemical oxygen demand (SCOD), volatile fatty acids (VFAs), cellulose/hemicellulose contents and cellulose crystallinity are specially focused. FW:CC = 1:3 based on volatile solid (VS) was found to be the optimal mixing ratio in pretreatment and its hydrolysis efficiency was 28% higher than the control group. An increase of 13.2% in cellulose reduction and a decrease of 6.7% in cellulose crystallinity was achieved at this ratio. Supplementation of FW increased VFA concentrations in slurry mixture that directly change the activities of enzymes and microorganisms. In the stage of methane production, the digester A3 (FW:CC = 1:6 based on VS) with higher hydrolysis efficiency presented the best performance in methane production with a specific methane yield of 401.6 mL/g·VS, due to the recovery of the pH in this digester to the optimal pH range for methanogens' metabolism (pH 6.3-7.2). Kinetics studies of cellulose/hemicellulose degradation indicated that the pretreatment of FW could improve the degradation of cellulose. Three-dimensional excitation emission matrix (3DEEM) results further confirmed that FW play an important role in lignocellulose hydrolysis. In addition, variations of lignocellulosic textures during the pretreatment were also cleared by using field emission-scanning electron microscopy (FE-SEM) analysis., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

In excess sludge digestion, organic matters cannot be digested adequately due to its high lignocellulose content. This study attempted to comprehensively evaluate the digestive performances of sludge with different lignocellulosic components (hemicellulose, cellulose and lignin). Results show that hemicellulose/dealkaline lignin addition (S6) presents the highest methane yield of 203.6 mL/gVS. Compared to hemicellulose, dealkaline lignin is hardly degraded (lower than 10%), while its participation can promote the degradation of other organics in the system. Additionally, solo cellulose feedstock is difficult to be hydrolyzed (only 40.1%) without hemicellulose and dealkaline lignin addition. VFAs composition analysis indicates that VFA inhibition occurs in the digester with hemicellulose, cellulose and dealkaline lignin addition (S8). Microbial diversities of different digestive systems show that the relative abundance of Euryarchaeota in the digester S6 (7.2%) is much higher than others, and some specific microbes (Bacteroidetas and Firmicutes) are enriched in the S5 (74.1%) and S8 (54.7%) digesters., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

This study evaluated the digestive performance of excess sludge and different crop straws. Methane production, hydrolytic and acidogenic performance of different substrates were specially focused. Results showed that wheat straw presented the highest experimental biochemical methane potential (BMP, 462.5 mL/g VS removal ). Corn cob-added digester obtained the highest VS removal (68.8%) and the lowest methane content due to its high hemicellulose content. Kinetic studies showed that addition of crop straws could shorten the lag phase and accelerate the hydrolysis of substrates. 3DEEM results indicated that lignocellulosic compositions play important roles in sludge hydrolysis, while FE-SEM analysis showed that the lignocellulosic textures of different crop straws determine their hydrolytic performances. Microbial community and diversity results showed that the crop straws in sludge provided abundant nutrients and their unique structures supplied attachment points for microorganisms, which ensured the efficient operation of digestive process., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

