79 results on '"Drosten, Christian"'
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2. Divergent Genotype of Hepatitis A Virus in Alpacas, Bolivia, 2019
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Veith, Talitha, Beltran-Saavedra, L. Fabian, Bleicker, Tobias, Schmidt, Marie Luisa, Mollericona, Jose L., Grutzmacher, Kim, Wallace, Rob, Drexler, Jan Felix, Walzer, Christian, Jones, Terry C., Drosten, Christian, and Corman, Victor Max
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Hepatitis A -- Risk factors -- Causes of ,Alpaca -- Health aspects ,Genotype -- Research ,Hepatitis A virus -- Identification and classification -- Genetic aspects -- Distribution ,Virus research ,Company distribution practices ,Health - Abstract
Hepatitis A virus (HAV) causes acute hepatitis in humans worldwide; [approximately equal to] 159 million infections and [approximately equal to] 39,000 deaths were associated with HAV in 2019 (1), despite [...]
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- 2023
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3. US National Institutes of Health Prioritization of SARS-CoV-2 Variants
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Turner, Sam, Alisoltani, Arghavan, Bratt, Debbie, Cohen-Lavi, Liel, Dearlove, Bethany L., Drosten, Christian, Fischer, Will M., Fouchier, Ron A.M., Gonzalez-Reiche, Ana Silvia, Jaroszewski, Lukasz, Khalil, Zain, LeGresley, Eric, Johnson, Marc, Jones, Terry C., Muhlemann, Barbara, O'Connor, David, Sedova, Mayya, Shukla, Maulik, Theiler, James, Wallace, Zachary S., Yoon, Hyejin, Zhang, Yun, van Bakel, Harm, Degrace, Marciela M., Ghedin, Elodie, Godzik, Adam, Hertz, Tomer, Korber, Bette, Lemieux, Jacob, Niewiadomska, Anna M., Post, Diane J., Rolland, Morgane, Scheuermann, Richard, and Smith, Derek J.
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Values -- Analysis ,Microbial mutation -- Health aspects ,Epidemiology -- Methods ,Health - Abstract
As part of the US National Institutes of Health (NIH) National Institute of Allergy and Infectious Diseases (NIAID) SARS-CoV-2 Assessment of Viral Evolution (SAVE) effort to combat the SARS-CoV-2 pandemic, [...]
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- 2023
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4. Effectiveness of Naturally Acquired and Vaccine-Induced Immune Responses to SARS-CoV-2 Mu Variant
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de Oliveira-Filho, Edmilson F., Rincon-Orozco, Bladimiro, Jones-Cifuentes, Natalia, Pena-Lopez, Brigitte, Muhlemann, Barbara, Drosten, Christian, Moreira-Soto, Andres, and Drexler, Jan Felix
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Immune response -- Evaluation ,Company distribution practices ,Health - Abstract
Diverse SARS-CoV-2 variants have arisen during the pandemic. As of May 4, 2022, there had been 2 recognized variants of concern (VOC), Delta and Omicron, in addition to earlier emerging [...]
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- 2022
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5. Emergence of SARS-CoV-2 Delta Variant, Benin, May--July 2021
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Yadouleton, Anges, Sander, Anna-Lena, Adewumi, Praise, de Oliveira Filho, Edmilson F., Tchibozo, Carine, Hounkanrin, Gildas, Rene, Keke K., Ange, Dossou, Kohoun, Rodrigue K., Nari, Ramalia Chabi, Salifou, Sourakatou, Saizonou, Raoul, Kakai, Clement G., Bedie, Sonia V., Onifade, Fattah Al, Nagel, Michael, Aissi, Melchior A. Joel, Akogbeto, Petas, Drosten, Christian, Wulf, Ben, Moreira-Soto, Andres, Djingarey, Mamoudou Harouna, Hounkpatin, Benjamin, and Drexler, Jan Felix
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Epidemics -- Statistics -- Causes of -- Benin ,Health - Abstract
Numerous genetic variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have emerged globally since the start of the coronavirus disease (COVID-19) pandemic (https://cov-lineages.org). By September 2021, the World Health [...]
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- 2022
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6. Mutations Associated with SARS-CoV-2 Variants of Concern, Benin, Early 2021
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Sander, Anna-Lena, Yadouleton, Anges, de Oliveira Filho, Edmilson F., Tchibozo, Carine, Hounkanrin, Gildas, Badou, Yvette, Adewumi, Praise, Rene, Keke K., Ange, Dossou, Sourakatou, Salifou, Sedjro, Eclou, Aissi, Melchior A. Joel, Fidelia, Hinson, Djingarey, Mamoudou Harouna, Nagel, Michael, Jo, Wendy Karen, Moreira-Soto, Andres, Drosten, Christian, Landt, Olfert, Corman, Victor Max, Hounkpatin, Benjamin, and Drexler, Jan Felix
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Gene mutations -- Research ,Epidemics -- Causes of -- Benin ,Virus research ,Health - Abstract
Genomic surveillance is key to elucidate coronavirus disease (COVID-19) transmission chains and to monitor emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants associated with partial or complete immune escape [...]
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- 2021
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7. Delayed Antibody and T-Cell Response to BNT162b2 Vaccination in the Elderly, Germany
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Schwarz, Tatjana, Tober-Lau, Pinkus, Hillus, David, Helbig, Elisa T., Lippert, Lena J., Thibeault, Charlotte, Koch, Willi, Landgraf, Irmgard, Michel, Janine, Bergfeld, Leon, Niemeyer, Daniela, Muhlemann, Barbara, Conrad, Claudia, Dang-Heine, Chantip, Kasper, Stefanie, Munn, Friederike, Kappert, Kai, Nitsche, Andreas, Tauber, Rudolf, Schmidt, Sein, Kopankiewicz, Piotr, Bias, Harald, Seybold, Joachim, von Kalle, Christof, Jones, Terry C., Suttorp, Norbert, Drosten, Christian, Sander, Leif Erik, Corman, Victor M., and Kurth, Florian
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Antigen-antibody reactions -- Health aspects ,Aged patients -- Care and treatment ,T cells -- Health aspects ,Immune response -- Health aspects ,Health - Abstract
We detected delayed and reduced antibody and T-cell responses after BNT162b2 vaccination in 71 elderly persons (median age 81 years) compared with 123 healthcare workers (median age 34 years) in [...]
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- 2021
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8. Outbreak of SARS-CoV-2 B.1.1.7 Lineage after Vaccination in Long-Term Care Facility, Germany, February-March 2021
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Tober-Lau, Pinkus, Schwarz, Tatjana, Hillus, David, Spieckermann, Jana, Helbig, Elisa T., Lippert, Lena J., Thibeault, Charlotte, Koch, Willi, Bergfeld, Leon, Niemeyer, Daniela, Muhlemann, Barbara, Conrad, Claudia, Kasper, Stefanie, Munn, Friederike, Kunitz, Frank, Jones, Terry C., Suttorp, Norbert, Drosten, Christian, Sander, Leif Erik, Kurth, Florian, and Corman, Victor M.
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Vaccination -- Patient outcomes ,Epidemics -- Risk factors -- Demographic aspects -- Control -- Germany ,Long-term care facilities -- Management -- Safety and security measures ,Company business management ,Health - Abstract
One week after second vaccinations were administered, an outbreak of B.1.1.7 lineage severe acute respiratory syndrome coronavirus 2 infections occurred in a long-term care facility in Berlin, Germany, affecting 16/20 [...]
