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Due to the common contamination of multiple mycotoxins in food, which results in stronger toxicity, it is particularly important to simultaneously test for various mycotoxins for the protection of human health. In this study, a disposable immunosensor array with low-cost was designed and fabricated using cellulose paper, polydimethylsiloxane (PDMS), and semiconducting single-walled carbon nanotubes (s-SWCNTs), which was modified with specific antibodies for mycotoxins AFB1 and FB1 detection. The strategy for fabricating the immunosensor array with two individual channels involved a two-step protocol starting with the form of two kinds of carbon films by depositing single-wall carbon nanotubes (SWCNTs) and s-SWCNTs on the cellulose paper as the conductive wire and sensing element, followed by the assembly of chemiresistive biosensor with SWCNTs strip as the wire and s-SWCNTs as the sensing element. After immobilizing AFB1-bovine serum albumin (AFB1-BSA) and FB1-bovine serum albumin (FB1-BSA) separately on the different sensing regions, the formation of mycotoxin-BSA-antibody immunocomplexes transfers to electrochemical signal, which would change with the different concentrations of free mycotoxins. Under optimal conditions, the immunosensor array achieved a limit of detection (LOD) of 0.46 pg/mL for AFB1 and 0.34 pg/mL for FB1 within a wide dynamic range from 1 pg/mL to 20 ng/mL. Furthermore, the AFB1 and FB1 spiked in the ground corn and wheat extracts were detected with satisfactory recoveries, demonstrating the excellent practicality of this established method for simultaneous detection of mycotoxins., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Background: Accurate real-time eye tracking is crucial in oculomotor system research. While the scleral search coil system is the gold standard, its implantation procedure and bulkiness pose challenges. Camera-based systems are affected by ambient lighting and require high computational and electric power., New Method: This study presents a novel eye tracker using proximity capacitive sensors made of carbon-nanotube-paper-composite (CPC). These sensors detect femtofarad-level capacitance changes caused by primate corneal movement during horizontal and vertical eye rotations. Data processing and machine learning algorithms are evaluated to enhance the accuracy of gaze angle prediction., Results: The system performance is benchmarked against the scleral coil during smooth pursuits, saccades tracking, and fixations. The eye tracker demonstrates up to 0.97 correlation with the coil in eye tracking and is capable of estimating gaze angle with a median absolute error as low as 0.30°., Comparison: The capacitive eye tracker demonstrates good consistency and accuracy in comparison to the gold-standard scleral search coil method., Conclusions: This lightweight, non-invasive capacitive eye tracker offers potential as an alternative to traditional coil and camera-based systems in oculomotor research and vision science., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper, (Copyright © 2024. Published by Elsevier B.V.)

This study prepared a novel, portable and cost-effective microfluidic paper-based electrochemical analysis device (μ-PAD) using black phosphorus nanosheets@carboxylated multi-walled carbon nanotubes (BPNSs@MWCNTs-COOH) nanocomposites for β-lactoglobulin (β-LG) detection. At the appreciate ratio, the synthesized BPNSs@MWCNTs-COOH was demonstrated to not only serve as a high-quality substrate for the specific aptamer immobilization, but also improve the electron transfer capability of the sensing interface. The μ-PADs, utilizing BPNSs@MWCNTs-COOH and aptamer recognition, exhibited a wider detection range (10-1000 ng mL -1 ) and lower detection limit (LOD: 0.12 ng mL -1 ) for β-LG, and demonstrated enhanced specificity, satisfactory anti-interference ability and stability. When applied to the β-LG determination in dairy samples, the μ-PAD yielded β-LG concentrations highly correlated with those obtained using the HPLC method (R 2 : 0.9982). These results emphasized the reliable performance of the developed μ-PADs in β-LG allergen quantification, highlighting their potential as an efficient platform for the rapid screening of β-LG allergens., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Microfluidic paper-based analytical device (μPAD) offers a simple and efficient platform for point-of-care monitoring, which can be beneficial for copper determination in livestock feed and manure. However, common cellulose paper has excellent hydrophilicity, causing μPAD is accompanied by poor mechanical properties, short service life, and low sensitivity. Here, a self-cleaning paper-based microfluidic biosensor for Cu 2+ determination was proposed to overcome the mentioned shortages in the application. Polymeric octadecyl trichlorosilane was synthesized and decorated on cellulose paper to form hydrophobic paper, which can improve the hydrophobicity, self-cleaning, and pollution ability. In addition, hydrophobic paper, semiconducting single-walled carbon nanotube, and DNAzyme through the chemical bond were employed to fabricate a self-cleaning paper-based microfluidic biosensor. The properties were investigated using scanning electron microscopy, Raman, and electrochemical methods. The detecting parameters were also optimized. It could measure the Cu 2+ concentration from 1 nM to 100 μM, and the detection limit was 0.65 nM. The self-cleaning paper-based microfluidic biosensor was applied to detect Cu 2+ concentration in livestock feed and manure that can meet the requirements for fast screening and detection., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Skin-based wearable devices have gained significant attention due to advancements in soft materials and thin-film technologies. Nevertheless, traditional wearable electronics often entail expensive and intricate manufacturing processes and rely on metal-based substrates that are susceptible to corrosion and lack flexibility. In response to these challenges, this paper has emerged with an alternative substrate for wearable electrodes due to its cost-effectiveness and scalability in manufacturing. Paper-based electrodes offer an attractive solution with their inherent properties of high breathability, flexibility, biocompatibility, and tunability. In this study, we introduce carbon nanotube-based paper composites (CPC) electrodes designed for the continuous detection of biopotential signals, such as electrooculography (EOG), electrocardiogram (ECG), and electroencephalogram (EEG). To prevent direct skin contact with carbon nanotubes, we apply various packaging materials, including polydimethylsiloxane (PDMS), Eco-flex, polyimide (PI), and polyurethane (PU). We conduct a comparative analysis of their signal-to-noise ratios in comparison to conventional gel electrodes. Our system demonstrates real-time biopotential monitoring for continuous health tracking, utilizing CPC in conjunction with a portable data acquisition system. The collected data are analyzed to provide accurate heart rates, respiratory rates, and heart rate variability metrics. Additionally, we explore the feasibility using CPC for sleep monitoring by collecting EEG signals.

Rapid detection of indoor airborne viruses is critical to prevent the spread of respiratory diseases. Herein, we present sensitive, highly rapid electrochemical measurement of airborne coronaviruses through condensation-based direct impaction onto antibody-immobilized, carbon nanotube-coated porous paper working electrodes (PWEs). Carboxylated carbon nanotubes are drop-cast on paper fibers to make three-dimensional (3D) porous PWEs. These PWEs have higher active surface area-to-volume ratios and electron transfer characteristics than conventional screen-printed electrodes. The limit of detection and detection time of the PWEs for liquid-borne coronaviruses OC43 are 65.7 plaque-forming units (PFU)/mL and 2 min, respectively. The PWEs showed sensitive and rapid detection of whole coronaviruses, which can be ascribed to the 3D porous electrode structure of the PWEs. Moreover, water molecules condense on airborne virus particles during air sampling, and these water-encapsulated virus particles (<4 µm) are impacted on the PWE for direct measurement without virus lysis and elution. The whole detection takes ∼10 min, including air sampling, at virus concentrations of 1.8 and 11.5 PFU/L of air, which can be due to the highly enriching and minimally damaging virus capture on a soft and porous PWE, demonstrating the potential for the rapid and low-cost airborne virus monitoring system., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Many studies have been conducted on measuring avian influenza viruses and their hemagglutinin (HA) antigens via electrochemical principles; most of these studies have used gold electrodes on ceramic, glass, or silicon substrates, and/or labeling for signal enhancement. Herein, we present a paper-based immunosensor for label-free measurement of multiple avian influenza virus (H5N1, H7N9, and H9N2) antigens using flexible screen-printed carbon nanotube-polydimethylsiloxane electrodes. These flexible electrodes on a paper substrate can complement the physical weakness of the paper-based sensors when wetted, without affecting flexibility. The relative standard deviation of the peak currents was 1.88% when the electrodes were repeatedly bent and unfolded twenty times with deionized water provided each cycle, showing the stability of the electrodes. For the detection of HA antigens, approximately 10-μl samples (concentration: 100 pg/ml-100 ng/ml) were needed to form the antigen-antibody complexes during 20-30 min incubation, and the immune responses were measured via differential pulse voltammetry. The limits of detections were 55.7 pg/ml (0.95 pM) for H5N1 HA, 99.6 pg/ml (1.69 pM) for H7N9 HA, and 54.0 pg/ml (0.72 pM) for H9N2 HA antigens in phosphate buffered saline, and the sensors showed good selectivity and reproducibility. Such paper-based sensors are economical, flexible, robust, and easy-to-manufacture, with the ability to detect several avian influenza viruses., (© 2022. The Author(s).)

