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The effective use of targeted therapy is highly dependent on the identification of responder patient populations. Loss of FBW7, which encodes a tumour-suppressor protein, is frequently found in various types of human cancer, including breast cancer, colon cancer (1) and T-cell acute lymphoblastic leukaemia (T-ALL) (2). In line with these genomic data, engineered deletion of Fbw7 in mouse T cells results in T-ALL (3-5), validating FBW7 as a T-ALL tumour suppressor. Determining the precise molecular mechanisms by which FBW7 exerts antitumour activity is an area of intensive investigation. These mechanisms are thought to relate in part to FBW7-mediated destruction of key proteins relevant to cancer, including Jun (6), Myc (7), cyclin E (8) and notch 1 (ref. 9), all of which have oncoprotein activity and are overexpressed in various human cancers, including leukaemia. In addition to accelerating cell growth (10), overexpression of Jun, Myc or notch 1 can also induce programmed cell death (11). Thus, considerable uncertainty surrounds how FBW7-deficient cells evade cell death in the setting of upregulated Jun, Mbc and/or notch 1. Here we show that the E3 ubiquitin ligase [SCF.sup.FBW7] (a SKP1-cullin-1-F-box complex that contains FBW7 as the F-box protein) governs cellular apoptosis by targeting MCL1, a pro-survival BCL2 family member, for ubiquitylation and destruction in a manner that depends on phosphorylation by glycogen synthase kinase 3. Human T-ALL cell lines showed a close relationship between FBW7 loss and MCL1 overexpression. Correspondingly, T-ALL cell lines with defective FBW7 are particularly sensitive to the multi-kinase inhibitor sorafenib but resistant to the BCL2 antagonist ABT-737. On the genetic level, FBW7 reconstitution or MCL1 depletion restores sensitivity to ABT-737, establishing MCL1 as a therapeutically relevant bypass survival mechanism that enables FBW7-deficient cells to evade apoptosis. Therefore, our work provides insight into the molecular mechanism of direct tumour suppression by FBW7 and has implications for the targeted treatment of patients with FBW7-deficient T-ALL., MCL1 is frequently overexpressed in various leukaemias through mechanisms that are not fully understood (12). MCL1 is distinct from other BCL2 family members in its extremely unstable nature (13), which [...]

The management of research and development activities in both the public and private sectors is compared to the management of other organizational areas. A survey of 448 managers indicates that research and development managers view their roles in much the same manner as other managers. In addition, the research indicates that managerial roles are assigned similar levels of importance by managers in both private industry and governmental work.