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Background. Currently, the use of an interdisciplinary approach based on a combination of traditional introduction methods and clonal micropropagation techniques makes it possible to solve one of the key problems of introduction – the establishment of bioresource collections consisting of selected plant accessions with valuable agronomic traits and resistance to unfavorable urban environments. Materials and methods. A plant introduction study resulted in identifying seven specimens of Malus niedzwetzkyana Dieck with resilience to urban environments, high rate of crown development, and longevity. They served as source material for the development of a clonal propagation protocol and in vitro preservation of selected genotypes of this species. Results. It was shown for M. niedzwetzkyana that the most favorable time for taking its plant material for introduction into in vitro culture is the beginning of the active growth of vegetative shoots after flowering. The most optimal sterilization technique for such plant material was a stepwise regime using alcohol, sodium hypochlorite, and silver nitrate: it provided from 50 to 70% of sterile explants and the maximum percentage of meristem proliferation. Combining 0.8 mg/L of benzylaminopurine (BAP) with 0.14 mg/L of indole-3-butyric acid (IBA) at the stage of microclonal propagation ensured a significant increase of the reproduction coefficient (on average up to 5.3 ± 0.7) and an improvement in morphometric parameters of microshoots; the maximum frequency of shoot proliferation was 100%. The yield of shoots adapted to ex vitro conditions was 90%. Conclusion. The developed clonal micropropagation protocol made it possible to introduce selected M. niedzwetzkyana forms into in vitro culture and reproduce them in order to set up a resource base for further fundamental and applied research into the system of the genus Malus Mill.

Aim: to present literature data on the importance of intra-abdominal hypertension in the pathogenesis of peritonitis. Key points. Secondary purulent peritonitis is a severe and fairly common form of abdominal infection with high mortality. According to statistics, hospitalization of patients with signs of local or diffuse peritonitis is more than 15 % among patients with acute surgical pathologies. One of the factors in the development of complications and an unfavorable prognosis for this disease is increased intra-abdominal pressure. To measure this indicator, a Foley catheter inserted into the bladder is used in medical practice. According to reference values, the optimal level of intra-abdominal pressure does not exceed 5 mmHg. A persistent increase in pressure of 12 mmHg and above indicates the development of intra-abdominal hypertension. An increase in the level of intra-abdominal pressure indicates the progression of the inflammatory destructive process in the abdominal cavity and contributes to the development of multiple organ failure with subsequent fatal outcome with untimely treatment. With an indicator exceeding 20 mmHg, there is a risk of developing abdominal compartment syndrome. This condition is characterized by increased pressure in two or more anatomical areas, which leads to decreased blood flow and subsequent tissue hypoxia. Intra-abdominal hypertension also increases the risk of developing postoperative peritonitis in patients who have undergone laparotomy. Conclusion. To predict the course of secondary diffuse purulent peritonitis, reduce the risk of complications and mortality, it is necessary to focus on measuring intra-abdominal pressure as a mandatory manipulation in surgical patients. Despite its general availability and ease of implementation, this technique allows assessing the severity of organ dysfunctions.

Introduction. The introduction of devices – analogues of GIS (hereinafter–- Gastro-intestinal simulator) is one of the current ways to develop in-vitro assessment of the quality of solid dosage forms. Testing on a physiologically relevant test device (hereinafter referred to as PRT) makes it possible to predict pharmacokinetic profiles due to more relevant conditions, including the use of biorelevant dissolution media, physiological volumes of the gastrointestinal tract, as well as transit between them.Aim. Conduct a study of cladribine tablets on a physiologically relevant tester in order to predict the behavior of the drug in the human gastrointestinal tract.Materials and methods. The objects of the study are "Mavenclad®, tablets, 10 mg" (series 2200754, expiration date until 04.2025, NERPHARMA, S.r.L., Italy) and "Cladribine, tablets, 10 mg" of domestic production with valid expiration date. During the study, the reagents necessary for the preparation of biorelevant dissolution media and quantitative determination by HPLC. Physiologically relevant test were carried out using an apparatus of our own production, consisting of a DT-6 dissolution tester (ERWEKA GmbH, Germany), a water bath equipped with a Thermomix WB-4 heating element (B. Braun, Germany), and peristaltic pumps (Kamoer, China). The quantitative content of released cladribine was assessed using a highly efficient liquid chromatograph "Khromatek-Kristall HPLC 2014" (ZAO SKB "Khromatek", Russia) using a validated method at a wavelength of 252 nm, analysis time – 7 min, column – Grace HPLC Column Platinum C18-EPS, 250 × 4.6 mm, 5 mm (Grace, USA), temperature – 35 °C, elution mode – isocratic (A : B 80 : 20), mobile phase A – 0.1 % H3PO4 solution, phase B – acetonitrile.Results and discussion. Profiles were obtained to assess the dynamics and degree of release of the studied drugs in various parts of the human gastrointestinal tract. Despite the expected degradation of cladribine in an acidic environment (pH1.2), under physiologically relevant conditions, the drug reached the third section (small intestine model) without degradation. Complete release of cladribine from the test and reference dosage forms was observed. Also, in the future, based on the data obtained, it is possible to predict pharmacokinetic profiles using physiologically based pharmacokinetic modeling approaches.Conclusion. A PSF study was conducted for the drugs "Mavenclad®, tablets, 10 mg" and "Cladribine, tablets, 10 mg". Quantitative determination was carried out by HPLC-UV method. The test results showed complete release of both drugs and reaching the intestinal tract, indicating the absence of degradation of cladribine in the region simulating the stomach.

The current paper has presented a detailed analysis of the production of winter barley over a long period, taking into account their sown areas, gross yields and productivity in farms of all categories in the Russian Federation and the Rostov region. The purpose of the study was to estimate the production and monitor the varietal composition of sown winter barley seeds in the Rostov region. In the region, the sown area occupied by winter barley annually amounts to more than 41.1 thousand hectares with a mean yield of 3.9 t/ha. An analysis of the sown winter barley varieties in the Rostov region was carried out according to the year they were included in the State List of the Russian Federation. As a result of study, there was found that there were 6–9 barley varieties included in the State List in the period up to 4 years; 4 ones in the period of 5–8 years; 5–6 ones in the period of 9–12 years; 4–6 ones in the period of more than 12 years. There was established that the largest area under winter barley was occupied by the varieties of local breeding of the FSBSI “ARC “Donskoy” (49.5–58.5 %), about 16.0–25.1 % of the area was occupied by the varieties of the Krasnodar breeding of the FSBSI “NGC named after P.P. Lukyanenko”. There was found out that the most popular varieties in the Rostov region over the past three years were the varieties ‘Erema’ (18.4–18.4 %), ‘Timofey’ (12.5–13.3 %), ‘Vivat’ (11.9–12.7 %), ‘Marusya’ (5.7–12.6 %), ‘Foks 1’ (1.9–3.6 %), ‘Karrera’ (8.7–13.2 %), ‘Iosif’ (7.4–10.2 %), ‘Dobrynya 3’ (1.8–2.9 %), ‘Samson’ (0.6–3.4 %), ‘Luran’ (0.8–4.5 %) and ‘Dostoiny’ (2.6–8.5 %). Thus, to improve the production of winter barley grain in the Rostov region, it is necessary to cultivate varieties that are most adapted to local conditions, have high productivity and grain quality, and are included in the State List for the North Caucasus region.

Guangzan Yu, Lulu Liu, Qian Ma, Fusheng Han, Hua He Cardiac Division of Emergency Intensive Care Unit, Beijing Anzhen Hospital, Capital Medical University, Beijing, 100029, People’s Republic of ChinaCorrespondence: Hua He, Email hehua430@163.comBackground: A large number of studies have demonstrated links between chronic obstructive pulmonary disease (COPD) and cardiovascular diseases (CVDs). However, the causal relationship between COPD and CVDs and the reverse causality remains divergent.Methods: Exposure and outcome data from the largest available genome-wide association studies were extracted for Mendelian randomization (MR) studies. Univariate MR analysis was performed using IVW as the primary analysis method, and multiple sensitivity analyses were used to enhance the robustness of the results. Furthermore, this was followed by mediation MR analysis of positive results after excluding confounding factors with multivariable MR analysis.Results: The MR estimation based on IVW method indicated a strong association between genetically determined COPD and heart failure (HF) (OR = 1.117, 95% CI: 1.066– 1.170, p < 0.001), coronary heart disease (CHD) (OR = 1.004, 95% CI: 1.002– 1.006, p < 0.001), essential hypertension (EH) (OR = 1.009, 95% CI: 1.005– 1.013, p < 0.001) as well as Stroke (OR = 1.003, 95% CI: 1.001– 1.004, p < 0.001). The results of multivariable MR analysis revealed that COPD is not significantly associated with CHD after adjusting for IL-6, LDL, or total cholesterol (p> 0.05). Our findings indicated that BMI, smoking initiation, smoking status, obesity, and FEV1 played a role in the causal effect of COPD on HF, EH, and Stroke.Conclusion: We found positive causal relationships between COPD and HF, EH, and Stroke essentially unaffected by other confounding factors. The causal relationship exhibited between COPD and CHD was influenced by confounding factors. BMI, obesity, initiation of smoking, smoking status, and FEV1 were the mediators between COPD and CVDs.Keywords: chronic obstructive pulmonary disease, cardiovascular diseases, Mendelian randomization

