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Background & Aims: Urinary tract infections (UTI) are a leading cause of bacterial infections in humans.The widespread use of antibiotics has resulted in the emergence of antibiotic-resistant microorganisms. Hence, the current study was conducted to investigate the antibiotic sensitivity pattern of uropathogens in UTI. Materials & Methods: A total of 642 urine samples were collected from suspected UTI patients and tested microbiologically. Antimicrobial susceptibility tests were performed for the isolated pathogens using the Kirby- Bauer disk diffusion method. Results: Out of 642 urine samples, 308 (48%) were found to exhibit significant bacteriuria. Females had a higher rate of UTI (68%) than males (32%), with a higher prevalence in the middle-aged group, while males reported a higher prevalence in the elderly group, which was statistically significant. The most common organism was Escherichia coli (57.2%), followed by Klebsiella pneumoniae (26.3%), Pseudomonas aeruginosa (8.4%), Proteus spp. (3.6%), Enterococcus spp. (2.6%), and Staphylococcus aureus (1.9%). UTI were more common in middle-aged female patients (31 to 45 years), while in males, high prevalence was seen in older patients (>45 years). Meropenem, Gentamicin, Nitrofurantoin, and Co-Trimoxazole were amongst the most sensitive drugs against E.coli and K.pneumoniae. Conclusion:Due to the irrational and injudicious use of antibiotics, commonly isolated uropathogens have a changing resistance pattern, resulting in reduced treatment effectiveness. This could be overcome by routine antimicrobial resistance surveillance, antimicrobial stewardship measures, and culture-guided therapy.

Abstract The sandalwood (Santalum album) tree holds significant commercial and cultural value globally, particularly in India. Among the 17 species within the Santalum genus, Santalum album stands out due to its high oil content and the presence of favorable compounds such as α-and β-santalols. The wood of this species is highly prized for carving and its aromatic heartwood oil is widely used in attar, perfumes, cosmetics, soaps, toiletries, medicinal products, flavored tobacco, and areca nut products. Despite its historical significance, sandalwood is vulnerable and endangered due to legal policies, illegal harvesting, grazing, lopping, natural fires, exotic plantations, sandalwood spike disease, and climate change. The combination of increased demand and declining supply has led to a surge in sandalwood prices, attracting attention from farmers, policymakers, and the industry sector, prompting interest in commercial cultivation. This has sparked global involvement from various countries, government agencies, industries, research disciplines, industry representatives, and sandalwood growers. This article offers a thorough review of sandalwood utilization, legal issues, and management challenges. It draws on literature and expert insights to assess current practices and research findings. The study identifies critical factors such as the number of hosts, host type, and planting geometry that influence successful establishment and production in farmers' fields. It also highlights obstacles and opportunities for the future sustainability of sandalwood, aiming to guide effective management and policy strategies for this valuable resource. The government of India has initiated policy-level changes regarding the ownership, cultivation, and harvesting of sandalwood trees, encouraging many farmers to cultivate on private land even beyond its natural boundaries. However, the complexity of sandalwood cultivation requires further scientific introspection to ensure successful establishment and sustainable management in the future.

Introduction: Acute encephalitis syndrome (AES) is one of the important causes of mortality among children in India. Active management of the cases, followed by addressing the cause of AES, is the key strategy for preventing mortality. Lack of laboratory facility and difficulty of sampling blood and cerebrospinal fluid (CSF) for assessing causes is one of the important barriers to early initiation of treatment. The main objective of the study is to validate the Scrub Typhus Encephalitis Assessment Tool (SEAT) for the management of AES. Methods: The study is a continuation of a study conducted in a tertiary care hospital in Eastern Uttar Pradesh. A machine learning (LightGBM) model was built to predict the probability of scrub typhus diagnosis among patients with acute encephalitis. Three models were built: one with sociodemographic characters, the second with Model 1 variables and blood parameters, and the third with Model 2 variables and CSF parameters. Results: The sensitivity of diagnosing the scrub typhus case was 71%, 77.5%, and 83% in Model 1, Model 2, and Model 3, respectively, and specificity was 61.5%, 75.5%, and 76.3%, respectively, in the models. In Model 1 fever duration, in Models 2 and 3, neutrophil/lymphocyte ratio was the most important predictor for differentiating the scrub and nonscrub cases. Conclusion: With the available sensitivity and specificity of the tool, the SEAT can be a valuable tool for the prediction of scrub typhus as a cause of AES cases in remote areas.

Abstract What nutrient supply options can ensure maximum productivity and optimize the nutrient uptake of rice–wheat system (R-W system)? an experiment started in the year 1998 (19-year-old) to examine the impact of optimal nutrient supply (NS) strategies to maximize crop productivity and nutritional security in R-W system. To determine the best nutrient management strategies (BNMS), seven different NS methods were tested. These included (organic and mineral fertilizers), as well as combinations such as integrated plant nutrition system (IPNS), IPNS + B (berseem), and IPNS + C (cowpea), with the aim of enhancing the productivity and nutrient absorption of R-W system. Results showed that rice grain yield notably wide-ranging from 1.61 to 5.81 t ha−1 under different NS options and highest rice grain yield (mean of 19 years) was observed at IPNS + C (5.81 t ha−1), which was at par with IPNS (5.79 t ha−1), STCR (soil test crop response) (5.76 t ha−1) and IPNS + B (5.67 t ha−1) followed by 100% recommended dose fertilizer NPK (4.41 t ha−1), which equality with OF (organic farming) (4.04 t ha−1) and lowest was recorded in control plot (1.61 t ha−1). Wheat grain yield varied significantly from 1.43 to 5.86 t ha−1 under different NS options. The highest yield (mean of 19 years) was observed in treatments IPNS + B (5.86 t ha−1), IPNS (5.77 t ha−1), IPNS + C (5.48 t ha−1), and STCR (5.45 t ha−1), followed by OF at 4.49 t ha−1, NPK at 3.76 t ha−1, with the lowest in the control plot at 1.43 t ha−1. Additionally, total phosphorus and sulfur accumulation in rice (grain: 8.41 to 39.09 kg ha−1; straw: 6.02 to 23.54 kg ha−1) varied significantly across nutrient supply treatments. Overall, adoption of IPNS integration with legumes (IPNS + B and IPNS + C), can significantly improve productivity and nutrient accumulation in R-W systems. Incorporating legumes into farming practices is advised for sustained productivity and nutritional benefits.

Abstract Background Lipids produced using oleaginous yeast cells are an emerging feedstock to manufacture commercially valuable oleochemicals ranging from pharmaceuticals to lipid-derived biofuels. Production of biofuels using oleaginous yeast is a multistep procedure that requires yeast cultivation and harvesting, lipid recovery, and conversion of the lipids to biofuels. The quantitative recovery of the total intracellular lipid from the yeast cells is a critical step during the development of a bioprocess. Their rigid cell walls often make them resistant to lysis. The existing methods include mechanical, chemical, biological and thermochemical lysis of yeast cell walls followed by solvent extraction. In this study, an aqueous thermal pretreatment was explored as a method for lysing the cell wall of the oleaginous yeast Rhodotorula toruloides for lipid recovery. Results Hydrothermal pretreatment for 60 min at 121 °C with a dry cell weight of 7% (w/v) in the yeast slurry led to a recovery of 84.6 ± 3.2% (w/w) of the total lipids when extracted with organic solvents. The conventional sonication and acid-assisted thermal cell lysis led to a lipid recovery yield of 99.8 ± 0.03% (w/w) and 109.5 ± 1.9% (w/w), respectively. The fatty acid profiles of the hydrothermally pretreated cells and freeze-dried control were similar, suggesting that the thermal lysis of the cells did not degrade the lipids. Conclusion This work demonstrates that hydrothermal pretreatment of yeast cell slurry at 121 °C for 60 min is a robust and sustainable method for cell conditioning to extract intracellular microbial lipids for biofuel production and provides a baseline for further scale-up and process integration. Graphical abstract

