1. Whole Genome Sequencing in Advanced Lung Cancer can be Performed Using Diff-Quik Cytology Smears Derived from Endobronchial Ultrasound, Transbronchial Needle Aspiration (EBUS TBNA)
- Author
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Fielding, David, Dalley, Andrew J., Singh, Mahendra, Nandakumar, Lakshmy, Lakis, Vanessa, Chittoory, Haarika, and Fairbairn, David
- Subjects
Nucleotide sequencing -- Analysis -- Technology application -- Genetic aspects ,Medical research -- Analysis -- Technology application ,Medicine, Experimental -- Analysis -- Technology application ,Genomics -- Analysis -- Genetic aspects -- Technology application ,Genetic research -- Analysis -- Genetic aspects -- Technology application ,DNA sequencing -- Analysis -- Technology application -- Genetic aspects ,Genomes -- Technology application -- Genetic aspects -- Analysis ,Cancer -- Genetic aspects ,Lung cancer -- Genetic aspects ,Technology application ,Health - Abstract
Introduction Maximising alternative sample types for genomics in advanced lung cancer is important because bronchoscopic samples may sometimes be insufficient for this purpose. Further, the clinical applications of comprehensive molecular analysis such as whole genome sequencing (WGS) are rapidly developing. Diff-Quik cytology smears from EBUS TBNA is an alternative source of DNA, but its feasibility for WGS has not been previously demonstrated. Methods Diff-Quik smears were collected along with research cell pellets. Results Tumour content of smears were compared to research cell pellets from 42 patients, which showed good correlation (Spearman correlation 0.85, P 1500 ng in 7 out of 8 smears. Conclusions WGS of commonly collected Diff-Quik slides is feasible and their DNA yield can be predicted., Author(s): David Fielding [sup.1] [sup.2], Andrew J. Dalley [sup.2], Mahendra Singh [sup.2] [sup.3], Lakshmy Nandakumar [sup.3], Vanessa Lakis [sup.4], Haarika Chittoory [sup.2], David Fairbairn [sup.3], Kaltin Ferguson [sup.2], Farzad Bashirzadeh [...]
- Published
- 2023
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