In this study, the impact of COD/SO 4 2- ratio in sewage on methane and hydrogen sulfide production in sewer biofilms was investigated by using three identical lab-scale gravity sewer systems. The results showed that the COD/SO 4 2- played a key role in the competition between methanogenic archaea (MA) and sulfate reducing bacteria (SRB). Both the lowest methane and hydrogen sulfide production were obtained at COD/SO 4 2- ratio of 6. The carbon transformation revealed that the activity of both MA and SRB was inhibited at this COD/SO 4 2- ratio. Methanosarcina and Methanobacterium were the two dominant MA, while Desulfonema, Desulfotomaculum and Desulfovibrio were the dominant SRB in this case. The specific SRB activity measured by batch tests proved that acetate was mainly degraded by the MA, while propionate was the preferred substrate for the SRB., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Anthropogenic activities can cause serious negative effects on ecosystems. Despite the ecological significance of bacterial communities, the integrated biogeography of planktonic and sedimentary bacterial communities in response to anthropogenic activities is not adequately understood. Here, we examined environmental parameters and the composition of planktonic and sedimentary bacteria in the Yulin River, a tributary of Three Gorges Reservoir, in response to changes in land use and dam construction. The results revealed that human-induced land use changes enhanced the nutrient concentrations in surface water and dam construction reduced the content of carbon and nitrogen in immediately downstream sediments. Intensified human-dominated land use showed a slight impact on sedimentary bacterial communities but largely reduced the diversity of planktonic bacterial communities. Moreover, human-induced land use changes increased the abundance of genes associated with denitrification, nitrification, and anammox in planktonic bacterial communities by 19.04%, 32.40% and 30.45%, respectively. In dam construction regions, the diversity and nutrient-related metabolic activity of sedimentary bacterial communities immediately downstream of the dam were decreased, whereas these changes were not observed in planktonic bacterial communities. Additionally, bacterial community composition was significantly related to nutrient concentrations variability and followed a distance-decay pattern. Furthermore, environmental effects explained more of the variation in planktonic bacterial community composition as compared with spatial effects did, whereas, sedimentary bacterial communities were more closely related to spatial effects. Our results demonstrated the distinct responses of planktonic and sedimentary bacterial communities to anthropogenic activities, and offered new insight for understanding their potential ecological influence on rivers., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Depleted oxygen (O 2 ) in the sediment and overlying water of malodorous black water poses a potential threat to aquatic ecosystems. This study presents a method for sustainable regulation of the dissolved oxygen (DO) levels towards the malodorous black water. Oxygen-loaded natural porous materials were prepared by vacuum degassing to remove air from the pores and fill them with pure O 2 . Capping anaerobic sediment with the prepared 6 oxygen-loaded porous materials was effective in prompting the DO concentration of the malodorous black water. Although granules activated carbon (GAC) displayed the highest oxygen-loading capability, oxygen-loaded volcanic stone additive was more efficient for long-lasting combating of the anaerobic condition because the DO level at sediment-water interface (SWI) and the DO penetration depth showed approximately 5.38- and 3.75-fold increase, respectively, compared with the untreated systems. The improvement in DO was substantially enhanced in the presence of submerged macrophyte (Vallisneria natans), during which the release of O 2 from oxygen-loaded volcanic stone facilitated the plant growth. With the joint efforts of the O 2 released from volcanic stone and photosynthesis by the macrophytes, the DO levels were maintained at approximately 6.80 mg/L after a 41-day incubation, which exceeded (P < 0.05) the value in only oxygen-loaded volcanic stone or macrophytes added treatments. In addition to the elevated DO level, the combined employment of oxygen-loaded volcanic stone and macrophytes triggered a negative ammonia (NH 4 + -N) flux across the SWI and an 85.82% reduction of methane (CH 4 ) production compared with those without treatment, accompanied by a decrease in total inorganic carbon and a 2.55- fold increasing of submerged macrophyte biomass, which is presumably attributed to nitrification, remineralization, and assimilation. The results obtained here shed a degree of light on the sustainable modulation of the anaerobic condition in malodorous black water., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

There are large amounts of waste activated sludge (WAS) and kitchen waste (KW) produced every year in China. It has been confirmed that anaerobic co-digestion is an effective method to solve this problem. The targets of the present study were optimizing the digestive performances and clearing of the mechanism of bentonite addition by adding bentonite into digestive system. Group M (WAS: KW = 1:2, based on VS) presented higher cumulative methane yield (CMY), where the CMY increased from 19.8 to 36.3 mL/g VS with the bentonite dosage from 0 to 2 g/g VS. After bentonite addition, the lag phase of every digester presented an obvious decrease from 15.1 to 1.4 d. Furthermore, and the moderating effects on microbial community by bentonite. The addition of bentonite improved methane production, and it can also reduce the lag phase of methane production in co-digestion. What's more, bentonite addition increased the speed of pH recovery from 4.2-4.8 to normal level (7.0-8.0) and thus enhanced the system stability. The conclusion of this study can be used to guide practical engineering., Competing Interests: The authors have declared that no competing interests exist.