Abstract The current experiments searching for neutrinoless double- $$\beta $$ β ( $$0\nu \beta \beta $$ 0 ν β β ) decay also collect large statistics of Standard Model allowed two-neutrino double- $$\beta $$ β ( $$2\nu \beta \beta $$ 2 ν β β ) decay events. These can be used to search for Beyond Standard Model (BSM) physics via $$2\nu \beta \beta $$ 2 ν β β decay spectral distortions. 100Mo has a natural advantage due to its relatively short half-life, allowing higher $$2\nu \beta \beta $$ 2 ν β β decay statistics at equal exposures compared to the other isotopes. We demonstrate the potential of the dual read-out bolometric technique exploiting a 100Mo exposure of 1.47 kg $$\times $$ × years, acquired in the CUPID-Mo experiment at the Modane underground laboratory (France). We set limits on $$0\nu \beta \beta $$ 0 ν β β decays with the emission of one or more Majorons, on $$2\nu \beta \beta $$ 2 ν β β decay with Lorentz violation, and $$2\nu \beta \beta $$ 2 ν β β decay with a sterile neutrino emission. In this analysis, we investigate the systematic uncertainty induced by modeling the $$2\nu \beta \beta $$ 2 ν β β decay spectral shape parameterized through an improved model, an effect never considered before. This work motivates searches for BSM processes in the upcoming CUPID experiment, which will collect the largest amount of $$2\nu \beta \beta $$ 2 ν β β decay events among the next-generation experiments.

BACKGROUND: Obesity is a progressive health and economic problem of modern society. Endocrine-metabolic changes in childhood and adolescence have an impact on cognitive status, which is accompanied by changes in the perfusion of gray and white matter of the brain, as well as its architectonics.AIM: Verification of fatty acid (FA) levels in relation to cognitive function and brain neuroimaging data in adolescents with obesity.MATERIALS AND METHODS: The study is a single-center observational one-stage comparative. 60 teenagers aged 10–16 years were selected to participate. The main study group was formed of 34 adolescents with obesity (SDS BMI ≥+2.0), 26 subjects with normal weight made up the control group. The calculation of SDS BMI was performed on the WHO «Anthroplus» calculator. To obtain anthropometric data, a vertical height meter MSK — 233, scales and a soft centimeter tape were used. The levels of free fatty acids in the blood serum were determined by chromatomass spectrometry on the Agilent 7000B detector. For neurocognitive testing, the Montreal Cognitive Function Assessment Scale and the Ray test were used. Contrast-free perfusion magnetic resonance imaging of the brain was performed on a Signa Creator “E” MR tomograph by GE Healthcare, 1.5 Tl, China, by Arterial Spin Labeling. For MR tractography, the GE Sygna Creaton 1.5 Tesla apparatus was used using the method of diffusion imaging with high angular resolution HARDI (High Angular Resolution Diffusion Imaging). Statistical processing of the obtained results was carried out using IBM SPSS Statistics software.RESULTS: According to the results of a comparative analysis, adolescents with obesity were distinguished by an increased content of free LC and a decrease in cognitive functions (p

Molecular targets for a promising antitumor agent based on trans-stilbene containing a pyridoxine fragment were identified. The lead compound, (E)-6-(3,4-dimethoxystyryl)-2,2,5,8-tetramethyl-4H-[1,3] dioxino[4,5-c]pyridine, was found to selectively induce apoptosis in MCF-7 breast adenocarcinoma cells overexpressing estrogen receptor, but not in MDA-MB-231 cells negative for estrogen receptor. The mechanism by which the novel trans-stilbene derivative acts as a selective estrogen receptor modulator was analyzed, and the affinity for human estrogen receptor α (ERα) was assessed by fluorescence polarization. Unlike its structural analogs—tamoxifen and raloxifene, the lead compound showed no affinity for ERα and did not form complexes with it. Therefore, it was concluded that the selective action of the pyridoxine-containing derivative of trans-stilbene on estrogen-positive breast cancer cells occurs through an alternative mechanism. The EC50 values for the displacement of the fluorescent ligand from the ERα active site were 22, 120, and 595 nM for estradiol, raloxifene, and tamoxifen, respectively.

Salma Fleifil,1 Lyna Azzouz,1 Gina Yu,1 Corey Powell,2 Nikhil Bommakanti,1 Yannis M Paulus1,3 1Department of Ophthalmology and Visual Sciences, W. K. Kellogg Eye Center University of Michigan Medical School, Ann Arbor, MI, USA; 2Department of Statistics, University of Michigan, Ann Arbor, MI, USA; 3Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USACorrespondence: Yannis M Paulus, Department of Ophthalmology and Visual Sciences, Department of Biomedical Engineering, University of Michigan, Kellogg Eye Center, 1000 Wall Street, Ann Arbor, MI, 48105, USA, Tel +734 764-4182, Fax +734 936-3815, Email ypaulus@med.umich.eduIntroduction: Diabetic retinopathy (DR) is a leading cause of blindness. Retinal imaging is an important tool to monitor the progression of DR. While seven-standard retinal fields are the traditional method for evaluating DR, ultra-widefield (UWF) imaging allows for improved visualization of peripheral areas of nonperfusion (NP) and neovascularization (NV), which could be used as biomarkers to monitor and predict progression of DR.Methods: A retrospective, cross-sectional study was conducted on 651 eyes from 363 patients diagnosed with type 1 or type 2 diabetes who received UWF-FA over 10 years. Fluorescein Angiography (FA) images were segmented, and surface areas of NP and NV were analyzed using multivariate regression to determine if biomarkers of DR and DR severity are associated with increasing areas of NP and NV.Results: Each additional year with a diagnosis of DR was associated with a 10.75 mm2 increase in the total NP (95% CI, 1.94— 19.56; P = 0.02) and 7.87 mm2 increase in NP far-periphery (95% CI, 1.62— 14.13; P = 0.01). A one-unit change in severity as defined by the Early Treatment of Diabetic Retinopathy Study (ETDRS) classification was associated with a 25.75 mm2 increase in total NP (95% CI, 11.16— 40.33; P = 0.001), a 13.15 mm2 increase in mid-periphery NP (95% CI, 6.93— 19.38; P < 0.0001), and a 12.29 mm2 increase in far-periphery NP (95% CI, 3.62— 20.97; P = 0.01).Discussion: Biomarkers identified through UWF imaging such as total and regional areas of NP can be used to monitor and predict the progression of DR. This may provide a quantitative method for prognostication in patients with DR.keywords: diabetic retinopathy, ultra-widefield fluorescein angiography, nonperfusion, neovascularization

Sarcoma is a group of rare malignant tumors characterized by aggressive growth, active metastasis, and rapid recurrence of the disease. Localized in any part of the body, mainly in the limbs. Ewing's sarcoma in the head and neck area is a challenge for a multidisciplinary team of specialists. The tumor has little sensitivity to conservative methods of special treatment and it is necessary to apply a large surgical treatment. To preserve the quality of life of the patient during surgical operations on the organs of the head and neck, reconstructive operations are required. The article presents a clinical case of treatment and rehabilitation of a patient with maxillary sinus sarcoma.

The technique for investigating isomeric states of nuclei with a large values of energy and total momentum created in the neutron capture reaction has been developed. The technique is based on the spectrometry of multiplicity, energy, and time of registration of instantaneous and delayed gamma cascades of isomer occupancy and decay, respectively. The technique was tested in measurements of the isomeric ratio of the 183W level with energy Eiz = 1.285 MeV with total moment Jπiz = 4-, 5- and lifetime Tiz = 8.2 μs in the neutron absorption reaction, which is equal to αiz = (4.7 ± 0.9)⋅10-3. At large values of the efficiency of registration of acts of settlement and decay of the isomer (0.6 - 0.8), the level of the corresponding background of the spectrometer is only 3.5⋅10-4 s-1. At the countable load at the spectrometer input ∼1.5⋅103 s-1, the expected sensitivity of determining the isomeric ratio in measurements with the mono isotope of the sample is αiz ∼(0.3 ± 1.0)⋅10-4.

The purpose of the research is to study the allergenic and immunotoxic properties of pantohematogen obtained from the blood of female Gorno-Altai maral deer as a functional ingredient of specialized products. Allergenic and immunotoxic properties were assessed in 2020–2022 by intragastric administration of pantohematogen to 395 CBA mice weighing 15–18 g, who received the drug at a therapeutic dose of 50 mg/kg and a maximum single dose of 500 mg/kg. The sensitization of the animal body after a course of administration of pantohematogen was determined by deregulation of mast cells. It has been established that when using the maximum single dose, the degranulation reaction does not exceed 0.2. It is necessary to emphasize that no changes in the leukocyte lysis coefficient were recorded in mice receiving different doses of pantohematogen. The effect of pantohematogen on the mass and cellularity of the immunocompetent organs of animals is also shown. It was noted that even the use of a dose (500 mg/kg) 10 times higher than the therapeutic dose does not affect the cellularity of immunocompetent organs. In general, the materials of experimental studies indicate the absence of allergenic and immunotoxic properties of the tested pantohematogen as a functional ingredient in the development of specialized products with specified therapeutic and prophylactic properties.

