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The bacterial plasmid pEMT8 contains the tfdA gene that encodes the 2,4-dichlorophenoxyacetic acid (2,4-D):alpha-ketoglutaric acid dioxygenase (TfdA) involved in 2,4-D degradation. The tfdA gene is 94% similar to that of the Burkholderia sp. strain RASC and has somewhat less similarity to that present on the Alcaligenes eutrophus pJP4 plasmid. No genes similar to the tfdB and tfdCDEF are present on pEMT8. The pEMT8 plasmid is captured by genetic complementation of transconjugates of A. eutrophus containing a pJP4 plasmid lacking the tfdA gene and bacteria from the soil.