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The increasing occurrence of micropollutants in natural water bodies has medium to long-term effects on both aquatic life and human health. The aim of this study is to optimize the degradation of two pharmaceutical pollutants of emerging concern: amoxicillin and acetaminophen in aqueous solution at laboratory and pilot scale, by solar photo-Fenton process carried out at neutral pH using ethylenediamine-N,N'-disuccinic acid (EDDS) as a complexing agent to maintain iron in solution. The initial concentration of each compound was set at 1 mg/L dissolved in a simulated effluent from a municipal wastewater treatment plant (MWTP). A factorial experimental design and its surface response analysis were used to optimize the operating parameters to achieve the highest initial degradation rate of each target. The evolution of the degradation process was measured by ultra-performance liquid chromatography (UPLC/UV), obtaining elimination rates above 90% for both contaminants. Statistical study showed the optimum concentrations of Fe(III) at 3 mg/L at an Fe-EDDS ratio of 1:2 and 2.75 mg/L H 2 O 2 for the almost complete removal of the target compounds by solar photo-Fenton process. Validation of the experimental design was successfully carried out with actual MWTP effluent spiked with 100 μg/L of amoxicillin and acetaminophen, each at pilot plant scale., (© 2023. The Author(s).)

Harmful algal blooms (HABs) in coastal areas similarly impact both ecosystems and human health. The translocation of phytoplankton species via maritime transport can potentially promote the growth of HABs in coastal systems. Accordingly, ballast water must be disinfected. The main goal of this study is to assess the effectiveness of different emerging biocides, including H 2 O 2 , peracetic acid (PAA), peroxymonosulfate (PMS), and peroxydisulfate (PDS). The effectiveness of these biocides is compared with that of conventional chlorination methods. Their effects on two ichthyotoxic microalgae with worldwide distribution, i.e., Prymnesium parvum and Heterosigma akashiwo, are examined. To ensure the prolonged effectiveness of the different reagents, their concentration-response curves for 14 days are constructed and examined. The results suggest a strong but shorter effect by PMS (EC50 = 0.40-1.99 mg·L -1 ) and PAA (EC50 = 0.32-2.70 mg·L -1 ), a maintained effect by H 2 O 2 (EC50 = 6.67-7.08 mg·L -1 ), and a negligible effect by PDS. H. akashiwo indicates higher resistance than P. parvum, except when H 2 O 2 is used. Based on the growth inhibition performance and consumption of the reagents as well as a review of important aspects regarding their application, using H 2 O 2 , PAA, or PMS can be a feasible alternative to chlorine-based reagents for inhibiting the growth of harmful phytoplankton., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)

Ultraviolet (UV) radiation is a well-implemented process for water disinfection. The development of emergent UV sources, such as light-emitting diodes (LEDs), has afforded new possibilities for advanced oxidation processes. The emission wavelength is considered to be an important factor for photo-chemical processes in terms of both biological damage and energetic efficiency, as the inactivation mechanisms and mode-of-action may differ according to the wavelength that is applied. In addition, these processes merit exploration for inactivating emerging pathogens, such as marine vibrios, that are important bacteria to control in maritime activities. The main goal of this study was to compare the disinfection efficacy of several UV-LED driven processes with different modes of action. First, the effect of UV-LEDs was assessed at different UV ranges (UV-A, UV-B, or UV-C). Second, the possible enhancement of a combination with hydrogen peroxide (H 2 O 2 ) or peroxymonosulfate salt (HSO 5 ) was investigated under two different application strategies, i.e. simultaneous or sequential. The results obtained indicate a high sensitivity of Vibrio alginolyticus to UV radiation, especially under UV-B (k - ) was investigated under two different application strategies, i.e. simultaneous or sequential. The results obtained indicate a high sensitivity of Vibrio alginolyticus to UV radiation, especially under UV-B (k obs  = 0.24 cm 2 /mJ) and UV-C (k obs  = 1.47 cm 2 /mJ) irradiation. The highest inactivation rate constants were obtained for UV/HSO 5 - /mJ)=0.0007 (UV-A); 0.39 (UV-B); 1.79 (UV-C)) with respect to UV/H obs (cm 2 /mJ)=0.0007 (UV-A); 0.39 (UV-B); 1.79 (UV-C)) with respect to UV/H 2 O 2 /mJ)=0.0006 (UV-A); 0.26 (UV-B); and 1.54 (UV-C)) processes, however, regrowth was avoided only with UV/H obs (cm 2 . Additionally, the disinfection enhancement caused by a chemical addition was more evident in the order UV-A > UV-B > UV-C. By applying H 2 O 2 . Additionally, the disinfection enhancement caused by a chemical addition was more evident in the order UV-A > UV-B > UV-C. By applying H 2 (2.5 mg/L) in a sequential mode before the UV, negligible effects were obtained in comparison with the simultaneous application. Finally, promising electrical energy per order (EE 2 (10 mg/L) or HSO 5 - (2.5 mg/L) in a sequential mode before the UV, negligible effects were obtained in comparison with the simultaneous application. Finally, promising electrical energy per order (EE O )=1.68 (UV-A); 0.20 (UV-B); 0.04 (UV-C)) and UV/H 5 - (EE O (kWh/m 3 )=2.15 (UV-A); 0.32 (UV-B); 0.04 (UV-C)), demonstrating the potential of UV-LEDs for disinfection in particular activities such as the aquaculture industry or maritime transport.2 O 2 (EE O (kWh/m 3 )=2.15 (UV-A); 0.32 (UV-B); 0.04 (UV-C)), demonstrating the potential of UV-LEDs for disinfection in particular activities such as the aquaculture industry or maritime transport., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)