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- 2021
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9. Severe Acute Respiratory Syndrome Coronavirus 2 Outbreak Related to a Nightclub, Germany, 2020
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Muller, Nadine, Kunze, Mareike, Steitz, Fabienne, Saad, Neil J., Muhlemann, Barbara, Beheim-Schwarzbach, Jorn I., Schneider, Julia, Drosten, Christian, Murajda, Lukas, Kochs, Sandra, Ruscher, Claudia, Walter, Jan, Zeitlmann, Nadine, and Corman, Victor M.
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Epidemics -- Control -- Germany ,Safety regulations -- Evaluation ,Sentinel health events -- Methods ,Nightclubs -- Health aspects ,Health - Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) superspreading events are particularly linked to indoor settings, such as religious venues (1), restaurants (2), and bars or nightclubs (3-6). To provide further [...]
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- 2021
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10. Hepatitis E Virus Genotype 7 RNA and Antibody Kinetics in Naturally Infected Dromedary Calves, United Arab Emirates
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Corman, Victor M., Nagy, Peter, Ostermann, Stefanie, Arloth, Jacqueline, Liljander, Anne, Barua, Rajib, Gupta, Aungshuman Das, Hakimuddin, Fatima, Juhasz, Judit, Wernery, Ulrich, and Drosten, Christian
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Hepatitis E -- Genetic aspects ,Cattle -- Genetic aspects ,Infection -- Genetic aspects ,Antibodies -- Genetic aspects ,Food contamination ,RNA -- Genetic aspects ,Swine -- Genetic aspects ,Health - Abstract
Infection with hepatitis E viruses (HEVs) is one of the major causes of acute hepatitis in humans (1). Most HEV strains infecting humans belong to the virus species Orthohepevirus A [...]
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- 2020
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11. Inactivation of Severe Acute Respiratory Syndrome Coronavirus 2 by WHO-Recommended Hand Rub Formulations and Alcohols
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Kratzel, Annika, Todt, Daniel, Vkovski, Philip, Steiner, Silvio, Gultom, Mitra, Thao, Tran Thi Nhu, Ebert, Nadine, Holwerda, Melle, Steinmann, Jorg, Niemeyer, Daniela, Dijkman, Ronald, Kampf, Gunter, Drosten, Christian, Steinmann, Eike, Thiel, Volker, and Pfaender, Stephanie
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Severe acute respiratory syndrome -- Health aspects ,Alcohols -- Health aspects ,Coronaviruses -- Health aspects ,COVID-19 -- Health aspects ,Health - Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the third highly pathogenic human coronavirus to cross the species barrier into the human population during the past 20 years (2-3). SARS-CoV-2 [...]
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- 2020
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12. Severe Acute Respiratory Syndrome Coronavirus 2-Specific Antibody Responses in Coronavirus Disease Patients
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Okba, Nisreen M.A., Muller, Marcel A., Li, Wentao, Wang, Chunyan, GeurtsvanKessel, Corine H., Corman, Victor M., Lamers, Mart M., Sikkema, Reina S., de Bruin, Erwin, Chandler, Felicity D., Yazdanpanah, Yazdan, Hingrat, Quentin Le, Descamps, Diane, Houhou-Fidouh, Nadhira, Reusken, Chantal B.E.M., Bosch, Berend-Jan, Drosten, Christian, Koopmans, Marion P.G., and Haagmans, Bart L.
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Severe acute respiratory syndrome -- Health aspects ,Enzyme-linked immunosorbent assay -- Health aspects ,Coronaviruses -- Health aspects ,Medical research -- Health aspects ,Immunoglobulin A -- Health aspects ,Immunoglobulin G -- Health aspects ,COVID-19 -- Health aspects ,Health - Abstract
In December 2019, a new coronavirus emerged in China and caused an acute respiratory disease now known as coronavirus disease 2019 (COVID-19) (1). The virus was identified to be a [...]
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- 2020
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13. Comparison of Serologic Assays for Middle East Respiratory Syndrome Coronavirus
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Harvey, Ruth, Mattiuzzo, Giada, Hassall, Mark, Sieberg, Andrea, Muller, Marcel A., Drosten, Christian, Rigsby, Peter, and Oxenford, Christopher J.
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Algorithms -- Comparative analysis -- Health aspects ,Middle East respiratory syndrome -- Comparative analysis -- Health aspects ,Beef cattle ,Algorithm ,Health ,World Health Organization - Abstract
Since the emergence of Middle East respiratory syndrome coronavirus (MERS-CoV) in 2012 (1), more than 2,250 laboratory-confirmed cases have been reported to the World Health Organization (WHO); approximately one third [...]
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- 2019
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14. Sand Fly-Associated Phlebovirus with Evidence of Neutralizing Antibodies in Humans, Kenya
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Tchouassi, David P., Marklewitz, Marco, Chepkorir, Edith, Zirkel, Florian, Agha, Sheila B., Tigoi, Caroline C., Koskei, Edith, Drosten, Christian, Borgemeister, Christian, Torto, Baldwyn, Junglen, Sandra, and Sang, Rosemary
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Mosquitoes -- Analysis -- Health aspects ,Amino acids -- Analysis -- Health aspects ,Infection -- Analysis -- Health aspects ,Genomes -- Analysis -- Health aspects ,Antibodies -- Analysis -- Health aspects ,Genomics -- Analysis -- Health aspects ,Swine -- Analysis -- Health aspects ,Rural areas ,Beef cattle ,Wildlife ,Newborn infants ,Novels ,Health - Abstract
Disease outbreaks caused by Zika, dengue, yellow fever, chikungunya, and Rift Valley fever viruses illustrate the threat posed by arthropodborne viruses (arboviruses), which affect millions of patients worldwide each year [...]
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- 2019
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15. Differential Shedding and Antibody Kinetics of Zika and Chikungunya Viruses, Brazil
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Bozza, Fernando A., Moreira-Soto, Andres, Rockstroh, Alexandra, Fischer, Carlo, Nascimento, Alessandra D., Calheiros, Andrea S., Drosten, Christian, Bozza, Patricia T., Souza, Thiago Moreno L., Ulbert, Sebastian, and Drexler, Jan Felix
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Dengue virus -- Health aspects ,Zika virus -- Health aspects ,Infection -- Health aspects ,Antibodies -- Health aspects ,Immune response ,Recurrence (Disease) ,Medical tests ,Antigens ,Health - Abstract
In 2013, Zika virus and chikungunya virus (CHIKV) emerged in Latin America (1,2). Their overlapping symptoms challenge accurate diagnosis on the basis of clinical manifestations (3). Direct Zika virus and [...]
