1. Alanyl-glutamine ameliorates lipopolysaccharide-induced inflammation and barrier function injury in bovine jejunum epithelial cells
- Author
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Zhang, Xianglun, Tan, Xiuwen, Liu, Yifan, You, Wei, Liu, Guifen, Liu, Xiaomu, Jin, Qing, Wei, Chen, Wan, Fachun, and Zhao, Hongbo
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Mitogens -- Analysis ,Inflammation -- Analysis ,Glutamine -- Analysis ,RNA -- Analysis ,Interleukins -- Analysis ,Biological sciences - Abstract
The aim of this study was to investigate the effects of alanyl-glutamine (Ala-Gln) on the regulation of lipopolysaccharide (LPS)-induced inflammation and barrier function in bovine jejunum epithelial cells (BJECs). BJECs were exposed (or not) to 1 [micro]g/mL LPS for 24 h to generate a pro-inflammatory model. The cells were then treated with different concentrations of Ala-Gln (0.25, 0.5, 1.0, 2.0, or 4.0 mmol/L) to detect any regulatory effects on the inflammation and barrier function of BJECs. LPS decreased cell viability and enhanced the production of the pro-inflammatory cytokines interleukin (IL)-6 and IL-8. LPS induced inflammation and damaged the barrier function of BJECs, as evidenced by up-regulated mRNA and protein expression of inflammatory factors and down-regulated expression of tight junction proteins. Conversely, Ala-Gln rescued the decrease in cell viability and prevented the accumulation of ILs after LPS exposure by reducing the mRNA and protein expression levels of inflammatory factors. In addition, Ala-Gln induced the mRNA and protein expression of multiple tight junction proteins, and thus reconstituted the barrier function of BJECs. In conclusion, Ala-Gln attenuates injury from inflammation and repairs damaged intestinal barrier induced with LPS, suggesting its potential as a therapeutic agent against intestinal inflammation in mammals. Key words: alanyl-glutamine, bovine intestinal cell, lipopolysaccharide, inflammation, intestinal barrier function. L'objectif de cette etude consistait a examiner les effets de l'alanyl-glutamine (Ala-Gln) sur la regulation de l'inflammation provoquee par le lipopolysaccharide (LPS) et sur la fonction de barriere des cellules epitheliales jejunales bovines (CEJB). Les CEJB ont ete exposees ou non a 1 [micro]g/mL de LPS pendant 24 h afin de produire un modele pro-inflammatoire. Les cellules ont ensuite ete traitees avec differentes concentrations d'Ala-Gln (0,25, 0,5,1,0, 2,0 ou 4,0 mmol/L) afin de detecter un possible effet regulateur sur l'inflammation et la fonction de barriere des CEJB. Le LPS diminuait la viabilite cellulaire et accroissait la production des cytokines pro-inflammatoires interleukine-6 (IL-6) et IL-8. Le LPS provoquait une inflammation et endommageait la fonction de barriere des CEJB, comme le demontraient la regulation a la hausse de l'ARNm et l'expression proteique de facteurs inflammatoires ainsi que la regulation a la baisse de l'expression des proteines des jonctions serrees. A l'inverse, l'Ala-Gln contrecarrait la diminution de la viabilite cellulaire et prevenait l'accumulation d'IL a la suite d'une exposition au LPS en reduisant les niveaux d'ARNm et d'expression proteique de facteurs inflammatoires. De plus, l'Ala-Gln induisait l'expression plusieurs proteines des jonctions serrees et de leur ARNm et ainsi, reconstituait la fonction de barriere de CEJB. En conclusion, l'Ala-Gln pouvait attenuer le dommage inflammatoire et reparer la barriere intestinale endommagee par le LPS, suggerant qu'il ait le potentiel d'agir comme d'agent therapeutique contre l'inflammation intestinale chez les mammiferes. [Traduit par la Redaction] Mots-cles: alanyl-glutamine, cellules intestinales bovines, lipopolysaccharide, inflammation, fonction de barriere intestinale., Introduction The small intestine is the main site of nutrient digestion and absorption in the body. In addition, the small intestine epithelium also serves as a barrier to foreign pathogens [...]
- Published
- 2019
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