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Programmed death-ligand 1 (PD-L1) expression has been approved as an immune checkpoint inhibitor (ICI) response predictive biomarker; however, the clinicopathological and molecular features of HPV-positive oropharyngeal squamous cell carcinoma [HPV(+)OPSCC] based on PD-L1 expression are not well studied. We aimed to characterize clinicopathological, tumor immune microenvironmental, and molecular features of HPV(+)OPSCC with different PD-L1 expression scored by combined positive score (CPS). A total of 112 cases were collected from 2008-2021 and received PD-L1 and CD8 immunohistochemistry (IHC) staining. 71 samples received DNA sequencing out of which 32 samples received RNA sequencing for immune-related gene alterations or expression analysis. The 32 samples were also subjected to analysis of CD20, CD4, CD8, CD68, Foxp3 and P16 by multiplex immunofluorescence (mIF) staining, and the immune markers were evaluated in the tumor body (TB), tumor margin (TM) and normal stroma (NS) regions separately. Our results showed that of 112 HPV(+)OPSCC tumors, high(CPS≥20), intermediate(1≤CPS

Objective. To evaluate diagnostic utility of Dishevelled-3 (DVL-3) mRNA and δ-catenin mRNA expression in pleural effusions of patients with lung cancer. Methods. DVL-3 mRNA and δ-catenin mRNA levels were assessed by performing RT-PCR on pleural effusion specimens from patients with lung cancer (n=75) and with lung benign disease (n=51). Results. The expressions of DVL-3 mRNA and δ-catenin mRNA were significantly higher in malignant than in benign lung disease (P

A highly transparent and super-hydrophilic double-layer anti-reflection coating was prepared by a simple and economical sol-gel method based on the glass substrate. The bottom and top layer were respectively made with SiO _2 –TiO _2 sol and SiO _2 –TiO _2 -PEG300 sol. The average transmittance of the substrate coated with the double-layer coating is increased by 7% up to 97.4% in the visible light band, and the peak transmittance reaches 99% at about 550 nm. In addition, double-layer anti-reflection coating shows a good super-hydrophilic self-cleaning performance, and the transmittance of the coating can be restored by simple washing with water. Furthermore, due to the existence of anatase TiO _2 , the composite coating can restore the super-hydrophilicity damaged by dust under UV light.