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- 2019
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16. Results of the CAPSID randomized trial for high-dose convalescent plasma in patients with severe COVID-19
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Korper, Sixten, Weiss, Manfred, Zickler, Daniel, Wiesmann, Thomas, Zacharowski, Kai, Corman, Victor M., Gruner, Beate, Ernst, Lucas, Spieth, Peter, Lepper, Philipp M., Bentz, Martin, Zinn, Sebastian, Paul, Gregor, Kalbhenn, Johannes, Dollinger, Matthias M., Rosenberger, Peter, Kirschning, Thomas, Thiele, Thomas, Appl, Thomas, Mayer, Benjamin, Schmidt, Michael, Drosten, Christian, Wulf, Hinnerk, Kruse, Jan Matthias, Jungwirth, Bettina, Seifried, Erhard, and Schrezenmeier, Hubert
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Hospital patients -- Care and treatment ,Health care industry - Abstract
BACKGROUND. COVID-19 convalescent plasma (CCP) has been considered a treatment option for COVID-19. This trial assessed the efficacy of a neutralizing antibody containing high-dose CCP in hospitalized adults with COVID-19 requiring respiratory support or intensive care treatment. METHODS. Patients (n = 105) were randomized 1:1 to either receive standard treatment and 3 units of CCP or standard treatment alone. Control group patients with progress on day 14 could cross over to the CCP group. The primary outcome was a dichotomous composite outcome of survival and no longer fulfilling criteria for severe COVID-19 on day 21. RESULTS. The primary outcome occurred in 43.4% of patients in the CCP group and 32.7% in the control group (P = 0.32). The median time to clinical improvement was 26 days in the CCP group and 66 days in the control group (P = 0.27). The median time to discharge from the hospital was 31 days in the CCP group and 51 days in the control group (P = 0.24). In the subgroup that received a higher cumulative amount of neutralizing antibodies, the primary outcome occurred in 56.0% of the patients (vs. 32.1%), with significantly shorter intervals to clinical improvement (20 vs. 66 days, P< 0.05) and to hospital discharge (21 vs. 51 days, P = 0.03) and better survival (day-60 probability of survival 91.6% vs. 68.1%, P = 0.02) in comparison with the control group. CONCLUSION. CCP added to standard treatment was not associated with a significant improvement in the primary and secondary outcomes. A predefined subgroup analysis showed a significant benefit of CCP among patients who received a larger amount of neutralizing antibodies. TRIAL REGISTRATION. ClinicalTrials.gov NCT04433910. FUNDING. Bundesministerium fur Gesundheit (German Federal Ministry of Health): ZMVI1-2520COR802., Introduction COVID-19 convalescent plasma (CCP) from patients who have recovered from a SARS-CoV-2 infection has become one of the treatment options for severe COVID-19 (1, 2). It has been broadly [...]
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- 2021
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17. External Quality Assessment for Zika Virus Molecular Diagnostic Testing, Brazil
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Fischer, Carlo, Pedroso, Celia, Mendrone, Alfredo, Jr., de Filippis, Ana Maria Bispo, Vallinoto, Antonio Carlos Rosario, Ribeiro, Bergmann Morais, Durigon, Edison Luiz, Marques, Ernesto T.A., Jr., Campos, Gubio S., Viana, Isabelle F.T., Levi, Jose Eduardo, Scarpelli, Luciano Cesar, Nogueira, Mauricio Lacerda, de Souza Bastos, Michele, Souza, Nathalia C. Santiago, Khouri, Ricardo, Lira, Sanny M. Costa, Komninakis, Shirley Vasconcelos, Baronti, Cecile, Charrel, Remi N., Kummerer, Beate M., Drosten, Christian, Brites, Carlos, de Lamballerie, Xavier, Niedrig, Matthias, Netto, Eduardo Martins, and Drexler, Jan Felix
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Medical tests -- Health aspects -- Usage ,Patient care -- Analysis ,Zika virus -- Research -- Health aspects -- Causes of ,Health ,World Health Organization - Abstract
The catastrophic Zika virus outbreak in the Americas has affected millions of persons. Brazil was the most affected country and reported [approximately equal to] 95% of all cases of suspected [...]
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- 2018
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18. Mild COVID-19 despite autoantibodies against type I IFNs in autoimmune polyendocrine syndrome type 1
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Meisel, Christian, Akbil, Bengisu, Meyer, Tim, Lankes, Erwin, Corman, Victor M., Staudacher, Olga, Unterwalder, Nadine, Kolsch, Uwe, Drosten, Christian, Mall, Marcus A., Kallinich, Tilmann, Schnabel, Dirk, Goffinet, Christine, and von Bernuth, Horst
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Interferon -- Health aspects ,Autoantibodies -- Health aspects ,Autoimmune diseases -- Development and progression -- Genetic aspects ,Endocrine gland diseases -- Development and progression -- Genetic aspects ,Health care industry - Abstract
Autoantibodies against IFN-[alpha] and IFN-[omega] (type I IFNs) were recently reported as causative for severe COVID-19 in the general population. Autoantibodies against IFN-[alpha] and IFN-[omega] are present in almost all patients with autoimmune polyendocrine syndrome type 1 (APS-1) caused by biallelic deleterious or heterozygous dominant mutations in AIRE. We therefore hypothesized that autoantibodies against type I IFNs also predispose patients with APS-1 to severe COVID-19. We prospectively studied 6 patients with APS-1 between April 1, 2020 and April 1, 2021. Biobanked pre-COVID-19 sera of APS-1 subjects were tested for neutralizing autoantibodies against IFN-[alpha] and IFN-[omega]. The ability of the patients' sera to block recombinant human IFN-[alpha] and IFN-[omega] was assessed by assays quantifying phosphorylation of signal transducer and activator of transcription 1 (STAT1) as well as infection-based IFN-neutralization assays. We describe 4 patients with APS-1 and preexisting high titers of neutralizing autoantibodies against IFN-[alpha] and IFN-[omega] who contracted SARS-CoV-2, yet developed only mild symptoms of COVID-19. None of the patients developed dyspnea, oxygen requirement, or high temperature. All infected patients with APS-1 were females andyoungerthan 26 years of age. Clinical penetrance of neutralizing autoantibodies against type I IFNs for severe COVID-19 is not complete., Introduction Mutations in AIRE (gene encoding the protein autoimmune regulator) cause autoimmune polyendocrine syndrome type 1 (APS-1) (1-3). AIRE is expressed in thymic epithelium and secondary lymphoid organs (4). AIRE [...]
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- 2021
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19. Lineage-Specific Real-Time Reverse Transcription PCR for Yellow Fever Virus Outbreak Surveillance, Brazil
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Fischer, Carlo, Torres, Maria C., Patel, Pranav, Moreira-Soto, Andres, Gould, Ernest A., Charrel, Remi N., de Lamballerie, Xavier, Nogueira, Rita Maria Ribeiro, Sequeira, Patricia C., Rodrigues, Cintia D.S., Kummerer, Beate M., Drosten, Christian, Landt, Olfert, de Filippis, Ana Maria Bispo, and Drexler, Jan Felix
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Polymerase chain reaction ,Yellow fever -- Development and progression ,Transcription (Genetics) ,Vaccination ,Health - Abstract
Yellow fever virus (YFV) is a mosquitoborne member of the genus Flavivirus within the family Flaviviridae (online Technical Appendix Figure 1, panel A, https://wwwnc.cdc.gov/EID/article/23/11/17-1131-Techapp1.pdf) that is endemic to Africa and [...]