Herein, we report results of the studies relating to the development of an impedimetric, magnetic bead-assisted supersandwich DNA hybridization assay for ultrasensitive detection of Neisseria gonorrhoeae , the causative agent of a sexually transmitted infection (STI), gonorrhea. First, a conductive ink was formulated by homogenously dispersing carboxylated multiwalled carbon nanotubes (cMWCNTs) in a stable emulsion of terpineol and an aqueous suspension of carboxymethyl cellulose (CMC). The ink, labeled C5 , was coated onto paper substrates to fabricate C5 @paper conductive electrodes. Thereafter, a magnetic bead (MB)-assisted supersandwich DNA hybridization assay was optimized against the porA pseudogene of N. gonorrhoeae . For this purpose, a pair of specific 5' aminated capture probes (SCP) and supersandwich detector probes (SDP) was designed, which allowed the enrichment of target gonorrheal DNA sequence from a milieu of substances. The SD probe was designed such that instead of 1:1 binding, it allowed the binding of more than one T strand, leading to a 'ladder-like' DNA supersandwich structure. The MB-assisted supersandwich assay was integrated into the C5 @paper electrodes for electrochemical analysis. The C5 @paper electrodes were found to be highly conductive by a four-probe conductivity method (maximum conductivity of 10.1 S·cm -1 ). Further, the biosensing assay displayed a wide linear range of 100 aM-100 nM (10 9 orders of magnitude) with an excellent sensitivity of 22.6 kΩ·(log[concentration]) -1 . The clinical applicability of the biosensing assay was assessed by detecting genomic DNA extracted from N. gonorrhoeae in the presence of DNA from different non-gonorrheal bacterial species. In conclusion, this study demonstrates a highly sensitive, cost-effective, and label-free paper-based device for STI diagnostics. The ink formulation prepared for the study was found to be highly thixotropic, which indicates that the paper electrodes can be screen-printed in a reproducible and scalable manner.

A paper-based chemiresistive sensor consisting of a single-walled carbon nanotubes network with electrodeposited gold nanoparticles (AuNPs-SWCNTs) was developed for the sensitive detection of As(III). A new sensing strategy was also proposed combined with a paper-based device for As(III) chemiresistive detection based on the electrochemical doping of As(0) on the AuNPs-SWCNTs channel. The proposed paper-based chemiresistive sensor was manufactured by wax printing and vacuum deposition using a water-based SWCNTs ink. The sensor sensitivity was enhanced by optimizing the volume of SWCNTs ink used in the formation of the sensing network in the paper and the sweep segment of cyclic voltammetry used for the electrochemical deposition of AuNPs. Additionally, the mechanism of the sensing strategy based on the electrochemical n-doping of As(0) to the AuNPs-SWCNTs sensing channel was investigated using a liquid-ion gated FET system. The As(III) concentration detected by the as-fabricated chemiresistive sensor is as low as 1.35 × 10 -5  μM which is even lower than the maximum permissible concentration in drinking water defined by the WHO. In this paper, we provide a simple strategy for developing paper-based chemiresistive sensors with unique properties for practical As(III) determination., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

We propose a unique soft actuator-a paper actuator-based on carbon-nanotube-composite paper (CNT-composite paper), which is a composite of carbon nanotubes (CNTs) and paper. CNT-composite paper has highly efficient properties because of the contained CNTs, such as high electrical conductivity and semiconducting properties. We are considering using CNT-composite paper for various devices. In this study, we successfully developed a paper actuator. We determined the structure of the paper actuator by referencing that of bucky-gel actuators. The actuator operates using the force generated by the movement of ions. In addition to making the paper actuator, we also attempted to improve its performance, using pressure as an index and an electronic scale to measure the pressure. We investigated the optimal dispersant for use in paper actuators, expecting the residual dispersant on the CNT-composite paper to affect the performance differently depending on the type of dispersant. Referring to research on bucky-gel actuators, we also found that the addition of carbon powder to the electrode layers is effective in improving the pressure for paper actuators. We believe that the paper actuator could be used in various situations due to its ease of processing.

In order to develop environmentally friendly, economical and facile preparation method of carbon aerogels (CAs), the waste printing paper as the raw material was combined with graphene oxide and carboxylic multi-walled carbon nanotubes to produce CAs (ρ = 44 mg cm -3 ). The CAs with different composition were investigated, the addition of graphene oxide led to the reduction of adsorption sites and the reduction of extraction performance. But the carbon nanotubes made CAs have a better pore structure. The CAs as adsorbent were loaded into a pipette-tip for solid-phase extraction of hexaconazole and diniconazole. Coupled with gas chromatography, an analytical method was established under the optimized conditions. The limits of detection were between 0.08 and 0.32 mg kg -1 , the linear ranges were 0.96-200.0 mg kg -1 and 0.24-200.0 mg kg -1 . The relative recoveries were in the range of 81.0-119%. The results indicated that the method had potential application for the determination of triazole fungicides., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Herein, a paper-based glucose/air biofuel cell (BFC) was constructed and implemented for self-powered pesticide detection. Our developed paper-based chip relies on a hollow-channel to transport fluids rather than capillarity, which reduces analysis times as well as physical absorption. The gold nanoparticles (Au NPs) and carbon nanotubes (CNTs) were adapted to modify the paper fibers to fabricate the flexible conductive paper anode/cathode electrode (Au-PAE/CNT-PCE). Molecularly imprinted polymers (MIPs) using 2,4-dichlorophenoxyacetic acid (2,4-D) as a template were synthesized on Au-PAE for signal control. In the cathode, bilirubin oxidase (BOD) was used for the oxygen reduction reaction. Based on a competitive reaction between 2,4-D and glucose-oxidase-labeled 2,4-D (GOx-2,4-D), the amount of GOx immobilized on the bioanode can be simply tailored, thus a signal-off self-powered sensing platform was achieved for 2,4-D determination. Meanwhile, the coupling of the paper supercapacitor (PS) with the paper-based chip provides a simple route for signal amplification. Combined with a portable digital multi-meter detector, the amplified signal can be sensitively readout. Through rational design of the paper analytical device, the combination of BFC and PS provides a new prototype for constructing a low-cost, simple, portable, and sensitive self-powered biosensor lab-on-paper, which could be easily expanded in the field of clinical analysis and drug delivery.

