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Acoustic levitation, which allows contactless manipulation of micro-objects with ultrasounds, is a promising technique for spheroids formation and culture. This acoustofluidic technique favors cell-cell interactions, away from the walls of the chip, which leads to the spontaneous self-organization of cells. Using this approach, we generated spheroids of mesenchymal stromal cells, hepatic and endothelial cells, and showed that long-term culture of cells in acoustic levitation is feasible. We also demonstrated that this self-organization and its dynamics depended weakly on the acoustic parameters but were strongly dependent on the levitated cell type. Moreover, spheroid organization was modified by actin cytoskeleton inhibitors or calcium-mediated interaction inhibitors. Our results confirmed that acoustic levitation is a rising technique for fundamental research and biotechnological industrial application in the rapidly growing field of microphysiological systems. It allowed easily obtaining spheroids of specific and predictable shape and size, which could be cultivated over several days, without requiring hydrogels or extracellular matrix., (© 2024 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals LLC.)

Per- and polyfluoroalkyl substances (PFAS) are of increasing concern due to their pervasive nature, high persistence, and their impacts on human health and the environment. Many studies have attempted to assess the presence of PFAS along the water cycle, but few have analyzed rainwater PFAS content and its contribution to water contamination. The present study aims to improve knowledge by providing the first analysis of PFAS rainwater samples from France. A total of 52 PFAS were analyzed at nanogram per liter levels in rainwater samples collected in 14 locations in France using a cutting-edge liquid chromatography-tandem mass spectrometry (LC/MS/MS) method for multiresidue determination. Depending on the quantity of rainwater collected, the PFAS concentrations were analyzed either by direct injection or after solid-phase extraction (SPE), allowing to quantify 20 PFAS with a limit of quantification (LOQ) ≤ 100 ng/L and 52 PFAS with a LOQ ≤ 1 ng/L, respectively. For the five locations for which the collected samples were analyzed by direct injection, no PFAS could be detected (i.e., their concentrations in the samples were below the LOQs of the method). The samples from four locations out of the nine analyzed by SPE-LC/MS/MS show results above the method's LOQs for up to 10 PFAS. Among the quantified PFAS, three compounds (perfluorononanoic acid, perfluoroundecanoic acid, and perfluorohexanoic acid) have been found to be of most significance. These results bring out the presence of PFAS in rainwater samples in France, highlighting the need for PFAS environmental surveillance and risk assessment and the necessity of continuous improvement of existing analysis methods., (© 2023 Eurofins Hydrologie Est. Journal of Environmental Quality © 2023 American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America.)

To mitigate anthropogenic impacts on plant diversity in tropical montane grasslands, one of the most threatened ecosystems in Brazil, it will be essential to develop ex situ conservation strategies to preserve wild species. The lack of basic research on the seed storage behaviour of grassland species may, however, limit their use for reintroduction and restoration projects. We investigated seed storage behaviour at the community level by comparing the effects of cold-low RH (10 °C; 10% RH) and freezing-low RH (20 °C; 10% RH) conditions on seed viability, germination and dormancy of 47 species. Fresh seeds of 43% of the species showed primary dormancy. More than half of the species showed high seed survival responses (viability >60%) under both storage temperatures. Despite a variety of dormancy responses among the different species, the low RH storage conditions tested released dormancy for most species during 12- and 30-month storage times. Multivariate analysis of the best (freezing-low RH, 30 months) storage condition evidenced the formation of five distinct groups, three with species having high conservation potential in seed banks. Although further studies are needed to test dormancy-breaking treatments and improve seed conservation practices, this first approach to assessing seed banking techniques could contribute to demand for locally adapted seeds for ecological restoration projects in tropical montane grasslands., (© 2021 German Society for Plant Sciences and The Royal Botanical Society of the Netherlands.)