The Ballast Water Management Convention (BWMC) establishes limits regarding the permissible number of viable organisms in discharged ballast water. Ozone as a ballast water treatment is interesting because it can be generated in-situ and has strong oxidant power. Additionally, some oxidants can be formed in reaction with seawater, especially brominated compounds, that assist in inactivating microorganisms. The objective of this study is to assess the efficacy of semicontinuous and batch ozonation as well as their combination with peroxymonosulfate salt (PMS) as methods to be used to ensure compliance with regulation D2 of the BWMC using Tetraselmis suecica as a standard microorganism. Growth modeling method was employed to determine the inactivation achieved by the treatments. The results show that ozone is an effective treatment for accomplishing the D2 of the BWMC. Batch ozonation is more efficient than semicontinuous ozonation probably because of the brominated compounds formed during the ozone saturation of the water. The oxidants that are developed during the ozonation of seawater prolong the residual effect of the treatment throughout the days of storage with practically no presence of them in the ballast tanks at 72 h. The addition of the PMS increases the inactivation in the semicontinuous ozonation, but a threshold concentration of ozone is needed to observe the synergistic effect of both oxidants. No increase is associated with the combination of O 3 and PMS in the case of batch ozonation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)

Harmful algal blooms in coastal areas can significantly impact a water source. Microorganisms such as cyanobacteria and associated pathogenic bacteria may endanger an ecosystem and human health by causing significant eco-hazards. This study assesses the efficacy of two different reagents, H2O2 and S2O82−, as (pre-)treatment options for algae-laden waters. Anabaena sp. and Vibrio alginolyticus have been selected as target microorganisms. With the objective of activating H2O2 or S2O82−, additional experiments have been performed with the presence of small amounts of iron (18 µmol/L). For the cyanobacterial case, H2O2-based processes demonstrate greater efficiency over that of S2O82−, as Anabaena sp. is particularly affected by H2O2, for which >90% of growth inhibition has been achieved with 0.088 mmol/L of H2O2 (at 72 h of exposure). The response of Anabaena sp. as a co-culture with V. alginolyticus implies the use of major H2O2 amounts for its inactivation (0.29 mmol/L of H2O2), while the effects of H2O2/Fe(II) suggests an improvement of ~60% compared to single H2O2. These H2O2 doses are not sufficient for preventing the regrowth of V. alginolyticus after 24 h. The effects of S2O82− (+ Fe(II)) are moderate, reaching maximum inhibition growth of ~50% for Anabaena sp. at seven days of exposure. Nevertheless, doses of 3 mmol/L of S2O82− can prevent the regrowth of V. alginolyticus. These findings have implications for the mitigation of HABs but also for the associated bacteria that threaten many coastal ecosystems.

