1. Dental pulp stem cells ameliorate D-galactose-induced cardiac ageing in rats.
- Author
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El-Akabawy G, El-Kersh SOF, El-Kersh AOFO, Amin SN, Rashed LA, Abdel Latif N, Elshamey A, Abdallah MAAEM, Saleh IG, Hein ZM, El-Serafi I, and Eid N
- Subjects
- Animals, Male, Rats, Stem Cell Transplantation methods, Aging physiology, Sirtuin 1 metabolism, Cell Differentiation drug effects, Connexin 43 metabolism, Disease Models, Animal, Stem Cells metabolism, Stem Cells cytology, Apoptosis drug effects, Rats, Sprague-Dawley, Galactose, Myocytes, Cardiac metabolism, Myocytes, Cardiac transplantation, Myocytes, Cardiac drug effects, Dental Pulp cytology
- Abstract
Background: Ageing is a key risk factor for cardiovascular disease and is linked to several alterations in cardiac structure and function, including left ventricular hypertrophy and increased cardiomyocyte volume, as well as a decline in the number of cardiomyocytes and ventricular dysfunction, emphasizing the pathological impacts of cardiomyocyte ageing. Dental pulp stem cells (DPSCs) are promising as a cellular therapeutic source due to their minimally invasive surgical approach and remarkable proliferative ability., Aim: This study is the first to investigate the outcomes of the systemic transplantation of DPSCs in a D-galactose (D-gal)-induced rat model of cardiac ageing. Methods. Thirty 9-week-old Sprague-Dawley male rats were randomly assigned into three groups: control, ageing (D-gal), and transplanted groups (D-gal + DPSCs). D-gal (300 mg/kg/day) was administered intraperitoneally daily for 8 weeks. The rats in the transplantation group were intravenously injected with DPSCs at a dose of 1 × 10
6 once every 2 weeks., Results: The transplanted cells migrated to the heart, differentiated into cardiomyocytes, improved cardiac function, upregulated Sirt1 expression, exerted antioxidative effects, modulated connexin-43 expression, attenuated cardiac histopathological alterations, and had anti-senescent and anti-apoptotic effects., Conclusion: Our results reveal the beneficial effects of DPSC transplantation in a cardiac ageing rat model, suggesting their potential as a viable cell therapy for ageing hearts., Competing Interests: The authors declare there are no competing interests., (©2024 El-Akabawy et al.)- Published
- 2024
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