Jingliang Wu,1,&ast; Qiao Lu,1,2,&ast; Jialin Zhao,3,&ast; Wendi Wu,3 Zhihua Wang,1 Guohua Yu,4 Guixiang Tian,2 Zhiqin Gao,2 Qing Wang5 1School of Medicine, Weifang University of Science and Technology, Weifang, 262700, People’s Republic of China; 2School of Life Science and Technology, Shandong Second Medical University, Weifang, 261053, People’s Republic of China; 3School of Clinical Medicine, Shandong Second Medical University, Weifang, 261053, People’s Republic of China; 4Department of Oncology, Weifang People’s Hospital, Weifang, 261000, People’s Republic of China; 5Department of Stomatology, Weifang People’s Hospital, Weifang, 261000, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Qing Wang, Department of Stomatology, Weifang People’s Hospital, Weifang, 261000, People’s Republic of China, Tel +86 158 6362 6389, Email wfwq8899@163.comPurpose: Breast cancer is a prevalent malignancy among women worldwide, and malignancy is closely linked to the tumor microenvironment (TME). Here, we prepared mixed nano-sized formulations composed of pH-sensitive liposomes (Ber/Ru486@CLPs) and small-sized nano-micelles (Dox@CLGs). These liposomes and nano-micelles were modified by chondroitin sulfate (CS) to selectively target breast cancer cells.Methods: Ber/Ru486@CLPs and Dox@CLGs were prepared by thin-film dispersion and ethanol injection, respectively. To mimic actual TME, the in vitro “condition medium of fibroblasts + MCF-7” cell model and in vivo “ 4T1/NIH-3T3” co-implantation mice model were established to evaluate the anti-tumor effect of drugs.Results: The physicochemical properties showed that Dox@CLGs and Ber/Ru486@CLPs were 28 nm and 100 nm in particle size, respectively. In vitro experiments showed that the mixed formulations significantly improved drug uptake and inhibited cell proliferation and migration. The in vivo anti-tumor studies further confirmed the enhanced anti-tumor capabilities of Dox@CLGs + Ber/Ru486@CLPs, including smaller tumor volumes, weak collagen deposition, and low expression levels of α-SMA and CD31 proteins, leading to a superior anti-tumor effect.Conclusion: In brief, this combination therapy based on Dox@CLGs and Ber/Ru486@CLPs could effectively inhibit tumor development, which provides a promising approach for the treatment of breast cancer.Keywords: drug delivery, cancer-associated fibroblasts, glucocorticoids, combination therapy

Objective. Assessment of renal artery stenosis significance in patients with resistant arterial hypertension using non-invasive diagnostic methods and to compare them with the results of selective angiography and methods of physiological assessment of renal artery stenoses.Materials and methods. Prospectively, 156 patients with drug-resistant arterial hypertension and signs of renal artery stenosis detected by doppler ultrasonography of renal arteries were included in the study. Subsequently, 25 patients were excluded from the study due to multiple variant renal blood supply and bilateral renal artery stenosis. The remaining patients (n=131) underwent selective angiography of renal arteries, and 66 of them additionally underwent CTA of renal arteries with intravenous contrast. If the artery narrowing was 90% or more (n=27) in diameter, the stenosis was considered hemodynamically significant and further stenting of the affected artery was performed, and in case of 60-90% stenosis (n=52) additional assessment of functional significance of the stenosis was performed by measuring translesional pressure gradient, fractional blood flow reserve, instantaneous blood flow reserve (iFR) and Pd/ Pa ratio. Results. Among all patients (n=131), in whom the doppler ultrasonography of renal arteries showed signs of unilateral renal artery stenosis, after angiography combined with additional methods of functional significance assessment, hemodynamically significant renal artery stenosis was confirmed in 41% of cases (n=54). Thus, the sensitivity of doppler ultrasonography of renal arteries in detection of hemodynamically significant stenoses was 74%, prognostic value of positive 78% and negative result 64% (p90% and 52 patients had stenosis 60-90%. Patients with stenosis

Objectives. To evaluate the influence and efficiency of using microwave irradiation on the dichlorocarbenation of polar olefins. To determine the conditions (reaction time and process temperature) under which the maximum yield of target gem-dichlorocyclopropanes is achieved.Methods. The target compounds were obtained by classical methods of organic synthesis— acetalization of polyols and dichlorocarbenation of unsaturated compounds. The preparation of gem-dichlorocyclopropanes was carried out using the microwave activation method on a Sineo device (microwave system for organic synthesis, made in China). In order to determine the qualitative and quantitative composition of the reaction masses, gas–liquid chromatography (using the Kristall 2000 hardware complex), mass-spectroscopy (using Chromatek-Kristall 5000M device with NIST 2012), and nuclear magnetic resonance spectroscopy (using Bruker AM-500 device with operating frequencies of 500 and 125 MHz) were carried out.Results. Under microwave irradiation at 25°C for 2 h with the maximum yield (92–98%), the target substituted gem-dichlorocyclopropanes were obtained: 2-(2,2-dichloro-3-methylcyclopropyl)-1,3-dioxolane, 2-(2, 2-dichloro-3-phenylcyclopropyl)-1,3-dioxolane, 8,8-dichloro4-isopropyl-3,5-dioxabicyclooctane, diethyl-2,2-dichloro-3-phenylcyclopropane-1,1-dicarboxylate, and diethyl-2,2-dichloro-3isopropylcyclopropane-1,1-dicarboxylate.Conclusions. Under the conditions herein proposed, the use of the microwave stimulation method in the dichlorocarbenation of double C=C bonds containing polar substituents allows the reduce the temperature and reaction time to be significantly reduced, and the yield of target gem-dichlorocyclopropanes to be increased.

Methods for assessing post-mastectomy edema: bioimpedance and upper extremity lymphedema index (UEL index) are non-invasive, highly sensitive and easily feasible diagnostic methods, suitable for predicting the prospects of surgical treatment and analyzing the dynamics of edema. The purpose of the study was to study UEL index indicators and bioimpedance data at different stages of upper extremity lymphedema. Material and methods. The study included 64 patients with post-mastectomy edema who underwent bioimpedance, measurement of the circumference of the upper extremity and calculation of the UEL index. Results. The upper extremity lymphedema index and bioimpedance parameters (fluid volume in the limb and the ratio of extracellular fluid to total fluid volume) characterizing the liquid component significantly increased at stages II and III of lymphedema in comparison with the control group. a statistically significant correlation was revealed between the upper limb lymphedema index and bioimpedance parameters (R=0.41) and (R=0.49), respectively. Conclusion. Bioimpedance parameters and values of the upper extremity lymphedema index can be interchangeable. The reliability and high sensitivity of these methods for assessing post-mastectomy edema allows us to obtain quantitative criteria for evaluating the liquid component at different stages of lymphedema.

Likui Fang, Fangming Zhong, Wenfeng Yu, Pengfei Zhu, Guocan Yu Department of Thoracic Surgery, Hangzhou Red Cross Hospital, Hangzhou, 310003, People’s Republic of ChinaCorrespondence: Likui Fang, Department of Thoracic Surgery, Hangzhou Red Cross Hospital, Hangzhou, 310003, People’s Republic of China, Email 21618100@zju.edu.cnBackground: The impact of coagulation indicators on postoperative outcomes of patients with constrictive pericarditis undergoing pericardiectomy has been poorly investigated. This study aimed to assess the prognostic role of preoperative coagulation indicators in these patients.Methods: We retrospectively included 158 patients with constrictive pericarditis undergoing pericardiectomy. The diagnostic values of coagulation indicators for postoperative complications were evaluated by ROC curves. Patients were divided into two groups according to the cutoff value calculated by ROC curve. Postoperative outcomes were compared between the two groups. Logistic regression analysis was performed to identify risk factors of postoperative complications.Results: ROC curve showed that among different coagulation indicators, preoperative D-dimer (DD) level could effectively identify patients with postoperative complications (AUC 0.771, 95% CI 0.696– 0.847, P < 0.001). Patients were divided into the low DD group and the high DD group. The comparison of postoperative outcomes suggested that high preoperative DD level was significantly associated with longer durations of vasoactive agents using (P = 0.018), intubation (P = 0.020), ICU stay (P = 0.008), chest drainage (P=0.004) and hospital stay (P = 0.002). Multivariable analysis showed that high preoperative DD level was the independent risk factor of postoperative complications (OR 6.892, 95% CI 2.604– 18.235, P < 0.001).Conclusion: High preoperative DD level was significantly linked to poor postoperative outcomes and could provide an effective prediction ability for postoperative complications in patients with constrictive pericarditis.Keywords: constrictive pericarditis, D-dimer, postoperative complications, outcomes

Introduction. The multikinase inhibitors have demonstrated high clinical efficacy in treatment of the radioiodine refractory differentiated thyroid cancer. At the same time, the inclusion criteria in the studies has reflected a minimum set of characteristics important for beginning of use of these drugs and an approach that takes into account the tumor progression rate (the volume doubling time) in the absence of clinical manifestations of the disease that have made it possible to individualize the start of therapy. Aim. To describe and evaluate the barriers and drivers of use the “watch and wait” approach by Russian oncologists in treatment of the radioiodine refractory thyroid cancer. Materials and methods. We conducted 35 structured interviews with oncologists observing patients after radioiodine therapy in order to evaluate the “watch and wait” approach. unfortunately, data on use and prevalence of this approach in Russia are limited. The present study involved the use of deductive content analysis as well as analysis of implementation model of clinical guidelines and included investigation of issue of implementation of the “watch and wait” approach to current practice of therapy for radioiodine refractory differentiated thyroid cancer. One of the main tasks was to determine the influence of external factors on the treatment of this category of patients. Conclusion. Barriers and drivers of using “watch and wait” approach in the treatment of differentiated radioiodine refractory thyroid cancer are identified at several levels. for wide use of this approach, it is necessary to involve oncologists taking into account their attitude regarding changes in routine practices, patient expectations and to obtain additional data regarding its long-term effectiveness.