Abstract Oilcane—an oil‐accumulating crop engineered from sugarcane—and microbial oil have the potential to improve renewable oil production and help meet the expected demand for bioderived oleochemicals and fuels. To assess the potential synergies of processing both plant and microbial oils, the economic and environmental implications of integrating microbial oil production at oilcane and sugarcane biorefineries were characterized. Due to decreased crop yields that lead to higher simulated feedstock prices and lower biorefinery capacities, current oilcane prototypes result in higher costs and carbon intensities than microbial oil from sugarcane. To inform oilcane feedstock development, we calculated the required biomass yields (as a function of oil content) for oilcane to achieve financial parity with sugarcane. At 10 dw% oil, oilcane can sustain up to 30% less yield than sugarcane and still be more profitable in all simulated scenarios. Assuming continued improvements in microbial oil production from cane juice, achieving this target results in a minimum biodiesel selling price of 1.34 [0.90, 1.85] USD∙L−1 (presented as median [5th, 95th] percentiles), a carbon intensity of 0.51 [0.47, 0.55] kg CO2e L−1, and a total biodiesel yield of 2140 [1870, 2410] L ha−1 year−1. Compared to biofuel production from soybean, this outcome is equivalent to 3.0–3.9 as much biofuel per hectare of land and a 57%–63% reduction in carbon intensity. While only 20% of simulated scenarios fell within the market price range of biodiesel (0.45–1.11 USD∙L−1), if the oilcane biomass yield would improve to 25.6 DMT∙ha−1∙y−1 (an equivalent yield to sugarcane) 87% of evaluated scenarios would have a minimum biodiesel selling price within or below the market price range.

Abnormal accumulation of insoluble α-synuclein (α-Syn) inclusions in neurons, neurites, and glial cells is the defining neuropathology of synucleinopathies, including Parkinson's disease (PD), dementia with Lewy bodies (DLB), and multiple system atrophy. Accumulation of α-Syn inclusions in the amygdala has been well-documented in post-mortem studies of PD and DLB brains, as well as preclinical animal models of these conditions. Though α-Syn pathology is closely associated with neurodegeneration, there is a poor correlation between neuronal loss in the amygdala and the clinical features of PD and DLB. Moreover, functional interaction between the cerebral cortex and the amygdala is critical to regulating emotion, motivation, and social behaviors. The cortico-amygdala functional interaction is likely to be disrupted by the development of α-Syn pathology in the brain. Thus, we hypothesize that neuronal α-Syn inclusions disrupt cortical modulation of the amygdala circuits and are sufficient to drive social behavioral deficits. In the present work, we designed a series of longitudinal studies to rigorously measure the time courses of neurodegeneration, functional impairment of cortico-amygdala connectivity, and development of amygdala-dependent social behavioral deficits to test this hypothesis. We injected α-Syn preformed fibrils (PFFs) into the dorsal striatum to induce α-Syn aggregation in the amygdala and the medial prefrontal cortex (mPFC) of C57BL6 mice of both sexes, followed by a detailed analysis of temporal development of α-Syn pathology, synaptic deficits, and neuronal loss in the amygdala, as well as behavioral deficits at 3–12 months post injections. Development of α-Syn inclusions caused losses of cortical axon terminals and cell death in the basolateral amygdala (BLA) at 6- and 12-months post injections, respectively. At a relatively early stage of 3 months post injections, the connection strength of the mPFC-BLA synapse was decreased in PFFs-injection mice compared to controls. Meanwhile, the PFFs-injected mice showed impaired social interaction behavior, which was rescued by chemogenetic stimulation of mPFC-BLA connections. Altogether, we presented a series of evidence to delineate circuit events in the amygdala associated with the accumulation of α-Syn inclusions in the mouse brain, highlighting that functional impairment of the amygdala is sufficient to cause social behavior deficits. The present work further suggests that early circuit modulation could be an effective approach to alleviate symptoms associated with α-Syn pathology, necessitating studies of functional consequences of α-Syn aggregation.

Peritoneal carcinomatosis (PC), the spread of cancer cells in the peritoneum, is a significant concern in advanced gastrointestinal and gynecological cancers. This case series includes findings on the appearance and pattern of PC on 18F-fluorodeoxyglucose positron emission tomography/CT (18F-FDG PET/CT). The primary sources of peritoneal dissemination are direct invasion from abdominal or pelvic tumors and metastatic spread from distant tumors. The accurate preoperative diagnosis and quantification of PC play a vital role in determining the appropriate treatment approach, with a particular emphasis on surgical planning. Several imaging modalities have been employed in preoperative evaluation, such as computed tomography (CT), magnetic resonance imaging (MRI), and 18F-FDG PET/CT. Among these modalities, 18F-FDG PET/CT has demonstrated improved anatomical localization and accurate information about the nature of pathological findings. The case series showcases four cases that illustrate the imaging characteristics of PC on FDG PET/CT. FDG PET/CT plays a vital role in diagnosing and assessing PC, aiding in its detection, staging, and treatment planning. It surpasses conventional imaging techniques in identifying and characterizing lesions and detecting the primary tumor site in cases where its location is unknown. Furthermore, FDG PET/CT additionally assists in evaluating treatment response and monitoring disease progression, providing insights into treatment effectiveness and guiding patient management decisions.

The lightness of an object is an intrinsic property that depends on its surface reflectance spectrum. The visual system estimates an object's lightness from the light reflected off its surface. However, the reflected light also depends on object extrinsic properties of the scene, such as the light source. For stable perception, the visual system needs to discount the variations due to the object extrinsic properties. We characterize this perceptual stability for variation in two spectral properties of the scene: the reflectance spectra of background objects and the intensity of light sources. We measure human observers’ thresholds of discriminating computer-generated images of 3D scenes based on the lightness of a spherical target object in the scene. We measured change in discrimination thresholds as we varied the reflectance spectra of the objects and the intensity of the light sources in the scene, both individually and simultaneously. For small amounts of extrinsic variations, the discrimination thresholds remained nearly constant indicating that the thresholds were dominated by observers’ intrinsic representation of lightness. As extrinsic variation increased, it started affecting observers’ lightness judgment and the thresholds increased. We estimated that the effects of extrinsic variations were comparable to observers’ intrinsic variation in the representation of object lightness. Moreover, for simultaneous variation of these spectral properties, the increase in threshold squared compared to the no-variation condition was a linear sum of the corresponding increase in threshold squared for the individual properties, indicating that the variations from these independent sources combine linearly.

Purpose – The study aims to examine how the information disclosed by the managers in the management discussion and analysis (MD&A) reports varies at the different levels of corporate performance. Design/methodology/approach – To understand this quantile effect, first OLS technique was adopted and then, the quantile regression method was applied to explore the impact of MD&A disclosures on the firm performance across the lower and upper quantiles. The sample size for the study is 490 firms’ year observations for the period 2016–2022. Findings – The results of the study demonstrate the negative but significant relationship between MD&A disclosures and corporate performance, supporting the two management strategies of “competitive disadvantage” in case of good performance and “management impression strategy” in case of poor performance. Furthermore, with other corporate governance variables, both the size of the board and the number of independent directors on the board are positively significant only in the case of the upper quantile indicating the heterogeneity in the relationship between the performance and the MD&A disclosures. Therefore, the overall findings of the study support that these results contradict the agency theory and the stakeholders’ theory as managers are not acting well as agents on behalf of the investors and work well only when they are controlled by the large board having more independent directors. Originality/value – To the best of the authors’ knowledge, no study so far has incorporated quantile regression to assess the effect of MD&A disclosures on company performance at various levels of the firm performance, which gives more robust insights about the viewpoint of the managers on the different level of the firm performance. In other words, this study highlights the important information as to how the information provided in the MD&A reports varies as per the good or poor performance of the companies.

