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ObjectiveTo investigate the genetic information of 57 autosomal InDel loci （A-InDels） included in AGCU InDel 60 fluorescence detection kit in the Beichuan Qiang population of Sichuan Province and evaluate its application value in forensic medicine.MethodsA total of 200 unrelated healthy individuals from Beichuan Qiang population of Sichuan Province were typing detected by AGCU InDel 60 fluorescence detection kit. Allele frequencies and population genetic parameters of the 57 A-InDels were statistically analyzed and compared with the available data of 26 populations.ResultsAfter Bonferroni correction, there was no linkage disequilibrium between the 57 A-InDels, and all loci were in Hardy-Weinberg equilibrium. Except for rs66595817 and rs72085595, the minor allele frequencies of 55 A-InDels were above 0.3. PIC ranged from 0.298 3 to 0.375 0, CDP was 1-2.974 8×10-24, CPEduo was 0.999 062 660, and CPEtrio was 0.999 999 999. The calculation of the genetic distance showed that Beichuan Qiang population had the closest genetic distances with Beijing Han and South China Han populations, but far away from African populations.ConclusionThe 57 A-InDels in AGCU InDel 60 fluorescence detection kit have a good genetic polymorphism in Beichuan Qiang population of Sichuan Province, which can be used as effective supplemental for individual identification and paternity identification in forensic medicine.

Available alumina and active silicon in gibbsite bauxite are important indicators for the quality of bauxite. These two components are commonly determined by Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES). In this method, available alumina in solution after digestion is determined first and active silicon is then determined after acid dissolution of the residue. However, because the solution is not easy to determine, and the residue cannot be digested completely, the results have poor reproducibility. By heating and acidifying the solution and taking Co as the internal standard, the conventional method can be improved upon. Active alumina and active silicon were simultaneously determined by Inductively Coupled Plasma-Mass Spectrometry (ICP-MS), and available alumina was quantified indirectly by calculation. The method precision (RSD) is greater than 3% (n=13) and the recoveries range from 97.0% to 102.6%. The results of international standard materials are consistent with the certified values. The proposed method solves the issues of the conventional method and improves the influence of the matrix effect and equipment drift on analytical results. This method can determine simultaneously active alumina and active silicon. The concept of active alumina can be used to comprehensively evaluate gibbsite bauxite and the adjustment of the production method of aluminum oxide.

For tin, tungsten, titanium and other elements not easily digested by acid, a suitable sample preparation method combined with Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES) can help to improve the accuracy and efficiency. Polymetallic ore samples were melted with Na2O2 and then extracted by mixed leaching solution (hydrochloric acid-tartaric acid-hydrogen peroxide).15 major and minor elements including Cu, Pb, Zn, Sn, W and Ti were determined by ICP-OES using an internal standard method. The analytical lines of analyzed elements and the correction mode of the background were optimized. The effect of crucible contamination, solvent, Al and internal standard element on the results were systematically studied. The method has detection limits of 7-995 μg/g, recoveries of 90.3%-105.0% (in Table 4), and relative standard deviations (RSD, n=12) of 0.8%-6.6% (in Table 3) by the standard addition method. For five different types of polymetallic ores, the method has a wide test range (40 μg/g to 25%).

Different pretreatment methods should be used for quick and accurate determination of major and minor elements in different types of polymetallic ores. In this study, five types of polymetallic ores including Pb-Zn, Sn, Mo, V, and Cu-Ni polymetallic ores were collected from typical metallogenic areas of China. Comparison of microwave digestion using mixed acids of hydrochloric-nitric-perchloric-hydrofluoric-sulphuric acids with digestion using nitric acid only was performed. Nine elements, Cu, Pb, Zn, Sn, W, Mo, Mn, Ti, and V were determined by the Inductively Coupled Plasma-Optical Emission Spectrometry technique. Analytical results of National Standards Reference indicate that digestion with a mixture of five acids has a long digestion time and can simultaneously determine the 6 elements of Cu, Pb, Zn, Mo, Mn, and V. Digestion with nitric acid needs only 1 h and can determine 4 elements, Cu, Pb, Zn, and Mo. Two methods have good accuracy and precision (RSD < 5%) and low detection limits of 5.3-30 mg/kg, which are suitable for application in geological analyses. However, neither of these two methods are suitable for analyzing Sn, W, and Ti, because these elements cannot be digested completely by acids.

