9 results on '"Cunningham, Adam F"'
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2. Inflammation drives thrombosis after Salmonella infection via CLEC-2 on platelets
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Hitchcock, Jessica R., Cook, Charlotte N., Bobat, Saeeda, Ross, Ewan A., Flores-Langarica, Adriana, Lowe, Kate L., Khan, Mahmood, Dominguez-Medina, C. Coral, Lax, Sian, Carvalho-Gaspar, Manuela, Hubscher, Stefan, Rainger, G. Ed, Cobbold, Mark, Buckley, Christopher D., Mitchell, Tim J., Mitchell, Andrea, Jones, Nick D., Van Rooijen, N., Kirchhofer, Daniel, Henderson, Ian R., Adams, David H., Watson, Steve P., and Cunningham, Adam F.
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Blood clot -- Development and progression ,Inflammation -- Complications and side effects ,Thrombosis -- Development and progression ,Blood platelets -- Properties ,Cell receptors -- Properties ,Salmonellosis -- Complications and side effects ,Health care industry - Abstract
Thrombosis is a common, life-threatening consequence of systemic infection; however, the underlying mechanisms that drive the formation of infection-associated thrombi are poorly understood. Here, using a mouse model of systemic Salmonella Typhimurium infection, we determined that inflammation in tissues triggers thrombosis within vessels via ligation of C-type lectin-like receptor-2 (CLEC-2) on platelets by podoplanin exposed to the vasculature following breaching of the vessel wall. During infection, mice developed thrombi that persisted for weeks within the liver. Bacteria triggered but did not maintain this process, as thrombosis peaked at times when bacteremia was absent and bacteria in tissues were reduced by more than 90% from their peak levels. Thrombus development was triggered by an innate, TLR4-dependent inflammatory cascade that was independent of classical glycoprotein VI-mediated (GPVI-mediated) platelet activation. After infection, IFN-γ release enhanced the number of podoplanin-expressing monocytes and Kupffer cells in the hepatic parenchyma and perivascular sites and absence of TLR4, IFN-γ, or depletion of monocytic-lineage cells or CLEC-2 on platelets markedly inhibited the process. Together, our data indicate that infection-driven thrombosis follows local inflammation and upregulation of podoplanin and platelet activation. The identification of this pathway offers potential therapeutic opportunities to control the devastating consequences of infection-driven thrombosis without increasing the risk of bleeding., Introduction Thrombosis-associated events are among the leading causes of death worldwide. Systemic infections caused by a plethora of bacterial genera can initiate thrombus development. While the mechanisms that underlie this [...]
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- 2015
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3. Homeostatic regulation of T cell trafficking by a B cell-derived peptide is impaired in autoimmune and chronic inflammatory disease
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Chimen, Myriam, McGettrick, Helen M., Apta, Bonita, Kuravi, Sahithi J., Yates, Clara M., Kennedy, Amy, Odedra, Arjun, Alassiri, Mohammed, Harrison, Matthew, Martin, Ashley, Barone, Francesca, Nayar, Saba, Hitchcock, Jessica R., Cunningham, Adam F., Raza, Karim, Filer, Andrew, Copland, David A., Dick, Andrew D., Robinson, Joseph, Kalia, Neena, Walker, Lucy S.K., Buckley, Christopher D., Nash, Gerard B., Narendran, Parth, and Rainger, G. Ed
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T cells -- Health aspects ,Inflammation ,Homeostasis -- Health aspects ,B cells -- Health aspects ,Cellular control mechanisms -- Health aspects ,Autoimmune diseases -- Physiological aspects ,Peptides -- Health aspects ,Biological sciences ,Health - Abstract
During an inflammatory response, lymphocyte recruitment into tissue must be tightly controlled because dysregulated trafficking contributes to the pathogenesis of chronic disease. Here we show that during inflammation and in response to adiponectin, B cells tonically inhibit T cell trafficking by secreting a peptide (PEPITEM) proteolytically derived from 14.3.3 zeta delta (14.3.3. θδ) protein. PEPITEM binds cadherin-15 on endothelial cells, promoting synthesis and release of sphingosine-1 phosphate, which inhibits trafficking of T cells without affecting recruitment of other leukocytes. Expression of adiponectin receptors on B cells and adiponectin-induced PEPITEM secretion wanes with age, implying immune senescence of the pathway. Additionally, these changes are evident in individuals with type 1 diabetes or rheumatoid arthritis, and circulating PEPITEM in patient serum is reduced compared to that of healthy age-matched donors. In both diseases, tonic inhibition of T cell trafficking across inflamed endothelium is lost. Control of patient T cell trafficking is re-established by treatment with exogenous PEPITEM. Moreover, in animal models of peritonitis, hepatic ischemia-reperfusion injury, Salmonella infection, uveitis and Sjogren's syndrome, PEPITEM reduced T cell recruitment into inflamed tissues., In vertebrates, a lymphocyte (T cell and B cell)-based adaptive immune system has evolved to augment innate immunity. Adaptive responses require lymphocyte trafficking between the bone marrow, lymphoid organs and [...]
