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4 results on '"Conley, Nicholas"'

1. Azido push-pull fluorogens photoactivate to produce bright fluorescent labels

Author

Lord, Samuel J., Lee, Hsiao-lu D., Samuel, Reichel, Weber, Ryan, Liu, Na, Conley, Nicholas R., Thompson, Michael A., Twieg, Robert J., and Moerner, W. E.

Subjects
Charge transfer -- Analysis, Azides -- Electric properties, Azides -- Chemical properties, Chemicals, plastics and rubber industries
Published
2010

2. Cy3-Cy5 covalent heterodimers for single-molecule photoswitching

Author

Conley, Nicholas R., Biteen, Julie S., and Moerner, W.E.

Subjects
Carbon compounds -- Chemical properties, Carbon compounds -- Electric properties, Chemicals, plastics and rubber industries
Abstract

The commercially available starting materials were used to characterize the covalent heterodimers of the Cy3 and Cy5 fluorophores at the single-molecule level. The covalent Cy3-Cy5 heterodimers were found to eliminate the need for probabilistic methods of situating the Cy3 and Cy5 in close proximity to enable photoswitching.

Published
2008

3. A photoactivatable push-pull fluorophore for single-molecule imaging in live cells

Author

Lord, Samuel J., Conley, Nicholas R., Lee, Hsiao-Iu D., Samuel, Reichel, Na Liu, Twieg, Robert J., and Moerner, W.E.

Subjects
Photochemistry -- Analysis, Absorption -- Analysis, Molecular mechanics -- Analysis, Chemistry
Abstract

A new, bright photoactivatable organic fluorophore is described that can be imaged at the single-molecule level in living cells. The azido-DCDHF fluorogen is an example of a rich new class of photoactivatable molecules, which is a powerful tool for single-molecule studies in the chemically and optically complex medium of the cell.

Published
2008

4. Bulk and single-molecule characterization of an improved molecular beacon utilizing H-dimer excitonic behavior

Author

Conley, Nicholas R., Pomerantz, Andrea Kurtz, Hui Wang, Twieg, Robert J., and Moerner, W.E.

Subjects
Fluorescence -- Analysis, Furans -- Structure, Furans -- Chemical properties, Furans -- Optical properties, Methyl groups -- Structure, Methyl groups -- Chemical properties, Methyl groups -- Optical properties, Bleaching -- Analysis, Chemicals, plastics and rubber industries
Abstract

A pair of fluorophores in the new dicyanomethylenedihydrofuran (DCDHF) dye family was used to design and characterize a new fluorescent probe for nucleic acid detection, referred as a self-quenched intramolecular dimer (SQuID) molecular beacon (MB).

Published
2007

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