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- 2017
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20. Hepatitis E Virus Infection in European Brown Hares, Germany, 2007-2014
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Corman, Victor Max, Hilgensloh, Laura, Voigt, Ulrich, Marklewitz, Marco, Siebert, Ursula, Drosten, Christian, and Drexler, Jan Felix
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Hepatitis E -- Health aspects ,Medical research -- Health aspects ,Infection -- Health aspects ,Zoonoses -- Health aspects ,Rabbits -- Health aspects ,Genomics -- Health aspects ,Pets ,Climate ,Health - Abstract
Hepatitis E virus (HEV; family Hepeviridae, genus Orthohepevirus) is a major cause of acute hepatitis in humans worldwide. Large outbreaks have included waterborne HEV in the tropics and zoonotic infections [...]
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- 2019
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21. Assay optimization for molecular detection of Zika virus/ Optimisation des tests pour la detection moleculaire du virus Zika/ Optimizacion de la prueba para la deteccion molecular del virus de Zika
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Corman, Victor M., Rasche, Andrea, Baronti, Cecile, Aldabbagh, Souhaib, Cadar, Daniel, Reusken, Chantal BEM, Pas, Suzan D., Goorhuis, Abraham, Schinkel, Janke, Molenkamp, Richard, Kiimmerer, Beate M., Bleicker, Tobias, Brunink, Sebastian, Eschbach-Bludau, Monika, Eis-Hubinger, Anna M., Koopmans, Marion P., Schmidt-Chanasit, Jonas, Grobusch, Martin P., de Lamballerie, Xavier, Drosten, Christian, and Drexler, Jan Felix
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Biological assay -- Innovations ,Genotype -- Identification and classification ,Molecular diagnostic techniques -- Innovations ,Health - Abstract
Objective To examine the diagnostic performance of real-time reverse transcription (RT)-polymerase chain reaction (PCR) assays for Zika virus detection. Methods We compared seven published real-time RT-PCR assays and two new assays that we have developed. To determine the analytical sensitivity of each assay, we constructed a synthetic universal control ribonucleic acid (uncRNA) containing all of the assays' target regions on one RNA strand and spiked human blood or urine with known quantities of African or Asian Zika virus strains. Viral loads in 33 samples from Zika virus-infected patients were determined by using one of the new assays. Findings Oligonucleotides of the published real-time RT-PCR assays, showed up to 10 potential mismatches with the Asian lineage causing the current outbreak, compared with 0 to 4 mismatches for the new assays. The 95% lower detection limit of the seven most sensitive assays ranged from 2.1 to 12.1 uncRNA copies/reaction. Two assays had lower sensitivities of 17.0 and 1373.3 uncRNA copies/reaction and showed a similar sensitivity when using spiked samples. The mean viral loads in samples from Zika virus-infected patients were 5 x 104 RNA copies/mL of blood and 2 x [10.sup.4] RNA coples/mL of urine. Conclusion We provide reagents and updated protocols for Zika virus detection suitable for the current outbreak strains. Some published assays might be unsuitable for Zika virus detection, due to the limited sensitivity and potential incompatibility with some strains. Viral concentrations in the clinical samples were close to the technical detection limit, suggesting that the use of insensitive assays will cause false-negative results. Objectif Etudier la performance diagnostique des tests bases sur l'amplification en chaine par polymerase (PCR) en temps reel apres transcription inverse (RT) pour detecter le virus Zika. Methodes Nous avons compare sept tests publies utilisant la RT-PCR en temps reel et deux nouveaux tests developpes par nos soins. Afin de determiner la sensibilite analytique de chaque test, nous avons concu un acide ribonucleique synthetique de controle universel (ARNcun) contenant toutes les regions ciblees par les tests sur une helice ARN et enrichi de l'urine ou du sang humain de quantites connues de souches africaines ou asiatiques du virus Zika. Les charges virales dans 33 echantillons provenant de patients infectes par le virus Zika ont ete determinees a l'aide de l'un des nouveaux tests. Resultats Les oligonucleotides des tests publies utilisant la RT-PCR en temps reel ont presente jusqu'a TOmauvais apparlements potentiels avec la lignee asiatique provoquant l'epidemie actuelle, alors qu'on a constate de 0 a 4 mauvais appariements dans le cas des nouveaux tests. La limite de detection inferieure a 95% des sept tests les plus sensibles variait de 2,1 a 12,1 copies dARNcun/reaction. Deux tests presentaient des sensibilites plus basses de 17,0 et 1373,3 copies d'ARNcun/reaction et montraient une sensibilite similaire lorsque des echantillons enrichis etaient utilises. Les charges virales moyennes dans les echantillons provenant de patients infectes par le virus Zika etaient de 5 x 104 copies d'ARN/mL de sang et de 2 x 104 copies dARN/mL d'urine. Conclusion Nous proposons pour detecter le virus Zika des reactifs et des protocoles actualises, adaptes aux souches responsables de la flambee actuelle.Tous les tests publies ne permettent pas de detecter le virus Zika en raison d'une sensibilite limitee et d'une incompatibilite potentielle avec certaines souches. Les concentrations virales dans les echantillons cliniques etaient proches de la limite de detection technique, ce qui laisse penser que l'utilisation de tests insensibles donnera des resultats faussement negatifs. Objetivo Examinar el rendimiento del diagnostico de las pruebas de reaccion en cadena de la polimerasa de transcriptasa inversa (RT-PCR) en tiempo real para la deteccion del virus de Zika. Metodos Se compararon siete pruebas de RT-PCR en tiempo real publicadas y dos pruebas nuevas que se han desarrollado. Con el fin de determinar la sensibilidad analitica de cada prueba, se construyo un acido ribonucleico sintetico de control universal (uncRNA) que contenia todas las regiones objetivo de las pruebas en una hebra de RNA y se anadio a sangre u orina humana con cantidades conocidas de cepas de virus de Zika de Asia o Africa. Se determinaron las cargas viricas en 33 muestras procedentes de pacientes infectados por el virus de Zika a traves de una de las pruebas nuevas. Resultados Los oligonucleotidos de las pruebas de RT-PCR en tiempo real publicadas mostraron hasta 10 posibles discordancias con el linaje de Asia causante de los brotes actuales, en comparacion con 0 de 4 discordancias en el caso de las pruebas nuevas. El limite de deteccion inferior del 95% de las siete pruebas mas sensibles abarco de 2,1 a 12,1 copias/reaccion de uncRNA. Dos pruebas mostraron sensibilidades inferiores de 17,0 y 1373,3 copias/reaccion de uncRNA y presentaron una sensibilidad similar al usar muestras Infectadas. Las cargas viricas medias en las muestras procedentes de pacientes infectados por el virus de Zika fueron de 5 x 104 copias de RNA/mL de sangre y de 2 x [10.sup.4] coplas de RNA/ml de orina. Conclusion Se proporcionan reactivos y protocolos actualizados para la deteccion adecuada del virus de Zika en el caso de las cepas de brotes actuales. Algunas pruebas publicadas pueden no ser adecuadas para la deteccion del virus de Zika, debido a la limitada sensibilidad y a la posible incompatibilidad con algunas cepas. Las concentraciones viricas en las muestras clinicas se acercaron al limite de deteccion tecnico, lo que sugeria que el uso de pruebas Intensivas causaria resultados falsos negativos., Introduction The Zika virus is a mosquito-borne flavivirus with an approximately 11 kilobase ribonucleic acid (RNA) genome. (1) The virus usually causes a mild infection in adults, symptoms include fever, [...]