Abstract In the present study, three varieties of kidney beans (Red Rajma (RR), Rajma Chitra (RC), and Rajma Sharmili (RS)) were physically characterized and the effect of pre-gelatinization by microwave and hot water on functional and pasting properties of flour were studied. A significant difference was observed in the physical properties of all the kidney beans, indicating that they can be easily characterized and identified based on physical properties. Pre-gelatinization treatment led to variation in color, functional and pasting properties of flours. Color parameters L*, b*, chroma, and hue values reduced with the treatment, whereas a* and total color difference ( $$\Delta$$ Δ E) values increased. Water absorption capacity (WAC) and oil absorption capacity (OAC) varied in the range of 1.15–1.91% and 1.25–1.75% for microwave and hot water treatment, respectively. The highest value of WAC and OAC was observed for microwave-treated RC and hot water-treated RR, respectively. WAC increases with both treatments, whereas OAC increases only with water bath cooking. Functional group analysis (FTIR) showed no major peaks shifting in water bath treatment, whereas a slight deflation of certain peaks was observed in the case of microwave treatment. Pasting curves showed a significant reduction in peak and final viscosity after pre-gelatinization treatment; however, none of the samples showed any breakdown during cooking. Graphical Abstract

ABSTRACT Sepsis and chronic infections with Pseudomonas aeruginosa, a leading “ESKAPE” bacterial pathogen, are associated with increased morbidity and mortality and skeletal muscle atrophy. The actions of this pathogen on skeletal muscle remain poorly understood. In skeletal muscle, mitochondria serve as a crucial energy source, which may be perturbed by infection. Here, using the well-established backburn and infection model of murine P. aeruginosa infection, we deciphered the systemic impact of the quorum-sensing transcription factor MvfR (multiple virulence factor regulator) by interrogating, 5 days post-infection, its effect on mitochondrial-related functions in the gastrocnemius skeletal muscle and the outcome of the pharmacological inhibition of MvfR function and that of the mitochondrial-targeted peptide, Szeto-Schiller 31 (SS-31). Our findings show that the MvfR perturbs adenosine triphosphate generation, oxidative phosphorylation, and antioxidant response, elevates the production of reactive oxygen species, and promotes oxidative damage of mitochondrial DNA in the gastrocnemius muscle of infected mice. These impairments in mitochondrial-related functions were corroborated by the alteration of key mitochondrial proteins involved in electron transport, mitochondrial biogenesis, dynamics and quality control, and mitochondrial uncoupling. Pharmacological inhibition of MvfR using the potent anti-MvfR lead, D88, we developed, or the mitochondrial-targeted peptide SS-31 rescued the MvfR-mediated alterations observed in mice infected with the wild-type strain PA14. Our study provides insights into the actions of MvfR in orchestrating mitochondrial dysfunction in the skeletal murine muscle, and it presents novel therapeutic approaches for optimizing clinical outcomes in affected patients.IMPORTANCESkeletal muscle, pivotal for many functions in the human body, including breathing and protecting internal organs, contains abundant mitochondria essential for maintaining cellular homeostasis during infection. The effect of Pseudomonas aeruginosa (PA) infections on skeletal muscle remains poorly understood. Our study delves into the role of a central quorum-sensing transcription factor, multiple virulence factor regulator (MvfR), that controls the expression of multiple acute and chronic virulence functions that contribute to the pathogenicity of PA. The significance of our study lies in the role of MvfR in the metabolic perturbances linked to mitochondrial functions in skeletal muscle and the effectiveness of the novel MvfR inhibitor and the mitochondrial-targeted peptide SS-31 in alleviating the mitochondrial disturbances caused by PA in skeletal muscle. Inhibiting MvfR or interfering with its effects can be a potential therapeutic strategy to curb PA virulence.

Abstract Concerns about climate change and the reliance on fossil fuel reserves have motivated researchers to identify new renewable sources of energy. Biomass holds the potential to replace fossil‐derived products with biofuels and bio‐based chemicals. Plant‐derived lipids are promising sources of biofuels; however, the production of plant oil often leads to the release of massive amounts of carbon dioxide due to deforestation and land‐use change. The production of biofuels via plant oils (such as soybean) also competes with food production and in turn, impacts biodiversity. To mitigate these issues, the production of lipids from oleaginous yeasts could be an excellent alternative by incorporating these microbes into biorefineries utilizing agricultural or forest residues. Eventually, these microbial lipids could be potential sources for producing lipid‐derived biofuels. However, the current conventional methods for the production and recovery of lipids from oleaginous microbes suffer from economic and ecological challenges that affect its industrial‐scale expansion. This review highlights the major economic and environmental bottlenecks for the production of lipid‐derived biofuels from oleaginous yeasts. It also provides perspectives on the strategies that could be adapted on economic and ecological fronts to assist the expansion of the production of microbial lipid‐derived biofuels at an industrial scale.

The present study evaluated the effect of various pre-treatments (Potassium metabisulphite (KMS), KMS+Ultrasonication (KMSUS), KMS+Microwave (KMSMW), KMS+Hot water (KMSHWB) followed by infrared drying on the quality of Elephant Foot Yam (EFY). KMSMW-treated samples showed the fastest drying rate and highest moisture diffusivity, whereas the KMS-treated sample had the highest weight gain (171.57 %) in rehydration (80 °C). KMSMW and KMSHWB-treated samples exhibited softer textures in rehydration compared to control. Pre-treated samples had higher water absorption capacity and lower oil absorption capacity compared to the control. Pre-treatments also influenced the amylose content and the highest amylose content was observed for KMSUS (21.25 %). Pre-treatments positively influenced the retention of bioactive compounds and reduced the calcium oxalate (117.37 mg/g to 64.09 mg/g). Among all the pre-treatments, KMSHWB showed higher retention of bioactive compounds viz., TPC (297.67 mg GAE/100 g), TFC (922.22 mg QE/100 g), DPPH scavenging activity (57.91 %), ABTS (1.42 mmol TE/100 g) and lowest calcium oxalate (64.09 mg/100 g).

The present study focuses on the utilization of non-conventional starch (NS) derived from guinea grass seed to produce porous starch (PS) and octenyl succinic anhydride-modified PS (OSAPS). The study also introduces a novel approach to produce PS, aiming to overcome the high cost associated with glucoamylase. The formation of porous granules was successfully achieved through the combination of alpha-amylase and ultrasonication, as confirmed by electron micrographs and analysis of pore size and specific surface area. The results demonstrate that OSAPS exhibits superior emulsion stability, well-defined pore structures, and desirable amphiphilic characteristics. All three starches were loaded with lycopene, flaxseed oil, and a combination of both to prepare microcapsules. The presence of flaxseed oil within the starch matrix was confirmed through micrographs and functional group analysis. Notably, OSAPS exhibited the highest encapsulation efficiency at 74.80 %, along with a lycopene retention rate of up to 27.32 mg/100 g. Moreover, OSAPS showcased favorable antioxidant activity (89.29 %), phenolic content (0.45 mg GAE/g), and flavonoid content (97.61 mg QE/100 g). These findings underscore the remarkable potential of OSAPS as a delivery system for bioactive compounds, particularly for the co-encapsulation of oils and pigments.