Flexible paper-based sensors may be applied in numerous fields, but this requires addressing their limitations related to poor thermal and water resistance, which results in low service life. Herein, we report a paper-based composite sensor composed of carboxylic carbon nanotubes (CCNTs) and poly- m -phenyleneisophthalamide (PMIA), fabricated by a facile papermaking process. The CCNT/PMIA composite sensor exhibits an ability to detect pressures generated by various human movements, attributed to the sensor's conductive network and the characteristic "mud-brick" microstructure. The sensor exhibits the capability to monitor human motions, such as bending of finger joints and elbow joints, speaking, blinking, and smiling, as well as temperature variations in the range of 30-90 °C. Such a capability to sensitively detect pressure can be realized at different applied frequencies, gradient sagittas, and multiple twists with a short response time (104 ms) even after being soaked in water, acid, and alkali solutions. Moreover, the sensor demonstrates excellent mechanical properties and hence can be folded up to 6000 times without failure, can bear 5 kg of load without breaking, and can be cycled 2000 times without energy loss, providing a great possibility for a long sensing life. Additionally, the composite sensor shows exceptional Joule heating performance, which can reach 242 °C in less than 15 s even when powered by a low input voltage (25 V). From the perspective of industrialization, low-cost and large-scale roll-to-roll production of the paper-based sensor can be achieved, with a formed length of thousands of meters, showing great potential for future industrial applications as a wearable smart sensor for detecting pressure and temperature, with the capability of electric heating.

Hypocalcemia is caused by a sharp decline in blood calcium concentration after dairy cow calving, which can lead to various diseases or even death. It is necessary to develop an inexpensive, easy-to-operate, reliable sensor to diagnose hypocalcemia. The cellulose-paper-based microfluidic field-effect biosensor is promising for point-of-care, but it has poor mechanical strength and a short service life after exposure to an aqueous solution. Octadecyltrichlorosilane (OTS), as a popular organosilane derivative, can improve the hydrophobicity of cellulose paper to overcome the shortage of cellulose paper. In this work, OTS was used to produce the superhydrophobic cellulose paper that enhances the mechanical strength and short service life of MFB, and a microfluidic field-effect biosensor (MFB) with semiconducting single-walled carbon nanotubes (SWNTs) and DNAzyme was then developed for the Ca 2+ determination. Pyrene carboxylic acid (PCA) attached to SWNTs through a non-covalent π-π stacking interaction provided a carboxyl group that can bond with an amino group of DNAzyme. Two DNAzymes with different sensitivities were designed by changing the sequence length and cleavage site, which were functionalized with SPFET/SWNTs-PCA to form Dual-MFB, decreasing the interference of impurities in cow blood. After optimizing the detecting parameters, Dual-MFB could determine the Ca 2+ concentration in the range of 25 μM to 5 mM, with a detection limit of 10.7 μM. The proposed Dual-MFB was applied to measure Ca 2+ concentration in cow blood, which provided a new method to diagnose hypocalcemia after dairy cow calving.

Owing to its critical role in the development of female reproductive tissues and as a clinical biomarker, there is an urgent need to develop a rapid and cost-effective method to sensitively detect 17β-estradiol (E2). In this work, a folding aptasensor platform with microfluidic channels for the label-free electrochemical detection of E2 is described. The platform, designed with a delicate folding structure, integrating filter holes, microfluidic channels, reaction chambers, and a three-electrode system, is extremely easy to use. To increase the detection sensitivity and immobilize the aptamer, we synthesized a novel nanoassembly consisting of amine-functionalized single-walled carbon nanotube/new methylene blue/gold nanoparticles (AuNPs) and modified the working electrode with this nanoassembly. The calibration curve obtained from the experimental results exhibited a linear range between 10 pg mL -1 and 500 ng mL -1 ( R 2 was achieved (S/N = 3). Furthermore, experiments to detect E2 in clinical serum were conducted, and the results were highly similar to those obtained using a large electrochemical luminescence apparatus. By integrating multiple functional components, adopting novel nanoassemblies, and using a folding structure, this paper-based platform not only has great potential as a simple and convenient integrated device for point-of-care testing of E2, but also as a portable, low-cost, and highly sensitive aptasensor platform capable of detecting many diagnostic biomarkers with the appropriate aptamers.-1 was achieved (S/N = 3). Furthermore, experiments to detect E2 in clinical serum were conducted, and the results were highly similar to those obtained using a large electrochemical luminescence apparatus. By integrating multiple functional components, adopting novel nanoassemblies, and using a folding structure, this paper-based platform not only has great potential as a simple and convenient integrated device for point-of-care testing of E2, but also as a portable, low-cost, and highly sensitive aptasensor platform capable of detecting many diagnostic biomarkers with the appropriate aptamers.

Hybrid nanomaterials fabricated by the heterogeneous integration of 1D (carbon nanotubes) and 2D (graphene oxide) nanomaterials showed synergy in electrical and mechanical properties. Here, we reported the infiltration of carboxylic functionalized single-walled carbon nanotubes (C-SWNT) into free-standing graphene oxide (GO) paper for better electrical and mechanical properties than native GO. The stacking arrangement of GO sheets and its alteration in the presence of C-SWNT were comprehensively explored through scanning electron microscopy, X-ray photoelectron spectroscopy (XPS) and X-ray diffraction. The C-SWNTs bridges between different GO sheets produce a pathway for the flow of electrical charges and provide a tougher hybrid system. The nanoscopic surface potential map reveals a higher work function of the individual functionalised SWNTs than surrounded GO sheets showing efficient charge exchange. We observed the enhanced conductivity up to 50 times and capacitance up to 3.5 times of the hybrid structure than the GO-paper. The laminate of polystyrene composites provided higher elastic modulus and mechanical strength when hybrid paper is used, thus paving the way for the exploitation of hybrid filler formulation in designing polymer composites.

Developing a cost-effective and simple micro-analysis tool has long been an important objective in the toxicological detection of fluorene. In this study, a disposable paper-based micro-analysis device (μ-PAD) was designed using graphite doped with multi-walled carbon nanotubes (MWCNTs) to hand draw the electrode (MWCNTs/μ-PAD). We investigated the feasibility of the designed MWCNTs/μ-PAD as a cell-sensing platform using voltammetry measurements. Its application for evaluating the cytotoxicity of fluorene was studied based on the electrochemical determination of human breast cancer cells induced by fluorene. The results showed a dose-dependent toxicity effect of fluorene on cellular activity. The measurements were comparable with those obtained using a methyl-thiazolyl-tetrazolium assay. The detection limit of the MWCNTs/μ-PAD for human breast cancer cells was as low as 4.00 × 10 3 cells·mL -1 owing to the enhanced catalytic activity of the MWCNTs. Notably, the MWCNTs/μ-PAD-which had a diameter of 7.00 mm-allowed a sampling volume of 10.0 μL, which is 50.0 times less than the sampling volume required with a conventional electrode (500 μL). Advantages such as the simplicity of manufacture, low consumption, low cost, rapid detection, and disposability, suggest that the MWCNTs/μ-PAD could provide new opportunities and directions for in vitro microanalysis., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)

To transform tall goldenrods, which are invasive alien plant that destroy the ecosystem of South Korea, into useful materials, cellulose fibers isolated from tall goldenrods are applied as EMI shielding materials in this study. The obtained cellulose fibers were blended with CNTs, which were used as additives, to improve the electrical conductivity. TGCF/CNT papers prepared using a facile paper manufacturing process with various weight percent ratios and thickness were carbonized at high temperatures and investigated as EMI shielding materials. The increase in the carbonization temperature, thickness, and CNT content enhanced the electrical conductivity and EMI SE of TGCF/CNT carbon papers. TGCF/CNT-15 papers, with approximately 4.5 mm of thickness, carbonized at 1300 °C exhibited the highest electrical conductivity of 6.35 S cm -1 , indicating an EMI SE of approximately 62 dB at 1.6 GHz of the low frequency band. Additionally, the obtained TGCF/CNT carbon papers were flexible and could be bent and wound without breaking.