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- 2016
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22. Hepatitis E virus infection in dromedaries, North and East Africa, United Arab Emirates, and Pakistan, 1983-2015
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Rasche, Andrea, Saqib, Muhammad, Liljander, Anne M., Bornstein, Set, Zohaib, Ali, Renneker, Stefanie, Steinhagen, Katja, Wernery, Renate, Younan, Mario, Gluecks, Ilona, Hilali, Mosaad, Musa, Bakri E., Jores, Joerg, Wernery, Ulrich, Drexer, Jan Felix, Drosten, Christian, and Corman, Victor Max
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Hepatitis E -- Health aspects ,Infection -- Health aspects ,Food contamination -- Health aspects ,Health - Abstract
Hepatitis E virus (HEV) is a major cause of acute hepatitis worldwide (1). Four HEV genotypes belonging to the species Orthohepevirus A are commonly found in humans (HEV-1 through HEV-4). [...]
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- 2016
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23. Mers-cov antibodies in humans, Africa, 2013-2014
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Liljander, Anne, Meyer, Benjamin, Jores, Joerg, Muller, Marcel A., Lattwein, Erik, Njeru, Ian, Bett, Bernard, Drosten, Christian, and Corman, Victor Max
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Viral antibodies -- Health aspects ,Antibodies -- Health aspects ,Livestock -- Health aspects ,Health - Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) infection causes severe respiratory illness in humans. Some cases have been sporadic, but others have been part of nosocomial outbreaks mainly on the Arabian [...]
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- 2016
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24. Microevolution of outbreak-associated Middle East respiratory syndrome coronavirus, South Korea, 2015
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Seong, Moon-Woo, Kim, So Yeon, Corman, Victor Max, Kim, Taek Soo, Cho, Sung Im, Kim, Man Jin, Lee, Seung Jun, Lee, Jee-Soo, Seo, Soo Hyun, Ahn, Ji Soo, Yu, Byeong Su, Park, Nare, Oh, Myoung-don, Park, Wan Beom, Lee, Ji Yeon, Kim, Gayeon, Joh, Joon Sung, Jeong, Ina, Kim, Eui Chong, Drosten, Christian, and Park, Sung Sup
- Subjects
Evolution -- Analysis ,Genomics -- Analysis ,Phylogeny -- Analysis ,Health - Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV), first detected in Saudi Arabia in 2012, is a novel human pathogen that causes severe respiratory illness (1). Phylogenetic analyses and transmission studies suggest [...]
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- 2016
- Full Text
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25. No evidence of Gouleako and Herbert virus infections in pigs, cote d'Ivoire and Ghana
- Author
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Junglen, Sandra, Marklewitz, Marco, Zirkel, Florian, Wollny, Robert, Meyer, Benjamin, Heidemann, Hanna, Metzger, Sonja, Annan, Augustina, Dei, Dickson, Leendertz, Fabian H., Oppong, Samuel, and Drosten, Christian
- Subjects
Antiviral agents -- Analysis ,Swine -- Analysis ,Biological response modifiers -- Analysis ,Infection -- Analysis ,Resveratrol -- Analysis ,Health - Abstract
Orthobunyaviruses and phleboviruses are transmitted to animals and humans by blood-feeding arthropods such as mosquitoes, sandflies, and ticks (1,2). Infection can cause systemic disease, including encephalitis or hemorrhagic fevers. Members [...]
- Published
- 2015
- Full Text
- View/download PDF
26. Occupational exposure to dromedaries and risk for MERS-CoV infection, Qatar, 2013-2014
- Author
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Reusken, Chantal B.E.M., Farag, Elmoubasher A.B.A., Haagmans, Bart L., Mohran, Khaled A., Godeke, Gert-Jan, Raj, V. Stalin, Alhajri, Farhoud, Marri, Salih A. Al-, Romaihi, Hamad E. Al-, Thani, Mohamed Al-, Bosch, Berend-Jan, van der Eijk, Annemiek A., Sayed, Ahmed M. El-, Ibrahim, Adel K., Molawi, N. Al-, Muller, Marcel A., Pasha, Syed K., Drosten, Christian, AlHajri, Mohd M., and Koopmans, Marion P.G.
- Subjects
Resveratrol ,Health - Abstract
Since Middle East respiratory syndrome coronavirus (MERS-CoV) was first detected in 2012, approximately 1,000 human infections have been reported to the World Health Organization, all linked to residence in or [...]
- Published
- 2015
- Full Text
- View/download PDF
27. Human-bat interactions in rural West Africa
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Anti, Priscilla, Owusu, Michael, Agbenyega, Olivia, Annan, Augustina, Badu, Ebenezer Kofi, Nkrumah, Evans Ewald, Tschapka, Marco, Oppong, Samuel, Adu-Sarkodie, Yaw, and Drosten, Christian
- Subjects
Bats ,Disease transmission ,Human-animal relationships ,Health - Abstract
Bats are increasingly being recognized as hosts for pathogens that affect humans and livestock (1). The 2014-2015 outbreak of Ebola virus disease in West Africa demonstrates how human-bat interactions in [...]
- Published
- 2015
- Full Text
- View/download PDF
28. Novel arenavirus isolates from Namaqua rock mice, Namibia, southern Africa
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Witkowski, Peter T., Kallies, Rene, Hoveka, Julia, Auste, Brita, Ithete, Ndapewa L., Soltys, Katarina, Szemes, Tomas, Drosten, Christian, Preiser, Wolfgang, Klempa, Boris, Mfune, John K.E., and Kruger, Detlev H.
- Subjects
Health - Abstract
Arenaviruses are known to cause severe hemorrhagic fevers across the globe with case fatality rates up to 30% (1). The viruses possess a bisegmented, single-stranded RNA genome with ambisense coding [...]
- Published
- 2015
- Full Text
- View/download PDF
29. Acute Middle East respiratory syndrome coronavirus infection in livestock dromedaries, Dubai, 2014
- Author
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Wernery, Ulrich, Corman, Victor M., Wong, Emily Y.M., Tsang, Alan K.L., Muth, Doreen, Lau, Susanna K.P., Khazanehdari, Kamal, Zirkel, Florian, Ali, Mansoor, Nagy, Peter, Juhasz, Jutka, Wernery, Renate, Joseph, Sunitha, Syriac, Ginu, Elizabeth, Shyna K., Patteril, Nissy Annie Georgy, Woo, Patrick C.Y., and Drosten, Christian
- Subjects
Coronaviruses -- Identification and classification -- Health aspects ,Livestock -- Diseases -- Medical examination ,Company distribution practices ,Health - Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) causes outbreaks and isolated cases of severe respiratory disease in humans. The virus is transmissible from human to human, but the focus of infection [...]
- Published
- 2015
- Full Text
- View/download PDF
30. Time course of MERS-CoV infection and immunity in dromedary camels
- Author
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Meyer, Benjamin, Juhasz, Judit, Barua, Rajib, Gupta, Aungshuman Das, Hakimuddin, Fatima, Corman, Victor M., Muller, Marcel A., Wernery, Ulrich, Drosten, Christian, and Nagy, Peter
- Subjects
Vaccination -- Dosage and administration ,Viral antibodies -- Research ,Antibodies -- Research ,Health - Abstract
In 2012, Middle East respiratory syndrome coronavirus (MERS-CoV) emerged on the Arabian Peninsula (1). As of February 2016 the virus has caused 1,638 human infections, including 587 deaths (2). Zoonotic [...]
