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1. Kinetics and thermodynamics of RRF, EF-G, and thiostrepton interaction on the Escherichia coli ribosome

Author

Hyuk-Soo Seo, Kiel, Michael, Pan, Dongli, Raj, V. Samuel, Kaji, Akira, and Cooprman, Barry S.

Subjects
Escherichia coli -- Research, Ribosomes -- Research, Thermodynamics -- Analysis, Biological sciences, Chemistry
Abstract

Equilibrium and rapid kinetic measurements permitting formulation of a minimal kinetic scheme, which accounts for RRF and EF-G interaction on the Escherichia coli ribosome, are presented. Ribosome recycling factor (RRF) and elongation factor-G (EF-G) are essential for recycling of bacterial ribosome. Action of the thiostrepton interaction slows the initial binding process of EF-G complex and its findings are quite relevant for the mechanism of post-termination complex

Published
2004

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