Herein, a so-called carbon nanotube (CNT) electrode was printed in on a paper substrate using the handwriting technique and carbon nanotube ink in a marker pen to print the working electrode, graphite pencil to print the counter electrode and graphite/silver nanoparticle (AgNP) ink in a rollerball pen to print the quasi-reference electrode. The carbon nanotube electrode was characterized via scanning electron microscopy. The electrode was optimized based on the type of paper, hydrophobic barrier and number of layers. In summary, the optimized parameters included the use of matte paper with a mineral spirit layer. The number of carbon nanotube layers to achieve the best electrochemical performance was 25. The final graphite electrode was a miniaturized and flexible paper-based electrochemical electrode. To evaluate the electrical properties of the electrodes, the ohmic resistance of each ink was tested using a multimeter and the obtained values were 18.62 kΩ for the CNT ink, 1.53 Ω for the AgNP ink and 3.53 kΩ for the graphite trace. These results indicate the good conductivity of each synthesized ink used in the fabrication of the CNT electrode. Finally, the electrode was used to measure the electrochemical response of different concentrations of K 4 [Fe(CN) 6 ]. Then, a calibration curve was obtained from the voltammograms and linearity was observed in the range of 0.5-3.5 mM. This suggests that the CNT electrode has the potential to be used as an amperometric electrode.

In this work, a flexible amphiprotic amino-bonded carbon nanotube-Ag nanoparticle/polystyrene (CNT-NH 2 -Ag/PS) paper electrode was fabricated to measure glucose in human body fluids by a combination of vacuum filtration and high temperature baking. The front side of the fabricated paper electrode was hydrophobic and conductive, whereas its back side was hydrophilic and nonconductive. In the fabrication process, the coating sequence of CNT-NH 2 , Ag and PS was critical to determine the performance of the resulting CNT-NH 2 -Ag/PS electrode besides other parameters ( e.g. , amount of soluble starch, PS and Ag nanoparticles, type and amount of CNT-NH 2 , and electrode sensing area). Based on a series of experimental observations, the possible mechanism of glucose detection on the paper electrode was proposed, in which glucose was more favorable to migrate to the hydrophilic back side of the paper and interact with the active species ( e.g. , O 2 - -Ag/PS paper electrode maintained stable electrochemical properties even after five cycles of use and 60 days of storage in air. The amphiprotic paper electrode demonstrated excellent sensing performance for glucose with a linear range of 1 μM to 1000 μM, a low detection limit of 0.2 μM, and a sensitivity of 31 333.0 μA mM 2 -Ag/PS paper electrode maintained stable electrochemical properties even after five cycles of use and 60 days of storage in air. The amphiprotic paper electrode demonstrated excellent sensing performance for glucose with a linear range of 1 μM to 1000 μM, a low detection limit of 0.2 μM, and a sensitivity of 31 333.0 μA mM -1 . The fabricated paper electrode was also successfully applied to detect different levels of glucose in complex human body fluids such as saliva, urine, and serum. These features make this type of paper electrode promising for glucose measurement.-2 . The fabricated paper electrode was also successfully applied to detect different levels of glucose in complex human body fluids such as saliva, urine, and serum. These features make this type of paper electrode promising for glucose measurement.

Both programmed death-1 (PD-1) and programmed death-ligand 1 (PD-L1) are important proteins in cancer immunotherapy. Soluble forms (sPD-1 and sPD-L1) have potential for determining treatment and prognosis monitoring. However, there is a lack of detection methods for point-of-care testing (POCT) of these two proteins, so a low-cost rapid detection platform is urgently needed. To solve this problem, a dual-channel electrochemical platform, including a folding paper-based immunosensor and a POCT system for rapid simultaneous detection of these two proteins was designed and fabricated. The immunosensor consists of a three-electrode system and a reaction cell. The surface of the working electrode was modified with nanocomposites synthesized from amine-functionalized single-walled carbon nanotubes, new methylene blue, and gold nanoparticles. Antibodies to sPD-1 and sPD-L1 were also immobilized on the working electrode surface. A differential pulse voltammetry electrochemical method was adopted. The immunosensor was able to detect sPD-1 and sPD-L1 in the ranges of 50 pg/mL to 50 ng/mL and 5 pg/mL to 5 ng/mL, respectively. The limits of detection were 10 and 5 pg/mL. Using this detection platform, sPD-1 and sPD-L1 in plasma were detected by both enzyme-linked immunosorbent assay and the immunosensor, which has good application potential.

Rapid and accurate clinical assessment of hemostasis is essential for managing patients who undergo invasive procedures, experience hemorrhages, or receive antithrombotic therapies. Hemostasis encompasses an ensemble of interactions between the cellular and non-cellular blood components, but current devices assess only partial aspects of this complex process. In this work, we describe the development of a new approach to simultaneously evaluate coagulation function, platelet count or function, and hematocrit using a carbon nanotube-paper composite (CPC) capacitance sensor. CPC capacitance response to blood clotting at 1.3 MHz provided three sensing parameters with distinctive sensitivities towards multiple clotting elements. Whole blood-based hemostasis assessments were conducted to demonstrate the potential utility of the developed sensor for various hemostatic conditions, including pathological conditions, such as hemophilia and thrombocytopenia. Results showed good agreements when compared to a conventional thromboelastography. Overall, the presented CPC capacitance sensor is a promising new biomedical device for convenient non-contact whole-blood based comprehensive hemostasis evaluation., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Flexible electronics have gained more attention for emerging electronic devices such as sensors, biosensors, and batteries with advantageous properties including being thin, lightweight, flexible, and low-cost. The development of various forms of allotropic carbon papers provided a new dry-manufacturing route for the fabrication of flexible and wearable electronics, while the electrochemical performance and the bending stability are largely influenced by the bulk morphology and the micro-/nanostructured domains of the carbon papers. Here, we evaluate systematically the intrinsic physicoelectrochemical properties of allotropic carbon-based conducting papers as flexible electrodes including carbon-nanotubes-paper (CNTs-paper), graphene-paper (GR-paper), and carbon-fiber-paper (CF-paper), followed by functionalization of the allotropic carbon papers for the fabrication of flexible electrodes. The morphology, chemical structure, and defects originating from the allotropic nanostructured carbon materials were characterized by scanning electron microscopy (SEM) and Raman spectroscopy, followed by evaluating the electrochemical performance of the corresponding flexible electrodes by cyclic voltammetry and electrochemical impedance spectroscopy. The electron-transfer rate constants of the CNTs-paper and GR-paper electrodes were ∼14 times higher compared with the CF-paper electrode. The CNTs-paper and GR-paper electrodes composed of nanostructured carbon showed significantly higher bending stabilities of 5.61 and 4.96 times compared with the CF-paper. The carbon-paper flexible electrodes were further functionalized with an inorganic catalyst, Prussian blue (PB), forming the PB-carbon-paper catalytic electrode and an organic conducting polymer, poly(3,4-ethylenedioxythiophene) (PEDOT), forming the PEDOT-carbon-paper capacitive electrode. The intrinsic attribute of different allotropic carbon electrodes affects the deposition of PB and PEDOT, leading to different electrocatalytic and capacitive performances. These findings are insightful for the future development and fabrication of advanced flexible electronics with allotropic carbon papers.

We propose a flexible, binder-free and free-standing carbonaceous paper fabricated via electrostatic spray deposition using reduced graphene oxide/carbon nanotube (rGO/CNT) as a promising electrode material for flexible sodium-ion batteries (NIBs). The as-prepared rGO/CNT paper exhibits a three-dimensional (3D) layered structure by employing rGO as conductive frameworks to provide sodium-storage active sites and CNT as spacer to increase rGO interlayer distance and benefit the diffusion kinetics of sodium ions. Consequently, the rGO/CNT paper delivers an enhanced sodium ion storage capacity of 166.8 mAh g -1 at 50 mA g -1 , retaining an average capacity of 101.4 mAh g -1 when current density sets back 100 mA g -1 after cycling at various current rates. An average capacity of 50 mAh g -1 at 200 mA g -1 was stabilized when cycling up to 300 cycles. The well-maintained electrochemical performance of free-standing rGO/CNT paper is due to the well-established hybrid 3D nanostructures, which demonstrates our carbon based material fabricated by a facile approach can be applied as one of the high-performance and low-cost electrode materials for applications in flexible energy storage devices., Competing Interests: The authors declare no conflicts of interest.