- Published
- 2016
- Full Text
- View/download PDF
31. Antibodies against MERS Coronavirus in Dromedary Camels, Kenya, 1992-2013
- Author
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Corman, Victor M., Jores, Joerg, Meyer, Benjamin, Younan, Mario, Liljander, Anne, Said, Mohammed Y., Gluecks, Ilona, Lattwein, Erik, Bosch, Berend-Jan, Drexler, Jan Felix, Bornstein, Set, Drosten, Christian, and Muller, Marcel A.
- Subjects
Camels -- Health aspects ,Viral antibodies -- Research -- Usage -- Health aspects ,Antibodies -- Research -- Usage -- Health aspects ,Health - Abstract
Middle East respiratory syndrome coronavirus (MERSCoV) was discovered in a patient from Saudi Arabia in 2012 and has since caused ≥ 250 human infections and 93 deaths (1). The evolutionary [...]
- Published
- 2014
- Full Text
- View/download PDF
32. Human infection with MERS Coronavirus after exposure to infected camels, Saudi Arabia, 2013
- Author
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Memish, Ziad A., Cotten, Matthew, Meyer, Benjamin, Watson, Simon J., Alsahafi, Abdullah J., Rabeeah, Abdullah A. Al, Corman, Victor Max, Sieberg, Andrea, Makhdoom, Hatem Q., Assiri, Abdullah, Masri, Malaki Al, Aldabbagh, Souhaib, Bosch, Berend-Jan, Beer, Martin, Muller, Marcel A., Kellam, Paul, and Drosten, Christian
- Subjects
Camels -- Influence ,Disease transmission -- Research ,Coronaviruses -- Influence ,Health - Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) was identified in 2012 in a cell culture taken from a patient who died of pneumonia in Saudi Arabia (1). Since 2012, at least [...]
- Published
- 2014
- Full Text
- View/download PDF
33. Contact investigation for imported case of middle East respiratory syndrome, Germany
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Reuss, Annicka, Litterst, Annette, Drosten, Christian, Seilmaier, Michael, Bohmer, Merle, Graf, Petra, Gold, Hermann, Wendtner, Clemens-Martin, Zanuzdana, Arina, Schaade, Lars, Haas, Walter, and Buchholz, Udo
- Subjects
Infection control ,Cross infection ,Nosocomial infections ,Company legal issue ,Health ,World Health Organization -- Investigations - Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) infection was initially reported to the World Health Organization (WHO) in September 2012 (1,2). By November 11, 2013, a total of 153 laboratory-confirmed cases [...]
- Published
- 2014
- Full Text
- View/download PDF
34. Antibodies against MERS Coronavirus in dromedaries, United Arab Emirates, 2003 and 2013
- Author
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Meyer, Benjamin, Muller, Marcel A., Corman, Victor M., Reusken, Chantal B.E.M., Ritz, Daniel, Godeke, Gert-Jan, Lattwein, Erik, Kallies, Stephan, Siemens, Artem, van Beek, Janko, Drexler, Jan F., Muth, Doreen, Bosch, Berend-Jan, Wernery, Ulrich, Koopmans, Marion P.G., Wernery, Renate, and Drosten, Christian
- Subjects
Viral antibodies -- Health aspects -- Analysis ,Antibodies -- Health aspects -- Analysis ,Health - Abstract
Middle East respiratory syndrome coronavirus (MERSCoV) is an emerging pathogen associated with severe respiratory symptoms and renal failure in infected patients (1,2). Globally, 156 laboratory-confirmed cases of infection with MERS-CoV, [...]
- Published
- 2014
- Full Text
- View/download PDF
35. Replicative capacity of MERS coronavirus in livestock cell lines
- Author
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Eckerle, Isabella, Corman, Victor M., Muller, Marcel A., Lenk, Matthias, Ulrich, Rainer G., and Drosten, Christian
- Subjects
Severe acute respiratory syndrome -- Health aspects ,Livestock -- Health aspects ,Health - Abstract
Coronaviruses (CoV) in the genera Alphacoronavirus and Betacoronavirus (order Nidovirales, family Coronaviridae, subfamily Coronavirinae) infect a broad range of mammals, including humans (1). The human CoVs (HCoVs) HCoV-HKU1, HCoV-229E, HCoV-NL63, [...]
- Published
- 2014
- Full Text
- View/download PDF
36. Early introduction and delayed dissemination of pandemic influenza, Gabon
- Author
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Lekana-Douki, Sonia Etenna, Mouinga-Ondeme, Augustin, Nkoghe, Dieudonne, Drosten, Christian, Drexler, Jan Felix, Kazanji, Mirdad, and Leroy, Eric M.
- Subjects
Epidemics -- Gabon -- Health aspects ,Influenza -- Health aspects ,Public health -- Health aspects ,Health - Abstract
In April 2009, a pandemic strain of influenza A (H1N1) (pH1N1) virus emerged in Mexico and the United States; the World Health Organization declared a pandemic alert on June 11, [...]
- Published
- 2013
- Full Text
- View/download PDF
37. Dipeptidyl peptidase 4 is a functional receptor for the emerging human coronavirus-EMC
- Author
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Raj, V. Stalin, Mou, Huihui, Smits, Saskia L., Dekkers, Dick H.W., Muller, Marcel A., Dijkman, Ronald, Muth, Doreen, Demmers, Jeroen A.A., Zaki, Ali, Fouchier, Ron A.M., Thiel, Volker, Drosten, Christian, Rottier, Peter J.M., Osterhaus, Albert D.M.E., Bosch, Berend Jan, and Haagmans, Bart L.
- Subjects
Coronavirus infections -- Diagnosis -- Complications and side effects -- Causes of ,Medical research ,Medicine, Experimental ,Ubiquitin-proteasome system -- Research ,Proteases -- Physiological aspects ,Coronaviruses -- Physiological aspects ,Environmental issues ,Science and technology ,Zoology and wildlife conservation - Abstract
Most human coronaviruses cause mild upper respiratory tract disease but may be associated with more severe pulmonary disease in immunocompromised individuals (1). However, SARS coronavirus caused severe lower respiratory disease with nearly 10% mortality and evidence of systemic spread (2). Recently, another coronavirus (human coronavirus-Erasmus Medical Center (hCoV-EMC)) was identified in patients with severe and sometimes lethal lower respiratory tract infection (3'4). Viral genome analysis revealed close relatedness to coronaviruses found in bats (5). Here we identify dipeptidyl peptidase 4 (DPP4; also known as CD26) as a functional receptor for hCoV-EMC. DPP4 specifically co-purified with the receptor-binding S1 domain of the hCoV-EMC spike protein from lysates of susceptible Huh-7 cells. Antibodies directed against DPP4 inhibited hCoV-EMC infection of primary human bronchial epithelial cells and Huh-7 cells. Expression of human and bat (Pipistrellus pipistrellus) DPP4 in non-susceptible COS-7 cells enabled infection by hCoV-EMC. The use of the evolutionarily conserved DPP4 protein from different species as a functional receptor provides clues about the host range potential of hCoV-EMC. In addition, it will contribute critically to our understanding of the pathogenesis and epidemiology of this emerging human coronavirus, and may facilitate the development of intervention strategies., Coronaviruses infect a wide range of mammals and birds. Their tropism is primarily determined by the ability of the spike (S) entry protein to bind to a cell surface receptor. [...]
