1. Isolation and characterization of polymorphic microsatellite DNA markers from an Amazonian white-sand vegetation specialist bird, Xenopipo atronitens (Aves: Pipridae)
- Author
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Gubili, Chrysoula, Ritter, Camila Duarte, Motta, Jessica, Farias, Izeni P., Bates, John, Canton, Roberta, Capurucho, Joao M.G., Cornelius, Cintia, Feldheim, Kevin A., and Ribas, Camila C.
- Subjects
Applied Biosystems Inc. ,Promega Corp. ,Genetic markers -- Chemical properties ,DNA -- Chemical properties ,Biotechnology industry -- Chemical properties ,Biological sciences - Abstract
Thirteen novel polymorphic microsatellite loci were isolated and characterized from the Amazonian Black Manakin, Xenopipo atronitens. The loci were screened using 30 samples from Brazil. A total of 13 loci were variable with number of alleles per locus ranging from 2 to 17, whilst the observed and expected heterozygosities varied from 0.067-0.900 and 0.310-0.910, respectively. Five loci deviated significantly from the Hardy-Weinberg equilibrium, whereas one pair of loci deviated significantly from linkage disequilibrium. The set of markers will be a useful tool for future population genetic and kinship studies of X. atronitens, an Amazonian white-sand vegetation specialist. Received 24 September 2015. Accepted 12 January 2016. Key words: Amazon, Black Manakin, campina, nuclear markers, Xenopipo atronitens., INTRODUCTION The Black Manakin, Xenopipo atronitens (Cabanis, 1847) occurs mainly in white-sand vegetation habitats throughout the Amazon basin (Fig. 1; Stotz et al. 1996, Ridgely and Tudor 2009, Borges et [...]
- Published
- 2016