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2 results on '"Morag, Ely"'

1. Expression, purification, and characterization of the cellulose-binding domain of the scaffoldin subunit from the cellulosome of the Clostridium thermocellum

Author

Morag, Ely, Lapidot, Aviva, Govorko, Dmitry, Lamed, Raphael, Wilchek, Meir, Bayer, Edward A., and Shoham, Yuval

Subjects
Clostridium -- Genetic aspects, Binding sites (Biochemistry) -- Research, Biological sciences
Abstract

The cellulose-binding domain (CBD) present on a noncatalytic scaffoldin subunit of the cellulosome in Clostridium thermocellum is similar in structure, substrate affinity and binding capacity to the CBD present in C. cellulovorans. However the polyclonal antibodies produced against the CBD of C. thermocellum do not act against the CBD of C. cellulovorans possibly because the exposed epitopes in the two CBD's are different. Chemical changes in the cysteine of the CBD do not affect the CBD's ability to bind cellulose.

Published
1995

2. Regulation of the cellulosomal celS (cel48A) gene of Clostridium thermocellum is growth rate dependent

Author

Dror, Tali W., Morag, Ely, Rolider, Adi, Bayer, Edward A., Lamed, Raphael, and Shoham, Yuval

Subjects
Microbiological synthesis -- Genetic aspects, Microbiological synthesis -- Physiological aspects, Gene expression -- Physiological aspects, Bacterial growth -- Physiological aspects, Biological sciences
Abstract

Results show that the cellulosomal gene celS is regulated by growth rate in Clostridium thermocellum. The gene encoded product CelS exhibits exoglucanase activity. Data indicae that the amount of CelS produced by cellobiose-grown cultures is lower than that of cellulose-grown cultures.

Published
2003

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