The Ballast Water Management Convention (BWMC) establishes limits for viable organisms in discharged ballast water. However, organisms smaller than 10 μm are not considered in this regulation although they represent, in some regions, the majority of the phytoplankton organisms in marine water. The objective in this study is to assess three photosynthetic species smaller than 10 μm as potential standard test organism (STO) in experimentation focused on the inactivating efficacy of ultraviolet treatments (UV). A growth modelling method was employed to determine the reduction of the viable cell concentration under either light or dark post-treatment conditions to evaluate the importance of the photoreactivation. In spite of its moderate growth rate, the high UV resistance in combination with the abundance and worldwide distribution of Synechococcus sp. and the environmental importance of this species constitute important reasons for considering Synechococcus sp. as a valuable STO for ballast water treatment., (Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Due to the increasing number of ecosystem invasions with the introduction of exogenous species via ballast water, the International Maritime Organization adopted the Ballast Water Convention (BWMC). The BWMC establishes standards for the concentration of viable organisms in a ballast water discharge. Ultraviolet (UV) irradiation is commonly used for treating ballast water; however, regrowth after UV irradiation and other drawbacks have been reported. In this study, improvement in UV treatment with the addition of hydrogen peroxide or peroxymonosulfate salt was investigated using the microalgae Tetraselmis suecica as the target organism. Results reported that each of these reagents added in a concentration of 10 ppm reduced the concentration of initial cells by more than 96%, increased the UV inactivation rate, and enabled reaching greater level of inactivation with the treatment. These improvements imply a reduction of the UV doses required for a consistent compliance with the BWMC standards., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

The development of technologically advanced recirculation aquaculture systems (RAS) implies the reuse of water in a high recirculation rate (>90%). One of the most important phases for water management in RAS involves water disinfection in order to avoid proliferation of potential pathogens and related fish diseases. Accordingly, different approaches have been assessed in this study by performing a comparison of photolytic (UV-LEDs) at different wavelengths (λ = 262, 268 and 262 + 268 nm), photochemical (UV-LEDs/H 2 O 2 , UV-LEDs/HSO 5 - and UV-LEDs/S 2 O 8 2- ) and photocatalytic (TiO 2 /SiO 2 in front of single wavelengths. Photochemical processes showed higher efficiencies by comparison with a single UV-C process, especially at 10 mg L 2 /UV-LEDs) processes for the disinfection of water in RAS streams. Different laboratory tests were performed in batch scale with real RAS stream water and naturally occurring bacteria (Aeromonas hydrophyla and Citrobacter gillenii) as target microorganisms. Regarding photolytic processes, higher inactivation rates were obtained by combining λ 262+268 in front of single wavelengths. Photochemical processes showed higher efficiencies by comparison with a single UV-C process, especially at 10 mg L -1 of initial oxidant dose. The inactivation kinetic rate constant was improved in the range of 15-38%, with major efficiency for UV/H 2 O 2  ∼ UV/HSO 5 . According to photocatalytic tests, higher efficiencies were obtained by improving the inactivation kinetic rate constant up to 55% in comparison with a single UV-C process. Preliminary cost estimation was conducted for all tested disinfection methods. Those results suggest the potential application of UV-LEDs as promoter of different photochemical and photocatalytic processes, which are able to enhance disinfection in particular cases, such as the aquaculture industry.-  > UV/S 2 O 8 2- . According to photocatalytic tests, higher efficiencies were obtained by improving the inactivation kinetic rate constant up to 55% in comparison with a single UV-C process. Preliminary cost estimation was conducted for all tested disinfection methods. Those results suggest the potential application of UV-LEDs as promoter of different photochemical and photocatalytic processes, which are able to enhance disinfection in particular cases, such as the aquaculture industry., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Meeting the recent biological standards established by the Ballast Water Management Convention requires the application of ballast water treatment systems; ultraviolet irradiation is a frequently used option. However, organisms can repair the damage caused by ultraviolet irradiation primarily with photo-repair mechanisms that are dependent on the availability of light. The objective of this study is to quantify the impact of dark storage following ultraviolet irradiation on the viability of the microalgae Tetraselmis suecica. Results showed that one day of dark storage after ultraviolet irradiation enhanced the inactivation rate by 50% with respect to the absence of dark storage and increased up to the 84% with five days of dark storage. These results are consistent with photorepair, mostly in the first two days, prevented in the dark. The dose required to inactivate a determined ratio of organisms was correlated with the length of the dark post-treatment according to an inverse proportional function. This correlation may help to optimize the operation of ultraviolet ballast water treatment systems. Further, the results show that growth assays can detect organisms that are capable of repair after treatment with UV., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Over the years, industrial activities that generate high salinity effluents have been intensifying; this has relevant potential for causing organic and microbiological pollution which damages both human and ocean health. The development of new regulations, such as ballast water convention, encourage the development of treatment systems that can be feasible for treating seawater effluents. Accordingly, an approach based on the UV activation of persulfate salts has been assessed. In this scenario, two different persulfate sources (S 2 O 8 2- ) were evaluated under UV-C irradiation for disinfection purposes. An optimization process was performed with low chemical doses (<1 mM). In order to extensively examine the applicability on seawater, different water matrices were tested as well as different microorganisms including both fecal and marine bacteria. An enhancement of UV-inactivation with the addition of persulfate salts was achieved in all cases, kinetic rate constant has been accelerated by up to 79% in seawater. It implies a UV-dose saving up to 45% to achieve 4-log reductions. Best efficiencies were obtained with [HSO 5 - ) were evaluated under UV-C irradiation for disinfection purposes. An optimization process was performed with low chemical doses (<1 mM). In order to extensively examine the applicability on seawater, different water matrices were tested as well as different microorganisms including both fecal and marine bacteria. An enhancement of UV-inactivation with the addition of persulfate salts was achieved in all cases, kinetic rate constant has been accelerated by up to 79% in seawater. It implies a UV-dose saving up to 45% to achieve 4-log reductions. Best efficiencies were obtained with [HSO 5 - due to its low stability and interaction with chloride. Also, different responses were obtained according to the specific microorganisms by achieving faster disinfection in Gram-negative than in Gram-positive bacteria, the sensitivity observed was Vibrio spp. > E. coli > E. faecalis ≈ Marine Heterotrophic Bacteria. With an evaluation of regrowth after treatment, greater cell damage was detected with the addition of persulfate salts. The major ability of regrowth for marine bacteria encourages the use of a residual disinfectant after disinfection processes.2 O 8 2- ] = 0.5 mM. Higher effectiveness was obtained with the use of HSO 5 - due to its low stability and interaction with chloride. Also, different responses were obtained according to the specific microorganisms by achieving faster disinfection in Gram-negative than in Gram-positive bacteria, the sensitivity observed was Vibrio spp. > E. coli > E. faecalis ≈ Marine Heterotrophic Bacteria. With an evaluation of regrowth after treatment, greater cell damage was detected with the addition of persulfate salts. The major ability of regrowth for marine bacteria encourages the use of a residual disinfectant after disinfection processes., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

