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ABSTRACT The study aimed to evaluate the influence of silver nitrate on the in vitro flowering of Capsicum frutescens cv. Cayenne associated with different light spectra and gas exchange. After 36 days of germination in MS medium, two seedlings previously germinated, were subjected to experiments in glass flasks containing two types of culture medium: I) MS culture medium; or II) Culture medium with the addition of 40 µM of AgNO3. The explants were maintained in a growth room with a 16-hour photoperiod, temperature of 25 ± 2 °C, light intensity (72 μmol m-2 s-1) from different light spectra. The experiment was conducted in a completely randomized design, 4 x 2 factorial scheme, with eight replicates, and the experimental unit composed of two seedlings. After 74 days of in vitro cultivation, the following parameters were evaluated: number of bifurcations, number of oxidized buds, number of open buds, number of green buds, number of aborted buds, number of leaves, root length, shoot length, total fresh and dry mass of shoots, and total fresh and dry mass of root. Thus, the combination of light spectrum, addition of gas exchange and AgNO3 favors the growth and development of Capsicum frutescens cv. Cayenne.

Utilizing quantum dot (QD) luminescent films as a greenhouse covering material is an innovative method of modifying the greenhouse light spectrum. The QD films convert a portion of high-energy ultraviolet and blue photons to lower-energy photons. Previous research has shown that the application of QD films in greenhouses led to improved crop yields of red lettuce and tomatoes. However, the underlying mechanism of the yield increases has not been fully explored. We quantified the effects of solar spectral shifts attributable to QD films on plant morphology, radiation capture, and, subsequently, crop yield. Green and red leaf lettuces and basil were grown in a greenhouse under four treatments: regular-concentration QD film (reg QD film); high-concentration QD film (high QD film); color-neutral polyethylene (PE) film; and control treatment without any films. Compared to the reg QD film, the high QD film converted a higher fraction of blue photons into longer-wavelength photons, resulting in enhanced leaf expansion, stem elongation, and shoot fresh weight of red lettuce and basil compared with those grown under the PE film without spectral modifications. No significant growth differences were observed between the control and high QD film treatments of red lettuce and basil despite a 23% reduction in the average daily light integral (DLI) under the high QD film treatment. Compared to that grown under the control treatment, green lettuce grown under the high QD film treatment had a similar total leaf area but reduced shoot biomass; this was likely associated with reductions in leaf thickness and chlorophyll content. In contrast, the red lettuce showed more pronounced leaf expansion and reduced leaf anthocyanin content under the high QD film, which likely helped to offset the reduction in DLI. Overall, our results indicated that modifying the solar spectrum with QD films as greenhouse covering material could result in improved crop radiation capture and yield in greenhouse production of lettuce and basil. However, the spectral shifts caused by the QD films may affect crop quality attributes, such as anthocyanin levels and the production of other beneficial secondary metabolites. This effect on crop quality should be carefully considered and requires further study.

Water colour remote sensing is a valuable tool for assessing bio-optical and biogeochemical parameters across the vast extent of the Amazon River Continuum (ARC). However, accurate retrieval depends on selecting the best atmospheric correction (AC). Four AC processors (Acolite, Polymer, C2RCC, OC-SMART) were evaluated against in situ remote sensing reflectance (Rrs) measurements. K-means classification identified four optical water types (OWTs) that are affected by the ARC. Two OWTs showed seasonal differences in the Lower Amazon River, influenced by the increase in suspended sediment concentration with river discharge. The other OWTs in the Amazon River Plume are dominated by phytoplankton or by a mixture of optically significant constituents. The Quality Water Index Polynomial method used to assess the quality of in situ and orbital Rrs had a high failure rate when the Apparent Visible Wavelength was >580 nm for in situ Rrs. OC-SMART Rrs products showed better spectral quality compared to Rrs derived from other AC processors evaluated in this study. These results improve our understanding of remotely sensing very turbid waters, such as those in the Amazon River Continuum.

