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Objective: To evaluate effect of iodized oil in crossbred cattle suffering from hyperthermia (heat intolerance syndrome) in Punjab, India. Methods: The present study was conducted in 85 clinical cases of summer hyperthermia in exotic and crossbred cows with rectal temperature of ≥40.56 °C. Haematological examinations of the affected cows (55) showed relatively low haemoglobin, normal differential and total leucocytic count. All the affected cows (85) were treated with three doses of iodized oil (750 mg elemental iodine per dose, 5mL) given at 24 h interval by subcutaneous route in brisket region. Results: Seventy seven (90.58%) cases showed remarkable clinical improvement with temperature coming to normal (38.33 to 38.88 °C) within 5 d of start of treatment. The appetite and milk production was restored by 10th day post treatment. Conclusions: The findings of this study substantiate that subcutaneous administration of three doses of iodized oil given at 24 h interval is effective in decreasing rectal temperature of hyperthermic cattle.