The present study shows the results of solar photo-Fenton oxidation of paracetamol (PCT) and amoxicillin (AMX). Fe 2 (SO 4 ) 3 was used as the source of iron and EDDS as the iron complexing agent, employing different doses of hydrogen peroxide. Two aqueous matrices, a synthetic wastewater and real wastewater from El Ejido WWTP effluent (Almeria) were used. In all cases, the process was operated under conditions of natural sunlight. Results showed that the degradation of both drugs is favoured when the aqueous matrix presents low concentration of carbonates. Under the conditions studied here, degradation percentages above 90% were obtained in the synthetic wastewater and 80% in the actual effluent. The degradation products were determined using liquid chromatography coupled to high-resolution mass spectrometry with hybrid quadrupole time-of-flight analyser. The intermediates detected throughout the oxidative process for both micro-contaminants were mainly products of hydroxylation reactions. The toxicity of the samples was determined using the bacterium Vibrio fischeri. In the acute toxicity test, it was observed that the bacteria did not undergo inhibition in any of the cases. However, chronic toxicity studies showed that the higher the Hydraulic Retention Time (HRT) employed in the assays, the higher the bacterial inhibition., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

The development of advanced photochemical processes has experienced the emergence of a promising alternative for water disinfection, different from traditional methods. The applicability has primarily been investigated in drinking and wastewater; however, new challenges related to microbiological control in marine waters necessitate evaluating the applicability of this process in such water matrices. In this study, the efficacy of persulfate (PDS) activated with UV-light against E. faecalis has been tested on the bench scale. Firstly, optimization of the different PDS concentrations (1-10 mM) and exposure times (0-5 min) was performed in distilled water. 1 mM of PDS was selected as the best dosage within the range tested. Secondly, in order to evaluate the effects of different inorganic compounds usually found in natural waters, the efficiency of the UV/PDS system was tested in three different matrices: mineral water, saltwater, and marine saltwater. Finally, different bacteria were evaluated in consortium (E. coli + E. faecalis), suggesting the same inactivation level independently on the bacterial groups and structures. The results suggest that PDS is an attractive alternative to other photochemical processes currently in use for seawater treatment and this application deserved further research.