Variations in the productivity of subnivean vegetation can substantially affect the ecology of regions more susceptible to increasing warming levels and lead to significant feedback effects on the global climate. Due to its importance, this topic is at the center of a broad scope of interdisciplinary studies supported by field and remote sensing observations. However, the current knowledge about environmental factors affecting the penetration of photosynthetically active radiation (PAR) through snow is still constrained by the paucity of transmittance data. In this work, we aim to further the understanding about these interconnected processes. We conduct a systematic investigation about the effects of independent and combined changes in key nivological characteristics, namely thickness, saturation, density and grain size, on snow transmittance in the photosynthetic spectral domain. Our investigation is carried out through controlled in silico (computational) experiments supported by measured radiometric data. Its outcomes unveil fundamental quantitative and qualitative trends related to the role played by these nivological characteristics on the spectral quality of transmitted PAR, which is quantified in terms of red to blue (R/B), red to far-red (R/FR) and blue to far-red (B/FR) ratios. These trends include increases in the R/B ratio as well as decreases in the R/FR and B/FR ratios following thickness reductions or grain size increases, with opposite variations in these ratios being observed for saturation or density increases. Accordingly, the pairing of our findings with in situ and remotely collected information contributes to cement the scientific foundation required for the effective assessment of cause-effect loops linking accentuated vegetation greening to accelerated rates of snow cover recession.

The spectral quality of supplemental greenhouse lighting can directly influence aroma volatiles and secondary metabolic resource allocation (i.e., specific compounds and classes of compounds). Research is needed to determine species-specific secondary metabolic responses to supplemental lighting (SL) sources with an emphasis on variations in spectral quality. The primary objective of this experiment was to determine the impact of supplemental narrowband blue (B) and red (R) LED lighting ratios and discrete wavelengths on flavor volatiles in hydroponic basil (Ocimum basilicum var. Italian Large Leaf). A natural light (NL) control and different broadband lighting sources were also evaluated to establish the impact of adding discrete and broadband supplements to the ambient solar spectrum. Each SL treatment provided 8.64 mol.m-2.d-1 (100 µmol.m-2.s-1, 24 h.d-1) photon flux. The daily light integral (DLI) of the NL control averaged 11.75 mol.m-2.d-1 during the growth period (ranging from 4 to 20 mol.m-2.d-1). Basil plants were harvested 45 d after seeding. Using GC-MS, we explored, identified, and quantified several important volatile organic compounds (VOCs) with known influence on sensory perception and/or plant physiological processes of sweet basil. We found that the spectral quality from SL sources, in addition to changes in the spectra and DLI of ambient sunlight across growing seasons, directly influence basil aroma volatile concentrations. Further, we found that specific ratios of narrowband B/R wavelengths, combinations of discrete narrowband wavelengths, and broadband wavelengths directly and differentially influence the overall aroma profile as well as specific compounds. Based on the results of this study, we recommend supplemental 450 and 660 nm (± 20 nm) wavelengths at a ratio of approximately 10B/90R at 100-200 µmol.m-2.s-1, 12-24 h.d-1 for sweet basil grown under standard greenhouse conditions, with direct consideration of the natural solar spectrum and DLI provided for any given location and growing season. This experiment demonstrates the ability to use discrete narrowband wavelengths to augment the natural solar spectrum to provide an optimal light environment across variable growing seasons. Future experiments should investigate SL spectral quality for the optimization of sensory compounds in other high-value specialty crops.

Basella alba L. (Malabar spinach) is a non-conventional food plant (PANC) with high nutritional value and economic potential. Light is a critical factor in plant growth, acting directly on the photosynthetic process. The photoselective meshes combine the physical protection of plants with the selective filtering of solar radiation to promote physiological responses. This work evaluates the growth of B. alba under different light environments. The experiment occurred in a greenhouse at the Center for Agrarian, Environmental and Biological Sciences of the Federal University of Recôncavo da Bahia in a completely randomized design with plots comprising one useful plant and five replications. The plants were grown in three light environments: red ChromatiNet mesh, aluminet-thermo reflective mesh (both with 50% irradiance), and under full sun. The red mesh increased the root dry matter mass and stem diameter. The aluminet mesh increased the average number of leaves by about 50% compared to the plants cultivated under full sun. The results show that the cultivation of B. alba in different light environments promotes morphophysiological changes with positive increments in growth.

