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Coccidiosis is the costliest disease in the rabbit industry. The kits do not obtain maternal immunity against the diseases and therefore suckling to weaning period being the riskiest time of infection. To date, control of coccidiosis is relied on the use of chemical coccidiostat as a drug or as the substance in feed. Whilst, continuous exposure to antibiotics develops resistance and contamination in the carcass. There is no commercially provided vaccine to prevent rabbit coccidiosis cases. In this report, we processed precocious lines from the strain of Yogyakarta origin by using selection pressure to attenuate Eimeria spp. The vaccine candidate composed of 50% of each Eimeria media and Eimeria intestinalis were given orally to initiate protective immunity against rabbit coccidiosis. The vaccinated groups with 5 x 102 oocyst dosage exhibited 95% less total oocyst excretion compared to the non-vaccinated group. Our experiment showed no mortality and without any significantly detrimental response of vaccinated rabbits during the observation time (P

The Androgen Receptor (AR) Gene’s expression is essential during puberty and testes maturation, which is also used as a reference for Turkey’s breeding program. Therefore, this study aims to develop and optimize two primer sets for studying relative expression using qPCR technology. The primers were designed to amplify specific regions in the AR gene as the main target, and the β-actin gene as an internal control. They were tested using in-silico and amplicon sequencing, as well as efficiency calculation with a constructed standard curve from serially diluted reactions. Based on the sequencing methods, the primers amplified the corresponding regions of the respective targets. The primer for AR gene had an efficiency of 98.03%, a slope of -3.37, and an R2 of 0.995, while that of the β-actin gene had an efficiency of 98.01%, a slope of -3.371, and an R2 of 0.999. The two efficiencies exceeded the standard (93 -103%) value and the melting curve analysis showed that no non-specific amplification was discovered for both primers. According to the tests, the primers are suggested as acceptable to be used for the relative expression study of the AR gene in Turkey.

The success of animal husbandry is supported by good animal and environmental health management practices. This study examined the correlation between the sheep farmer’s characteristics and the animal and environmental health management practices. The data collected by direct interviews with 48 respondents in Batur Village, Banjarnegara. Indonesia. The aspects of animal (exercise, grooming, wool shearing, supplement feeding, and water drinking) and environmental (sheepfold sanitation, waste processing, and waste removal) health management practices were divided into three categories, namely low, medium, and high levels. The results showed that 83.3% of respondents were in the low-level following by medium (14.6%) and high (2.1%) levels of animal health management. Furthermore, on the environmental health management shows, 27.1% of respondents were in the low level, 60.4% in the medium level, and 12.5% in the high level. There was a significant relationship between farmers’ characteristics (informal education, livestock farming experience, and the number of sheep) and animal health management practice. A significant relationship was also shown between formal education, livestock purpose, livestock experience, and number of sheep by farmers toward environmental health management practice. It may conclude that the low and medium levels of animal and environmental health management practices were dominant in Batur Village.