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- 2015
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4. A commensal gone bad: complete genome sequence of the prototypical enterotoxigenic Escherichia coli strain H10407
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Crossman, Lisa C., Chaudhuri, Roy R., Beatson, Scott A., Wells, Timothy J., Desvaux, Mickael, Cunningham, Adam F., Petty, Nicola K., Mahon, Vivienne, Brinkley, Carl, Hobman, Jon L., Savarino, Stephen J., Turner, Susan M., Pallen, Mark J., Penn, Charles W., Parkhill, Julian, Turner, A. Keith, Johnson, Timothy J., Thomson, Nicholas R., Smith, Stephen G.J., and Henderson, Ian R.
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Bacterial genetics -- Research ,Escherichia coli -- Genetic aspects ,Genomes -- Identification and classification ,Biological sciences - Abstract
In most cases, Escherichia coli exists as a harmless commensal organism, but it may on occasion cause intestinal and/or extraintestinal disease. Enterotoxigenic E. coli (ETEC) is the predominant cause of E. coli-mediated diarrhea in the developing world and is responsible for a significant portion of pediatric deaths. In this study, we determined the complete genomic sequence of E. coli H10407, a prototypical strain of enterotoxigenic E. coli, which reproducibly elicits diarrhea in human volunteer studies. We performed genomic and phylogenetic comparisons with other E. coli strains, revealing that the chromosome is closely related to that of the nonpathogenic commensal strain E. coli HS and to those of the laboratory strains E. coli K-12 and C. Furthermore, these analyses demonstrated that there were no chromosomally encoded factors unique to any sequenced ETEC strains. Comparison of the E. coli H10407 plasmids with those from several ETEC strains revealed that the plasmids had a mosaic structure but that several loci were conserved among ETEC strains. This study provides a genetic context for the vast amount of experimental and epidemiological data that have been published. doi: 10.1128/JB.00710-10
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- 2010
5. IFN-[gamma] produced by CD8 T cells induces T-bet-dependent and- independent class switching in B cells in responses to alum-precipitated protein vaccine
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Mohr, Elodie, Cunningham, Adam F., Toellner, Kai-Michael, Bobat, Saeeda, Coughlan, Ruth E., Bird, Roger A., MacLennan, Ian C.M., and Serre, Karine
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Vaccines -- Research ,B cells -- Properties ,Immunoglobulin G -- Properties ,T cells -- Properties ,Transcription factors -- Properties ,Science and technology - Abstract
Alum-precipitated protein (alum protein) vaccines elicit long-lasting neutralizing antibody responses that prevent bacterial exotoxins and viruses from entering cells. Typically, these vaccines induce CD4 T cells to become T helper 2 (Th2) cells that induce Ig class switching to IgG1. We now report that CD8 T cells also respond to alum proteins, proliferating extensively and producing IFN-[gamma], a key Th1 cytokine. These findings led us to question whether adoptive transfer of antigen-specific CD8 T cells alters the characteristic CD4 Th2 response to alum proteins and the switching pattern in responding B cells. To this end, WT mice given