The aim of this study is to examine the impact of corporate characteristics on voluntary disclosures of management discussion and analysis (MD&A) reports in India. Using a formal tone, the data was extracted from the annual reports of the top 100 listed firms available on the CMIE Prowess database for seven years (2016–2022). After excluding 23 companies from the financial and insurance sector, a panel regression method with the assistance of Gretl software was employed to investigate the relationship between the Management Discussion and Analysis Disclosure Index (MDADI) for voluntary aspects and various corporate attributes, with a total of 490 firm years of balanced observations. In India, firms follow the mandatory compliance of the MD&A reports, but voluntary disclosures are somehow those which are not much emphasized but are a good indication of firm performance and their accountability towards their stakeholders (Mayew et al., 2015). Our empirical findings reveal that profitability as a proxy to firm performance has a significant positive relationship with MD&A voluntary disclosures. Further, an insignificant association between VDS (Voluntary Disclosure Score) and the board size, presence of independent directors and firm size was found. This indicates that firm performance plays a significant role in adding more voluntary disclosures in MD&A reports. The possible reason for this could be the use of “Management Impression Strategy” in the MD&A reports, which means managers disclose more only when the firm has earned more and use impressive language to attract stakeholders. The outcomes of this research offer valuable insights for regulators, policymakers, and listed companies in India, aiding in the enhancement of MD&A reporting quality. Additionally, this study provides a roadmap for future research on MD&A reporting quality and corporate attributes in other emerging countries that have similar regulatory frameworks. This paper makes a timely and pertinent contribution to the scholarly discourse by shedding light on the relationship between MD&A disclosures and firm attributes. Its findings provide valuable insights for both academia and industry.

Aim: The aim and objective of this in vitro study was to evaluate the antibacterial efficacy of mineral trioxide aggregate, bioactive glass sealer, and epoxy-resin–based sealer. Materials and Methods: In the present study, 22 Mueller Hinton agar (MH agar) plates were employed and equally divided into two groups. Three holes were made by removal of agar at equidistant points and filled with root canal sealers. The strains of the bacteria used in this study were S. aureus (ATCC 6538) and C. albicans (ATCC 10231) and were divided into two groups and root canal sealers were divided into three subgroups: mineral trioxide aggregate (MTA) fillapex Sealer, Nishika Bioactive Glass sealer, and Syntex Epoxy Resin base sealer. For Staphylococcus aureus, peptone water was placed in a 2 mL test tube and bacteria were extracted from blood agar plates using a nichrome wire loop and poured into the peptone water–containing test tube and incubated for 2 hours and for C. albicans, fungi were grown at 37°C for 24 hours in MH Broth and seeded into MH agar to produce turbidity of 0.5 on the McFarland scale, which corresponds to a concentration of 108 CFU/mL. This MH broth was used as a second layer. The seeded agar was then added over the plates immediately after the insertion of sealer cement. After incubation, the diameters of zones of inhibition around the plates were measured. Results: The results of this study showed that the highest inhibition was recorded in Syntex sealer against Staphylococcus aureus followed by MTA fillapex sealer and Nishika sealer, whereas MTA fillapex showed the highest inhibition against C. albicans followed by Syntex sealer and Nishika sealer. Conclusion: Syntex sealer exhibits better antibacterial efficacy against Staphylococcus aureus and MTA fillapex exhibit better antibacterial efficacy against C. albicans.

Introduction: Seizure is the most common paediatric neurological condition with an increased predilection for early childhood. The recent definition of epilepsy includes first-onset seizures with an increased probability of future episodes. Thus, stringent evaluation of the initial episode of seizure is essential. Hence, this study estimated the aetiology, clinical profile, treatment and outcome of the first seizure episode in children at a tertiary hospital. Materials and Methods: This prospective observational study was carried out at a tertiary hospital in Barabanki, India. Children in the age group of 1 month to 12 years who presented with the first seizure episode and those admitted for other illnesses and developed the first seizure episode during hospital stay were included. Data collected were sociodemographics, clinical presentations, laboratory tests, brain imaging studies, electroencephalography (EEG), diagnosis, treatment and short-term outcomes. Results: A total of 113 patients were enrolled presenting with first-onset seizures. There were 78 (69%) males, 86 (76.1%) were below 5 years of age and 60 (53.1%) presented with fever. Most seizures had a generalised onset (58.4%). Febrile seizures (29.2%) were the most common type, followed by neurocysticercosis (20.3%). Central nervous system (CNS)-infective aetiology (58.7%) was the most common etiological classification. Abnormal findings were observed in cerebrospinal fluid examination of 24 out of 56, in brain imaging of 53 out of 80 and in EEG of 70 out of 113 cases examined. Anti-seizure medication (ASM) was prescribed to 68 patients, of which 10 had a recurrence in the 6-month follow-up period. Conclusion: The first episode of seizure in children usually had a generalised onset, with younger children of the male sex at an increased risk. CNS infections and febrile seizures were common causes. Complete history and neurological evaluation can guide the relevant investigations. ASM tailored to the aetiology controls the seizures in most cases.

Abstract Autosomal dominant pathogenic mutations in Leucine-rich repeat kinase 2 (LRRK2) cause Parkinson’s disease (PD). The most common mutation, G2019S-LRRK2, increases the kinase activity of LRRK2 causing hyper-phosphorylation of its substrates. One of these substrates, Rab10, is phosphorylated at a conserved Thr73 residue (pRab10), and is one of the most abundant LRRK2 Rab GTPases expressed in various tissues. The involvement of Rab10 in neurodegenerative disease, including both PD and Alzheimer’s disease makes pinpointing the cellular and subcellular localization of Rab10 and pRab10 in the brain an important step in understanding its functional role, and how post-translational modifications could impact function. To establish the specificity of antibodies to the phosphorylated form of Rab10 (pRab10), Rab10 specific antisense oligonucleotides were intraventricularly injected into the brains of mice. Further, Rab10 knock out induced neurons, differentiated from human induced pluripotent stem cells were used to test the pRab10 antibody specificity. To amplify the weak immunofluorescence signal of pRab10, tyramide signal amplification was utilized. Rab10 and pRab10 were expressed in the cortex, striatum and the substantia nigra pars compacta. Immunofluorescence for pRab10 was increased in G2019S-LRRK2 knockin mice. Neurons, astrocytes, microglia and oligodendrocytes all showed Rab10 and pRab10 expression. While Rab10 colocalized with endoplasmic reticulum, lysosome and trans-Golgi network markers, pRab10 did not localize to these organelles. However, pRab10, did overlap with markers of the presynaptic terminal in both mouse and human cortex, including α-synuclein. Results from this study suggest Rab10 and pRab10 are expressed in all brain areas and cell types tested in this study, but pRab10 is enriched at the presynaptic terminal. As Rab10 is a LRRK2 kinase substrate, increased kinase activity of G2019S-LRRK2 in PD may affect Rab10 mediated membrane trafficking at the presynaptic terminal in neurons in disease.

Abstract Background Oleaginous yeasts are a promising candidate for the sustainable conversion of lignocellulosic feedstocks into fuels and chemicals, but their growth on these substrates can be inhibited as a result of upstream pretreatment and enzymatic hydrolysis conditions. Previous studies indicate a high citrate buffer concentration during hydrolysis inhibits downstream cell growth and ethanol fermentation in Saccharomyces cerevisiae. In this study, an engineered Rhodosporidium toruloides strain with enhanced lipid accumulation was grown on sorghum hydrolysate with high and low citrate buffer concentrations. Results Both hydrolysis conditions resulted in similar sugar recovery rates and concentrations. No significant differences in cell growth, sugar utilization rates, or lipid production rates were observed between the two citrate buffer conditions during batch fermentation of R. toruloides. Under fed-batch growth on low-citrate hydrolysate a lipid titer of 16.7 g/L was obtained. Conclusions Citrate buffer was not found to inhibit growth or lipid production in this engineered R. toruloides strain, nor did reducing the citrate buffer concentration negatively affect sugar yields in the hydrolysate. As this process is scaled-up, $131 per ton of hydrothermally pretreated biomass can be saved by use of the lower citrate buffer concentration during enzymatic hydrolysis. Graphical Abstract

Since May 2022, mpox has been identified in 108 countries without endemic disease; most cases have been in gay, bisexual, or other men who have sex with men. To determine number of missed cases, we conducted 2 studies during June–September 2022: a prospective serologic survey detecting orthopoxvirus antibodies among men who have sex with men in San Francisco, California, and a retrospective monkeypox virus PCR testing of swab specimens submitted for other infectious disease testing among all patients across the United States. The serosurvey of 225 participants (median age 34 years) detected 18 (8.0%) who were orthopoxvirus IgG positive and 3 (1.3%) who were also orthopoxvirus IgM positive. The retrospective PCR study of 1,196 patients (median age 30 years; 54.8% male) detected 67 (5.6%) specimens positive for monkeypox virus. There are likely few undiagnosed cases of mpox in regions where sexual healthcare is accessible and patient and clinician awareness about mpox is increased.