Carbon sources are a critical requirement for the proliferation of algae and the occurrence of harmful algal blooms (HABs), but are often turned into methane (CH 4 ) after the collapse of severe HABs. Here, we attempt to remove HABs, reduce algal-derived CH 4 emissions, and repair the broken carbon biogeochemical cycle in aquatic systems using an integrated ecological approach including flocculation, capping, and submerged macrophyte induction, preliminary at a microcosm scale. This strategy sustainably reached 98% algal removal after 65 days of incubation and resulted in an aerobic microenvironment (ORP = +12 mv) at the sediment-water interface. The approach contributed to an approximate 60% decline in CH 4 released from the aquatic environment into the atmosphere jointly through assimilation of mineralized organic carbon by submerged macrophytes, production of carbon dioxide (CO 2 ) under aerobic conditions, and aerobic CH 4 oxidation. Some of the CO 2 produced in the aquatic phase contributed to inorganic carbon and formed the submerged macrophytes biomass. A combination of flocculation, capping, and submerged macrophyte incubation were significant contributors to altering the carbon budget and sealing nearly 99% of the carbon in the simulated ecosystem (the majority in sediment, followed by submerged macrophytes), providing a sustainable way to reuse algal-derived carbon and reduce CH 4 emissions., (Copyright © 2018 Elsevier B.V. All rights reserved.)

The effect of turbulence on phytoplankton growth has been widely studied; however, its effects with respects to suspended particulate matter (SPM) on the development of phytoplankton communities and the behavioral responses of phytoplankton to turbulence and SPM are poorly understood. Here, an approximately homogeneous turbulence simulation system (AHTS, mainly consisting of an oscillating-grid apparatus) was established to gain insight into the mechanisms underlying phytoplankton community responses in turbid, well-mixed waters. The results revealed that maintaining the turbulence dissipation rates (Ɛ) of 2.25 × 10 -3 and 1.80 × 10 -2  m 2 /s 3 caused significant reductions in algal density, and the effects could be substantially enhanced when 500 mg/L of SPM were added before day 12. In contrast to the constant decrease of algal density for the Ɛ of 2.25 × 10 -3  m 2 /s 3 , a dramatic increase in the phytoplankton density occurred after 16 days of incubation for a Ɛ of 1.80 × 10 -2  m 2 /s 3 , irrespective of SPM. Addition of SPM in the Ɛ of 1.80 × 10 -2  m 2 /s 3 treatments did not considerably affect the algal density profile compared to that without SPM, of which unicellular algae decreased and colonial algae dominated the phytoplankton community. On the other hand, the phytoplankton can regulate the SPM properties. During the 18 days' coincubation, extracellular polymeric substances (EPS) released from algal cells induced larger particle sizes and round surfaces of SPM, which can reduce the damage received to algal cells. Here we demonstrated that the phytoplankton communities could actively counteract the effects of turbulence + SPM and adapt the couple stress, jointly through the release of EPS, the modification of SPM surface properties and the conversion of their assemblage pattern, thereby contributing to rebalance the ecosystem. These findings highlight the strategies employed during the reconstruction of phytoplankton under the dual effects of turbulence and SPM for the first time, consequently enabling the forecasting of the dominant species of phytoplankton in turbulent waters., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Although stratification in deep lakes is well-discussed, few studies pay attention to thermal structure as well as its influences on stratification of biochemical parameters in subtropical lakes in mountainous cities. Here, we studied the depth profile of temperature and biochemical parameters in Longjing Lake, a subtropical reservoir in a mountainous city. Thermal stratification became strong during summer. Biochemical parameters were strongly associated with thermal structure. Stratification started at 2~6 m depth with a substantial decrease in dissolved oxygen, biochemical oxygen demand (BOD 5 ), chlorophyll a , and pH, corresponding to an increase in total nitrogen, ammonium (), nitrite (), total inorganic nitrogen (TIN), total phosphorus, and soluble reactive phosphorus (SRP) with depth; the majority of biochemical parameters showed slight variations from 12 m downward. Our results indicated the stratification of Longjing Lake was stronger and more stable than the stratification of tropical and temperate lakes in lowland cities.

