1. Effect and mechanism of TGF-β on macrophage-derived cancer-associated fibroblasts in bladder can-cer tissue
- Author
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PU Shi, SHEN Chengquan, HU Ding, ZHAO Xinzhao, QIN Ruize, LIU Changxue, WANG Yonghua
- Subjects
transforming growth factor beta ,cancer-associated fibroblasts ,urinary bladder neoplasms ,tumor-asso-ciated macrophages ,immune tolerance ,cd8-positive t-lymphocytes ,cell infiltration ,Medicine - Abstract
Objective To investigate the effect and mechanism of TGF-β on macrophage-derived cancer-associated fibroblasts (CAFs) in bladder cancer tissue. Methods The Kaplan-Meier method was used to investigate the relationship between the expression of α-SMA+CD68+CAF and overall survival (OS) in bladder cancer; immunofluorescence assay was used to observe the infiltration of α-SMA+CD68+CAF in bladder cancer tissue; Western blot was used to observe the inductive effect of TGF-β on α-SMA+CD68+CAF in vitro. A mouse model of bladder cancer was established, and immunofluorescence assay and immunohistochemistry were used to observe the inductive effect of TGF-β on α-SMA+CD68+CAF and its effect on the infiltration of CD8+T cells in mice with bladder cancer. Results The expression of α-SMA was positively correlated with that of CD68 in bladder can-cer tissue, and the patients with a high expression level of α-SMA+CD68+CAF tended to have a poorer prognosis (χ2=9.05,P
- Published
- 2024
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