Studies related to waste from the juice production of Chaenomeles fruits in food technologies as a natural food additive (source of organic acids, pectin and phenolic substances, and vitamins) are relevant. The research aims to improve food technologies by implementing the Zero-waste concept, which involves the use of secondary products of fruit processing as a source of valuable biologically active substances in the technologies of various food products. For the research, we used a variety mix of Vitamin, Citrine, and Orange Chaenomeles fruits, Chaenomeles pomace after juice extraction, Chaenomeles pomace processing products (water extracts, powder, mashed potatoes), food products (pickled mushrooms, meat pate, yeast products). Standard methods of analysis were used in the course of experimental studies. The qualitative indicators of raw materials and processed products of Chaenomeles were evaluated by organoleptic and physicochemical parameters. As a result of the research, it was found that the waste generated in the production of juice products from the fruits of the Chaenomeles is characterized by a significant content of organic acids, pectin, and phenolic substances, as well as L-ascorbic acid. The rational conditions for obtaining various secondary products of Chaenomeles processing: extract, powder, and puree were determined and experimentally tested. It was proved that the use of secondary products of Chaenomeles processing (mashed potatoes – 7.5 % by weight of flour, powder – 1.5 % by weight of flour, water extract – 40 % by weight of water) in the recipe composition of yeast flour products would reduce the duration of dough fermentation and increase its biological value. The feasibility of using aqueous extracts of Coleoptera in making pickled mushroom snacks as a substitute for citric acid solution for the pretreatment of mushroom raw materials to inactivate mushroom polyphenol oxidase was confirmed. It was determined that in the technologies of meat pates, the combination of meat raw materials (pork) and 8 % hydrated powder obtained from Chaenomeles pomace provides high organoleptic characteristics of the paste, reduces the content of animal fats and minimizes the caloric content of the paste; positively affects the moisture retention capacity and stability of the minced emulsion; increases the biological value of the product due to plant components.

The article presents the results of a scientific and economic experiment to study the influence of different levels of Bypass soybeans in the diets of high-yielding cows on feed costs per kilogram of milk and reproductive functions of cows. Provision of the rations of the experimental cows with an unequal amount of the difficult-to-dissolve fraction of crude protein, at the expense of Bypass soybeans, affected their consumption of bulk feed. The experimental cows consumed 4.1–4.8 kg from the specified 5 kg of alfalfa hay, 20.8–24.6 kg from 25 kg of corn silage, and 8.4–9.7 kg from 10 kg of alfalfa hay. The consumed feed provided cows with crude protein at the level of 16.0–16.5 % of the dry matter, its poorly soluble fraction at the level of 27.3 % of the crude protein in the 1st control group, 31.1 % in the 2nd experimental group, 35.2 % – in the 3rd experimental group and 40.0 % – in the 4th experimental group. The sugar-starch-protein ratio was normal and ranged from 2.20 to 2.28 : 1 with a norm of 2.1 : 1. The micronutrient rations were balanced due to micronutrients of organic origin. On the basis of the obtained data, it was proved that the lowest feed consumption per 1 kg of milk was observed in cows of the 4th experimental group, which were fed rations with 2 kg of Bypass soybeans, where the level of insoluble protein was 40 % of crude protein and these consumptions were 6.5 MJ against 7.4 MJ in the 1st control group. Cows of the 4th experimental group also had the best insemination and service period indicators. The duration of the service period in the experimental groups was less in percentage compared to the animals of the 1st control group by: 15.0 in the 2nd; 25.6 in the 3rd and 32.9 in the 4th. Partial and complete replacement in the diets of cows of the 2nd, 3rd and 4th experimental groups of soybean meal with Vypass soybeans at the level of 1.0; 1.7 and 2 kg of compound feed concentrate in the first 100 days of lactation has a positive effect on the physiological state, stimulates appetite and provides normal conditions for fertilization and embryo development.

The one-dimensional problem of the linear stability of dynamic states of local thermodynamic equilibria with respect to small perturbations was studied for the case when the Vlasov–Poisson electron gas contains electrons with a stationary distribution function that is constant in physical space and variable in a continuum of velocities. The absolute instability of all considered one-dimensional dynamic states of any local thermodynamic equilibrium was proved using the direct Lyapunov method. The scope of applicability of the Newcomb–Gardner–Rosenbluth sufficient condition for linear stability was outlined. An a priori exponential estimation was obtained for the rise of small one-dimensional perturbations from below. Analytic counterexamples to the spectral Newсomb–Gardner theorem and the Penrose criterion were constructed. Earnshaw’s theorem was extended from classical mechanics tostatistical one.

Abstract The $$K^{+} \rightarrow \pi ^{+}\pi ^{0}\pi ^{0}\gamma $$ K + → π + π 0 π 0 γ decay is observed by the OKA collaboration. About 60 events of the decay observed with signal:noise $$\approx 1$$ ≈ 1 . The branching ratio obtained by normalization to $$K^{+} \rightarrow \pi ^{+}\pi ^{0}\pi ^{0}$$ K + → π + π 0 π 0 is measured to be $$(3.7 \pm 0.9(stat) \pm 0.3(syst))\times 10^{-6}$$ ( 3.7 ± 0.9 ( s t a t ) ± 0.3 ( s y s t ) ) × 10 - 6 for $$E_{\gamma }^*>10\,\textrm{MeV}$$ E γ ∗ > 10 MeV . The branching ratio, $$\gamma $$ γ energy spectrum and angular distribution are consistent with ChPT prediction.

The article examines the development of politicized Shiite Islam in Iran as a type of autopoiesis (N. Luhmann) of this religious tradition. The topic arises from the actualization of the influence of religion on the life of a number of societies that have relied on technological modernization, as well as the importance that the topic of the optimal combination of modernization and traditional values is acquiring today. The purpose of the study is, based on an analysis of the activities of the Shiite clergy, to characterize the uniqueness of the revaluation of the values of Iranian society in the course of the development of modern philosophy and ideology of Shiism. The objectives of the study were: 1) to systematize the existing scientific approaches to the process of politicization of Shiism in the second half of the 20th – early 21st centuries; 2) consider the connection between political processes and the specific theology of Shiism; 3) reveal the dynamics of the relationship between the values of tradition and modernization in the teachings of Shiite religious figures; 4) clarify the specificities of the stages of influence of Imami Islam on the religious and political life of modern Iran. The research was based on theological and scientific writings of Islamic spiritual leaders, their opponents and followers. The key method used was historical-genetic, which made it possible to analyze the formation of Shiism in Iran in the historical and cultural context; and comparative, with the help of which the specifics of the various stages of the autopoietic formation of Iranian Shiism against the background of other denominations of Shiism and Islam in general were clarified. It has been proven that consideration of the path of development of the philosophical and ideological aspects of Shiism theology from the perspective of N. Luhmann's theory makes it possible to trace not only the external, but also the autochthonous foundations of the complex reverses of this religious model of self-awareness of society; as well as identify and characterize the functional specificity and dynamic characteristics of the process of politicization of Shiism in Iran. In modern Iran, theology influences the consciousness of believers, and, as a consequence, social and political processes, and not vice versa, as is commonly believed in relation to modern societies. The role of Imami Islam (Shiites) in the formation of values and behavioral attitudes during the actual structuring of relations in society turns out to be key for Iran. At the same time, however, this theology contains the potential for historical change: the very theological system changes, starting from its own concepts and transforming them. This constructivist approach turns out to be compatible with the emphasis on traditionalism. The logical possibility of such collaboration in building a new type of society is justified within the framework of the development of the theory of legal guardianship.

Abstract A high-statistics data sample of the $$K^{+}$$ K + decays is recorded by the OKA collaboration. A missing mass analysis is performed to search for a light invisible pseudoscalar axion-like particle (ALP) a in the decay $$K^{+} \rightarrow \pi ^{+} \pi ^{0} a$$ K + → π + π 0 a . No signal is observed, and the upper limits for the branching ratio of the decay are calculated. The $$90\%$$ 90 % confidence level upper limit changes from $$2.5\cdot 10^{-6}$$ 2.5 · 10 - 6 to $$2\cdot 10^{-7}$$ 2 · 10 - 7 for the ALP mass from 0 to 200 MeV/ $$c^{2}$$ c 2 , except for the region of $$\pi ^{0}$$ π 0 mass, where the upper limit is $$4.4\cdot 10^{-6}$$ 4.4 · 10 - 6 .

Abstract State-of-the-art physics experiments require high-resolution, low-noise, and low-threshold detectors to achieve competitive scientific results. However, experimental environments invariably introduce sources of noise, such as electrical interference or microphonics. The sources of this environmental noise can often be monitored by adding specially designed “auxiliary devices” (e.g. microphones, accelerometers, seismometers, magnetometers, and antennae). A model can then be constructed to predict the detector noise based on the auxiliary device information, which can then be subtracted from the true detector signal. Here, we present a multivariate noise cancellation algorithm which can be used in a variety of settings to improve the performance of detectors using multiple auxiliary devices. To validate this approach, we apply it to simulated data to remove noise due to electromagnetic interference and microphonic vibrations. We then employ the algorithm to a cryogenic light detector in the laboratory and show an improvement in the detector performance. Finally, we motivate the use of nonlinear terms to better model vibrational contributions to the noise in thermal detectors. We show a further improvement in the performance of a particular channel of the CUORE detector when using the nonlinear algorithm in combination with optimal filtering techniques.

