1. Markers of glycemic control in the mouse: comparisons of 6-h- and overnight-fasted blood glucoses to Hb [A.sub.1c]
- Author
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Han, Byoung Geun, Hao, Chuan-Ming, Tchekneva, Elena E., Wang, Ying-Ying, Lee, Chieh Allen, Ebrahim, Benyamin, Harris, Raymond C., Kern, Timothy S., Wasserman, David H., Breyer, Matthew D., and Qi, Zhonghua
- Subjects
Glycosylated hemoglobin -- Properties ,Glycemic index -- Measurement ,Biological markers -- Identification and classification ,Physiological research ,Biological sciences - Abstract
The present studies examined the relationship between fasting blood glucose and Hb [A.sub.1c] in C57BL/6J, DBA/2J, and KK/H1J mice with and without diabetes mellitus. Daily averaged blood glucose levels based on continuous glucose monitoring and effects of 6-h vs. overnight fasting on blood glucose were determined. Daily averaged blood glucose levels were highly correlated with Hb [A.sub.1c], as determined with a hand-held automated device using an immunodetection method. [R.sup.2] values were 0.90, 0.95, and 0.99 in KK/HIJ, C57BL/6J, and DBA/2J, respectively. Six-hour fasting blood glucose correlated more closely with the level of daily averaged blood glucose and with Hb [[A.sub.1c] than did blood glucose following an overnight fast. To validate the immunoassay-determined Hb [A.sub.1c], we also measured total glycosylated hemoglobin using boronate HPLC. Hb [A.sub.1c] values correlated well with total glycosylated hemoglobin in all three strains but were relatively lower than total glycosylated hemoglobin in diabetic DBA/2J mice. These results show that 6-h fasting glucose provides a superior index of glycemic control and correlates more closely with Hb [A.sub.1c] than overnight-fasted blood glucose in these strains of mice. glycosylated hemoglobin; methodology; mice
- Published
- 2008