The work involved to accurately determine the major and minor elements in tin ore, which contains large quantities of paragenetic and associated elements is extremely challenging. It is difficult to decomposed tin ore and the Sn, W and Mo are easily precipitated in HCl solution. A highly efficient analytic method which is capable of simultaneously determining Sn, W and Mo in tin ores by Inductively Coupled Plasma-Atomic Emission Spectrometry (ICP-AES) is reported in this paper. The ore samples are melted with Na2O2 and acidized by mixed tartaric acid, hydrochloric acid and hydrogen peroxide. Co is used as the internal standard element. The linear range of this present method is 0.00-40.0 mg/L, and the detection limits are 10 mg/kg for Sn, 30 mg/kg for W, 3.3 mg/kg for Mo, 12 mg/kg for Cu, 15 mg/kg for Pb and 40 mg/kg for Zn, respectively. The relative standard deviation (RSD,n=9) is less than 5.0%. All results of the National Standard Reference Materials determined by this method are consistent with the certified values. The samples can reach complete melting by using Na2O2 and the step to evaporate the HF acid to remove Si can be avoided, which results in reducing the processing time of samples. The application of mixed tartaric acid-hydrochloric acid-hydrogen peroxide system assists Sn, W and Mo to form stable complexations, which solves the problem of lower determining results due to forming precipitations of tungstenic acid, molybdic acid and stannic acid in HCl solution.

The chemical procedure of the HCl-HNO3-HF-HClO4 acids system on the electric heating plate is usually used to determine trace Ag in geochemical exploration samples using the Graphite Furnace Atomic Absorption Spectrometry (GFASS) method. Ir and Pt are usually used as the matrix modifiers. However, the thiourea serving as the medium probably leads to a large amount of precipitation with Cu, which interferes with the measurement results. Also, this dissolution method is time consuming during the analysis process and costly. In this paper it is reported that sample in water bath is dissolved by 50% aqua regia. The 50 g/L thiourea matrix modifier was added as the solution, avoiding the precipitation with Cu. The detection limit was 0.01 μg/g with good accuracy and precision. The qualified rates of internal and external examination were in agreement with the standard values. This new method simplifies the processing steps, saving time and cost, and is suitable for batch samples analyses. Furthermore, the dissolved solution of the samples was applied to determine As, Hg, Bi, Sb and other elements by the hydride generation method.

C32H33CoF12N4O8S2, monoclinic, P21/c (no. 14), a = 12.181(1) Å, b = 15.221(1) Å, c = 22.396(2) Å, β = 101.743(1)°, V = 4065.6 Å3, Z = 4, Rgt(F) = 0.0744, wRref(F2) = 0.2630, T = 291 K.

Abstract Background Bostrycin is a novel compound isolated from marine fungi that inhibits proliferation of many cancer cells. However, the inhibitory effect of bostrycin on lung cancers has not been reported. This study is to investigate the inhibitory effects and mechanism of bostrycin on human lung cancer cells in vitro. Methods We used MTT assay, flow cytometry, microarray, real time PCR, and Western blotting to detect the effect of bostrycin on A549 human pulmonary adenocarcinoma cells. Results We showed a significant inhibition of cell proliferation and induction of apoptosis in bostrycin-treated lung adenocarcinoma cells. Bostrycin treatment caused cell cycle arrest in the G0/G1 phase. We also found the upregulation of microRNA-638 and microRNA-923 in bostrycin-treated cells. further, we found the downregulation of p110α and p-Akt/PKB proteins and increased activity of p27 protein after bostrycin treatment in A549 cells. Conclusions Our study indicated that bostrycin had a significant inhibitory effect on proliferation of A549 cells. It is possible that upregulation of microRNA-638 and microRNA-923 and downregulaton of the PI3K/AKT pathway proteins played a role in induction of cell cycle arrest and apoptosis in bostrycin-treated cells.

Abstract Background Resveratrol (RES), an estrogen analog, is considered as a potential cancer chemo-preventive agent. However, it remains unclear how RES is transported into cells. In this study, we observed that Caveolin-1(CAV1) expression can increase the cytotoxic and pro-apoptotic activity of RES in a dose- and time-dependent manner both in vitro and in vivo in a Hepatocellular Carcinoma animal model. Methods High performance liquid chromatography (HPLC) demonstrated that RES intra-cellular concentration is increased about 2-fold in cells stably expressing CAV1 or CAVM1 (a scaffolding domain (81-101AA)-defective CAV1 mutant) compared to the untransduced human Hepatoblastoma cell line (HepG2) or after transduction with the green fluorescent protein (GFP) control vector. The increased intra-cellular transport of RES was abolished in cells stably expressing CAVM2 (a cholesterol shuttle domain (143-156AA)-defective CAV1 mutant) or CAVRNAi. In order to further characterize CAV1-dependent RES transport, we synthesized RES-dansyl chloride derivatives as fluorescent probes to visualize the transport process, which demonstrated a distribution consistent with that of CAV1 in HepG2 cells. Results In addition, RES endocytosis was not mediated by estrogen receptor (ER) α and β, as suggested by lack of competitive inhibition by estrogen or Tamoxifen. Pathway analysis showed that RES can up-regulate the expression of endogenous CAV1; this activates further the MAPK pathway and caspase-3 expression. Discussion This study provides novel insights about the role played by CAV1 in modulating cellular sensitivity to RES through enhancement of its internalization and trafficking.