Objectives: Following the COVID-19 pandemic, global epidemiological trends demonstrate a return to pre-pandemic levels of respiratory syncytial virus (RSV) and influenza (Flu) A/B viruses. For the appropriate clinical management of viral infections, reliable and timely diagnosis is crucial. The clinical presentation of these respiratory viral infections shows significant overlaps; thus, the syndromic diagnosis of these infections becomes challenging. The goal of this study was to compare the performance of three multiplex real-time PCR-based platforms for the detection of SARS-CoV-2, Flu A, Flu B, and RSV. Materials and Methods: A retrospective study was performed on 200 de-identified nasopharyngeal and oropharyngeal specimens. All samples were tested simultaneously on three PCR-based platforms for the detection of SARS-CoV-2, Flu A, Flu B, and RSV: HealthTrackRx’s real-time PCR Open Array® respiratory panel, TrueMark™ SARS-CoV-2, Flu A, Flu B, RSV Select Panel, and BioFire® RP2.1 Panel. The positive and negative predictive value of each test was evaluated at a 95% confidence interval. Results: Among the 200 tested samples, the TrueMark™ and OpenArray® laboratory-developed tests (LDTs) showed a 100% concordance for the detection of SARS-CoV-2, Flu A, Flu B, and RSV. Overall agreement of 100% was observed for nasopharyngeal samples between the laboratory-developed tests and FDA-approved BioFire® RP2.1 Panel. Diagnostic results for these four respiratory viruses, in clinical samples, between the LDTs and the FDA-approved comparator demonstrated full concordance. Conclusions: Respiratory viral infections represent one of the major global healthcare burdens. Consequently, the accurate detection and surveillance of these viruses are critical, particularly when these viruses are known to co-circulate. The excellent performance and full concordance of the LDTs, with the BioFire® Respiratory RP2.1 panel, in detecting SARS-CoV-2, Flu A, Flu B, and RSV shows that these tests can be confidently implemented for the clinical testing of respiratory viral infections.

Abstract Purpose: To present a rare case of metastatic conjunctival adenosquamous carcinoma (ASC) in the context of limited literature on the prognosis of ASC and suggested follow-up and surveillance. Case Report: We report a case of conjunctival ASC that metastasized to cervical lymph nodes five years after histological confirmation of complete local excision. Conclusion: Long-term clinical follow-up and surveillance imaging are warranted to allow early detection of disease recurrence and/or metastasis.

Abstract Microbial production of succinic acid (SA) at an industrially relevant scale has been hindered by high downstream processing costs arising from neutral pH fermentation for over three decades. Here, we metabolically engineer the acid-tolerant yeast Issatchenkia orientalis for SA production, attaining the highest titers in sugar-based media at low pH (pH 3) in fed-batch fermentations, i.e. 109.5 g/L in minimal medium and 104.6 g/L in sugarcane juice medium. We further perform batch fermentation using sugarcane juice medium in a pilot-scale fermenter (300×) and achieve 63.1 g/L of SA, which can be directly crystallized with a yield of 64.0%. Finally, we simulate an end-to-end low-pH SA production pipeline, and techno-economic analysis and life cycle assessment indicate our process is financially viable and can reduce greenhouse gas emissions by 34–90% relative to fossil-based production processes. We expect I. orientalis can serve as a general industrial platform for production of organic acids.

Abstract The carbon neutral potential of bioenergy relies in part on the ability of feedstocks to sequester carbon (C) in the soil. Sugarcane is one of the most widely used bioenergy crops, yet there remain unknowns about how it impacts soil C dynamics. In addition, Oilcane, a genetically modified version of Sugarcane has been produced to accumulate more energy‐dense oils and less soluble lignin, which enhances conversion efficiency but may also impact soil C cycling. Thus, our objectives were to examine the impact of Sugarcane litter decomposition on soil C formation and losses and determine if the genetic modifications to produce Oilcane alter these dynamics. To do this, we incubated bagasse (processed stem litter) and leaf litter from Sugarcane and Oilcane in microcosms with forest soil for 11 weeks. We used differences in natural abundance δ13C between C3 forest soil and C4 litter to trace the fate of the litter into respiratory losses as well as stable and unstable soil C pools. Our results show that genetic modifications to Oilcane did not substantially alter soil C dynamics. Sugarcane and Oilcane litter both led to net soil C gains dominated by an accumulation of the added litter as unstable, particulate organic C (POC). Oilcane litter led to small but significantly greater net soil C gains than Sugarcane litter due to greater POC formation, but the formation of stable, mineral associated organic matter (MAOC) did not differ between crop types. Sugarcane and Oilcane had opposing effects on tissue type where Sugarcane bagasse formed more MAOC, while Oilcane leaves preferentially remained as POC which may have important management implications. These results suggest that genetic modifications to Sugarcane will not significantly impact soil C dynamics; however, this may not be universal to other crops particularly if modifications lead to greater differences in litter chemistry.

Permeability-glycoprotein (P-gp) belongs to the ABS transporter protein family, with a high expression rate in cancerous cells. The substrate/inhibitors of the protein are structurally diverse, with no lucid mechanism of inhibition. There are two schools of thought on the inhibition mechanism: (i) P-gp inhibitors bind to the huge hydrophobic cavity between two Trans-Membrane Domains (TMDs), supported by ample literary proof and (ii) P-gp inhibitors bind to the vicinity of Nucleotide-Binding Sites (NBSs). Structural biologists have presented several experimental and theoretical structures of P-gp with bound nucleotides and inhibitors to explain the same. However, the available experimental P-gp structures are insufficient to address the catalytic transition path of mammalian P-gp in detail, thus the dynamics and mechanism by which drugs are effluxed is still unknown. Targeted Molecular Dynamics (targeted MD) could be used to minutely analyse and explore the catalytic transition inward open (IO) to outward open (OO) and relaxation path (OO to IO). Finally, analysis of targeted MD trajectory may help to explore different conformational states of Pg-p (reaction coordinate of catalytic transition/relaxation), efflux of compounds aided by the dynamics of Nucleotide Binding Domains/NBDs (ATP coupled process) and TMDs (peristalsis-like movement pushes the bound molecule). This review presents an understanding of the catalytic transition and dynamics of protein which provides insights at the efflux of chemotherapeutic drug using in cancer treatment.