Co-digestion of residual sludge (RS) and different lignocellulosic wastes (LWs) including greening waste (GW), decocted Chinese herbs waste (DCHW) and sugarcane bagasse waste (SCBW) was investigated in batch digester. Results show that the application of GW presented the highest specific methane yield (161 mL CH 4 /g VS added ) due to its high carbohydrate fraction and more balanced C/N ratio in co-substrate mixture. Buswell equation was applied and it is found that biodegradability index (BI) for co-digestion varied from 68.1% to 74.2% (53.0% for RS mono-digestion) depending on the lignin fractions of the LWs. Variation of pH, VFAs, alkalinity and ammonia throughout the digestion were also examined. The addition of LWs induced VFAs formation, as well as their conversion to methane. The higher microbial diversity in RS/LWs co-digestion further confirmed the positive effect of LWs addition in co-digestion., (Copyright © 2018. Published by Elsevier Ltd.)

Microplastics are widely identified in aquatic environments, but their impacts on phytoplankton have not been extensively studied. Here, the responses of Chlorella pyrenoidosa under polystyrene (PS) microplastics exposure were studied across its whole growth period, with microplastic sizes of 0.1 and 1.0 μm and 3 concentration gradients each, which covered (10 and 50 mg/L) and exceeded (100 mg/L) its environmental concentrations, respectively. PS microplastics caused dose-dependent adverse effects on Chlorella pyrenoidosa growth from the lag to the earlier logarithmic phases, but exhibited slight difference in the maximal inhibition ratio (approximately 38%) with respect to the two microplastic sizes. In addition to the reduced photosynthetic activity of Chlorella pyrenoidosa, unclear pyrenoids, distorted thylakoids and damaged cell membrane were observed, attributing to the physical damage and oxidative stress caused by microplastics. However, from the end of the logarithmic to the stationary phase, Chlorella pyrenoidosa could reduce the adverse effects of microplastics jointly through cell wall thickening, algae homo-aggregation and algae-microplastics hetero-aggregation, hence triggering an increase of algal photosynthetic activity and its growth, and cell structures turned to normal. Our study confirmed that PS microplastics can impair but then enhance algae growth, which will be helpful in understanding the ecological risks of microplastics., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

The sewers have the function of carbon removal, which has been proven. But if the effect of nitrogen removal can be enhanced at the same time of carbon removal, it can lay a foundation for the realization of "sewer's working as a reactor." This paper investigated the effects of shear stress and C/N ratio on nitrogen removal through biofilms on the sewer inner wall and nitrogen transfer. The main conclusions are as follows: (1) nitrogen could be partially removed in sewers after a series of reactions; (2) the anaerobic, anoxic, aerobic environment and some bacteria related to nitrogen metabolism, which exist in the biofilm, promote the nitrification and denitrification; (3) a total of 722 functional genes involved in nitrogen metabolism were detected in the biofilm (C/N ratio of 10, shear stress of 1.4 Pa), accounting for 0.67% of all genes, and the functional genes related to denitrification were dominant. Graphical abstract ᅟ.