Aim. To present data from the AURA Registry (real-world data registry on AlbUminuRia detection rate among patients with previously undiAgnosed chronic kidney disease). It is important to perform population studies both to study the occurrence of markers (albuminuria (AU), decreased glomerular filtration rate (GFR)) and the prevalence of chronic kidney disease (CKD), which will provide information on the actual detection rate of CKD and the related markers in territories included in the registry of research centers.Material and methods. The article presents the first data from the AURA registry. Recruitment was carried out from March 6, 2023 to January 23, 2024. Thirty-four research centers in various federal districts of the Russian Federation and 104 doctors took part in the recruitment. We included 4580 subjects over the age of 40 years who had no previously established diagnosis of CKD and did not have type 1 or type 2 diabetes. During recruitment, the researchers were guided by the AURA study protocol (Version 1.7/12-26-2022).Results. AU more than 20 mg/g was detected in 64,9% of cases. At the same time, AU is more common at GFR values that may correspond to stage 3A of CKD. The rarer occurrence of AU in those examined with GFR >60 ml/min/1,73 m2 may be explained by less severe renal damage at this CKD stage. The incidence of AU was significantly higher in men, older people, smokers, people with metabolic syndrome, hypertension (HTN), prediabetes and overweight. The occurrence of AU also increased as HTN grade increased. AU detection rate was associated with hypertriglyceridemia, a high blood level of C-reactive protein, which is an integrative marker of inflammation that negatively affects cardiovascular risk.Conclusion. The presented first data from the AURA registry demonstrated the high AU prevalence in people over 40 years of age. A high incidence of AU was typical for patients with HTN, coronary artery disease, atrial fibrillation, heart failure, and prediabetes. An association has been demonstrated between the high incidence of AU and male sex, age, overweight, hyperuricemia, dyslipidemia, and a number of other cardiovascular risk factors.

Objective. Materials and methods: 82 patients with type 2 diabetes mellitus participated, which were divided into 2 groups: probable sarcopenia and comparison groups. Conducted: questionnaire surveys (Health Status Survey (SF-36)), Sarcopenia quality and life assessment (SarQoL)), strength, assistance with walking, getting up from a chair, climbing stairs and falling — Strength, Assisted walking, getting up from a chair, Climbing stairs and Falls (SARC-F)), assessment of walking speed and physical activity, carpal dynamometry, bioimpedancemetry, analysis of laboratory parameters. Results: the difference between the conducted questionnaires is statistically insignificant. According to bioimpedansometry, obesity of the 1st degree was recorded in the lesions. In the group with presarcopenia, the main decrease in body composition parameters decreases. In addition, in the main group, the rate of intake is reduced, and decompensation of carbohydrate and lipid metabolism occurs. Differences were considered significant at p < 0.05. Conclusion. Dynamometry and bioimpedance can be used for primary screening of sarcopenia in patients with type 2 diabetes. Maintaining the main indicators of glycemia, correction of dyslipidemia, compensation for obesity D and hypocalcemia of obesity in muscle mass and mass.

Guocan Yu,1 Yanqin Shen,2 Liwei Yao,2 Xudong Xu1 1Zhejiang Tuberculosis Diagnosis and Treatment Center, Hangzhou Red Cross Hospital, Affiliated Hangzhou Chest Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, People’s Republic of China; 2Department of Nursing, Hangzhou Red Cross Hospital, Affiliated Hangzhou Chest Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, People’s Republic of ChinaCorrespondence: Liwei Yao; Xudong Xu, Email ylwmed@163.com; xuxudong234@163.comPurpose: This study aimed to evaluate the efficacy of nanopore sequencing for diagnosing pulmonary tuberculosis (PTB) using smear-negative clinical specimens.Methods: We conducted a retrospective study based on a review of patient medical records to assess the accuracy of nanopore sequencing as a diagnostic tool for smear-negative PTB. Compared with clinical diagnosis, we determined the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and area under the curve (AUC) of nanopore sequencing.Results: A total of 647 patients were evaluated. Nanopore sequencing demonstrated an overall sensitivity of 91.7%, specificity of 85.3%, PPV of 95.1%, NPV of 76.4%, and AUC of 0.88. Notably, the overall diagnostic accuracy of nanopore sequencing was significantly higher than that of Mycobacterium tuberculosis (MTB) culture technique.Conclusion: Nanopore sequencing exhibited satisfactory overall diagnostic accuracy for smear-negative PTB, regardless of MTB culture status. Therefore, if conditions permit, nanopore sequencing is recommended as a diagnostic method for smear-negative PTB.Keywords: nanopore sequencing, smear-negative specimens, pulmonary tuberculosis, diagnostic accuracy

Aim. To study the potential of bacteria strains P. chlororaphis BZR 245‐F and Pseudomonas sp. BZR 523‐2 to inhibit the growth of Fusarium ear blight pathogens through the example of the fungus F. graminearum and to reduce the accumulation of deoxynivalenol (DON) and zearalenone (ZEN) in vitro. Materials and Methods. Antifungal metabolites of Pseudomonas bacteria were analysed by thin layer chromatography and bioautography. An experiment on the effect of liquid culture and supernatant of bacteria on the growth and toxin production of the fungus F. graminearum in vitro was carried out on wheat grain. Analysis of grain for mycotoxins was carried out by HPLC‐HRMS. Results. The ability of strains P. chlororaphis BZR 245‐F and Pseudomonas sp. BZR 523‐2 to produce antifungal metabolites was found, while inhibiting the growth of the fungus F. graminearum 60318 in vitro. The content of DON decreases both under the action of the liquid culture and supernatant of P. chlororaphis BZR 245‐F bacteria by 60 % and 70 %, respectively, and the liquid culture and supernatant of Pseudomonas sp. BZR 523‐2 bacteria by 75 % and 90 %, respectively. The accumulation of ZEN is also significantly suppressed under the influence of liquid cultures and supernatants of bacterial strains. The amount of ZEN decreases under the influence of liquid culture and supernatant of P. chlororaphis BZR 245‐F bacteria by 80 % and 95 %, respectively. Liquid culture and supernatant of Pseudomonas sp. BZR 523‐2 bacteria inhibited the accumulation of ZEN by 60 % and 84 %, respectively. Conclusion. The results obtained through the study of the effect of liquid cultures and supernatants of P. chlororaphis BZR 245‐F and Pseudomonas sp. BZR 523‐2 strains on the growth and toxin production of the fungus F. graminearum 60318 in vitro allow us to consider these strains as potential producers of effective biofungicides against toxin‐producing fungi. Further studies of these bacterial strains in vitro and in vivo on plants are needed.

Aim. To summarise and analyse scientific data on Cucumber mosaic virus strains (CMV – Cucumber mosaic virus) (Martellivirales: Bromoviridae, Cucumovirus) isolated from ornamental plants in the Far East of the Russian Federation. Discussion. The paper describes the genome structure and tripartite organization of CMV virions. Strains of this virus isolated from ornamental cultures in the south of the Russian Far East are described: Garden balsam, Common snapdragon, Dahlias, Hybrid gladiolus, Hybrid delphinium, Cambria, Indian canna, Cattleya, Tiger lily, Garden petunia, Primula obconica, Moth orchids, Fatshedera from Lize Freres, Weeping fig, Common hollyhock, Purple coneflower. The physicochemical properties (i. e. point of thermal inactivation, period of preservation of infectivity at a temperature of 20 °C, maximum dilution of juice causing disease of healthy plants) and the symptoms of these strains on a wide range of species and varieties of indicator plants are systematised according to the Russian Collection of East Asian Viruses, functioning at the Laboratory of Virology of the Federal Scientific Center for East Asia Terrestrial Biodiversity, Far Eastern Branch, Russian Academy of Sciences. Conclusions. The limiting factor in the development of floriculture is infectious diseases, among which viral infections (including CMV) are of the greatest importance, demonstrating a high strain diversity in the south of the Russian Far East. Thus, the study of the biological, physico‐chemical, immunochemical and molecular biological properties of CMV is an urgent task, as it opens up the possibility of studying isolates of this virus and classifying its strains taking into account individual characteristics and kinship relationships.