Microcystin-LR (MC-LR) is a cyanobacterial toxin produced as a result of eutrophication in polluted water in warm weather conditions. The MC-LR could cause health problems in mammal organs such as the liver, heart, and muscle. Therefore, the World Health Organization (WHO) has stipulated a limit of <1.0 ng/mL in drinking water. Thus, detection and quantification are vital, but current techniques require complex and expensive offsite analysis. We have developed an inexpensive, sensitive, and field-deployable sensor based on bioactivated multiwalled carbon nanotubes (MWCNTs, diameter 20 nm) and micropore filter paper (0.45-μm pore size) for the detection of MC-LR. A specially designed DNA oligonucleotide (5-NH 2 -C 6 -AN 6 ) was used as the MC-LR targeting aptamer (MCTA). For bioactivation, MCTA was immobilized on the carboxylated MWCNTs via the formation of amide bonds. The bioactivated MWCNTs were deposited on the micropore filter paper by suction filtering. The detection of MC-LR in freshwater was possible within 1.5 h, achieved by measuring the changes in electrical resistance caused by the selective MC-LR and MCTA interactions. Despite suffering from some matrix effects, the detection limit of the sensor was 0.19 ng/mL for low-concentration MC-LR (≤0.5 ng/mL). This method is much cheaper (biosensor price: < $2.5) than liquid chromatography coupled with tandem mass spectroscopy analysis (ca. $50/sample) which is a standard method for MC-LR detection in a modern laboratory. Thus, this cheap and straightforward MC-LR sensor has applications for detection in remote locations., (Copyright © 2021 Elsevier B.V. All rights reserved.)

The development of an affordable chemiresistive biosensor enhanced with a multi-walled carbon nanotube-zinc oxide (MWCNT-ZnO) nanofiber composite is presented. The sensor leverages the precise interaction between lipoarabinomannan (LAM) tuberculosis (TB) antigens and antibodies to achieve high sensitivity and specificity. The MWCNT-ZnO nanofibers have a larger surface area and better electrical conductivity, which makes it easier for TB antibodies to stick to them. The binding of LAM TB antigens to the fixed Monoclonal Antibody-MBS320597 induces significant resistance changes in the chemiresistive sensor, enabling accurate TB detection. Performance evaluation reveals a linear detection range from 1.0 to 100.0 pg/mL in the lower concentration range and up to 6.0 ng/mL in the higher concentration range, with a sensitivity of 79.750 mA pg mL -1  cm -2 and a lower limit of detection of 40.54 fg/mL. The sensor exhibits a response time of 102 s. Featuring rapid response time and high sensitivity, this biosensor is ideally suited for point-of-care (PoC) applications. The incorporation of MWCNT-ZnO nanofibers shows great potential for enhancing the development of sensitive and cost-effective TB diagnostic tools, which could play a crucial role in advancing global TB control and management efforts., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)

It is very important to develop a rapid, simple, low cost point-of-care (POC) method for the early diagnosis of pathogens. In this work, a flexible paper-based electrode based on nickel metal-organic framework (Ni-MOF) composite/Au nanoparticles/carbon nanotubes/polyvinyl alcohol (Ni-Au composite/CNT/PVA) was constructed to detect target human immunodeficiency virus (HIV) DNA by DNA hybridization using methylene blue (MB) as a redox indicator. The CNT/PVA and Ni-Au composite were deposited on the cellulose membrane by vacuum filtration and drop-coating method in turn to obtain Ni-Au composite/CNT/PVA (CCP) film electrode. Compared to the CNT/PVA film electrode, CCP film electrode makes a higher loading of the probe DNA for its large specific surface area and conjugated π-electron system that can provide hydrogen bond sources to achieve interactions between MOF and single-stranded DNA, which improves the sensitivity for detecting target DNA. The variation of peak current for MB molecules adsorbed onto DNA before and after hybridization with HIV DNA was monitored. Electrochemical results proved that the CCP film maintained stable electrochemical property even after bending 200 times or stretching under different strains from 0% to 20%. The flexible paper electrode showed excellent sensing performance with a linear range of 10 nM-1 μM and a low detection limit of 0.13 nM. The target HIV DNA was successfully detected even in complex serum samples using the flexible CCP film electrode. Therefore, the simple and inexpensive flexible paper-based MOF composite film electrode can also be utilized for other pathogens POC diagnosis., (Copyright © 2021. Published by Elsevier B.V.)

This paper establishes a membraneless, co-laminar flow-based approach to develop a cost-effective microfluidic paper-based analytical device for enzymatic biofuel cell ( PAD-EBFC). The developed PAD-EBFC supporting the self-capillary fluid transport action consists of Y-shaped paper microchannel with the fuel (glucose) and oxidant (O 2 ) streaming in parallel over carbon nanotube-based bucky paper electrodes modified with biocompatible electrocatalytic enzymes, such as glucose oxidase and laccase without any additional redox cofactor. The electrochemical performance for the modified bioelectrodes, i.e., electrocatalytic oxidation and reduction reaction, was carried out using linear sweep voltammetry, cyclic voltammetry, and open circuit potential. The overall performance of PAD-EBFC was evaluated using the polarization studies. Subsequently, the catalytic activity of enzymes on the electrode surface was validated by the scanning electron microscope. This simple and portable PAD-EBFC can generate the maximum power density to the order of /cm 2 ( /cm 2 ) at 0.505 V over prolonged durations of around 50 h. Hence, the presented PAD-EBFC shows good power density and stability, leading to its strong potential to power miniaturized microelectronics devices and sensors.

This paper demonstrates a simple-in-struct- ure, cost-effective, and environment-friendly Microfluidic Paper-based Analytical Device for Microbial Biofuel Cell ( μ PAD-MBFC). It consists of a microchannel with biofuel cell (Escherichia.Coli) and an oxidant (aerated tap water) flowing co-parallelly over Multiwalled Carbon Nanotube (MWCNT)-based Bucky Paper (BP) electrodes using a self-capillary and co-laminar flow mechanism. The electrochemical studies, such as open circuit potential (OCP) and polarization were evaluated using a potentiostat. Various volumetric bacterial studies were also carried out to find out the best suitable optimal bacterial volume. Subsequently, the morphological and detailed element composition study of electrode surface was performed using scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) techniques. This well-designed portable μ PAD-MBFC yields a maximum power density of 4 [Formula: see text]/cm 2 ( [Formula: see text]/cm 2 ) at 0.405 V over [Formula: see text] of culture and leveraging to its long-lasting potential to operate miniaturized microelectronics sensors and portable devices.

Electronic noses (e-nose) and optical noses (o-nose) are two emerging approaches for the development of artificial olfactory systems for flavor and smell evaluation. The current work leverages the unique optical properties of semiconducting single-wall carbon nanotubes (SWCNTs) to develop a prototype of a novel paper-based near-infrared optical nose (NIRON). We have drop-dried an array of SWCNTs encapsulated with a wide variety of peptides on a paper substrate and continuously imaged the emitted SWCNTs fluorescence using a CMOS camera. Odors and different volatile molecules were passed above the array in a flow chamber, resulting in unique modulation patterns of the SWCNT photoluminescence (PL). Quartz crystal microbalance (QCM) measurements performed in parallel confirmed the direct binding between the vapor molecules and the peptide-SWCNTs. PL levels measured before and during exposure demonstrate distinct responses to the four tested alcoholic vapors (ethanol, methanol, propanol, and isopropanol). In addition, machine learning tools directly applied to the fluorescence images allow us to distinguish between the aromas of red wine, beer, and vodka. Further, we show that the developed sensor can detect limonene, undecanal, and geraniol vapors, and differentiate between their smells utilizing the PL response pattern. This novel paper-based optical biosensor provides data in real-time, and is recoverable and suitable for working at room temperature and in a wide range of humidity levels. This platform opens new avenues for real-time sensing of volatile chemical compounds, odors, and flavors., (Copyright © 2020 Elsevier B.V. All rights reserved.)