transgenic ovalbumin (OVA)specific CD4 (OTII) or CD8 (OTI) T cells, or both, were immunized with alum-precipitated OVA. Cotransfer of antigen-specific CD8 T cells skewed switching patterns in responding B cells from IgG1 to IgG2a and IgG2b. Blocking with anti--IFN-[gamma] antibody largely inhibited this altered B-cell switching pattern. The transcription factor T-bet is required in B cells for IFN-[gamma]--dependent switching to IgG2a. By contrast, we show that this transcription factor is dispensable in B cells both for IFN-[gamma]--induced switching to IgG2b and for inhibition of switching to IgG1. Thus, T-bet dependence identifies distinct transcriptional pathways in B cells that regulate IFN-[gamma]--induced switching to different IgG isotypes. B-cell Ig class switch | T helper 1 cells | T helper 2 cells | IgG2a | IgG2b doi/ 10.1073/pnas.1004879107
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- 2010
6. Dysregulated humoral immunity to nontyphoidal Salmonella in HIV-infected African adults
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MacLennan, Carman A., Gilchrist, James J., Gordon, Melita A., Cunningham, Adam F., Cobbold, Mark, Goodall, Margaret, Kingsley, Robert A., van Oosterhout, Joep J.G., Msefula, Chisomo L., Mandala, Wilson L., Leyton, Denisse L., Marshall, Jennifer L., Gondwe, Esther N., Bobat, Saeeda, Lopez-Macias, Constantino, Doffinger, Rainer, Henderson, Ian R., Zijlstra, Eduard E., Dougan, Gordon, Drayson, Mark T., MacLennan, Ian C.M., and Molyneux, Malcolm E.
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Membrane proteins -- Properties ,Mitogens -- Properties ,HIV patients -- Physiological aspects ,Salmonellosis -- Development and progression ,Immune response -- Research ,Science and technology - Abstract
Nontyphoidal Salmonellae are a major cause of life-threatening bacteremia among HIV-infected individuals. Although cell-mediated immunity controls intracellular infection, antibodies protect against Salmonella bacteremia. We report that high-titer antibodies specific for Salmonella lipopolysaccharide (LPS) are associated with a Lack of Salmonella-killing in HIV-infected African adults. Killing was restored by genetically shortening LPS from the target Salmonella or removing LPS-specific antibodies from serum. Complement-mediated killing of Salmonella by healthy serum is shown to be induced specifically by antibodies against outer membrane proteins. This killing is Lost when excess antibody against Salmonella LPS is added. Thus, our study indicates that impaired immunity against nontyphoidal Salmonella bacteremia in HIV infection results from excess inhibitory antibodies against Salmonella LPS, whereas serum killing of Salmonella is induced by antibodies against outer membrane proteins. 10.1126/science.1180346
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- 2010
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7. The porin OmpD from nontyphoidal Salmonella is a key target for a protective B1b cell antibody response
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Gil-Cruz, Cristina, Bobat, Saeeda, Marshall, Jennifer L., Kingsley, Robert A., Ross, Ewan A., Henderson, Ian R., Leyton, Denisse L., Coughlan, Ruth E., Khan, Mahmood, Jensen, Karina T., Buckley, Christopher D., Dougan, Gordon, MacLennan, Ian C.M., Lopez-Macias, Constantino, and Cunningham, Adam F.