Introduction Despite the high number of operations and surgical advancement, rehabilitation after rotator cuff repair has not progressed for over 20 years. The traditional cautious approach might be contributing to suboptimal outcomes. Our aim is to assess whether individualised (early) patient-directed rehabilitation results in less shoulder pain and disability at 12 weeks after surgical repair of full-thickness tears of the rotator cuff compared with current standard (delayed) rehabilitation.Methods and analysis The rehabilitation after rotator cuff repair (RaCeR 2) study is a pragmatic multicentre, open-label, randomised controlled trial with internal pilot phase. It has a parallel group design with 1:1 allocation ratio, full health economic evaluation and quintet recruitment intervention. Adults awaiting arthroscopic surgical repair of a full-thickness tear are eligible to participate. On completion of surgery, 638 participants will be randomised. The intervention (individualised early patient-directed rehabilitation) includes advice to the patient to remove their sling as soon as they feel able, gradually begin using their arm as they feel able and a specific exercise programme. Sling removal and movement is progressed by the patient over time according to agreed goals and within their own pain and tolerance. The comparator (standard rehabilitation) includes advice to the patient to wear the sling for at least 4 weeks and only to remove while eating, washing, dressing or performing specific exercises. Progression is according to specific timeframes rather than as the patient feels able. The primary outcome measure is the Shoulder Pain and Disability Index total score at 12-week postrandomisation. The trial timeline is 56 months in total, from September 2022.Trial registration number ISRCTN11499185.

Abstract Rare‐earth complexes are vital for separation chemistry and useful in many advanced applications including emission and energy upconversion. Here, 2D rare‐earth clusters having net charges are formed on a metal surface, enabling investigations of their structural and electronic properties on a one‐cluster‐at‐a‐time basis using scanning tunneling microscopy. While these ionic complexes are highly mobile on the surface at ≈100 K, their mobility is greatly reduced at 5 K and reveals stable and self‐limiting clusters. In each cluster, a pair of charged rare‐earth complexes formed by electrostatic and dispersive interactions act as a basic unit, and the clusters are chiral. Unlike other non‐ionic molecular clusters formed on the surfaces, these rare‐earth clusters show mechanical stability. Moreover, their high mobility on the surface suggests that they are in a 2D liquid‐like state.

Purpose: This paper examines the use of Artificial Intelligence/Machine Learning to extract a more comprehensive data set from a structured “standardized” form used to document Japanese American incarcerees during World War II.Setting/Participants/Resources: The Bancroft Library partnered with Densho, a community memory organization, and Doxie.AI to complete this work. Brief Description: The project digitized the complete set of Form WRA-26 “individual records'' for more than 110,000 Japanese Americans incarcerated in War Relocation Authority camps during WWII. The library utilized AI/machine learning to automate text extraction from over 220,000 images of a structured “standardized” form; our goal was to improve upon and collect information not previously recorded in the Japanese American Internee Data file held by the National Archives and Records Administration. The project team worked with technical, academic, legal, and community partners to address ethical and logistical issues raised by the data extraction process, and to assess appropriate access options for the dataset(s) and digitized records. Results/Outcome: Using AI/machine learning increased the quality of the data extracted from the digitized WWII era forms. Evaluation Method: A comparison of the earlier dataset extracted from the 1940s’s computer punch cards to the current data set extracted using AI/machine learning, the use of AI/machine learning showed marked improvement.

Sustainable management of municipal solid waste (MSW) is the utmost importance not only because of the health and environmental concerns but also due to its disposal issues of large quantities of waste generated and to achieve the Sustainable Development Goals (SDGs). Improper management of MSW causes hazards to inhabitants. Environmental and economic implications linked with the proper eco-friendly disposal of modern-day waste, has made it essential to come up with alternative waste management practices. Several studies revealed that approximately 90% of MSW disposed of unscientific manner as open dumps and landfilling, and created severe enigma to human health and the environment as well as contaminating the food chain cycle. It has been observed that urban local bodies (ULBs) in India have a big challenge in handling huge quantities of MSW; due to high density of population and insufficient infrastructure. Door to door collection of waste, methodologies for recycling MSW, and scientific treatments are some of the challenges. Considering these facts, the Union Ministry of Environment, Forests and Climate Change (MoEF&CC) India notified the new Solid Waste Management Rules (SWM), 2016 which would be revamped solid waste management in the country. Several steps of waste management/treatments are being adopted, i.e., incineration, pyrolysis, bio-refining and biogas plants, recycling and composting, composting is a sustainable low-cost option for MSW management, however, very less amount 6–7% of MSW was recycled through it. The present study emphasized a comprehensive review of the characteristics, production, collection, disposal and effective treatment technologies of MSW practiced in India.

18F-fluorodeoxyglucose Positron emission tomography (18F-FDG PET/CT ) is now being used as a single modality for metastatic workup and response evaluation in breast cancer. An increase in metabolic activity indicates disease progression; however, metabolic flare should be kept in mind. Metabolic flare is a well-documented phenomenon reported in metastatic breast and prostate cancer. Despite a favorable response to therapy, there is a paradoxical increase in radiopharmaceutical uptake. The flare phenomenon with various chemotherapeutic and hormonal agents is well acknowledged in bone scintigraphy. However, very few cases have been documented on PET/CT. Increased uptake may be noted after treatment is instituted. The increased osteoblastic activity is associated with the healing response of bone tumors. We report a case of treated breast cancer. She presented with metastatic recurrence after four years of initial management. The patient was started on paclitaxel chemotherapy. Serial 18F- FDG PET/CT demonstrated metabolic flare and complete metabolic response.

A Friedmann–Lemaitre–Robertson–Walker space–time model with all curvatures k=0, ±1 is explored in f(R,T) gravity, where R is the Ricci scalar, and T is the trace of the energy–momentum tensor. The solutions are obtained via the parametrization of the scale factor that leads to a model transiting from a decelerated universe to an accelerating one. The physical features of the model are discussed and analyzed in detail. The study shows that f(R,T) gravity can be a good alternative to the hypothetical candidates of dark energy to describe the present accelerating expansion of the universe.

Hospital-acquired infections (HAIs) pose a significant risk to global health, impacting millions of individuals globally. These infections have increased rates of morbidity and mortality due to the prevalence of widespread antimicrobial resistance (AMR). Graphene-based nanoparticles (GBNs) are known to possess extensive antimicrobial properties by inflicting damage to the cell membrane, suppressing virulence, and inhibiting microbial biofilms. Developing alternative therapies for HAIs and addressing AMR can be made easier and more affordable by combining nanoparticles with medicinal plants harboring antimicrobial properties. Hence, this study was undertaken to develop a novel graphene–silver nanocomposite via green synthesis using Trillium govanianum plant extract as a reducing agent. The resulting nanocomposite comprised silver nanoparticles embedded in graphene sheets. The antibacterial and antifungal properties of graphene–silver nanocomposites were investigated against several nosocomial pathogens, namely, Candida auris, Candida glabrata, Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, and Pseudomonas aeruginosa. The nanocomposite displayed broad-range antimicrobial potential against the test pathogens, with minimum inhibitory concentrations (MICs) ranging between 31.25 and 125.0 µg/mL, and biofilm inhibition up to 80–96%. Moreover, nanocomposite-functionalized urinary catheters demonstrated hemocompatibility towards sheep erythrocytes and imparted anti-fouling activity to the biomaterial, while also displaying biocompatibility towards HEK 293 cells. Collectively, this investigation highlights the possible application of green-synthesized GBNs as an effective alternative to conventional antibiotics for combating multidrug-resistant pathogens.

The review paper comprised the impact of geographical and environmental factors on polyploidy and vice versa. The review covers different effects of geographical factors, like spatial isolation, altitude, and local climate on polyploidy, and the behavior of polyploid(s) in abiotic factors, such as temperature and light with a few examples of northwest Himalayas. The paper concludes that polyploid plants behave differently in environmental conditions, as polyploids are more prominent in higher altitudes, colder environments, and nutrient-rich soil than diploid progenitors, but have a mixed distribution in different geographical conditions. Further, polyploidy is more common among perennials than annuals, while niche differentiation depends more on the local environment. The virtual case study results from North and North Western India have been shown with the help of ArcGIS online software. The scrutiny of spatial distribution on maps highlights the fact that polyploidy is still a complex research puzzle with interesting perspectives.