Microcystins (MCs) pose potential threat for both aquatic organisms and humans, whereas their occurrence in response to hydrodynamic alterations are not clearly understood. Here, spatiotemporal variations of dissolved MC-RR and MC-LR were evaluated monthly in 2016 in the Yulin River, a tributary of the Three Gorges Reservoir (TGR). The environmental factors that linked to MCs concentration were discussed. The results revealed that MC-RR maximumly reached 3.55 μg/L, and the maximum MC-LR concentration exceeded the threshold value of 1.0 μg/L recommended by the WHO. MCs concentrations were higher during the flood season and decreased from the estuary to the upstream reach of the Yulin River. Ecological risk assessment confirmed that MC-LR had significant adverse effects on the benthonic invertebrates Potamopyrgus antipodarum. MCs content in the sediment was 1.70- to 20-fold higher than that in suspended particulate matter (SPM). The impacts of environmental factors on the MCs profile differed between flood and dry seasons and the longitudinal differences of MCs were determined by the longitudinal profile of water velocity and SPM content, which were affected by TGR operations. This study suggested that the occurrence of MCs in the Yulin River were influenced by hydrologic regime in TGR., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Sewer system is an important source of methane formation and emission. Although some models were developed to predict methane production in sewers, the impact of microorganism amount was indicated indirectly. Here, seven laboratory scale sewers with varied wall-shear stresses were established. The biofilm thickness, microorganism amount, DO distribution, microorganism community in the biofilms and methane production in the sewers were measured. Based on experimental data, an empirical model was developed to directly describe the relationship between methane production, microorganism amount and wall-shear stress. The results showed that DO concentration decreased significantly along the biofilm depth under varied wall-shear stress, and the DO reduction rate was positively related to the intensity of wall-shear stress. The dominant archaea species in mature biofilms were similar whereas the proportions showed remarkable differences. The abundance of Methanospirillum in biofilms cultured at 2.0 Pa wall-shear stress was 53.08% more than that at 1.29 Pa. The maximum methane production rate, 2.04 mg/L wastewater day, was obtained when the wall-shear stress kept at 1.45 Pa, which was 1.2-fold higher than the minimum in sewer at 0.5 Pa. The R 2 value of the established model was 0.95, the difference between the measurement and simulation was in the rage of 1.5-13.0%.

Phytoplankton density can be influenced by a wide range of factors whereas the role of suspended particulate matter (SPM) are not clear in river that annually subjected to hydrodynamics shift. Here, spatial-temporal variation of environmental parameters and phytoplankton density were studied from January 2013 to December 2014 in Yulin River, a tributary of the Three Gorges Reservoir, China. Laboratory experiments were conducted to elucidate the key parameter and interpret how it impacted phytoplankton density. SPM is negatively correlated with phytoplankton density. Despite SPM in Yulin River revealed weaker NH 3 -N, NO 3 -N and PO 4 -P adsorption capabilities in comparison to that in other aquatic ecosystems, increase of water velocity from 0.1 to 0.8 m/s led to approximately 6.8-times increase of light attenuation rate. In experiments evaluating the aggregation of Chlorella pyrenoidosa upon SPM, floc size showed 7.4 to 22% fold increase compared to the SPM or algae itself, which was due to the interaction between SPM and phytoplankton extracellular polymeric substances. Our results suggest that SPM could contribute to the variation of phytoplankton density through the integrated process including light attenuation, nutrient adsorption and algae aggregation. This is the first evaluation of the multiple processes underlying the impact of SPM on phytoplankton.

Emission of nitrous oxide (N 2 O) during biological wastewater treatment is of growing concern. This paper reports findings of the effects of carbon/nitrogen (C/N) ratio on N 2 O production rates in a laboratory-scale biological aerated filter (BAF) reactor, focusing on the biofilm during nitrification. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and microelectrode technology were utilized to evaluate the mechanisms associated with N 2 O production during wastewater treatment using BAF. Results indicated that the ability of N 2 O emission in biofilm at C/N ratio of 2 was much stronger than at C/N ratios of 5 and 8. PCR-DGGE analysis showed that the microbial community structures differed completely after the acclimatization at tested C/N ratios (i.e., 2, 5, and 8). Measurements of critical parameters including dissolved oxygen, oxidation reduction potential, NH 4 + -N, NO 3 - -N, and NO 2 - -N also demonstrated that the internal micro-environment of the biofilm benefit N 2 O production. DNA analysis showed that Proteobacteria comprised the majority of the bacteria, which might mainly result in N 2 O emission. Based on these results, C/N ratio is one of the parameters that play an important role in the N 2 O emission from the BAF reactors during nitrification.