The performance of microbial electrochemical system for hexavalent chromium (Cr(VI)) contaminant has been a severe challenge remaining active for further development. In this study, we developed a novel biochar material from industrial paper sludge for microbial fuel cell cathode fabrication to reduce aquatic Cr(VI) to non-toxic Cr(III). With additive melamine as nitrogen source and self-containing small portion of Fe as catalyst, the sludge evolved into electroactive biochar (BC-M) rendering a unique N-doped carbon nanotubes/activated carbon (N-CNT/AC) frame after pyrolyzed at 900 °C for 2 h. Electrochemical analysis revealed enhanced electron transference capacity of this composite material, such effectiveness was attributed to the increased surface area and superior electroconductivity of N-doped CNTs. For performance of Cr(VI) reduction, a 55.1% reduction efficiency was achieved in an microbial fuel cell equipped with BC-M cathode while it reduced to about 41.8% when the cathode was replaced by electrode modified with no-melamine-involved biochar. The strategy of biochar upgrading from industrial paper sludge proposed in this work is expected to not only bring technical solution for low-cost CNT materials preparation for Cr(VI) reduction, but also put forward further research on value-added chemical synthesis from waste in various fields of energy and environment., Competing Interests: Declaration of competing interest There are no conflicts of interests to declare., (Copyright © 2020 Elsevier B.V. All rights reserved.)

This paper introduces a cheap simple MWCNTs@paper biosensor for the detection of CA19-9, which is a biomarker of pancreatic cancer. By adding the CA19-9 antibody to the surface of MWCNTs which are deposited on the microporous filter paper, the correlation between the concentration of CA19-9 and resistance of biosensor element was linear due to the site-specific binding of antigen and antibody. The detection range is wide (0 U/mL-at least 1000 U/mL), and even in the low concentration of CA19-9, the linearity remains satisfying. Based on this property, it could be used for the detection of early-stage pancreatic cancer. Besides, this research originally introduces a vacuum freeze-drying method for the long-term preservation of biosensor, prolonging its storage time from 3 h to at least 7 days, which signifcantly promoted its value in practical application. One piece of the MWCNTs@paper biosensor only cost $2 (about 30 times cheaper than ELISA) approximately, and the detection speed is satisfying (2 h, 12 times faster than ELISA), which will possibly increase its opportunity of mass production and clinical practice., Competing Interests: Declaration of Competing Interest The authors declared that there is no conflict of interest., (Copyright © 2019 Elsevier B.V. All rights reserved.)

A protease is an enzyme that catalyzes proteolysis of proteins into smaller polypeptides or single amino acids. As crucial elements in many biological processes, proteases have been shown to be informative biomarkers for several pathological conditions in humans, animals, and plants. Therefore, fast, reliable, and cost-effective protease biosensors suitable for point-of-care (POC) sensing may aid in diagnostics, treatment, and drug discovery for various diseases. This work presents an affordable and simple paper-based dipstick biosensor that utilizes peptide-encapsulated single-wall carbon nanotubes (SWCNTs) for protease detection. Upon enzymatic digestion of the peptide, a significant drop in the photoluminescence (PL) of the SWCNTs was detected. As the emitted PL is in the near-infrared region, the developed biosensor has a good signal to noise ratio in biological fluids. One of the diseases associated with abnormal protease activity is pancreatitis. In acute pancreatitis, trypsin concentration could reach up to 84 µg/mL in the urine. For proof of concept, we demonstrate the feasibility of the proposed biosensor for the detection of the abnormal levels of trypsin activity in urine samples.

Spheres, tubes, and planar-shaped nanomaterials as Fe3O4 nanoparticles (MNPs), multiwalled carbon nanotubes (MWCNT), and graphene oxide (GO) were used for the first time to treat microfluidic paper-based analytical devices (μPADs) and create a biocompatible layer with high catalytic surface. Once glucose measurements are critical for diabetes or glycosuria detection and monitoring, the analytical performance of the proposed devices was studied by using bienzymatic colorimetric detection of this carbohydrate. The limit of detection values achieved for glucose with μPADs treated with MNPs, MWCNT, and GO were 43, 62, and 18 μM, respectively. The paper surface modification solves problems associated with the lack of homogeneity on color measurements that compromise the sensitivity and detectability levels in clinical diagnosis.

We reported a highly conductive nanocomposite made with multiwalled carbon nanotubes (MWCNTs) and chitin nanofibers (ChNFs). The MWCNTs were dispersed into ChNFs by the simple process of vacuum-filtration, forming a three-dimensional network structure. In this approach, MWCNT acted as a filler to introduce electron channel paths throughout the ChNF skeleton. And then, a hybrid hydrogel system (20 wt.% NaOH, -18 °C) was applied to prepare the ChNF/MWCNT gel-film followed with drying process. It is found that the resultant ChNF/MWCNT gel-film exposed much more MWCNT areas forming denser structure due to the shrinking of ChNFs after the gelation treatment. Compared with ChNF/MWCNT film, the one treated under hydrogel system (ChNF/MWCNT gel-film) exhibited almost twice higher conductivity (9.3S/cm for 50 wt.% MWCNTs in gel-film; whereas 4.7S/cm for 50 wt.% MWCNTs in film). Moreover, the facile and low-cost of this conductive paper may have great potential in development of foldable electronic devices., (Copyright © 2015 Elsevier Ltd. All rights reserved.)

Paper-based biosensors are promising for low-cost diagnostics. However, its widespread use has been hampered due to a lack of sensitive detection methods that can be easily implemented on paper substrates. On the other hand, single-walled carbon nanotubes (SWNTs) -based chemiresistive biosensors are gaining popularity as label-free, highly sensitive biosensors. However, traditional SWNT-based chemiresistors need to be more affordable for use in resource-limited settings. In this study, we report fabrication, optimization and analytical characterization of a chemiresistive biosensor on paper for label-free immunosensing. We synthesized a water-based ink using pyrene carboxylic acid (PCA) through non-covalent π-π stacking interaction between PCA and SWNTs. The PCA/SWNTs ink concentration can reach ~4 mg mL -1 and was stable at room temperature for one month. We introduced a combination of wax printing and vacuum filtration to fabricate the hydrophilic channels and the well-defined PCA/SWNTs ink deposition on paper in a facile manner requiring no additional masks or stencils. Specific antibodies were then functionalized on the PCA/SWNTs. Quantitative and selective detection of human serum albumin (HSA) is demonstrated with a limit of detection (LOD) of 1 pM. This low LOD is attributed to the porous structure of the paper surface, which can accommodate more SWNTs. Furthermore, the hydroxyl group-containing cellulose fibers help connect the SWNTs into an electrical network. The paper-based chemiresistive biosensor proposed here is easy to fabricate, and designed for rapid, sensitive and selective detection of HSA. This work provides a potential platform for automated, disposable paper-based biosensors with multiplexed detection capability and microfluidic controls., (Copyright © 2018 Elsevier B.V. All rights reserved.)