Cruise ship wastewater discharges are pollution sources towards the marine environment that are poorly characterized. In this study, wastewater samples from cruise ships have been obtained during repair works in a shipyard. Different organic pollutants have been analyzed and their concentrations were similar to those in urban wastewaters for pharmaceuticals and fragrances, but higher for UV filters and PAHs. For the first time, cypermethrin, a pesticide highly toxic towards aquatic species, was found at relevant concentrations (>1 µg L^-1). The faecal microorganisms were for all parameters higher than 10^4 CFU 100 mL^ - 1, which together with the presence of antibiotic compounds in wastewater (e.g., triclosan), could potentially lead to the generation of antibiotic resistance bacteria (ARB). The historical position of cruise ships, determined from the Automatic Identification System (AIS), were used to define the time ships were underway, at port, or in repair. From ship's passenger and crew load, and from estimates of discharges the total volume of wastewater produced by these ships (371,000 m^3 year^-1) and the average flow (0.15 ± 0.03 m^3crew^-1 day^-1) were calculated., (Copyright © 2018 Elsevier Inc. All rights reserved.)

The importance of seawater treatment in order to avoid microbiological pollution related to aquaculture or ballast water management has increased during the last few years. Bacterial indicators used for the evaluation of different disinfection treatments are usually related with both waste and drinking water, these standards are not usual microorganisms found in seawater. Thus, it is thought necessary to study the behavior of different marine-specific organisms in regard to improve the disinfection processes in seawater. In this study, three different bacteria have been selected among major groups of bacterial community from marine waters: two water-associated, Roseobacter sp. and Pseudomonas litoralis, and one sediment-associated, Kocuria rhizophila. A kinetic inactivation model together with a post-treatment growth tendency has been obtained after the application of UV-C and UV/H 2 O 2 processes. According to the first kinetic rate constant, different responses were obtained for the different bacterial groups. Once the treatment was applied, modeling of growth curves revealed high recover within the first 3 days after treatment, even when UV/H 2 O 2 was applied. This study introduces a sensitivity index, in which results show different levels of resistance for both treatments, being Roseobacter sp. the most sensitive bacteria, followed by P. litoralis and K. rhizophila.

Seawater treatment is increasingly required due to industrial activities that use substantial volumes of seawater in their processes. The shipping industry and the associated management of a ship's ballast water are currently considered a global challenge for the seas. Related to that, the suitability of an Electrochemical Advanced Oxidation Process (EAOP) with Boron Doped Diamond (BDD) electrodes has been assessed on a laboratory scale for the disinfection of seawater. This technology can produce both reactive oxygen species and chlorine species (especially in seawater) that are responsible for inactivation. The EAOP was applied in a continuous-flow regime with real seawater. Natural marine heterotrophic bacteria (MHB) were used as an indicator of disinfection efficiency. A biphasic inactivation kinetic model was fitted on experimental points, achieving 4-Log reductions at 0.019 Ah L -1 . By assessing regrowth after treatment, results suggest that higher bacterial damages result from the EAOP when it is compared to chlorination. Furthermore, several issues lacking fundamental understanding were investigated such as recolonization capacity or bacterial community dynamics. It was concluded that, despite disinfection processes being effective, there is not only a possibility for regrowth after treatment but also a change on bacterial population diversity produced by the treatment. Finally, energy consumption was estimated and indicated that 0.264 kWh·m -3 are needed for 4.8-Log reductions of MHB; otherwise, with 0.035 kWh·m -3 , less disinfection efficiency can be obtained (2.2-Log red). However, with a residual oxidant in the solution, total inactivation can be achieved in three days., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Assessing the disinfection of ballast water and its compliance with international standards requires determining the size, viability, and concentration of planktonic organisms. The FlowCAM (Flow Cytometer and Microscope) is an Imaging Flow Cytometry designed to obtain the particle concentration, images, and quantitative morphologic information. The objective in this paper is to establish the basis for transforming the FlowCAM from being a laboratory analyzer into a tool for systematic monitoring of ballast water. The capacity of the FlowCAM was evaluated by analyzing artificial microbeads, phytoplankton monocultures, and real seawater samples. Microbead analyses reported high accuracy and precision in size and concentration measurements. Monoculture analyses showed the effect of disinfection treatments in cell appearance and growth. Low concentration and heterogeneity of particles in real seawater analyses require the comprehensive observation of images by experts. Additionally, some physical characteristics of the device must be improved. The optimization of device configuration enables the quick transferring of files and information between parties involved in ballast water management. FlowCAM may become a feasible technology for this after the device and protocols are adapted., (Copyright © 2017 Elsevier B.V. All rights reserved.)