The function of sewer as reactors must rely on the biofilm in it. In this paper, the formation, structure, oxygen transfer, and activity of the biofilm under different hydraulic conditions were studied by the microelectrode technology, oxygen uptake rate (OUR) technology, and 454 high-throughput pyrosequencing technology. Results showed that when the wall-shear stresses were 1.12, 1.29, and 1.45 Pa, the porosity of the steady-state biofilm were 69.1, 64.4, and 55.1 %, respectively. The maximum values of OUR were 0.033, 0.027, and 0.022 mg/(L*s), respectively, and the COD removal efficiency in the sewers reached 40, 35, and 32 %, respectively. The research findings had an important significance on how to improve the treatment efficiency of the sewers. Fig. a Graphical Abstract.

Measures for removal of toxic harmful algal blooms often cause lysis of algal cells and release of microcystins (MCs). In this study, Acinetobacter sp. CMDB-2 that exhibits distinct algal lysing activity and MCs degradation capability was isolated. The physiological response and morphological characteristics of toxin-producing Microcystis aeruginosa, the dynamics of intra- and extracellular MC-LR concentration were studied in an algal/bacterial cocultured system. The results demonstrated that Acinetobacter sp. CMDB-2 caused thorough decomposition of algal cells and impairment of photosynthesis within 24 h. Enhanced algal lysis and MC-LR release appeared with increasing bacterial density from 1 × 10 3 to 1 × 10 7 cells/mL; however, the MC-LR was reduced by nearly 94% within 14 h irrespective of bacterial density. Measurement of extracellular and intracellular MC-LR revealed that the toxin was decreased by 92% in bacterial cell incubated systems relative to control and bacterial cell-free filtrate systems. The results confirmed that the bacterial metabolite caused 92% lysis of Microcystis aeruginosa cells, whereas the bacterial cells were responsible for approximately 91% reduction of MC-LR. The joint efforts of the bacterium and its metabolite accomplished the sustainable removal of algae and MC-LR. This is the first report of a single bacterial strain that achieves these dual actions.

Sewer biofilms play an important role in the biotransformation of substances for methane and sulfide emission in sewer networks. The dynamic flows and the particular shear stress in sewers are the key factors determining the growth of the sewer biofilm. In this work, the development of sewer biofilm with varying shear stress is specifically investigated to gain a comprehensive understanding of the sewer biofilm dynamics. Sewer biofilms were cultivated in laboratory-scale gravity sewers under different hydraulic conditions with the corresponding shell stresses are 1.12 Pa, 1.29 Pa and 1.45 Pa, respectively. The evolution of the biofilm thickness were monitored using microelectrodes, and the variation in total solids (TS) and extracellular polymer substance (EPS) levels in the biofilm were also measured. The results showed that the steady-state biofilm thickness were highly related to the corresponding shear stresses with the biofilm thickness of 2.4 ± 0.1 mm, 2.7 ± 0.1 mm and 2.2 ± 0.1 mm at shear stresses of 1.12 Pa, 1.29 Pa and 1.45 Pa, respectively, which the chemical oxygen demand concentration is 400 mg/L approximately. Based on these observations, a kinetic model for describing the development of sewer biofilms was developed and demonstrated to be capable of reproducing all the experimental data.

The hydrogen ion production rate (HPR) and the pH of the aeration phase in a sequencing batch reactor (SBR) were simultaneously measured by a novel respirometric-titrimetric instrument. The results showed that HPR could indicate the end of ammonia oxidation with a greater accuracy and sensitivity than pH. An SBR was used to treat synthetic wastewater containing 360 mg/L chemical oxygen demand (COD) and 40 mg/L NH(4+)-N at 20 degrees C with dissolved oxygen (DO) lower than 2.0 mg/L. Controlling the aeration duration based on HPR online monitoring, shortcut nitrification-denitrification was successfully performed for approximately two months with a stable nitrite accumulation rate (NAR) above 88%, and the COD and NH(4+)-N removal ratios were both higher than 90%. Based on the HPR online monitoring data, the estimated NH(4+)-N concentrations in nitrification were closely related to the measured concentrations, with a correlation coefficient of 0.9722, and the estimated values were lower than the measured values mainly because of the titration delay at the beginning of the aeration phase.