This paper describes the development of an original micro-solid phase extraction device and its evaluation for the isolation of F2-isoprostanes (F2-IsoPs) from cord and maternal plasma samples. The unit is very simple and consists in a rotating disc (1.8 cm diameter) of oxidized buckypaper (BP), enwrapped in a polypropylene mesh pouch. Even if the selected F2-IsoPs have logP and pKa values that make them suitable candidates for their sorption on BP, several parameters were optimized to maximize recoveries: time of adsorption and desorption; stirring speed; volume, pH and ionic strength of the sample; type, volume, and fractions of the elution solvent; oxidation grade of BP. Among all, the last one was crucial in affecting extraction yields because of the analyte interactions with polar functionalities, introduced by a preliminary oxidative acid treatment. The investigation established the optimal oxidation time and highlighted the pros and cons of the acid activation step. All extracts were analyzed by means of liquid chromatography-tandem mass spectrometry (LC-MS/MS). Validation was performed according to the main FDA guidelines for bioanalytical methods. Depending on the spike level and analyte, recoveries ranged between 30 and 120% with precision and accuracy values lower than 20%. Quantitative analysis was accomplished by matrix-matched calibration curves whose determination coefficients were higher than 0.95. Lower limit of quantitation (LLOQ) spanned the range 2.45-6.77 μg L -1 . The validated method was applied to the analysis of eight pairs of mother/child plasma samples, revealing the presence of 8-iso-15-keto-PGF 2α and 8-iso-PGE 2 at a concentration of about 10 μg L -1 in most cord plasma samples of preterm newborns., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Copper-doped carbon dots and aminated carbon nanotubes (Cu-CDs/NH 2 -CNTs) nanocomposites were synthesized by a one-step growth method, and the composites were characterized for their performance. An electrochemical sensor for sensitive detection of bisphenol A (BPA) was developed for using Cu-CDs/NH 2 -CNTs nanocomposites modified with glassy carbon electrodes (GCE). The sensor exhibited an excellent electrochemical response to BPA in 0.2 M PBS (pH 7.0) under optimally selected conditions. The linear range of the sensor for BPA detection was 0.5-160 μM, and the detection limit (S/N = 3) was 0.13 μM. Moreover, the sensor has good interference immunity, stability and reproducibility. In addition, the feasibility of the practical application of the sensor was demonstrated by the detection of BPA in bottled drinking water and Liu Yang River water., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)

Alumina inorganic molecularly imprinted polymer (MIP) modified multi-walled carbon nanotubes (MWCNTs) on a glassy carbon electrode (MWCNTs-Al 2 O 3 -MIP/GCE) was firstly designed and fabricated by one-step electro deposition technique for the detection of uric acid (UA) in sweat. The UA templates were embedded within the inorganic MIP by co-deposition with Al 2 O 3 . Through the evaluation of morphology and structure by Field Emission Scanning Electron Microscope (SEM), Energy Dispersive X-ray Spectroscopy (EDS), X-ray Photoelectron Spectroscopy (XPS) and Transmission Electron Microscopy (TEM), it was verified that the specific recognition sites can be fabricated in the electrodeposited Al 2 O 3 molecular imprinted layer. Due to the high selectivity of molecular imprinting holes, the MWCNTs-Al 2 O 3 -MIP/GCE electrode demonstrated an impressive imprinting factor of approximately 2.338 compared to the non-molecularly imprinted glassy carbon electrode (MWCNTs-Al 2 O 3 -NIP/GCE) toward uric acid detection. Moreover, it exhibited a remarkable limit of detection (LOD) of 50 nM for UA with wide detection range from 50 nM to 600 μM. The MWCNTs-Al 2 O 3 -MIP/GCE electrode also showed strong interference resistance against common substances found in sweat. These results highlight the excellent interference resistance and selectivity of MWCNTs-Al 2 O 3 -MIP/GCE sensor, positioning it as a novel sensing platform for non-invasive uric acid detection in human sweat., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)

The in situ and direct extraction, desorption and ionization of in-gel intact proteins after electrophoresis has been achieved by carbon nanotubes (CNTs)-modified paper spray mass spectrometry at ambient conditions. Characteristics of CNTs (including larger surface area, smaller pore diameter and enhanced conductivity) were endowed to the porous filter paper substrate by uniformly dispersing the CNTs on the filter paper. Upon applying electric potential to the CNTs-modified paper, the in-gel proteins were extracted from the gel and subsequently migrated to the tip of the filter paper by electrophoresis-like behavior for paper spray ionization, which was monitored by extracted ion chronograms. The characterizations of modified filter papers and CNTs nanoparticles further confirmed the role of CNTs in in-gel protein extraction, protein migration as well as spray ionization at the paper tip. Under optimized conditions, a mixture of cytochrome c, lysozyme and myoglobin was successfully separated by native electrophoresis and subsequently analysed by the present method, showing a limit of detection of 10 ng per gel band. The present strategy offers a new pathway for the direct detection of in-gel intact proteins at ambient conditions without any pre-treatment (e.g. digestion, chemical extraction and desalting), which exhibits potential applications in top-down proteomics.

Paper-integrated configuration with miniaturized functionality represents one of the future main green electronics. In this study, a paper-based respiration sensor was prepared using a multiwalled carbon nanotube-templated nickel porphyrin covalent organic framework (MWCNTs@COF NiP-Ph ) as an electrical identification component and pencil-drawn graphite electric circuits as interdigitated electrodes (IDEs). The MWCNTs@COF NiP-Ph not only inherited the high gas sensing performance of porphyrin and the aperture induction effect of COFs but also overcame the shielding effect between phases through the MWCNT template. Furthermore, it possessed highly exposed M-N 4 metallic active sites and unique periodic porosity, thereby effectively addressing the key technical issue of room-temperature sensing for the respiration sensor. Meanwhile, the introduction of a pencil-drawing approach on common printing papers facilitates the inexpensive and simple manufacturing of the as-fabricated graphite IDE. Based on the above advantages, the MWCNTs@COF NiP-Ph respiration sensor had the characteristics of wide detection range (1-500 ppm), low detection limit (30 ppb), acceptable flexibility for toluene, and rapid response/recovery time (32 s/116 s). These advancements facilitated the integration of the respiration sensor into surgical masks and clothes with maximum functionality at a minimized size and weight. Moreover, the primary internal mechanism of COF NiP-Ph for this efficient toluene detection was investigated through in situ FTIR spectra, thereby directly elucidating that the chemisorption interaction of oxygen modulated the depletion layers, resulting in alterations in sensor resistance upon exposure to the target gas. The encouraging results revealed the feasibility of employing a paper-sensing system as a wearable platform in green electronics.

With the advancement of nanotechnology, various types of nanomaterials have been integrated into electrochemical immunoelectrodes to enhance their performance. Among these, MXene stands out as a promising candidate due to its high electron transfer capacity and abundant surface chemical groups. However, the improvement in electrode performance is often hindered by the self-restacking and agglomeration of MXene. To address this issue, multi-walled carbon nanotubes (MWCNTs) were selected to form composites with MXene. Subsequently, a label-free immunosensor, BSA/Ab/AuNPs/MXene-MWCNTs-Nafion/ITO, was fabricated for specific detection of carcinoembryonic antigen (CEA), a widely used tumor marker. The results demonstrated that the incorporation of MWCNTs can effectively prevent the self-stacking of MXene. Moreover, the composites enhanced the loading of gold nanoparticles (AuNPs) to connect the antibodies, thereby improving electronic transmission signals and sensitivity. The sensor exhibited excellent analytical performance towards CEA with a wide linear range (0.050 to 200 ng mL -1 ) and a low limit of detection of 0.015 ng mL -1 (S/N = 3). The possibility of it being applied in clinical trials was verified by using ELISA and differential pulse voltammetry (DPV) assays to detect CEA in serum samples. The recoveries ranged from 95.34 to 102.09% with relative standard deviations (RSDs) below 5.00%. Furthermore, the sensor displayed satisfactory selectivity, repeatability, and stability. We hope the findings highlight promising prospects for advanced immunosensor development and alternative strategies in cancer diagnosis., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)

