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Antibodies are fundamental effectors of humoral immunity, and have become a highly successful class of therapeutics. There is increasing evidence that antibodies utilize transient homotypic interactions to enhance function, and elucidation of such interactions can provide insights into their biology and new opportunities for their optimization as drugs. Yet the transitory nature of weak interactions makes them difficult to investigate. Capitalizing on their rich structural data and high conservation, we have characterized all the ways that antibody fragment antigen-binding (Fab) regions interact crystallographically. This approach led to the discovery of previously unrealized interfaces between antibodies. While diverse interactions exist, β-sheet dimers and variable-constant elbow dimers are recurrent motifs. Disulfide engineering enabled interactions to be trapped and investigated structurally and functionally, providing experimental validation of the interfaces and illustrating their potential for optimization. This work provides first insight into previously undiscovered oligomeric interactions between antibodies, and enables new opportunities for their biotherapeutic optimization.

The typhoon disaster chain is one of the leading climate risks in constructing the Guangdong–Hong Kong–Macau Greater Bay Area (GBA). In this study, the risks of the typhoon disaster chains including typhoon-induced gales, rainstorms, and storm surges in the GBA, as well as the comprehensive risk of typhoon disaster, are investigated at county level by comprehensively analyzing the hazard, exposure, and vulnerability. The results show that the high- and very-high-risk areas of typhoon–gale disaster chain are located in Zhuhai, Zhongshan, Foshan, Dongguan, central-southern Jiangmen, southern Shenzhen, and parts of Huizhou. The high- and very high-risk areas of typhoon–rainstorm disaster chain include Zhuhai, Zhongshan, Shenzhen, central-southern Foshan, northern Dongguan, central Jiangmen, and central Huizhou. Regarding the typhoon–storm surge disaster chain, the areas at high and very high risk are located in Zhuhai, eastern Zhongshan, and the coastal areas of the Pearl River Estuary. In addition, the comprehensive risk of typhoon disaster is very high in Zhuhai and high in Zhongshan, Jiangmen, Dongguan, and Shenzhen. By verifying the spatial correlation between typhoon disaster risk indexes and actual losses, it is found that the comprehensive risk index of typhoon disaster constructed in this study can better reflect the actual losses. Overall, the findings of this study can provide a scientific basis for typhoon disaster prevention and mitigation in the GBA, and it can also serve as a reference for typhoon disaster risk research in other areas.

Neutrophil extracellular traps (NETs), the product of NETosis, is found to localize pathogens and crystals in immune response. Recent studies have found that excessive NETs lead to disease conditions such as diabetes and its complications like diabetic retinopathy (DR). However, the correlation between NETs and high glucose or DR remains unclear. Here, we found NETs level was significantly increased in the serum of diabetic patients, especially in proliferation diabetic retinopathy (PDR) patients. High glucose dramatically increased NETs production in diabetic individuals with time prolonging. The activation of NADPH oxidase was involved in the NETs process which is triggered by high glucose. Moreover, we verified the infiltration of neutrophils in the eyes and adhesion to vascular endothelial cells in diabetic rat models. NETs formation was observed in the vitreous bodies and retinas of diabetic individuals, which indicates NETs may play a role in the pathogenesis of diabetic retinopathy. Furthermore, anti-VEGF therapy downregulates NETs production indicating that NADPH oxidase-derived ROS may be another signaling pathway involved in anti-VEGF therapy.

Afforestation activities in the Kubuqi Desert, Inner Mongolia, China, have substantially increased tree and shrub coverage in this region. In this study, the response of the surface temperature to afforestation is simulated with the Weather Research and Forecasting model. The surface temperature changes are decomposed into contributions from the intrinsic surface biophysical effect and atmospheric feedback, using the theory of intrinsic biophysical mechanism. The effect of afforestation on the surface temperature is 1.34 K, −0.48 K, 2.09 K and 0.22 K for the summer daytime, the summer nighttime, the winter daytime and the winter nighttime, respectively, for the grid cells that have experienced conversion from bare soil to shrub. The corresponding domain mean values are 0.15 K, −0.2 K, 0.67 K, and 0.06 K. The seasonal variation of surface temperature change is mainly caused by changes in roughness and Bowen ratio. In the daytime, the surface temperature changes are dominated by the biophysical effect, with albedo change being the main biophysical factor. In the nighttime, the biophysical effect (mainly associated with roughness change) and the atmospheric feedback (mainly associated with change in the background air temperature) contribute similar amounts to the surface temperature changes. We conclude that the atmospheric feedback can amplify the influence of the surface biophysical effect, especially in the nighttime.

PlyC, a bacteriophage-encoded endolysin, lyses Streptococcus pyogenes (Spy) on contact. Here, we demonstrate that PlyC is a potent agent for controlling intracellular Spy that often underlies refractory infections. We show that the PlyC holoenzyme, mediated by its PlyCB subunit, crosses epithelial cell membranes and clears intracellular Spy in a dose-dependent manner. Quantitative studies using model membranes establish that PlyCB interacts strongly with phosphatidylserine (PS), whereas its interaction with other lipids is weak, suggesting specificity for PS as its cellular receptor. Neutron reflection further substantiates that PlyC penetrates bilayers above a PS threshold concentration. Crystallography and docking studies identify key residues that mediate PlyCB–PS interactions, which are validated by site-directed mutagenesis. This is the first report that a native endolysin can traverse epithelial membranes, thus substantiating the potential of PlyC as an antimicrobial for Spy in the extracellular and intracellular milieu and as a scaffold for engineering other functionalities.