To investigate the distribution of pollutant concentrations and pollution loads in stormwater runoff in Chongqing, six typical land use types were selected and studied from August 2009 to September 2011. Statistical analysis on the distribution of pollutant concentrations in all water samples shows that pollutant concentrations fluctuate greatly in rainfall-runoff, and the concentrations of the same pollutant also vary greatly in different rainfall events. In addition, it indicates that the event mean concentrations (EMCs) of total suspended solids (TSS) and chemical oxygen demand (COD) from urban traffic roads (UTR) are significantly higher than those from residential roads (RR), commercial areas (CA), concrete roofs (CR), tile roofs (TRoof), and campus catchment areas (CCA); and the EMCs of total phosphorus (TP) and NH3-N from UTR and CA are 2.35-5 and 3 times of the class-II standard values specified in the Environmental Quality Standards for Surface Water (GB 3838-2002). The EMCs of Fe, Pb and Cd are also much higher than the class-III standard values. The analysis of pollution load producing coefficients (PLPC) reveals that the main pollution source of TSS, COD and TP is UTR. The analysis of correlations between rainfall factors and EMCs/PLPC indicates that rainfall duration is correlated with EMCs/PLPC of TSS for TRoof and TP for UTR, while rainfall intensity is correlated with EMCs/PLPC of TP for both CR and CCA. The results of this study provide a reference for better management of non-point source pollution in urban regions.

An integrated process consisting of an anaerobic/anoxic expanded granular sludge bed (EGSB) reactor and an aerobic sequencing batch reactor (SBR) was developed by a mode of sequencing batch operation, in which methanogenesis, denitrification and anammox were coupled in EGSB with methanogenesis first, then denitrification and anammox simultaneously, and partial nitrification occurred in SBR for providing nitrite to EGSB. This process extended the application of the anammox process to the treatment of wastewater containing high concentrations of chemical oxygen demand (COD) and ammonium. When the volumetric exchange ratio between EGSB and SBR was controlled at 57% with the influent pH at 6-8, 74.38-83.65% of NH(4)(+)-N, 72.68-83.12% of total nitrogen (TN) and 88.34-98.86% of COD were removed in a range of 200-4,500 mg/L COD and 40-90 mg/L NH(4)(+)-N respectively. TN removal by anammox and shortcut denitrification was 26.35-58.64 and 0-32.80% of the removed nitrogen, respectively. The results showed that the contribution of anammox gradually decreased with an increase in the C/N ratio of influent, whereas the reverse was true for shortcut denitrification. The COD removal by methanogenesis was 70.89-98.79% of the removed COD, and increased with increasing C/N ratio.

Characterization of volatile fatty acid (VFA) in wastewater is significant for understanding the wastewater nature and the wastewater treatment process optimization based on the usage of Activated Sludge Models (ASMs). In this study, a nine-point pH titration method was developed for the determination of low-concentration VFA in municipal wastewater. The method was evaluated using synthetic wastewater containing VFA with the concentration of 10-50 mg/l and the possible interfering buffer systems of carbonate, phosphate and ammonium similar to those in real municipal wastewater. In addition, the further evaluation was conducted through the assay of real wastewater using chromatography as reference. The results showed that the recovery of VFA in the synthetic wastewater was 92%-102 and the coefficient of variance (CV) of reduplicate measurements 1.68%-4.72%. The changing content of the buffering substances had little effect on the accuracy of the method. Moreover, the titration method was agreed with chromatography in the determination of VFA in real municipal wastewater with R(2)= 0.9987 and CV =1.3-1.7. The nine-point pH titration method is capable of satisfied determination of low-concentration VFA in municipal wastewater.