A sandwich electrochemical sensor was fabricated based on multi-walled carbon nanotubes/ordered mesoporous carbon/AuNP (MWCNTs/CMK-3/AuNP) nanocomposites and porous core-shell nanoparticles Au@PdNPs to achieve rapid and sensitive detection of AFB 1 in complex matrices. MWCNTs/CMK-3/AuNP nanocomposite, which was prepared by self-assembly method, served as a substrate material to increase the aptamer loading and improve the conductivity and electrocatalytic activity of the electrode for the first signal amplification. Then, Au@PdNPs, which were synthesized by one-pot aqueous phase method, were applied as nanocarriers loaded with plenty of capture probe antibody (Ab) and signal molecule toluidine blue (Tb) to form the Au@PdNPs-Ab-Tb bioconjugates for secondary signal amplification. The sensing system could still significantly improve the signal output intensity even in the presence of ultra-low concentration target compound due to the dual signal amplification of MWCNTs/CMK-3/AuNP nanocomposites and Au@PdNPs-Ab-Tb. The method exhibited high selectivity, low detection limit (9.13 fg/mL), and strong stability to differentiate AFB 1 from other mycotoxins. Furthermore, the sensor has been successfully applied to the quantitative determination of AFB 1 in corn, malt, and six herbs, which has potential applications in food safety, quality control, and environmental monitoring., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)

A unique method for determining chlorophyll content in microalgae is devised employing a gold interdigitated electrode (G-IDE) with a 10-µm gap, augmented by a nano-molecularly imprinted polymer (nano-MIP) and a titanium dioxide/multiwalled carbon nanotube (TiO 2 /MWCNT) nanocomposite. The nano-MIP, produced using chlorophyll template voids, successfully trapped chlorophyll, while the TiO 2 /MWCNT nanocomposite, synthesized by the sol-gel technique, exhibited a consistent distribution and anatase crystalline structure. The rebinding of procured chlorophyll powder, which was used as a template for nano-MIP synthesis, was identified with a high determination coefficient (R 2  = 0.9857). By combining the TiO 2 /MWCNT nanocomposite with nano-MIP, the G-IDE sensing method achieved a slightly better R 2 value of 0.9892 for detecting chlorophyll in microalgae. The presented G-IDE sensor showed a significant threefold enhancement in chlorophyll detection compared with commercially available chlorophyll powder. It had a detection limit of 0.917 mL (v/v) and a linear range that spanned from 10 -6  to 1 mL. The effectiveness of the sensor in detecting chlorophyll in microalgae was confirmed through validation of its repeatability and reusability., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)

The adsorption of a series of aromatic compounds from aqueous solution onto purified, free-standing single-walled carbon nanotube/graphene nanoplatelet hybrid papers is studied both experimentally and theoretically. Experimental data is obtained via changes in optical absorption spectra of the aqueous solutions and is used to extract all parameters required to implement a semi-empirical mass-transfer model. Agreement between experiment and theory is excellent and data from all compounds can be cast on a universal adsorption curve. Results indicate that the rate of adsorption and long-time capacity of many aromatic compounds on hybrid paper adsorbent significantly exceeds that of activated carbon by at least an order of magnitude. The combination of carbon nanotubes and graphene also promotes on the order of a 25% improvement in adsorption rates and capacities than either component alone. Hybrid nanocomposites show significant promise as adsorption materials used for environmental remediation efforts.

A novel approach to monitor Li levels in blood in decentralized (out of the lab) settings is presented. The approach uses a potentiometric cell fully made with filter paper as a support. Electrodes were built using carbon nanotubes ink to create a conductive path and a suitable polymeric membrane. Solid-state ion-selective electrodes for Li and a reference electrode were built and optimized. The results obtained on real samples of serum and whole blood are comparable with those obtained by conventional standard approaches. This platform shows an outstanding performance for the direct, fast and low-cost monitoring of Li levels in blood.

A new paper-based colorimetric immunosensor for the detection of carcinoembryonic antigen (CEA) was developed based on the intrinsic peroxidase activity of ZnFe2O4-multiwalled carbon nanotubes (ZnFe2O4@MWNTs). The immunosensor platform was prepared by depositing chitosan and porous gold onto filter paper and entrapping the primary antibodies (Ab1) onto the layers. Secondary antibodies (Ab2) were assembled on the surface of the functionalized ZnFe2O4@MWNTs. The immunosensor response was quantified as a color change resulting from ZnFe2O4@MWNTs catalyzing the oxidation of 3,3',5,5'-tetramethylbenzidine in the presence of H2O2. The catalytic performance of ZnFe2O4@MWNTs was higher than ZnFe2O4 due to the high electrical conductance of MWNTs, moreover, the electron communications between ZnFe2O4@MWNTs and substrates are electrically "wired". Detection was achieved by measuring the color change when the concentrations of CEA were different. The color change can be quantified with the naked eye but a digitalized picture can also be used to provide more sensitive comparison to a calibrated color scheme. This method was simple for CEA detection with a linear range from 0.005 to 30 ng mL(-1) and a detection limit of 2.6 pg mL(-1). Such an equipment-free immunoassay has great potential in resource-limited environments.

A simple and effective ratiometric fluorescence nanosensor for the selective detection of Cu(2+) has been developed by covalently connecting the carboxyl-modified red fluorescent cadmium telluride (CdTe) quantum dots (QDs) to the amino-functionalized blue fluorescent carbon nanodots (CDs). The sensor exhibits the dual-emissions peaked at 437 and 654 nm, under a single excitation wavelength of 340 nm. The red fluorescence can be selectively quenched by Cu(2+), while the blue fluorescence is a internal reference, resulting in a distinguishable fluorescence color change from pink to blue under a UV lamp. The detection limit of this highly sensitive ratiometric probe is as low as 0.36 nM, which is lower than the U.S. Environmental Protection Agency (EPA) defined limit (20 μM). Moreover, a paper-based sensor has been prepared by printing the hybrid carbon dots-quantum dots probe on a microporous membrane, which provides a convenient and simple approach for the visual detection of Cu(2+). Therefore, the as-synthesized probe shows great potential application for the determination of Cu(2+) in real samples.

The self-assembled nanostructures of carbon nanomaterials possess a damage-tolerable architecture crucial for the inherent mechanical properties at both micro- and macroscopic levels. Bone, or "natural composite," has been known to have superior energy dissipation and fracture resistance abilities due to its unique load-bearing hybrid structure. However, few approaches have emulated the desirable structure using carbon nanomaterials. In this paper, we present an approach in fabricating a hybrid composite paper based on graphene oxide (GO) and carbon nanotube (CNT) that mimicks the natural bone structure. The size-tuning strategy enables smaller GO sheets to have more cross-linking reactions with CNTs and be homogeneously incorporated into CNT-assembled paper, which is advantageous for effective stress transfer. The resultant hybrid composite film has enhanced mechanical strength, modulus, toughness, and even electrical conductivity compared to previously reported CNT-GO based composites. We further demonstrate the usefulness of the size-tuned GOs as the "stress transfer medium" by performing in situ Raman spectroscopy during the tensile test.

The increasing demands for portable, wearable, and implantable sensing devices have stimulated growing interest in innovative electrode materials. In this work, we have demonstrated that printing a conductive ink formulated by blending three-dimensional (3D) porous graphene-carbon nanotube (CNT) assembly with ionic liquid (IL) on two-dimensional (2D) graphene paper (GP), leads to a freestanding GP supported graphene-CNT-IL nanocomposite (graphene-CNT-IL/GP). The incorporation of highly conductive CNTs into graphene assembly effectively increases its surface area and improves its electrical and mechanical properties. The graphene-CNT-IL/GP, as freestanding and flexible substrates, allows for efficient loading of PtAu alloy nanoparticles by means of ultrasonic-electrochemical deposition. Owing to the synergistic effect of PtAu alloy nanoparticles, 3D porous graphene-CNT scaffold, IL binder and 2D flexible GP substrate, the resultant lightweight nanohybrid paper electrode exhibits excellent sensing performances in nonenzymatic electrochemical detection of glucose in terms of sensitivity, selectivity, reproducibility and mechanical properties., (Copyright © 2015 Elsevier B.V. All rights reserved.)