Semaglutide is a representative of analogues of the incretin hormone human glucagon-like peptide-1 (GLP-1) and is currently used in Russia for the treatment of type 2 diabetes mellitus (T2DM; in monotherapy and in combination therapy), including patients with obesity and overweight.The aim of the work was to conduct a comparative assessment of the physicochemical properties, a biological activity, bioequivalence and safety, including tolerability and immunogenicity, of the drug Quincent® (semaglutide, 1.34 mg/ml, a solution for a subcutaneous administration, Promomed Rus LLC, Russia) and the drug Ozempic® (semaglutide, 1.34 mg/ml, a solution for a subcutaneous administration, Novo Nordisk A/S, Denmark) when administered to healthy volunteers.Materials and methods. To assess the degree of similarity of the study drug Quincenta® (semaglutide, 1.34 mg/ml, a solution for a subcutaneous administration, Promomed Rus LLC, Russia) with a chemically synthesized active substance to the original (reference) drug Ozempic® (semaglutide, 1.34 mg/ml, a solution for a subcutaneous administration, Novo Nordisk A/S, Denmark), a comparative study of physicochemical properties and a biological activity was carried out. To assess the bioequivalence of the study drug and the reference drug, an open randomized parallel comparative study with the participation of healthy volunteers (n=54), 54 participants of which had been included in the population, was conducted. The volunteers were randomized into 2 groups in a 1:1 ratio, and received a single dose subcutaneously either of the study drug (domestic semaglutide at a dose of 0.5 mg) or the reference drug (foreign semaglutide at a dose of 0.5 mg). The mode of administration was in the morning on an empty stomach. A semaglutide concentration was determined in serum samples using a previously validated enzyme-linked immunosorbent assay (ELISA) method. A quantitative determination of antibodies to semaglutide in the human serum by ELISA was carried out with a microplate photometer using ready-made kits pre-validated by the manufacturer. The conclusion about the bioequivalence of the compared drugs was made using an approach based on the assessment of 90% confidence intervals for the ratios of the geometric mean values of the parameters Cmax, AUC(0–t) of semaglutide in the measurement original units.Results. The results of the comparative analysis of the study drug and the reference drug demonstrate the comparability of their physicochemical properties and biological activity. The results of the clinical study demonstrated the bioequivalence of the test drug and the reference drug. Thus, the pharmacokinetic parameters of the drugs were comparable to each other: the Cmax value for the study drug was 42.088±8.827 ng/ml, for the reference drug Ozempic® it was 42.2556±7.84. Herewith, the half-life for the study drug and the reference drug was 168.39±39.47 and 157.99±28.57 hours, respectively. The resulting 90% confidence intervals for the ratio of the Cmax and AUC0–t values of the study drug and the reference drug were 90.89–109.15 and 91.66–111.27%, respectively. The tolerability of the drugs in the volunteers was notified as good. No adverse events were recorded during the study. No serious adverse events were reported throughout the study. According to the results of the immunogenicity analysis, no antibodies to Russian-made semaglutide were detected in the blood serum of the volunteers, which indicated the lack of Results. The results of a comparative analysis of the study drug and the reference drug demonstrate the comparability of physicochemical properties and biological activity. The results of the clinical study demonstrated the bioequivalence of the study drug and the reference drug. Thus, the pharmacokinetic parameters of the drugs were comparable to each other: the Cmax value for the study drug was 42.088±8.827 ng/ml, for the reference drug Ozempic® this figure was 42.2556±7.84. At the same time, the half-life for the study drug and the reference drug was 168.39±39.47 and 157.99±28.57 hours, respectively. The resulting 90% confidence intervals for the ratio of the Cmax and AUC0–t values of the study drug and the reference drug were 90.89–109.15 and 91.66–111.27%, respectively. Tolerability of the drugs in volunteers was noted as good. No adverse events were recorded during the study. No serious adverse events were reported throughout the study. According to the results of the immunogenicity analysis, no antibodies to Russian-made semaglutide were detected in the blood serum of the volunteers, which indicated the lack of the drug immunogenicity.Conclusion. In the course of the study, the comparability of the physicochemical properties and biological activity of the studied Russian drug with the chemically synthesized active substance Quincenta® to the reference drug Ozempic® was confirmed: the activity range of the studied drugs was within 80–120% in relation to the standard sample of semaglutide. The bioequivalence and a similar safety profile, including the immunogenicity and tolerability of the Russian drug Quincenta® (semaglutide 1.34 mg/ml, Promomed Rus LLC, Russia) were shown in comparison with the foreign drug Ozempic® (semaglutide 1.34 mg/ml, Novo Nordisk A/C, Denmark).

The isotopic composition (δ18O values) of snow layers, constructing snow cover to the time of reaching maximum snow water equivalent (SWE), was compared with the isotopic content of snow precipitated over the whole the winter season 2018/19 on the territory of the Meteorological Observatory of the Lomonosov Moscow State University (Moscow, Russia). Snow-sampling was carried out in a trench 20 m long simultaneously with detailed measurements of spatial variability of the structural characteristics of snow depth. Sampling was conducted for each precipitation event over the winter season, with the amount of precipitation also documented. It was found that the spatially-distributed enrichment with heavy oxygen isotopes along the trench fell within the range of 0–3.5‰, with average values for the four main formed snow layers changing from 1.3 to 2.5‰. The enrichment was not much dependent on the age of snow layer in the snowpack, and it was even more pronounced in the upper layers. This suggests that the post-precipitated change in the isotopic composition of snow cover for the conditions of the investigated site mainly took place when the snow was exposed to the atmosphere (due to sublimation and evaporation), while the processes of dry and wet metamorphism were either less important or even led to leveling the effects of isotopic fractionation. A positive correlation was found between the isotope composition of snow and the spatially varying snow density in each layer. This is most probably related to involvement of wind influence into the snow accumulation resulting in more dense snow. The spatial variability of the isotope composition of snow in each layer was smaller than changes in snow density and snow water equivalent.

This study is devoted to the problem of the relationship between judicial precedent and the analogy of law. The author, studying the nature of judicial law-making, notes that law-making activity is inherent in any body of the state, if the constitution does not directly prohibit it from creating new norms. The article provides a critique of the approach to understanding the analogy of law as a way of applying law. The author notes that it is impossible to apply abstract principles directly, the court, like a legislator, must first formulate a specific provision (rule of law) on the basis of them, and then apply it. Hence it is concluded that the analogy of law is a form of judicial precedent. Normative substantiation of the possibility of Russian courts to create law requires an answer to the question about the characteristics of the judicial norm and its place in the system of sources of modern Russian law. The author comes to the conclusion that the judicial norm is causal, special and retroactive, and it can take any place, with the exception of the provisions of the Constitution of the Russian Federation, which can only be changed through the direct will of the people (chapters 1, 2, 9). The article also proposes to supplement the institution of analogy of law with norms on the mandatory request of the court considering the case to the Presidium of the Supreme Court of the Russian Federation on the creation of a judicial norm.

This article analyzes the inaugural speech (discours de réception) delivered in 1981 by Marguerite Yourcenar, the first female writer elected as a member of the French Academy. Since the 17th century, such speeches have been mandatory for newly elected academicians, serving as a communicative product of academic speech practice. The study notes that commemorative speeches in honor of deceased members of the academy typically involve the use of praise as an evaluative strategy. Following the genre’s requirements, Marguerite Yourcenar addresses the conceptual space of “the value of literary heritage” of Roger Caillois, whose “academic chair” she was about to occupy as a new academician. In his work, she identifies authorial conceptual metaphors (such as “life is a game”: vie — jeu) that align with her own value system, confirming this alignment with examples from her own books or life experience. The article highlights authorial conceptual oppositions, including men — women (homes / femmes), friendship — hatred (amitié / haine), and life — death (vie / mort). As a newly elected academician, Marguerite Yourcenar perceives the French Academy as a scientific institution with both merits (Académie — science, littérature, connaissance) and shortcomings (Académie — préjugés, archaїsme), reflecting her personal position as well as her gender and social identity.

Representation of the Stalingrad Battle in the British Press is examined in this study, with a focus on the coverage of events on the Volga in the regional British press. The specific portrayal of the Stalingrad Battle in the Western Post newspaper is analyzed, particularly during the defensive battles and the Soviet counteroffensive known as “Operation Uranus.” A review of all issues of the newspaper from 1942-1943 dedicated to the Stalingrad Battle is conducted. The novelty of this research lies in the introduction of a new historical source that is virtually unknown to both Russian and global readers. Typically, historiography examines materials from the central press. The relevance of this study is justified by the need to reveal a new perspective in the examination of the British press during wartime. The authors focus on how the Western Post portrayed the courage of Soviet soldiers, the professionalism of command, and the heroism of civilians in Stalingrad. It is proven that the local British press during World War II provided an overall objective picture of the battle on the Volga but paid less attention to events on the Eastern Front compared to operations in North Africa and the Mediterranean.