- Published
- 2013
38. Human betacoronavirus 2c EMC/2012-related viruses in bats, Ghana and Europe
- Author
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Annan, Augustina, Baldwin, Heather J., Corman, Victor Max, Klose, Stefan M., Owusu, Michael, Nkrumah, Evans Ewald, Badu, Ebenezer Kofi, Anti, Priscilla, Agbenyega, Olivia, Meyer, Benjamin, Oppong, Samuel, Sarkodie, Yaw Adu, Kalko, Elisabeth K.V., Lina, Peter H.C., Godlevska, Elena V., Reusken, Chantal, Seebens, Antje, Gloza-Rausch, Florian, Vallo, Peter, Tschapka, Marco, Drosten, Christian, and Drexler, Jan Felix
- Subjects
Bats -- Diseases and pests -- Research ,Coronaviruses -- Physiological aspects -- Distribution -- Research ,Company distribution practices ,Health - Abstract
Coronaviruses (CoVs) are enveloped viruses with a positive-sense, single-stranded RNA genome (1). CoVs are classified into 4 genera: Alphacoronavirus, Betacoronavirus (grouped further into clades 2a-2d), Gammacoronavirus, and Deltacoronavirus. Two human [...]
- Published
- 2013
- Full Text
- View/download PDF
39. Roadblocks to translational challenges on viral pathogenesis
- Author
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Deeks, Steven, Drosten, Christian, Picker, Louis, Subbarao, Kanta, and Suzich, JoAnn
- Subjects
Virus research -- Analysis -- Planning -- Management ,Company business planning ,Company business management ,Biological sciences ,Health - Abstract
Distinct roadblocks prevent translating basic findings in viral pathogenesis into therapies and implementing potential solutions in the clinic. An ongoing partnership between the Volkswagen Foundation and Nature Medicine resulted in an interactive meeting in 2012, as part of the 'Herrenhausen Symposia' series. Current challenges for various fields of viral research were recognized and discussed with a goal in mind--to identify solutions and propose an agenda to address the translational barriers. Here, some of the researchers who participated at the meeting provide a concise outlook at the most pressing unmet research and clinical needs, identifying these key obstacles is a necessary step towards the prevention and cure of human viral diseases., Moving ahead on HIV prevention and cure One of the most dramatic therapeutic advances in the history of medicine was the development and clinical implementation of antiretroviral therapy (ART) for [...]
- Published
- 2013
40. Human parvovirus 4 in nasal and fecal specimens from children, Ghana
- Author
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Drexler, Jan Felix, Reber, Ulrike, Muth, Doreen, Herzog, Petra, Annan, Augustina, Ebach, Fabian, Sarpong, Nimarko, Acquah, Samuel, Adlkofer, Julia, Adu-Sarkodie, Yaw, Panning, Marcus, Tannich, Egbert, May, Jurgen, Drosten, Christian, and Eis-Hubinger, Anna Maria
- Subjects
Parvovirus infections -- Risk factors -- Research -- Diagnosis ,Children -- Health aspects ,Disease transmission -- Research ,Health - Abstract
Nonparenteral transmission might contribute to human parvovirus 4 (PARV4) infections in sub-Saharan Africa. PARV4 DNA was detected in 8 (0.83%) of 961 nasal samples and 5 (0.53%) of 943 fecal [...]
- Published
- 2012
- Full Text
- View/download PDF
41. Cosavirus infection in persons with and without gastroenteritis, Brazil
- Author
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Stocker, Andreas, Souza, Breno Frederico de Carvalho Dominguez, Ribeiro, Tereza Cristina Medrado, Netto, Eduardo Martins, Araujo, Luciana Oliveira, Correa, Jefferson Ivan, Almeida, Patricia Silva, de Mattos, Angela Peixoto, Ribeiro, Hugo da Costa, Jr., Pedral-Sampaio, Diana Brasil, Drosten, Christian, and Drexler, Jan Felix
- Subjects
Medical research -- Health aspects ,Medicine, Experimental -- Health aspects ,Gastroenteritis -- Health aspects ,RNA -- Health aspects ,HIV patients -- Health aspects ,Health - Abstract
The family Picornaviridae comprises 12 genera and includes several leading pathogens affecting human and animal health, e.g., foot-and-mouth-disease virus and polioviruses. With the advent of metagenomics, 3 novel human picornaviruses [...]
- Published
- 2012
- Full Text
- View/download PDF
42. Aichi virus shedding in high concentrations in patients with acute diarrhea
- Author
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Drexler, Jan Felix, Baumgarte, Sigrid, de Souza Luna, Luciano Kleber, Eschbach-Bludau, Monika, Lukashev, Alexander N., and Drosten, Christian
- Subjects
Gastroenteritis -- Care and treatment -- Health aspects ,Diarrhea -- Care and treatment -- Health aspects ,Prevalence studies (Epidemiology) -- Health aspects ,RNA -- Health aspects ,Health - Abstract
The family Picornaviridae includes 12 established genera, and representatives of 5 of these have been found in humans (Enterovirus, Hepatovirus, Parechovirus, Cardiovirus, and Kobuvirus). Among those, human pathogenicity has been [...]
- Published
- 2011
- Full Text
- View/download PDF
43. Amplification of emerging viruses in a bat colony
- Author
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Drexler, Jan Felix, Corman, Victor Max, Wegner, Tom, Tateno, Adriana Fumie, Zerbinati, Rodrigo Melim, Gloza-Rausch, Florian, Seebens, Antje, Muller, Marcel A., and Drosten, Christian
- Subjects
Bats -- Diseases and pests -- Genetic aspects -- Control ,Viruses -- Identification and classification -- Genetic aspects -- Control ,Health - Abstract
Bats (Chiroptera) constitute ≅ 20% of living mammal species and are distributed on all continents except Antarctica (1). Their ability to fly and migrate, as well as the large sizes [...]
- Published
- 2011
- Full Text
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44. Dengue virus serotype 3 infection in traveler returning from West Africa to Germany
- Author
-
Eckerle, Isabella, Kapaun, Annette, Junghanss, Thomas, Schnitzler, Paul, Drosten, Christian, and Janisch, Thomas
- Subjects
Medical research ,Medicine, Experimental ,Medical geography -- Research ,Dengue -- Case studies -- Diagnosis -- Care and treatment ,Health - Abstract
To the Editor: Dengue virus (DENV) is a member of the family Flaviviridae, genus Flavivirus, and comprises 4 serotypes (DENV-1, DENV-2, DENV-3, and DENV-4). DENV is transmitted by Aedes spp. [...]
- Published
- 2015
45. Distant relatives of severe acute respiratory syndrome coronavirus and close relatives of human coronavirus 229E in bats, Ghana
- Author
-
Pfefferle, Susanne, Oppong, Samuel, Drexler, Jan Felix, Gloza-Rausch, Florian, Ipsen, Anne, Seebens, Antje, Muller, Marcel A., Annan, Augustina, Vallo, Peter, Adu-Sarkodie, Yaw, Kruppa, Thomas F., and Drosten, Christian
- Subjects
RNA -- Research ,RNA -- Physiological aspects ,Severe acute respiratory syndrome -- Causes of ,Severe acute respiratory syndrome -- Research ,Coronaviruses -- Health aspects ,Coronaviruses -- Research - Abstract
Coronaviruses (CoVs) (order Nidovirales, family Coronaviridae, genus Coronavirus) are enveloped viruses with plus-stranded RNA genomes of 26-32 kb, the largest contiguous RNA genomes in nature (1). They are classified into [...]