Archival aerial photographs obtained in the 20th century play a special role in the process of upgrading the register of land and buildings. These photographs are the only resource presenting credible information on the coverage and use of land in a high degree of detail. In this article, particular attention is paid to the aspect of the spectral resolution of archival aerial photographs. Preliminary research was conducted into the assessment of the impact of simulations of new spectral bands with high spatial resolution for archival photographs upon the interpretation process of such data. The proposed simulation method of new bands is based on the integration of archival monochromatic aerial images with archival multispectral satellite imagery. Visual and quantitative comparative analysis of monochromatic imagery and of enhanced images obtained by application of the simulation process was performed. The results of this research unanimously confirmed the improved interpretation possibilities of archival aerial photographs, associated not only with the assignment of colours but also with the reconstruction of spectral information for the arable and afforested land photographed.

Brosimum gaudichaudii is a plant species with medicinal relevance due to its furanocoumarin accumulation. The accumulation of these compounds in the root promotes predatory extractivism, which threatens the conservation of the species. In addition, little is known about the conditions for culturing of this species in vitro. The present study aimed to investigate how the application of different spectra of LEDs (white, blue, red, and combinations of blue and red at 1:1 and 3:1 ratios) can impact the morphophysiological and biochemical characteristics of B. gaudichaudii under different in vitro conditions. To evaluate the production of furanocoumarins in its leaves, which are easy-to-collect perennial organs, we cultured nodal segments in 50-mL tubes with MS medium under 100 μmol m−2 s−1 light and a photoperiod of 16 h for 50 days. We then submitted the seedlings biometric, anatomical, biochemical, and physiological evaluations. The different spectral qualities influenced several characteristics of the seedlings. Plants grown under red light showed greater stem elongation and larger and thinner leaves, strategies aimed at capturing a higher ratio of radiant energy. Exposure to the blue/red ratio of 1:1 induced increases in the concentration of the furanocoumarin psoralen, probably due to the diversion of carbon from primary metabolism, which resulted in lower growth. Cultivation under blue light or blue:red light at 3:1 triggered anatomical and physiological changes that led to higher production of secondary metabolites in the leaves, and at the 3:1 ratio, the seedlings also had a high growth rate. These results highlight the fundamental role of light in stimulating the production of secondary metabolites, which has important implications for the production of compounds of interest and indirect consequences for the conservation of B. gaudichaudii.

A fundamental limitation of the spectral response of laser-pumped fluorescent nanostructured media was considered in terms of a probabilistic model establishing the relationship between the enhancement factor of a spectral quality and characteristic propagation and amplification scales of pump light and fluorescence in the medium. It was shown that the minimum spectral width of fluorescent response of the pumped medium is limited by competition between the stimulated emission and radiation losses in microscopic fluorescence emitters associated with the speckles randomly modulating the pumping light field. Theoretical results were compared to the experimental data on the spectral properties of the fluorescent response of laser-pumped nanostructured “anatase nanoparticles—dye solutions” systems with various structural and optical properties. Rhodamine 6G and 4-(dicyanomethylene)-2-methyl-6-(p-dimethylaminostyryl)-4H-pyran (DCM) were applied as fluorescent components in the examined systems with various scatter volume fractions, which were pumped by pulse-periodic laser radiation with various intensities at 532 nm. Comparison showed a fair agreement between the theoretical and experimental results.