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Porins -- Research ,Porins -- Physiological aspects ,Bacteremia -- Development and progression ,Bacteremia -- Care and treatment ,Bacteremia -- Research ,Salmonella typhimurium -- Physiological aspects ,Salmonella typhimurium -- Research ,Science and technology - Abstract
Invasive nontyphoidal Salmonella (NTS), including Salmonella typhimurium (STm), are major yet poorly-recognized killers of infants in sub-Saharan Africa. Death in these children is usually associated with bacteremia, commonly in the absence of gastrointestinal symptoms. Evidence from humans and animal studies suggest that severe infection and bacteremia occur when specific Ab is lacking. Understanding how Ab responses to Salmonella are regulated will help develop vaccines against these devastating infections. STm induces atypical Ab responses characterized by prominent, accelerated, extrafollicular T-independent (TI) Ab against a range of surface antigens. These responses develop without concomitant germinal centers, which only appear as infection resolves. Here, we show STm rapidly induces a population of TI [B220.sup.+][CD5.sup.-] B1b cells during infection and TI Ab from B1b cells targets the outer membrane protein (Omp) porins OmpC, OmpD and OmpF but not flagellin. When porins are used as immunogens they can ablate bacteremia and provide equivalent protection against STm as killed bacterial vaccine and this is wholly B cell-dependent. Furthermore Ab from porin-immunized chimeras, that have B1b cells, is sufficient to impair infection. Infecting with porin-deficient bacteria identifies OmpD, a protein absent from Salmonella Typhi, as a key target of Ab in these infections. This work broadens the recognized repertoire of TI protein antigens and highlights the importance of Ab from different B cell subsets in controlling STm infection. OmpD is a strong candidate vaccine target and may, in part, explain the lack of cross-protection between Salmonella Typhi and STm infections. B cells | vaccines
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- 2009
8. Homeostatic cell-cycle control by BLyS: induction of cell-cycle entry but not [G.sub.1]/S transition in opposition to [p18.sup.INK4c] and [p27.sup.Kip1]
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Huang, Xiangao, Di Liberto, Maurizio, Cunningham, Adam F., Kang, Lin, Cheng, Shuhua, Ely, Scott, Liou, Hsiou-chi, MacLennan, Ian C.M., and Chen-Kiang, Selina
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Homeostasis -- Control ,Cell cycle -- Analysis ,Science and technology - Abstract
Cell-cycle entry is critical for homeostatic control in physiologic response of higher organisms but is not well understood. The antibody response begins with induction of naive mature B cells, which are naturally arrested in [G.sub.0]/[G.sub.1] phase of the cell cycle, to enter the cell cycle in response to antigen and cytokine. BLyS (BAFF), a cytokine essential for mature B cell development and survival, is thought to act mainly by attenuation of apoptosis. Here, we show that BLyS alone induces cell-cycle entry and early [G.sub.1] cell-cycle progression, but not S-phase entry, in opposition to the cyclin-dependent kinase inhibitors [p18.sup.INK4c]. Independent of its survival function, BLyS enhances the synthesis of cyclin D2, in part through activation of NF-[sub.[kappa].B], as well as CDK4 and retinoblastoma protein phosphorylation. By convergent activation of the same cell-cycle regulators in opposition to [p18.sup.INK4c], B cell receptor signaling induces cell-cycle entry and G1 progression in synergy with BLyS, but also DNA replication. The failure of BLyS to induce S-phase cell-cycle entry lies in its inability to increase cyclin E and reduce [p27.sup.Kip1] expression. Antagonistic cell-cycle regulation by BLyS and [p18.sup.INK4c] is functionally linked to apoptotic control and conserved from B cell activation in vitro to antibody response in vivo, further indicating a physiologic role in homeostasis. BAFF | cyclin D2 | cyclin E | cyclin-dependent kinase | B cell receptor signaling
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- 2004
9. Mycobacterial stationary phase induced by low oxygen tension: cell wall thickening and localization of the 16-kilodalton alpha-crystallin homolog
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Cunningham, Adam F. and Spreadbury, Claire L.
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Mycobacterium tuberculosis -- Research ,Dormancy (Biology) -- Research ,Bacterial cell walls -- Analysis ,Biological sciences - Abstract
Research was conducted to analyze the ability of Mycobacterium tuberculosis bacteria to survive a state of dormancy. This study required the culture of M. tuberculosis H37Rv and Mycobacterium bovis BCG under anaerobic, microaerobic and aerobic conditions. Observations of the bacteria's ultrastructural morphology revealed a thickening of the cell wall outer layer of the the cultured bacilli excepted for the aerobically cultured ones.
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- 1998
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