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Your search keyword '"Tourlousse, Dieter M."' showing total 3 results
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3 results on '"Tourlousse, Dieter M."'

1. Influence of dangling ends and surface-proximal tails of targets on probe-target duplex formation in 16S rRNA gene-based diagnostic arrays

Author

Stedtfeld, Robert D., Wick, Lukas M., Baushke, Samuel W., Tourlousse, Dieter M., Herzog, Amanda B., Yongmei Xia, Rouillard, Jean Marie, Klappenbach, Joel A., Cole, James R., Gulari, Erdogan, Tiedje, James M., and Hashsham, Syed A.

Subjects
Ribosomal RNA -- Research, Pathogenic microorganisms -- Genetic aspects, Pathogenic microorganisms -- Research, Biological sciences
Abstract

The effect of danglings ends and surface-promixal tails of gene targets on probe-target duplex formation is evaluated by using an oligonucleotide microarray containing 18-mer probes corresponding to the 16S rRNA genes of 10 waterborne pathogens and a number of synthetic and PCR-applied gene targets. The study has highlighted the requirement of a careful target preparation and evaluation of signal intensities for diagnostic arrays by using 16S rRNA and other gene targets because of the potential for target interaction with a complex background.

Published
2007

2. Development and experimental validation of a predictive threshold cycle equation for quantification of virulence and marker genes by high-throughput nanoliter-volume PCR on the OpenArray platform

Author

Stedfeld, Robert D., Baushke, Samuel W., Tourlousse, Dieter M., Miller, Sarah M., Stedfeld, Tiffany M., Gulari, Erdogan, Tiedje, James M., and Hashsham, Syed A.

Subjects
DNA testing -- Analysis, Pathogenic microorganisms -- Research, Polymerase chain reaction -- Evaluation, Virulence (Microbiology) -- Research, Biological sciences
Abstract

Nanoliter-volume BioTrove OpenArray platform was used for examining the capacity of quantitative PCR (QPCR) for highly parallel diagnostics of human pathogens. Findings reveal a high correlation between the predicted and experimental threshold cycle.

Published
2008

3. In situ-synthesized virulence and marker gene biochip for detection of bacterial pathogens in water

Author

Miller, Sarah M., Tourlousse, Dieter M., Stedtfeld, Robert D., Baushke, Samuel W., Herzog, Amanda B., Wick, Lukas M., Rouillard, Jean Marie, Gulari, Erdogan, Tiedje, James M., and Hashsham, Syed A.

Subjects
Bacteria, Pathogenic -- Physiological aspects, Bacteria, Pathogenic -- Genetic aspects, Virulence (Microbiology) -- Research, Biochips -- Usage, Polymerase chain reaction -- Analysis, DNA microarrays -- Analysis, Biological sciences
Abstract

The development and validation of an in situ-synthesized virulence and marker genes (VMG) biochip for detection of microbial pathogens is reported. The biochip can detect bacterial pathogens when parallel and specific detection is required.

Published
2008

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