The effect of continuous lighting (CL, 24 h) and light spectrum on growth and nutritional quality of arugula (Eruca sativa), broccoli (Brassica oleracea var. italic), mizuna (Brassica rapa. var. nipposinica), and radish (Raphanus sativus var. radicula) were investigated in growth chambers under light-emitting diode (LED) and fluorescent lighting. Microgreens were grown under four combinations of two photoperiods (16 h and 24 h) providing daily light integral (DLI) of 15.6 and 23.3 mol m−2 day−1, correspondingly) with two light spectra: LED lamps and fluorescent lamps (FLU). The results show that fresh and dry weights as well as leaf mass per area and robust index of harvested arugula, broccoli, mizuna, and radish seedlings were significantly higher under CL compared to 16 h photoperiod regardless of light quality. There were no visible signs of leaf photodamage. In all CL-treated plants higher chlorophyll a/b and carotenoid-to-chlorophyll ratios were observed in all plants except mizuna. CL treatment was beneficial for anthocyanin, flavonoid, and proline accumulation. Higher activities of antioxidant enzymes (catalase, superoxide dismutase, ascorbate peroxidase, and guaiacol peroxidase) were also observed in CL-treated plants. In most cases, the effects were more pronounced under LED lighting. These results indicate that plants under mild oxidative stress induced by CL accumulated more non-enzymatic antioxidants and increased the activities of antioxidant enzymes. This added nutritional value to microgreens that are used as functional foods providing health benefits. We suggest that for arugula, broccoli, mizuna, and radish, an LED CL production strategy is possible and can have economic and nutritional benefits.

Spectral characteristics of solar radiation have a major role in plant growth and development and the overall metabolism, including secondary metabolism, which is important for the accumulation of health-promoting phytochemicals in plants. The primary focus of this study was to determine the effect of spectral characteristics of solar radiation on the nutritional quality of lettuce (Lactuca sativa L., cv. red leaf ‘New Red Fire’ and green leaf ‘Two Star’ and tomato (Solanum lycopersicum L., cv. BHN-589) grown in high tunnels in relation to the accumulation of essential nutrients and phytochemicals. Solar spectrum received by crops was modified using photo-selective poly covers. Treatments included commonly used standard poly, luminescence poly (diffuse poly), clear poly, UV blocking poly, exposure of crops grown under the standard poly to full sun 2 weeks prior to harvest (akin to movable tunnel), and 55% shade cloth on the standard poly. All the poly covers and shade cloth reduced the PAR levels in the high tunnels, and the largest reduction was by the shade cloth, which reduced the solar PAR by approximately 48%. Clear poly allowed the maximum UV-A and UV-B radiation, while standard poly allowed only a small fraction of the solar UV-A and UV-B (between 15.8% and 16.2%). Clear poly, which allowed a higher percentage of solar UV-A (60.5%) and UV-B (65%) than other poly covers, increased the total phenolic concentration and the antioxidant capacity in red leaf lettuce. It also increased the accumulation of flavonoids, including quercetin-3-glucoside, luteolin-7-glucoside, and apigenin-3-glucoside in red leaf lettuce, compared to the standard poly. Brief exposure of crops grown in high tunnels to full sun prior to harvest produced the largest increase in the accumulation of quercetin-3-glucoside, and it also resulted in an increase in luteolin-7-glucoside and apigenin-3-glucoside in red leaf lettuce. Thus, clear poly and brief exposure of red leaf lettuce to the full sun, which can increase UV exposure to the plants, produced a positive impact on its nutritional quality. In contrast, shade cloth which allowed the lowest levels of solar PAR, UV-A and UV-B relative to the other poly covers had a negative impact on the accumulation of the phenolic compounds in red leaf lettuce. However, in green leaf lettuce, luminesce poly, clear poly, UV-block poly, and shade treatments increased the accumulation of many essential nutrients, including protein, magnesium, and sulfur in green leaf lettuce compared to the standard poly. Poly cover treatments including shade treatment did not affect the accumulation of either carotenoids (lutein, β-carotene, and lycopene) or essential nutrients in mature tomato fruits. The results show that clear poly cover can enhance the accumulation of many phenolic compounds in red leaf lettuce, as does the brief exposure of the crop to the full sun prior to harvest. Thus, UV radiation plays an important role in the accumulation of phenolic compounds in red leaf lettuce while the overall spectral quality of solar radiation has a significant influence on the accumulation of essential nutrients in green leaf lettuce.

