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The use of transferable cross species/ genera SSR markers is considered as cost effective strategy to ensure availability of markers in genomic resources of cotton. Forty one of 88 SSR primer pairs constitutes 46.59 % [24 from safflower i.e 27.27% and 17 from pulses i.e 19.32%] were found transferable as they amplified SSR priming genomic regions of both the genotypes. Out of these 88 markers, 27 (30.68%) were shown monomorphism and 14 (15.91%) were shown polymorphism. Among amplified markers, 45.83% and 17.65% polymorphism was observed by these heterolgous markers of safflower and pulses respectively.

ABSTRACT: Brycon orbignyanus, popularly known in Brazil as piracanjuba, is a fish with great economic value but whose natural population drastically decreased in number during the last years. In this context, genetic variability studies of natural stocks and in restocking programs are fundamental for the adoption of conservation measures. Current analysis verifies the cross-amplification of heterologous primers in B. orbignyanus. Fifty-two primers of the species Brycon opalinus, Brycon hilarii, Brycon insignis, Prochilodus sp., Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus were tested. Primers with the best reproducibility were applied to a sample of 20 individuals and the genetic parameters were calculated. Nine primers provided good results for cross-amplification with B. orbignyanus, involving (BoM5 and BoM13) of Brycon opalinus, (Bh5, Bh6, Bh8, Bh13 and Bh16) of Brycon hilarii, (Bc48-10) of Brycon insignis and (Par80) of Prochilodus argenteus. Primers of Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus failed to provide amplification or provided non-specificity. Results demonstrated the possibility of using primers of different species and genera of B. orbignyanus, facilitating genetic studies on the species.