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In this study, the antioxidant and bile salt binding activities of flavonoids from Aronia melanocarpas' leaves (AMF) by ultrasound-assisted extraction. Based on single factors (liquid-solid ratio, ethanol concentration, ultrasonic power and ultrasonic time) and response surface method, the extraction process of AMF was optimized with flavonoid yield as the index. The antioxidant activity of AMF was evaluated by DPPH·, ABTS+· scavenging rate and reduction power. The bile salt binding ability of AMF was investigated by using sodium cholate, sodium taurocholate and sodium glycocholate as binding objects. The results showed that the optimal extraction conditions were as follows: The liquid-solid ratio of 61:1 mL/g, the ethanol concentration of 60%, the ultrasonic power of 100 W, and the ultrasonic time of 59 min. Under the optimized conditions, the yield of flavonoids was 24.22%±0.29%, which approximated the predicted value (24.27%). The IC50 of DPPH and ABTS+ radicals scavenging capacities of AMF were 0.32 and 0.16 mg/mL, respectively. The AMF demonstrated the strongest reducing power with an absorbance value of 1.09 at the sample concentration of 0.5 mg/mL. The IC50 of AMF for the binding rate of sodium cholate, sodium taurocholate and sodium glycocholate were 0.76, 3.01 and 6.49 mg/mL, respectively. In summary, AMF exhibited strong antioxidant and bile salt binding capacity. This study could provide a basis for AMF to further develop natural antioxidants and hypolipidemic drugs.

Chuanxiong rhizoma (CX) has been utilized for centuries as a traditional herb to treat blood stasis syndromes. However, the pharmacological mechanisms are still not completely revealed. This research was aimed at exploring the molecular mechanisms of CX treatment for thrombosis. Network pharmacology was used to predict the potential anti-thrombosis mechanism after correlating the targets of active components with targets of thrombosis. Furthermore, we verified the mechanism of using CX to treat thrombosis via molecular docking and in vitro experiments. Network pharmacology results showed that a total of 18 active ingredients and 65 targets of CX treatment for thrombosis were collected, including 8 core compounds and 6 core targets. We revealed for the first time that tissue factor (TF) had a close relationship with most core targets of CX in the treatment of thrombosis. TF is a primary coagulation factor in physiological hemostasis and pathological thrombosis. Furthermore, core components of CX have strong affinity for core targets and TF according to molecular docking analysis. The in vitro experiments indicated that Ligustilide (LIG), the representative component of CX, could inhibit TF procoagulant activity, TF mRNA and protein over-expression in a dose-dependent manner in EA.hy926 cells through the PI3K/Akt/NF-κB signaling pathway. This work demonstrated that hemostasis or blood coagulation was one of the important biological processes in the treatment of thrombosis with CX, and TF also might be a central target of CX when used for treating thrombosis. The inhibition of TF might be a novel mechanism of CX in the treatment of thrombosis.

Camellia fascicularis is a unique plant rich in bioactive components. However, the isolation of the active substances in C. fascicularis leaves via sequential extraction with solvents of different polarity and the determination of their antioxidant and antitumor activities have not been reported. In this study, the total methanol extract of C. fascicularis leaves was sequentially extracted with different polar solvents, and the corresponding petroleum ether extract (PEE), ethyl acetate extract (EAE), and water extract (WE) were analyzed for their contents in active substances such as flavonoids, polyphenols, polysaccharides, and saponins. The antioxidant ability of the polar extracts was investigated by determining their reducing power and the radical scavenging rate on 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and hydroxyl radicals, and CCK-8 and Annexin-FITC/propidium iodide staining assays were conducted to investigate their inhibitory effects on HCCLM6 and HGC27 tumor cells. The results showed that PEE had a high saponin content of 197.35 ± 16.21 mg OAE/g, while EAE and WE exhibited a relatively higher polysaccharide content of 254.37 ± 1.99 and 373.27 ± 8.67 mg GE/g, respectively. The EAE demonstrated the greatest reducing power and the strongest clearing abilities on ABTS and DPPH radicals with respective EC50 values of 343.45 ± 20.12 and 14.07 ± 0.06 μg/ml. Moreover, the antitumor ability of the different polar extracts was dose-dependent, with WE showing the most potent inhibitory ability against HCCLM6 and HGC27 cells.

The affine Deligne–Lusztig variety $X_w(b)$ in the affine flag variety of a reductive group ${\mathbf G}$ depends on two parameters: the $\sigma $ -conjugacy class $[b]$ and the element w in the Iwahori–Weyl group $\tilde {W}$ of ${\mathbf G}$ . In this paper, for any given $\sigma $ -conjugacy class $[b]$ , we determine the nonemptiness pattern and the dimension formula of $X_w(b)$ for most $w \in \tilde {W}$ .

We prove a character formula for some closed fine Deligne–Lusztig varieties. We apply it to compute fixed points for fine Deligne–Lusztig varieties arising from the basic loci of Shimura varieties of Coxeter type. As an application, we prove an arithmetic intersection formula for certain diagonal cycles on unitary and GSpin Rapoport–Zink spaces arising from the arithmetic Gan–Gross–Prasad conjectures. In particular, we prove the arithmetic fundamental lemma in the minuscule case, without assumptions on the residual characteristic.

In this paper, we introduce the Newton decomposition on a connected reductive $p$ -adic group $G$ . Based on it we give a nice decomposition of the cocenter of its Hecke algebra. Here we consider both the ordinary cocenter associated to the usual conjugation action on $G$ and the twisted cocenter arising from the theory of twisted endoscopy. We give Iwahori–Matsumoto type generators on the Newton components of the cocenter. Based on it, we prove a generalization of Howe’s conjecture on the restriction of (both ordinary and twisted) invariant distributions. Finally we give an explicit description of the structure of the rigid cocenter.