Plant biomass is largely dictated by the total amount of light intercepted by the plant [daily light integral (DLI) — intensity × photoperiod]. Continuous light (CL, 24 h lighting) has been hypothesized to increase plant biomass and yield if CL does not cause any injury. However, lighting longer than 18 h causes leaf injury in tomato characterized by interveinal chlorosis and yield is no longer increased with further photoperiod extension in tomatoes. Our previous research indicated the response of cucumbers to long photoperiod of lighting varies with light spectrum. Therefore, we set out to examine greenhouse tomato production under supplemental CL using an alternating red (200 µmol m−2 s−1, 06:00–18:00) and blue (50 µmol m−2 s−1, 18:00–06:00) spectrum in comparison to a 12 h supplemental lighting treatment with a red/blue mixture (200 µmol m−2 s−1 red + 50 µmol m−2 s−1 blue, 06:00–18:00) at the same DLI. Our results indicate that tomato plants grown under supplemental CL using the red and blue alternating spectrum were injury-free. Furthermore, parameters related to photosynthetic performance (i.e., Pnmax, quantum yield, and Fv/Fm) were similar between CL and 12 h lighting treatments indicating no detrimental effect of growth under CL. Leaves under CL produced higher net carbon exchange rates (NCER) during the subjective night period (18:00–06:00) compared to plants grown under 12 h lighting. Notably, 53 days into the treatment, leaves grown under CL produced positive NCER values (photosynthesis) during the subjective night period, a period typically associated with respiration. At 53 days into the growth cycle, it is estimated that leaves under CL will accumulate approximately 800 mg C m−2 more than leaves under 12 h lighting over a 24 h period. Leaves grown under CL also displayed similar diurnal patterns in carbohydrates (glucose, fructose, sucrose, and starch) as leaves under 12 h lighting indicating no adverse effects on carbohydrate metabolism under CL. Taken together, this study provides evidence that red and blue spectral alternations during CL allow for injury-free tomato production. We suggest that an alternating spectrum during CL may alleviate the injury typically associated with CL production in tomato.

High-tech greenhouses and artificial light applications aim to improve food production, in line with one of the sustainable development goals of the UN Agenda 2030, namely, “zero hunger”. In the past, the incandescent lamps have been used for supplementary lighting (SL) at higher latitudes to increase greenhouse production during the dark season. Light-emitting diodes (LED) have been replacing gas discharge and incandescent lamps, and their development is expanding SL applications in different agricultural scenarios (e.g., urban farming, middle latitudes). In fact, recent research on LED applications in Mediterranean greenhouses have produced encouraging results. Since middle latitudes have a higher daily light integral (DLI) than higher latitudes in the dark season and climate conditions influence the installed power load of greenhouses, LED installation and management in Mediterranean greenhouses should be different and less expensive in terms of investment and energy consumption. Accordingly, the aim of this review is to outline the state of the art in LED applications and development, with a focus on latitude-related requirements. Tomato was used as a representative crop.

The growing demand for high-quality imaging data and the current technological limitations of imaging sensors require the development of techniques that combine data from different platforms in order to obtain comprehensive products for detailed studies of the environment. To meet the needs of modern remote sensing, the authors present an innovative methodology of combining multispectral aerial and satellite imagery. The methodology is based on the simulation of a new spectral band with a high spatial resolution which, when used in the pansharpening process, yields an enhanced image with a higher spectral quality compared to the original panchromatic band. This is important because spectral quality determines the further processing of the image, including segmentation and classification. The article presents a methodology of simulating new high-spatial-resolution images taking into account the spectral characteristics of the photographed types of land cover. The article focuses on natural objects such as forests, meadows, or bare soils. Aerial panchromatic and multispectral images acquired with a digital mapping camera (DMC) II 230 and satellite multispectral images acquired with the S2A sensor of the Sentinel-2 satellite were used in the study. Cloudless data with a minimal time shift were obtained. Spectral quality analysis of the generated enhanced images was performed using a method known as “consistency” or “Wald’s protocol first property”. The resulting spectral quality values clearly indicate less spectral distortion of the images enhanced by the new methodology compared to using a traditional approach to the pansharpening process.