Abstract Biofilms are complex, three-dimensional structures that provide a long-established survival mechanism for microorganisms. Biofilms play a substantial role in pathogenesis as they can evade the immune response and be highly resistant to conventional antimicrobials, thus impacting the human health and healthcare system. To address this issue, BMC Microbiology invites submissions to the collection ‘Biofilms and its impact on disease’.

Context: Nowadays, dentists use tooth-colored materials extensively, but the bond strength of these materials with teeth is crucial to determine their long-span durability. As a result, the bond strength of these materials must be assessed. Aims: This study aimed to compare the push-out bond strength of alkasite restorative material and bulk-fill composite when used with a universal adhesive system. Settings and Design: In vitro study to determine the push-out bond strength with a universal testing machine. Subjects and Methods: Forty extracted mandibular molars were taken and their occlusal surface was wet grounded with silicon carbide paper so that a smooth dentin surface was obtained. Class I cavities were prepared with a template to standardize the size of the preparation. Teeth were divided into Group I and Group II of 20 teeth each. Alkasite restorative material was used in Group I, and nanohybrid bulk-fill composite was used in Group II. Restored samples were sliced into 2 mm thick sections and examined for push-out bond strength. Results: Push-out bond strength of Cention-N was 201.25 and the composite was 163.69, and the results were statistically significant (P = 0.017). Statistical Analysis Used: Unpaired t-test. Conclusions: It was concluded that alkasite material had more push-out bond strength than bulk-fill composite when used with a universal adhesive system.

Spine gourd (SG) is a nutritionally and medicinally rich seasonal vegetable grown in tropical areas. Therefore, the present study accessed the effect of drying temperature (40, 50, 60, and 70 °C) on chlorophyll, ascorbic acid, proximate, antioxidant, and minerals contents of ultrasound (US) pretreated SG. The increase in drying temperature (40−70 °C) after pretreatment increased the total flavonoids, fiber, protein and ash content compared to freeze-dried (control) sample. In contrast, elevated drying temperature significantly degraded the chlorophyll, ascorbic acid, carbohydrates, fat, total phenolic, and DPPH activity. In addition, the effect of drying temperature on the mineral contents was comparable and a significant variation was observed between control and US pretreated samples. The FTIR spectra revealed that functional group integrity was preserved even at higher drying temperatures. Conclusively, the US pretreated sample dried at 40 °C showed a better nutritional content (comparable to freeze-dried samples) than those dried at higher temperatures. However, samples dried at 70 °C were rich in protein and antioxidants.

Rare-earth elements are vital to advanced technological applications ranging from spintronic devices to quantum information science. Here, the authors formed charged rare-earth complexes on a material surface and demonstrated atomically precise control on their rotational dynamics.

Study Design A prospective comparative study. Purpose To compare the incidence of unintended durotomy and return to work after open surgery versus minimally invasive spine surgery (MIS) for degenerative lumbar pathologies. Overview of Literature The incidence of accidental durotomy varies between 0.3% and 35%. Most of these are from open surgeries, and only a handful of studies have involved the MIS approach. No single-center studies have compared open surgery with MIS, especially in the context of early return to work and dural tear (DT). Methods This study included 420 operated cases of degenerative lumbar pathology with a prospective follow-up of at least 6 months. Patients were divided into the open surgery and MIS groups, and the incidences of DT, early return to work, and various demographic and operative factors were compared. Results A total of 156 and 264 patients underwent MIS and open surgery, respectively. Incidental durotomy was documented in 52 cases (12.4%); this was significantly less in the MIS group versus the open surgery group (6.4% vs. 15.9%, p

Acetyl-CoA carboxylase (ACCase: EC 6.4.1.2) is one of the essential biotins containing enzymes required by plants for fatty acid synthesis and elongation. The unique enzyme is present in its homomeric form in all the Gramineae family, making it a suitable target for developing herbicides selectively against weeds of the Gramineae family. One such example is infestation of Phalaris minor in winter wheat crop fields, where aryloxyphenoxypropionates (FOP); cyclohexanediones (DIM) and phenyl pyrazoline (DEN) group of ACCase inhibiting herbicides are used. However, the increasing number of ACCase herbicide resistant weed populations has compelled agro-scientists to seek varied possibilities for weed control, through Integrated Weed Management (IWM) strategies. Developing new potential herbicides to regain sensitivity in weeds could be an approach to weed control. The current advancement in computational techniques could be of aid in developing new herbicide-like molecules by exploring the genomics, proteomics and structural details of catalytic sites of herbicide action in crops as well as weeds.

Whole and fresh-cut produce are minimally processed and susceptible to microbial contamination. This study evaluated the survival or growth of L. monocytogenes on peeled rinds, and fresh-cut produce at different storage temperatures. Fresh-cut fruits and vegetables, including cantaloupe, watermelon, pear, papaya, pineapple, broccoli, cauliflower, lettuce, bell pepper, and kale (25 g pieces) were spot inoculated with 4 log CFU/g of L. monocytogenes and stored at 4 or 13°C for 6 days. Cantaloupe and bell pepper rind disks (20 cm2), mimicking whole produce were inoculated with low inoculum level (4 log CFU/mL) and high inoculum level (6 log CFU/mL) and stored at 24°C up to 8 days and 4°C up to 14 days, respectively. L. monocytogenes counts on fresh-cut pear samples stored at 4°C increased significantly by 0.27 log CFU/g. However, Listeria levels on kale (day 4), cauliflower (day 6), and broccoli (day 2) were significantly reduced by 0.73, 1.18, and 0.80 log CFU/g, respectively, at 4°C. At 13°C, the bacterial counts increased significantly after a day of storage on fresh-cut watermelons (increasing by 1.10 log CFU/g) and cantaloupes (increasing by 1.52 log CFU/g). Similar increases were observed on pears (1.00 log CFU/g), papayas (1.65 log CFU/g), and green bell peppers (1.72 log CFU/g). Pineapple samples did not support the growth of L. monocytogenes at 13°C with a significant reduction of 1.80 log CFU/g by day 6. L. monocytogenes levels significantly increased in fresh-cut lettuce at 13°C but remained stable on kale, cauliflower, and broccoli after 6 days of storage. Stable population was observed also on cantaloupe rinds up to 8 days at 24°C. While on the outer surface of bell peppers, the population level decreased below the detectable limit of the test (10 CFU/20 cm2) after 14 days of storage at 4°C. The results demonstrated variable survival behavior of L. monocytogenes on fresh-cut produce with produce type and storage temperature.

As an emerging economy, India’s post-pandemic resilience supports this study. We scrutinize current MD&A (Management Discussion and Analysis) reporting practices by Indian firms, aligning with the 'Industry, Innovation, and Infrastructure Development' goal of Sustainability. Data was extracted from annual reports of selected listed Indian companies for the years 2016-2021. The data was analyzed using content analysis and t-tests. We found an encouraging increase in the quantity and quality of disclosures over time, even during the pandemic. However, there is a significant discrepancy with quality lagging behind quantity. These findings highlight an urgent need for improved MD&A disclosures. Managers, regulators, and organizations could use this study to identify issues and strategically direct their efforts for maximum benefits in this domain. This pioneering study assesses adherence to MD&A practices within the Indian context, extending its contribution to the accounting literature of other developing Asian economies, such as Indonesia, Malaysia, and China, where MD&A practices are still evolving.

