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This study aims to identify key regulators of paraptosis in gastric cancer (GC) and explore their potential in guiding therapeutic strategies, especially in stomach adenocarcinoma (STAD). Genes associated with paraptosis were identified from the references and subjected to Cox regression analysis in the TCGA-STAD cohort. Using machine learning models, LPAR1 consistently ranked highest in feature importance. Multiple sequencing data showed that LPAR1 was significantly overexpressed in cancer-associated fibroblasts (CAFs). LPAR1 expression was significantly higher in normal tissues, and ROC analysis demonstrated its discriminative ability. Copy number alterations and microsatellite instability were significantly associated with LPAR1 expression. High LPAR1 expression correlated with advanced tumor grades and specific cancer immune subtypes, and multivariate analysis confirmed LPAR1 as an independent predictor of poor prognosis. LPAR1 expression was associated with different immune response metrics, including immune effector activation and upregulated chemokine secretion. High LPAR1 expression also correlated with increased sensitivity to compounds, such as BET bromodomain inhibitors I-BET151 and RITA, suggesting LPAR1 as a biomarker for predicting drug activity. FOXP2 showed a strong positive correlation with LPAR1 transcriptional regulation, while increased methylation of LPAR1 promoter regions was negatively correlated with gene expression. Knockdown of LPAR1 affected cell growth in most tumor cell lines, and in vitro experiments demonstrated that LPAR1 influenced extracellular matrix (ECM) contraction and cell viability in the paraptosis of CAFs. These findings suggest that LPAR1 is a critical regulator of paraptosis in GC and a potential biomarker for drug sensitivity and immunotherapy response. This underscores the role of CAFs in mediating tumorigenic effects and suggests that targeting LPAR1 could be a promising strategy for precision medicine in GC.

In order to study the change rules of mineral composition, micro-structure evolution characteristics and their relationship with dynamic properties of granite after high temperature treatment, the high temperature tests on Beishan granite in Gansu Province, China, were carried out at 20−1 000 ℃. Microscopic images of granite after high temperature treatment were obtained by slicing technique and polarizing microscope. The evolution of mineral composition, mineral content and micro-structure characteristic parameters of granite with temperature were studied through mineral composition analysis, micro-cracks characteristic identification and parameters calculation. Subsequently, dynamic impact compression tests of high temperature treated granite were carried out by split Hopkinson pressure bar (SHPB) system, to analyze the effects of temperature, impact rate and micro-structure characteristics on dynamic peak stress. On this basis, the quantitative relationship between micro-structure characteristics and macroscopic dynamic properties was established. The results show that high temperature has a significant effect on the mineral composition, micro-structure and impact compressive strength of granite, and 600 ℃ is taken as the threshold temperature. The loading rate also significantly affects the impact compressive strength, and the larger the loading rate is, the less the influence of temperature on the macroscopic dynamic properties is. The evolution process of the micro-structure of granite after high temperature treatment is the internal mechanism for the change of its macroscopic dynamic properties with temperature. The microcrack characteristics have a significant correlation with the dynamic peak stress, and the average crack width is the main factor affecting the dynamic peak stress of granite.

Revise ISO 22768∶2017 based on China′s industrial standards, and expand the scope of application to latex. The effects of sample preparation method, gas flow rate, heating rate and sample amount on the determination results of glass transition temperature were investigated, and the precision of the method was established. The results showed that, when the drying method was selected, the gas flow rate was 20 mL/min, the sample amount was 5-20 mg, and the heating rate was 20 ℃/min, the method had good precision, the standard deviation of repeatability was less than 0.30 ℃ and the standard deviation of reproducibility was less than 1.01 ℃.

The contents of soap and organic acid in emulsion polymerized styrene-butadiene rubber determined by indicator titration and potentiometric titration in ISO 7781 were compared. The differences between the determination principles and results of the two methods were compared, and the accuracy of the results was investigated by standard addition experiment. The results showed that the results of potentiometric titration and indicator method were consistent. When testing the soap content in emulsion polymerized styrene-butadiene rubber, the indicator method was greatly affected by amine antioxidant and filling oil. But the potentiometric titration method was less affected by amine antioxidant and was not affected by filling oil, so the determination result was more accurate.

The curing characteristics and tensile properties of sulfur and mercaptan modified chloroprene rubber(CR) were evaluated using carbon black free formulation were studied .The results showed that the specific surface area of magnesium oxide(MgO) affected the curing rate of CR, with the increase of the specific surface area of MgO, the curing efficiency improved. The specific surface area of MgO also effected the tensile properties of vulcanizated CR. The carbon black free formulation A and formulation B were applicable to evaluate curing characteristics and tensile properties of mercaptan modified CR, and the evaluation results may not be compareable. The carbon black free formulation was suitable for evaluating the curing characteristics and tensile properties of sulfur modified and mercaptan modified CR, and the precision of the test results could meet the requirements of ASTM D 3190—2006.

In order to improve the precision and beam quality of a pump laser for a spin exchange relaxation free inertial measurement device, we applied one scheme to achieve the square wave modulation and power stability control of the pump laser and another one to obtain the uniform intensity distribution of the laser beam, in which the acousto-optic modulator (AOM) and proportion integration differentiation (PID) controller were used to achieve the former, and the freeform surface lens was designed and optimized to achieve the latter based on the TracePro software. In experiments, the first-order diffraction light beam coming through the AOM had a spot size of about 1.1 × 0.7 mm2, and a spherical vapor cell with a radius of 7 mm was placed behind the freeform surface lens. Results show that the uniformity of the reshaped intensity distribution is higher than 90% within the target area with a radius of 7 mm both in the simulation and the experiment, which ensure that the uniform laser beam covers the area of cell. On the other hand, the power stability of the pump laser is controlled to be less than 0.05%. Compared with traditional methods, the complicated calculation process in optical design is better solved, and a higher uniformity with slight energy loss is achieved.

The objective of this study was to investigate the flow dynamics and temperature characteristics under different reservoir operation scenarios and weather conditions in the river-reservoir system, which can be used to set scientific guidelines for river management and conservation planning strategies. The calibrated three-dimensional model provided simulated unsteady water surface elevation, temperature, velocity and discharge at different layers (depths) in different locations. A series of operation scenarios were modeled to understand and quantify formation, propagation, and disappearance of density currents that are resulted from combinations of daily repeated large release (DRLR) of different durations and solar heating. DRLRs (140 m3/s) with longer durations pushed the bottom cold water further downstream and maintained the bottom water temperature cooler. Variations of weather conditions (e.g., drops of air temperature and solar radiation) directly controled variations of bottom-layer water temperature. The daily drop rate of bottom temperature was related to the rate and duration of air temperature drop. Under the practice for the water turbines running at downstream, it clearly showed the shocking withdrawal and stopping effect from the downstream operation. The velocity for the whole cross-section were almost increased with same magnitude of about 0.1 m/s at GOUS and JML.