Water contained on ships is employed in the majority of activities on a vessel; therefore, it is necessary to correctly manage through marine water treatments. Among the main water streams generated on vessels, ballast water appears to be an emerging global challenge (especially on cargo ships) due to the transport of invasive species and the significant impact that the ballast water discharge could have on ecosystems and human activities. To avoid this problem, ballast water treatment must be implemented prior to water discharge in accordance with the upcoming Ballast Water Management Convention. Different UV-based treatments (photolytic: UV-C and UV/H 2 O 2 , photocatalytic: UV/TiO 2 ), have been compared for seawater disinfection. E. faecalis is proposed as a biodosimeter organism for UV-based treatments and demonstrates good properties for being considered as a Standard Test Organism for seawater. Inactivation rates by means of the UV-based treatments were obtained using a flow-through UV-reactor. Based on the two variables responses that were studied (kinetic rate constant and UV-Dose reductions), both advanced oxidation processes (UV/H 2 O 2 and photocatalysis) were more effective than UV-C treatment. Evaluation of salinity on the processes suggests different responses according to the treatments: major interference on photocatalysis treatment and minimal impact on UV/H 2 O 2 ., (Copyright © 2016 Elsevier B.V. All rights reserved.)

The main aim of this work was to study the feasibility of multi-barrier treatment (MBT) consisting of filtration, hydrogen peroxide photolysis (H2O2/UVC) and catalytic wet peroxide oxidation (CWPO) for post-treatment of petroleum refinery effluent. Also the possibility of water reuse or safe discharge was considered. The performance of MBT using medium (MP) and low (LP) pressure lamps was compared as well as operation and maintenance (O&M) cost. Decomposition of organic compounds was followed by means of gas chromatography-mass spectrometry (GC-MS), total organic carbon (TOC) and chemical oxygen demand (COD) analysis. After filtration step (25 μm) turbidity and concentration of suspended solids decreased by 92% and 80%, respectively. During H2O2/UVC process with LP lamp at optimal conditions (H2O2:TOC ratio 8 and UVC dose received by water 5.28 WUVC s cm(-2)) removal of phenolic compounds, TOC and COD was 100%, 52.3% and 84.3%, respectively. Complete elimination of phenolic compounds, 47.6% of TOC and 91% of COD was achieved during H2O2/UVC process with MP lamp at optimal conditions (H2O2:TOC ratio 5, UVC dose received by water 6.57 WUVC s cm(-2)). In order to compare performance of H2O2/UVC treatment with different experimental set up, the UVC dose required for removal of mg L(-1) of COD was suggested as a parameter and successfully applied. The hydrophilicity of H2O2/UVC effluent significantly increased which in turn enhanced the oxidation of organic compounds during CWPO step. After H2O2/UVC treatment with LP and MP lamps residual H2O2 concentration was 160 mg L(-1) and 96.5 mg L(-1), respectively. Remaining H2O2 was fully consumed during subsequent CWPO step (6 and 3.5 min of contact time for LP and MP, respectively). Total TOC and COD removal after MBT was 94.7% and 92.2% (using LP lamp) and 89.6% and 95%, (using MP lamp), respectively. The O&M cost for MBT with LP lamp was estimated to be 0.44 € m(-3) while with MP lamp it was nearly five times higher. Toxicity assessment was performed using two marine species (Vibrio fischeri and Paracentrotus lividus sea-urchin) after each treatment step. The highest toxicity was attributed to H2O2/UVC effluent for both tested species. After MBT a drastic decrease of toxicity was achieved., (Copyright © 2016 Elsevier Ltd. All rights reserved.)

The aim of this study has been the identification of the nutritive status (oligotrophy end eutrophy) in four enclosure areas from Gulf of Cadiz (Southwest Iberian Peninsula-Europe) by means of a battery of water column toxicity tests. The test organisms were four marine microalgae which comprised a wide taxonomic range. Growth tests with receiving waters from the four study areas were carried out under two nutritive conditions; with and without additional nutrient-enriched medium. The results of both bioassays were used to define a new eutrophication index (I(E)). The I(E) index obtained presents a good correlation with the traditional physical-chemical parameters in the enclosure areas; nevertheless in open waters where the dilution processes are more important the results show less correlation.