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1. [Genetic characterization of the Syr-Darya valley fever virus (SDVFV) (Picornaviridae, Cardiovirus) isolated from the blood of the patients and ticks Hyalomma as. asiaticum (Hyalomminae), Dermacentor daghestanicus (Rhipicephalinae) (Ixodidae) and Ornithodoros coniceps (Argasidae) in Kazakhstan and Turkmenistan].

Author

L'vov DK, Al'khovskiĭ SV, Shchelkanov MIu, Shchetinin AM, Deriabin PG, Gitel'man AK, Aristova VA, and Botikov AG

Subjects
Amino Acid Sequence, Animals, Argasidae virology, Birds virology, Humans, Ixodidae virology, Kazakhstan, Metagenome, Molecular Sequence Data, Picornaviridae pathogenicity, Picornaviridae Infections genetics, Turkmenistan, Genome, Viral, Phylogeny, Picornaviridae genetics, Picornaviridae Infections virology
Abstract

The Syr-Darya valley fever virus (SDVFV) was originally isolated from the blood of the patient with fever in the Kyzylorda province, Kazakhstan, in July 1973 and was classified to the Cardiovirus genus (fam. Picornaviridae). Later, SDVFV was isolated from the ticks Hyalomma as. asiaticum Schulze et Schlottke, 1929 (Hyalomminae) (1 strain) and Dermacentor daghestanicus Olenev, 1929 (Rhipicephalinae) (7 strains), collected in the floodplains of the Syr-Darya river and the Ili river. In this paper, complet genome of the SDVFV (strain LEIV-Tur2833) was sequenced using the next-generation sequencing approach (GenBank ID: KJ191558). It was demonstrated that, phylogenetically, the SDVFV is closely related closest to the Theiler's murine encephalomyelitis virus (TMEV) and Vilyuisk human encephalomyelitis virus (VMEV). The similarity of the SDVFV with VHEV and TMEV based on P1 region of the polyprotein-precursor (structural proteins VP1-VP4), reaches 75% and 91% for nucleotide sequences and 80% and 93% for putative amino acid sequences, respectively. For nonstructural proteins regions P2 (2A-2C) and P3 (3A-3D) similarity of SDVFV with TMEV and VHEV is 96%-98%.

Published
2014

2. [Isolation of the virus of Syr-Darya Valley fever].

Author

L'vov DK, Karimov SK, Kiriushchenko TV, Chun-Siun F, and Skvortsova TM

Subjects
Adult, Animals, Animals, Newborn, Antigens, Viral analysis, Brain pathology, Chlorocebus aethiops, Cricetinae, Humans, Kazakhstan, Male, Mesocricetus, Mice, Microscopy, Electron, Picornaviridae classification, Picornaviridae pathogenicity, Picornaviridae Infections microbiology, Picornaviridae Infections pathology, Serotyping, Ticks microbiology, Virus Cultivation, Picornaviridae isolation & purification
Abstract

In the course of studies on the ecological structure of acute febrile diseases in the season of activity of blood-sucking arthropods strains of a virus antigenically related to Sikhote-Alyñ virus were isolated from the blood of a patient and from Ixodid ticks. This paper presents the results of the study on the causative agent and the clinical picture of the disease caused by this virus. The virus was found to be a new one for science; its appurtenance to the family Picornaviridae, genus Cardiovirus, the antigenic group of encephalomyocarditis has been determined. The virus has been designated "Syr-Darya Valley fever virus" by the area of its isolation.

Published
1984