Likui Fang, Wenfeng Yu, Guocan Yu, Gang Chen, Bo Ye Department of Thoracic Surgery, Hangzhou Red Cross Hospital, Hangzhou, 310003, People’s Republic of ChinaCorrespondence: Likui Fang, Department of Thoracic Surgery, Hangzhou Red Cross Hospital, Hangzhou, 310003, People’s Republic of China, Email 21618100@zju.edu.cnBackground: Tuberculous constrictive pericarditis (TCP) is recommended to be treated with anti-tuberculosis (TB) therapy before pericardiectomy. Whether different preoperative anti-TB regimens may lead to different outcomes is unclear.Methods: We retrospectively collected patients diagnosed as TCP and received pericardiectomy from April 2016 to June 2023. The study patients were assigned into the active TCP (A-TCP) group and the inactive TCP (IA-TCP) group according to the results of Mycobacterium tuberculosis (MTB) culture and MTB RNA assay. Baseline characteristics including anti-TB regimens and surgical outcomes were compared between the two groups. Logistic regression analysis and subgroup analysis were conducted to identify the protective factors of A-TCP.Results: Of the 102 study patients, 24 was in the A-TCP group and 78 was in the IA-TCP group. The rate of preoperative anti-TB regimen containing pyrazinamide was 37.5% in the A-TCP group, as compared with 74.4% in the IA-TCP group (P = 0.001). Multivariate analysis showed that preoperative use of pyrazinamide was the protective factor of A-TCP (OR 0.194, 95% CI 0.053– 0.703, P = 0.013). Subgroup analysis based on age also showed consistent findings. In the analyses of surgical outcomes, A-TCP was the independent risk factor of postoperative cardiac complications (OR 4.231, 95% CI 1.317– 13.593, P = 0.015) and associated with longer hospital stay (P = 0.004) and higher hospitalization cost (P = 0.001).Conclusion: A strategy involving anti-TB regimen containing pyrazinamide before pericardiectomy was superior to that without pyrazinamide in the patients with TCP. The strategy was associated with lower risk of A-TCP and might lead to better postoperative recovery and cost-effectiveness.Keywords: tuberculous constrictive pericarditis, pericardiectomy, pyrazinamide, outcomes

Within the project of developing an automatic feed wagon, the initial task is to determine its overall parameters, which directly depend on the wagon volume. (Research purpose) To simulate the automatic feed wagon operation in order to determine the optimal volume ensuring the versatility of its use in cattle cowsheds accommodating different numbers of animals, and practicing different feeding frequency. (Materials and methods) The solution to the problem is based on the calculation of the feeding time taken by the automatic feed wagon. The time must not exceed the limit values established by zootechnical standards. To calculate the feeding time taken by an automatic feed wagon, a graph-theory-based simulation method was used, taking into account the probabilistic nature of the components of feed mixture distribution time balance. The calculations were performed in MS Excel. (Results and discussion) The paper describes the conditions for simulation and considers two options for the automatic feed wagon operation, in particular the option when one robot serves two cowsheds and the other when each cowshed is served by a separate feed wagon. (Conclusions) The second option with a separate automated feed wagon for each cowshed is proved to be more suitable. It has been obtained that in this case, the reduced movement length results in a 27.9-36.2 percent increase in productivity of the automatic feed wagon; besides there is no need to install automatic gates and build an insulated vestibule to move the robot between the cowsheds. Thus, the programming task becomes simplified. It has been found out that an automatic feed wagon having the volume of 2 cubic meters is enough to feed animals in the cowsheds sized 111.9 meters by 26.6 meters and smaller, accommodating up to 340 cows, and with a feeding frequency of 4-8 times a day.

Introduction. The paper describes a fundamentally new method of passive damping of vibrations of a structure in the vicinity of its resonant frequencies. The method is based on the use of piezoeffect. For this purpose, piezoelectric elements are added to the structure, which serve as energy converters (mechanical energy into electrical energy and vice versa).Materials and methods. Piezoelectric elements are polarized piezoceramics with electrodes. Two different types of electrical conditions on the electrodes are used for passive vibration damping: a) the electrodes are short-circuited and b) disconnected electrodes. By changing the electrical conditions on the electrodes, we change the boundary value problem. The spectrum of natural frequencies of the boundary value problem for a structure with short-circuited electrodes differs from the spectrum of natural frequencies of the boundary value problem for the same structure with disconnected electrodes. The idea of the method is as follows: let the vibration frequency of the structure with short-circuited electrodes approach its resonant frequency. Let us disconnect the electrodes, thereby changing the spectrum of natural frequencies of the structure. The vibration frequency, which is the resonant frequency for the structure with short-circuited electrodes, will not be resonant for the structure with disconnected electrodes. As a result of changes in electrical conditions, the amplitudes of the required quantities (deflection, bending moment, shearing force, etc.) will decrease significantly. To evaluate the effectiveness of passive vibration damping, a simple formula is proposed.Results. For a beam undergoing forced bending vibrations in the vicinity of its resonant frequency, calculations were performed using the proposed method of passive vibration damping, tables of vibration damping efficiency in the vicinity of resonant frequencies were obtained, and graphs are plotted. The possibility of increasing the efficiency of vibration damping by choosing the direction of pre-polarization of the piezoelectric material, the location of the electrodes and their number was investigated.Conclusions. The results of studies of passive vibration damping near resonant frequencies using the piezoeeffect confirmed the simplicity and reliability of the proposed method.

A computer model of the system for generating neutron fluxes at the output of a linear electron accelerator has been developed in the Geant4 and PhysList QGSP BIC HP programming environment. With the help of the model, a number of virtual experiments on 107 primary neutrons were carried out. The dependence of the ratio of the number of neutrons, incident on the detector with a reflector to the number of neutrons without a reflector on the radius of curvature of the hemispherical dome of the reflector is determined, which makes it possible to establish the optimal dimensions of the working area. The energy spectra of neutrons incident on the detector are determined. It has been established that when using a graphite reflector 30 × 30 × 30 cm in size with a radius of curvature of the hemispherical dome of 5 cm, the number of neutrons at the location of the detector increases by 16.9 %, and the neutron background in the accelerator bunker decreases by 2.5 times, which is in good agreement with the real experiment made. Such a decrease in the radiation background, according to sanitary standards, will make it possible to increase the accelerator current by 2.5 times and increase the neutron flux.

Xudong Zhu,1,2 Jiahui Yu,3 Fulu Ai,1 Yue Wang,1 Wu Lv,1 Guilin Yu,1 Xiankui Cao,1 Jie Lin1 1Department of General Surgery, Cancer Hospital of China Medical University, Cancer Hospital of Dalian University of Technology, Liaoning Cancer Hospital and Institute, Shenyang, Liaoning, 110042, People’s Republic of China; 2Department of Oncology, Shengjing Hospital of China Medical University, Shenyang, Liaoning, 110004, People’s Republic of China; 3Department of Ultrasound, Shengjing Hospital of China Medical University, Shenyang, Liaoning, 110004, People’s Republic of ChinaCorrespondence: Jie Lin; Xudong Zhu, Department of General Surgery, Cancer Hospital of China Medical University, Cancer Hospital of Dalian University of Technology, Liaoning Cancer Hospital and Institute, Shenyang, Liaoning, 110042, People’s Republic of China, Tel +86 13940081800 ; +86 13354204706, Email linxiran0221@126.com; xdzhu@cmu.edu.cnPurpose: CD24 mediates a “don’t eat me” signal to escape the immune environment. However, the correlation between CD24 and PD-L1 is unclear. This study aimed to assess if CD24 can serve as a target for immunotherapy of triple-negative breast cancer (TNBC).Methods: Data on CD24 expression in breast cancer were acquired using the Oncomine and UALCAN tools. The role of CD24 expression on the prognosis of patients with TNBC was assessed using Kaplan–Meier analyses. Subsequently, STRING and TISIDB databases were used to construct protein–protein interaction networks and to explore immune-related molecules regulated by CD24. Immunofluorescence and immunohistochemistry assays were conducted to validate CD24 and PD-L1 expression and tumor infiltration lymphocyte (TIL) level. Survival analysis was also performed to explore the effect of CD24 and PD-L1 expression and TIL level in patients with TNBC. ShRNA was also used to explore the regulation role of CD24 on PD-L1 expression.Results: CD24 expression was significantly higher in breast cancer than in normal tissues, with high expression being significantly associated with a worse prognosis. CD24 was found to be significantly regulated by chemokines, immunoinhibitors, immunostimulators and TILs. Furthermore, CD24 expression showed a significant positive correlation with PD-L1 expression and a negative correlation with TIL level. In association with PD-L1, CD24 was found to positively regulate lymphocyte costimulation, T cell costimulation, and leukocyte activation. Furthermore, CD24 and PD-L1 co-expression contributed to worse survival outcomes. In addition, CD24 expression was found to attenuate the positive effects of high-level TILs on the prognosis of patients with TNBC. CD24 can also regulate the expression of PD-L1 in TNBC cells.Conclusion: CD24 may attenuate the positive effects of high TIL levels on survival and may facilitate the immune escape of TNBC by regulating PD-L1 expression. Thus, it is a potential target for immunotherapy in TNBC.Keywords: triple negative breast cancer, CD24, PD-L1, prognosis, immunotherapy

Jian Liu,1,&ast; Yuhua Ma,2,&ast; Geping Yu,3 Weiming Wang1 1Department of Nephrology, Ruijin Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, People’s Republic of China; 2Department of Nephrology, Traditional Chinese Medicine Hospital of Kunshan, Jiangsu, People’s Republic of China; 3Department of Nephrology, Tonglu First People’s Hospital, Hangzhou, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Weiming Wang, Department of Nephrology, RuiJin Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, People’s Republic of China, Tel/Fax +86 021-64370045 Ext 665232, Email wwm11120@rjh.com.cnAim: To investigate the association between longitudinal changing patterns of fasting plasma glucose (FPG), and kidney function change in the general population.Methods: We conducted a retrospective, longitudinal cohort study of a health examination center database in China. Subjects who had at least three visits from 2011 to 2018 with baseline eGFR ≥ 60 mL/min/1.73 m2 were enrolled. The FPG trajectories were identified by group-based trajectory modeling (GBTM). We examined the association of eGFR slopes and FPG trajectories by Cox analysis.Results: Totally, 8114 participants were identified. Three heterogeneous FBP trajectories were detected by GBTM as low-stable group (n=7294), moderate-stable group (n=657) and high-stable group (n=163). The high-stable group had lower baseline eGFR, a higher percentage of fast eGFR slope, lower HDL-c, higher LDL-c, higher cholesterol, and higher Lg(triglyceride). Cox analysis showed that the high-stable trajectory was a risk factor for fast eGFR decline (for eGFR slope