- Published
- 2009
- Full Text
- View/download PDF
46. A novel diagnostic target in the Hepatitis C virus genome
- Author
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Drexler, Jan Felix, Kupfer, Bernd, Petersen, Nadine, Grotto, Rejane Maria Tommasini, Rodrigues, Silvia Maria Corvino, Grywna, Klaus, Panning, Marcus, Annan, Augustina, Silva, Giovanni Faria, Douglas, Jill, Koay, Evelyn S.C., Smuts, Heidi, Netto, Eduardo M., Simmonds, Peter, de Moura Campos Pardini, Maria Ines, Roth, W. Kurt, and Drosten, Christian
- Subjects
Hepatitis C -- Diagnosis ,Hepatitis C -- Genetic aspects ,Hepatitis C -- Research ,Hepatitis C virus -- Genetic aspects ,Hepatitis C virus -- Research ,Nucleotide sequencing -- Usage ,Nucleotide sequencing -- Research ,Viremia -- Measurement ,Viremia -- Usage ,Viremia -- Research - Abstract
Background Detection and quantification of hepatitis C virus (HCV) RNA is integral to diagnostic and therapeutic regimens. All molecular assays target the viral 5'-noncoding region (5'-NCR), and all show genotype-dependent variation of sensitivities and viral load results. Non-western HCV genotypes have been under-represented in evaluation studies. An alternative diagnostic target region within the HCV genome could facilitate a new generation of assays. Methods and Findings In this study we determined by de novo sequencing that the 3'-X-tail element, characterized significantly later than the rest of the genome, is highly conserved across genotypes. To prove its clinical utility as a molecular diagnostic target, a prototype qualitative and quantitative test was developed and evaluated multicentrically on a large and complete panel of 725 clinical plasma samples, covering HCV genotypes 1-6, from four continents (Germany, UK, Brazil, South Africa, Singapore). To our knowledge, this is the most diversified and comprehensive panel of clinical and genotype specimens used in HCV nucleic acid testing (NAT) validation to date. The lower limit of detection (LOD) was 18.4 IU/ml (95% confidence interval, 15.3-24.1 IU/ml), suggesting applicability in donor blood screening. The upper LOD exceeded [10.sup.-9] IU/ml, facilitating viral load monitoring within a wide dynamic range. In 598 genotyped samples, quantified by Bayer VERSANT 3.0 branched DNA (bDNA), X-tail-based viral loads were highly concordant with bDNA for all genotypes. Correlation coefficients between bDNA and X-tail NAT, for genotypes 1-6, were: 0.92, 0.85, 0.95, 0.91, 0.95, and 0.96, respectively; X-tail-based viral loads deviated by more than 0.5 log10 from 5'-NCR-based viral loads in only 12% of samples (maximum deviation, 0.85 log10). The successful introduction of X-tail NAT in a Brazilian laboratory confirmed the practical stability and robustness of the X-tail-based protocol. The assay was implemented at low reaction costs (US$8.70 per sample), short turnover times (2.5 h for up to 96 samples), and without technical difficulties. Conclusion This study indicates a way to fundamentally improve HCV viral load monitoring and infection screening. Our prototype assay can serve as a template for a new generation of viral load assays. Additionally, to our knowledge this study provides the first open protocol to permit industry-grade HCV detection and quantification in resource-limited settings., Introduction Hepatitis C virus (HCV) is one of the leading causes of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma [1,2]. Seroprevalence studies suggest that at least 170 million individuals have [...]
- Published
- 2009
47. Novel human parechovirus from Brazil
- Author
-
Drexler, Jan Felix, Grywna, Klaus, Stocker, Andreas, Almeida, Patricia Silva, Ribeiro, Tereza Cristina Medrado, Eschbach-Bludau, Monika, Petersen, Nadine, da Costa Ribeiro, Hugo, Jr., and Drosten, Christian
- Subjects
Company distribution practices ,Disease transmission -- Research ,Picornavirus infections -- Causes of ,Picornavirus infections -- Diagnosis ,Picornavirus infections -- Distribution ,Picornavirus infections -- Control ,Prevalence studies (Epidemiology) - Abstract
Human parechoviruses (HPeVs) were detected by reverse transcription-PCR in 16.1% of 335 stool samples from children ********** The human parechovirus (HPeV) species are small, nonenveloped RNA viruses that belong to [...]
- Published
- 2009
48. MERS coronavirus neutralizing antibodies in camels, Eastern Africa, 1983-1997
- Author
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Muller, Marcel A., Corman, Victor Max, Jores, Joerg, Meyer, Benjamin, Younan, Mario, Liljander, Anne, Bosch, Berend-Jan, Lattwein, Erik, Hilali, Mosaad, Musa, Bakri E., Bornstein, Set, and Drosten, Christian
- Subjects
Viral antibodies -- Health aspects ,Antibodies -- Health aspects ,Health - Abstract
Since 2012, a newly emerged human pathogenic coronavirus (CoV) has caused an ongoing epidemic on the Arabian Peninsula. The designated Middle East respiratory syndrome (MERS)-CoV belongs to the Betacoronavirus genus [...]
- Published
- 2014
- Full Text
- View/download PDF
49. Circulation of 3 lineages of a novel Saffold cardiovirus in humans
- Author
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Drexler, Jan Felix, Luna, Luciano Kleber de Souza, Stocker, Andreas, Almeida, Patricia Silva, Ribeiro, Tereza Cristina Medrado, Petersen, Nadine, Herzog, Petra, Pedroso, Calia, Huppertz, Hans Iko, da Costa Ribeiro, Hugo, Jr., Baumgarte, Sigrid, and Drosten, Christian
- Subjects
Gastroenteritis -- Risk factors ,Gastroenteritis -- Diagnosis ,Gastroenteritis -- Research ,Viruses -- Identification and classification ,Viruses -- Health aspects ,Viruses -- Research ,Genetic screening -- Methods ,Genetic screening -- Health aspects - Abstract
Cardioviruses cause serious disease, mainly in rodents, including diabetes, myocarditis, encephalomyelitis, and multiple sclerosis-like disseminated encephalomyelitis. Recently, a human virus isolate obtained 25 years ago, termed Saffold virus, was sequenced [...]
- Published
- 2008
50. Detection and prevalence patterns of group I coronaviruses in bats, Northern Germany
- Author
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Gloza-Rausch, Florian, Ipsen, Anne, Seebens, Antje, Gottsche, Matthias, Panning, Marcus, Drexler, Jan Felix, Petersen, Nadine, Annan, Augustina, Grywna, Klaus, Muller, Marcel, Pfefferle, Susanne, and Drosten, Christian
- Subjects
Company distribution practices ,Bats -- Genetic aspects ,Bats -- Research ,Coronavirus infections -- Risk factors ,Coronavirus infections -- Distribution ,Coronavirus infections -- Research ,Coronaviruses -- Genetic aspects ,Coronaviruses -- Research - Abstract
We tested 315 bats from 7 different bat species in northern Germany for coronaviruses by reverse transcription-PCR. The overall prevalence was 9.8%. There were 4 lineages of group I coronaviruses [...]
- Published
- 2008
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