(1) Background: We assessed the impact of high tunnel coverings and harvest maturity (breaker and light red) on antioxidant capacity, ascorbic acid (AsA), lycopene, β–carotene, and phenolic compound (flavonoid and phenolic acid) accumulation in tomatoes (Solanum lycopersicum) at harvest and postharvest. (2) Methods: The two-year study in Olathe, KS, included six different coverings: a standard polyethylene (standard poly), diffuse poly (diffuse), clear poly (clear), UV-A/UV-B blocking poly (block), 55% shade cloth + standard poly (shade), and removal of standard poly two weeks prior to harvest (movable). (3) Results: Antioxidant capacity increased in fruit grown under the clear covering, compared to the shade covering (p < 0.05); similarly, AsA accumulation increased under the standard and clear coverings, relative to the movable and shade coverings (p < 0.001). Postharvest, at the point of consumption (POC), rutin increased in fruit harvested at light red stage versus breaker stage (p < 0.001), and chlorogenic acid increased in light red harvested fruit by 60% under movable, 55% under shade, and 43% under block covering than breaker harvested fruit (p < 0.01). (4) Conclusions: Based on these results, we conclude that both high tunnel covering and postharvest maturation alter antioxidant capacity, AsA, lycopene, and phenolic compound accumulation profiles by the POC.

In this study, the results from a round-robin test of hyperspectral imaging systems are presented and analyzed. Fourteen different pushbroom hyperspectral systems from eight different institutions were used to acquire spectral cubes from the visible, near infra-red and short-wave infra-red regions. Each system was used to acquire a common set of targets under their normal operating conditions with the data calibrated and processed using the standard processing pipeline for each system. The test targets consisted of a spectral wavelength standard and of a custom-made pigment panel featuring Renaissance-era pigments frequently found in paintings from that period. The quality and accuracy of the resulting data was assessed with quantitative analyses of the spectral, spatial and colorimetric accuracy of the data. The results provide a valuable insight into the accuracy, reproducibility and precision of hyperspectral imaging equipment when used under routine operating conditions. The distribution and type of error found within the data can provide useful information on the fundamental and practical limits of such equipment when used for applications such as spectral classification, change detection, colorimetry and others.

Commonly used image fusion techniques generally produce good results for images obtained from the same sensor, with a standard ratio of spatial resolution (1:4). However, an atypical high ratio of resolution reduces the effectiveness of fusion methods resulting in a decrease in the spectral or spatial quality of the sharpened image. An important issue is the development of a method that allows for maintaining simultaneous high spatial and spectral quality. The authors propose to strengthen the pan-sharpening methods through prior modification of the panchromatic image. Local statistics of the differences between the original panchromatic image and the intensity of the multispectral image are used to detect spatial details. The Euler’s number and the distance of each pixel from the nearest pixel classified as a spatial detail determine the weight of the information collected from each integrated image. The research was carried out for several pan-sharpening methods and for data sets with different levels of spectral matching. The proposed solution allows for a greater improvement in the quality of spectral fusion, while being able to identify the same spatial details for most pan-sharpening methods and is mainly dedicated to Intensity-Hue-Saturation based methods for which the following improvements in spectral quality were achieved: about 30% for the urbanized area and about 15% for the non-urbanized area.

Diatoms are important phototrophs in the worlds’ oceans contributing approximately 40% of the global primary photosynthetic production. This is partially explained by their capacity to exploit environments with variable light conditions, but there is limited knowledge on how diatoms cope with changes in the spectral composition and intensity of light. In this study, the influence of light quality and high irradiance on photosynthesis in the centric diatom Coscinodiscus granii was investigated with microscopic imaging and variable chlorophyll fluorescence techniques. Determination of the wavelength-dependent functional absorption cross-section of photosystem (PS) II revealed that absorption of blue light (BL) and red light (RL) was 2.3-fold and 0.8-fold that of white light (WL), respectively. Hence, BL was more efficiently converted into photo-chemical energy. Excessive energy from BL was dissipated via non-photochemical quenching (NPQ) mechanisms, while RL apparently induced only negligible NPQ even at high irradiance. A dose dependent increase of cells exhibiting an altered chloroplast distribution was observed after exposure to high levels of BL and WL, but not RL. However, no effective quantum yield of PSII was measured in the majority of cells with an altered chloroplast distribution, and positive Sytox green® death staining confirmed that most of these cells were dead. We conclude that although Coscinodiscus granii can sustain high irradiance it does not perform chloroplast high-light avoidance movements for photo-protection.