Aim. To analyze the use in clinical practice of intravascular coronary assessment methods based on two-year data from the Russian Registry on the Use of Intravascular Imaging and Physiology.Material and methods. Since 2021, the Russian Registry on the Use of Intravascular Imaging and Physiology has included data from 7967 studies in 3932 patients, of which 3086 (38,7%) were studies of intravascular physiology. In 2021, 13 branches from 9 cities participated in the registry, in 2022 — 20 branches from 11 cities. For chronic coronary artery disease, 2484 (80,5%) studies were performed, for acute coronary syndrome (ACS) — 598 (19,4%).Results. In 2022, the registry included 1,9 times more intravascular functional studies compared to 2021. The contribution of different clinics was uneven — 75,7% of studies were performed in three departments. The use of non-hyperemic indexes prevailed over hyperemic ones (58%), and in ACS they were used three times more often. All coronary arteries were examined, most often the anterior descending artery. As a primary diagnosis, 1968 (63,8%) studies were performed, while for the purpose of dynamic control — 122 (3,9%), at the surgical stage — 996 (32,3%). In 85,5% (n=2638) of observations, the functional assessment of stenosis hemodynamic significance directly influenced the treatment tactics.Conclusion. The registry data indicate the significant role of functional methods among intravascular diagnostic options in the practice of domestic clinics participating in the registry. The registry demonstrated their use as a primary diagnosis 2 times more often than for coronary artery stenting. Some indications for the active use of intravascular physiology methods (in acute coronary syndrome, left coronary artery involvement) are currently controversial and require further research.

Aim. To determine the reproducibility of the results of instantaneous wavefree ratio (iFR) and angiography coregistration in patients with coronary heart disease (CAD) with multilevel and diffuse lesions of the coronary arteries.Materials and methods. The study included 34 patients with chronic ischemic heart disease (CAD) with diffuse and/or multilevel coronary stenoses >50%. In 41 coronary arteries, two consecutive iFR pullback of the conductor were performed and paired iFR angio-coregistrations were formed, reflecting changes in iFR gradients (Δ iFR) along the entire length of the studied artery. To compare the results of paired studies, the following values were used: 1) indices of distal iFR; 2) maximum Δ iFR; 3) Δ iFR of all stenoses ˃50%.Results. Comparable values of distal iFR were obtained 0,84 [0,78; 0,89] vs. 0,85 [0,77; 0,88]) with an average difference of the first and second measurements of 0,001, maximum ΔiFR (0,08 [0,05; 0,14] vs. 0,08 [0,05; 0,13]) with a mean difference of -0,004 and ΔiFR of all stenoses >50% (0,05 [0,02; 0,11] vs. 0,05 [ 0,02; 0,10]) with an average difference of -0.001. Lin’s concordance correlation coefficient and Intraclass Correlation Coefficients calculated for the three parameters studied showed a good level of agreement (0,97; 0,98; 0,98) with excellent reliability (0,97; 0,98; 0,97).Conclusion. In patients with multilevel and diffuse lesions of the coronary artery, iFR angio-coregistration has a fairly high reproducibility.

The current paper has presented the study results for the period of 2020–2022 to determine the effect of injury on the quality indicators of seed material in primary seed production of winter wheat varieties (‘Stanichnaya’, ‘Etyud’, Asket, ‘Lidiya’ and ‘Ambar’) during harvesting and post-harvest processing of seeds. There has been established that at different stages of technology in agricultural machines and grain cleaning equipment, during grain production, there was damage to the seed material. There have been identified certain types of injuries that most significantly worsen the sowing quality of seeds and reduce winter wheat productivity. The purpose of the study was to estimate the sowing seed qualities of winter bread wheat varieties of different maturity groups developed by the FSBSI «ARC «Donskoy» depending on their injury by the combine harvester and grain cleaning machines in the primary stages of seed production. The estimation of the quality of winter wheat seed material was carried out on the material obtained during winter wheat harvesting, such as manual threshing (control), threshing with the combine ‘Wintersteiger Classic’, after seed cleaning and sorting equipment VIM-1 «Selection» and Petkus K-531 «Gigant». When harvesting with the combine ‘Wintersteiger Classic’, two threshing modes were used: 1) recommended – 1500 rpm-1; 2) with a reduced speed of the threshing drum – 1000 rpm-1. There has been found that the highest level of seed injury was observed when threshing with a grain harvester (33–54 %). There has been determined that, depending on the type of the trial, the germination energy indicators varied from 89 to 98 %, and laboratory germination from 94 to 99 %. Caryopsis with damage in the embryo area had a high germination rate in laboratory conditions. With an increase in microtraumas, field germination has decreased by 4–17 % compared to the control. The maximum injury and reduction in field germination of seeds was observed in the varieties of the early maturing group. Post-harvest processing of seeds has led to an increase in the percentage of their injury by 1–6 %, regardless of the type of equipment used.

Despite adequate therapy, interstitial lung diseases (ILD) can cause progressive scarring of lung tissue. This type of ILD is known as progressive fibrosing ILD (PF­-ILD). The challenge in diagnosing PF-­ILD lies in the lack of uniformly accepted criteria for a progressive fibrosing phenotype. Most authors use criteria based on clinical features and assessment of functional imaging and radiological findings over time. However, forced vital capacity (FVC) measurement is limited by its variability, and the follow­up lasts 1­2 years. The above diagnostic challenges prevent from prescribing early adequate therapy in patients with progressive ILD, indicting the need to search for new biomarkers of the progressive fibrosing phenotype. We review the most studied and informative biomarkers of fibrosis progression in patients with ILD.

Objectives. To synthesize derivatives of 5-acetyl- and 5-hydroxyalkyl-1,3-dioxanes and evaluate their effect on platelet aggregation and plasma hemostasis.Methods. To determine the qualitative and quantitative composition of the reaction masses, gas chromatography-, chromate mass spectrometry-, and 1H and 13C nuclear magnetic resonance spectrometry methods were used.Results. Derivatives of 5-acetyl- and 5-hydroxyalkyl-1,3-dioxanes were obtained under thermal heating conditions in order to evaluate their effect on platelet aggregation and plasma hemostasis.Conclusions. Derivatives of 5-acetyl- and 5-hydroxyalkyl-1,3-dioxanes were synthesized in high yields. Their effect on platelet aggregation and plasma hemostasis was established.

The combined use of drugs with different mechanisms of action is the main principle of musculoskeletal pain control in rheumatic diseases. However, there are few studies evaluating the efficacy of this approach in real practice.Objective: to determine the efficacy and safety of the combined use of celecoxib, diacerein, and the combination of glucosamine + chondroitin in osteoarthritis (OA) and chronic nonspecific low back pain (NSLBP). Material and methods. Statistical analysis of data obtained during a 3-month open observational study was performed. We included 1569 patients (63.6 % women and 36.4 % men, mean age 58.7 ± 11.0 years) with knee OA (kOA), hip OA (hOA), generalized OA (gOA), and chronic NSLBP with moderate/severe pain (≥ 4 on a numeric rating scale, NRS 0–10) who required nonsteroidal anti-inflammatory drugs. Celecoxib 200 mg twice daily was prescribed, with the dose reduced to 200 mg per day or taken “as needed" after significant pain relief; diacerein 50 mg twice daily; and a medication of glucosamine 250 mg and chondroitin 200 mg, 2 capsules 2–3 times daily. Outcomes were assessed after 3 months using the dynamics of pain, fatigue, dysfunction (according to NRS), and the “Patient Acceptable Symptom State” (PASS) indicator. Results and discussion. 80.2 % of patients completed the 3 month course of treatment, 4.4 % discontinued treatment due to adverse events (AEs), and for 15.4 % of patients there was no follow-up. After 3 months of treatment ≥ 50 % decrease (from baseline) in the severity of symptoms was noted in 83.4 % of patients for pain on movement, in 83.7 % for pain at rest, in 78.6 % for pain at night, in 80.8 % for dysfunction, and in 83.4 % for fatigue. 87.7 % of patients reported PASS. There were no significant differences in treatment outcomes for different localizations of OA and NSLBP: a ≥ 50 % pain reduction in kOA was achieved in 81.6 % of patients, in hOA – in 82.2 %, in gOA – in 85.0 %, in NSLBP – in 88.1 %. AEs were registered in 350 (22.4 %) patients, the most frequent was dyspepsia (n = 280, 17.8 %), diarrhea was recorded in 37 (2.4 %) cases. No serious AEs requiring hospitalization were registered. Conclusion. Combination therapy with celecoxib, diacerein, and a combination of glucosamine and chondroitin significantly reduces the severity of symptoms of OA and NSLBS.

The problem of placer diamond potential on the territory of the western slope of the Urals is considered. The mineral composition of the heavy fraction of the Takata intermediate diamond collector in the Vishera diamondiferous region, which feeds the diamondiferous deposits of the spatially associated Cenozoic depressions, has been studied. Based on known deposits, a brief history of mineralogical studies in the Vishera and Chikman diamond-bearing regions is given. The geological structure, material composition of the detrital material, diamond content, and the proposed sources rocks for the discovered in 2022 currently largest Syuzevskaya placer in the Urals in the Chikman diamondiferous region, are described. Criteria for searching of similar placers in the diamond-bearing regions of the Urals and in the regions with a similar geological structure are proposed. The prospects and problems of the mineralogical method in the Urals region are outlined.