Research purpose. This study aimed to determine the EMG analysis of forehand topspin using different ball materials. Materials and methods. For the purposes of this study, seven male university-level table tennis players who were right-hand dominant players (age 21.1 ± 2.4 years, height 162.6 ± 6.8 cm, mass 61.8 ± 3.2 kg) with at least 6 years of playing experience were selected. The selected participants were physically active and had no physical injuries. The study was approved by the research committee. Pectoralis Major (PM), Deltoid Anterior (DA), Latissimus Dorsi (LD), Biceps Brachii (BB), Extensor Carpi Radialis (ECR), Flexor Carpi Radialis (FCR) muscles were selected for the electromyographical study. One of the EMG signals variable Root Mean Square was measured in all muscles, through which the maximum muscle involvement was evaluated. For each assessed muscle and both ball materials, the mean value of Root Mean Square EMG signals was computed. For descriptive statistics, minimum, maximum, mean, and standard deviation were used, and a t-test was used as a statistical technique. Results. The level of significance was set at 0.05. Results of the study showed no significant difference in EMG variable Root Mean Square between celluloid and poly plastic ball material on selected muscles. Conclusions. We conclude that the biceps brachii and extensor carpi radialis are important muscles for the forehand topspin because they are responsible for flexion of the arm and work strongly in the action of gripping in the performance of quick forehand spin strokes.

Background and aim: Steroid drops are necessarily used after cataract surgery to control the ocular inflammation, but they can increase the intraocular pressure. Using two different steroid eye drops as post-operative medication after cataract surgery to compare the intraocular pressure variation and control of ocular inflammation. Material and methods: A comparative hospital-based study was conducted on 180 patients who underwent cataract surgery with Intraocular lens implantation. Two groups 1 and 2 were made based on the exposure to either Dexamethasone 0.1% e/d or Prednisolone acetate 1% e/d, respectively. Both drops were prescribed six times daily for the first week and gradually tapered off in 6 weeks. The anti-inflammatory effect and mean IOP rise from baseline in groups 1 and 2 were analyzed and compared. Statistical analysis was done using SPSS 24.0. Results: Intraocular Pressure increase was noted more in group 1 (25.56%) versus group 2 (17.78%), with a statistically significant difference (p

Depending on the type, drought events are described using different indices, such as meteorological, agricultural, and hydrological. The use of different indices often causes confusion for making water-related management decisions. One simple summarized index which can describe the different aspects of drought is desired. Several methods have therefore been proposed, especially with the linear combination method which does not adequately describe drought characteristics. Meanwhile, autoencoders, nonlinear transformation in dimensional reduction, have been applied in the deep learning literature. The objective of this study, therefore, was to derive autoencoder-based composite drought indices (ACDIs). First, a basic autoencoder was directly applied as ACDI, illustrating a negative relation with the observed drought indices which was further multiplied by a negative. Also, the hyperbolic tangent function was adopted instead of the sigmoid transfer function due to its higher sensitivity to drought conditions. For better expression of drought indices, positive and unity constraints were applied for weights, denoted as ACDI-C. Further simplification was made as sACDI by excluding the decoding module since it was not necessary. All applied weights of different sites over a country can be unified into one weight, and the same weights were made for all the sites, called as sACDI1. In the context of model evaluation, a comprehensive analysis was undertaken employing metrics as root mean squared error (RMSE), mean absolute error (MAE), and correlation coefficients. The collective findings underscore the superior performance of both the sACDI and sACDI1 models over their counterparts. Notably, these simplified models manifestly diminished RMSE and MAE values, indicating their enhanced predictive capabilities. Of particular note, sACDI1 exhibited a discernibly lower MAE in comparison to alternative models. Further alarm performance metrics was conducted including the false alarm ratio, probability of detection, and accuracy (ACC). The investigations revealed superiority of the simplified models in terms of alarm ACC, especially in the case of SRSI(A). The developed ACDI can comprehensively summarize multiple drought aspects and provide summarized information about drought conditions.

Breast cancer is the most commonly diagnosed cancer and a leading cause of death in women worldwide. It is a heterogeneous disease, as shown by the gene expression profiles of breast cancer samples. It begins in milk-producing ducts, with a high degree of diversity between and within tumors, as well as among cancer-bearing individuals. The enhanced prevalence of breast cancer is influenced by various hormonal, lifestyle, and environmental factors, and very early onset of the disease correlates strongly with the risk of local and distant recurrence. Many subtypes are difficult to treat with conventional therapeutic modalities, and therefore, optimal management and early diagnosis are the first steps to minimizing the mortality linked with breast cancer. The use of newer methods of nanotechnology extends beyond the concept of synthesizing drug delivery mechanisms into the creation of new therapeutics, such as delivering chemotherapeutics with nanomaterial properties. Exosomes, a class of nanovesicles, are emerging as novel tools for deciphering the patient-specific proteins and biomarkers across different disease models, including breast cancer. In this review, we address the role of exosomal miRNA in breast cancer diagnosis and treatment.

The tobacco cutworm, Spodoptera litura (Fabricius) is a serious cosmopolitan pest that attacks several economically important crops such as maize, sorghum, chickpea, pigeon pea, cotton, tobacco and sunflower. It has developed resistance to most pesticides resulting in its continual outbreak. The effect of caffeic acid on second instar larvae of S. litura was evaluated by carrying out bioassays, nutritional assays, immune assays and biochemical assays with phenolic acids. Bioassays carried out with second instar larvae of S. litura showed growth inhibiting effects of various concentrations (5 ppm, 25 ppm, 125 ppm, 625 ppm and 3125 ppm) of caffeic acid on S. litura in comparison to control. A significant increase in mortality as well as an increased development time was observed with increase in the concentration of caffeic acid. A decrease in nutritional indices, including relative growth rate (RGR), relative consumption rate (RCR), efficiency of conversion of ingested food (ECI), efficiency of conversion of digested food (ECD), and approximate digestibility (AD), indicated that dietary caffeic acid also negatively impacted the nutritional physiology of S. litura larvae. Caffeic acid has a significant impact on the immunological response of S. litura larvae. As the concentration of caffeic acid increased, the overall number of hemocytes decreased. Enzymatic assays revealed a significant increase in antioxidant enzymes when S. litura larvae were given an artificial diet containing LC50 concentration of phenolic acid for an interval of 24, 48, 72 and 96 h. The levels of oxidative stress markers (hydrogen peroxide, protein carbonyl and lipid peroxide) were also significantly enhanced in S. litura larvae after treatment with phenolic acid. According to our study, caffeic acid can be employed as a substitute for traditional insecticides to reduce the population of S. litura.

The first teeth to erupt in the oral cavity are the mandibular molars which are more prone to endodontic and periodontal involvement. This may lead to significant issues like an increase in pocket depth, furcation involvement, loss of clinical attachment and mobility of teeth ultimately resulting in tooth loss. Thus, it necessitates cautious oral hygiene measures. Advances in dentistry have led to treatment modalities for preserving teeth which were earlier indicated for extraction. Hence, periodontally compromised tooth with severe furcation involvement, which may not be amenable for regeneration, may be retained by bicuspidisation. Bicuspidisation is a surgical technique which involves separation of mesial and distal roots of mandibular molars along with its crown portion, where both segments are then retained individually, followed by rehabilitation with fixed prosthesis of the individual segments. This not only eliminates furcation involvement but also facilitates effective oral hygiene maintenance. In the present case report, a 50-year-old male patient reported with the chief complaint of pain, sensitivity and food lodgement in left mandibular first molar. Clinical and radiographic examination revealed grade III furcation involvement, significant pocket depths, delayed electric pulp test response and bone loss in furcation area. Bicuspidisation was opted as the suitable treatment modality. Root canal treatment was done, followed by bicuspidisation and subsequent rehabilitation with novel prosthetic technique. The bicuspids were restored using combination of deep margin elevation, and non splinted Lithium Disilicate table-top restorations. Six month follow-up showed good healing of hard and soft tissues around the bicuspidized sections, which was indicative of a successful treatment outcome, and can be attributed to a careful case selection, choice of restorative materials and techniques as well as good oral hygiene compliance.