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Thoth is a free desktop/laptop software application with a friendly graphical user interface that facilitates routine data-visualization and statistical-calculation tasks for astronomy and astrophysical research (and other fields where numbers are visualized). This software has been upgraded with many significant improvements and new capabilities. The major upgrades consist of: (1) six new graph types, including 3D stacked-bar charts and 3D surface plots, made by the Orson 3D Charts library; (2) new saving and loading of graph settings; (3) a new batch-mode or command-line operation; (4) new graph-data annotation functions; (5) new options for data-file importation; and (6) a new built-in FITS-image viewer. There is now the requirement that Thoth be run under Java 1.8 or higher. Many other miscellaneous minor upgrades and bug fixes have also been made to Thoth. The newly implemented plotting options generally make possible graph construction and reuse with relative ease, without resorting to writing computer code. The illustrative astronomy case study of this paper demonstrates one of the many ways the software can be utilized. These new software features and refinements help make astronomers more efficient in their work of elucidating data.

We complete the analysis of planetary candidates found by the KMT AnomalyFinder for the 2017 prime fields that cover ∼13 deg ^2 . We report three unambiguous planets: OGLE-2017-BLG-0640, OGLE-2017-BLG-1275, and OGLE-2017-BLG-1237. The first two of these were not previously identified, while the last was not previously published due to technical complications induced by a nearby variable. We further report that a fourth anomalous event, the previously recognized OGLE-2017-BLG-1777, is very likely to be planetary, although its light curve requires unusually complex modeling because the lens and source both have orbiting companions. One of the three unambiguous planets, OGLE-2017-BLG-1275, is the first AnomalyFinder discovery that has a Spitzer microlens parallax measurement, π _E ≃ 0.045 ± 0.015, implying that this planetary system almost certainly lies in the Galactic bulge. In the order listed, the four planetary events have planet-host mass ratios q and normalized projected separations s of $(\mathrm{log}q,s)=(-2.31,0.61)$ , (−2.06, 0.63/1.09), (−2.10, 1.04), and (−2.86, 0.72). Combined with previously published events, the 2017 prime fields contain 11 unambiguous planets with well-measured q and one very likely candidate, of which three are AnomalyFinder discoveries. In addition to these 12, there are three other unambiguous planets with large uncertainties in q .

Dysregulation of neurotransmission is a feature of several prevalent lower urinary tract conditions, but the mechanisms regulating neurotransmitter release in the bladder are not completely understood. The unconventional motor protein, Myosin 5a, transports neurotransmitter-containing synaptic vesicles along actin fibers towards the varicosity membrane, tethering them at the active zone prior to reception of a nerve impulse. Our previous studies indicated that Myosin 5a is expressed and functionally relevant in the peripheral nerves of visceral organs such as the stomach and the corpora cavernosa. However, its potential role in bladder neurotransmission has not previously been investigated. The expression of Myosin 5a was examined by quantitative PCR and restriction analyses in bladders from DBA (dilute-brown-nonagouti) mice which express a Myosin 5a splicing defect and in control mice expressing the wild-type Myosin 5a allele. Functional differences in contractile responses to intramural nerve stimulation were examined by ex vivo isometric tension analysis. Data demonstrated Myosin 5a localized in cholinergic nerve fibers in the bladder and identified several Myosin 5a splice variants in the detrusor. Full-length Myosin 5a transcripts were less abundant and the expression of splice variants was altered in DBA bladders compared to control bladders. Moreover, attenuation of neurally-mediated contractile responses in DBA bladders compared to control bladders indicates that Myosin 5a facilitates excitatory neurotransmission in the bladder. Therefore, the array of Myosin 5a splice variants expressed, and the abundance of each, may be critical parameters for efficient synaptic vesicle transport and neurotransmission in the urinary bladder.

ABSTRACT IMPACT: Demonstrate the role of astrocyte released MMPs in response to pathogenic HIV protein Tat. OBJECTIVES/GOALS: In the presence of the pathogenic HIV protein Tat, astrocytes have been demonstrated to adopt an inflammatory phenotype as well as release extracellular matrix degrading enzymes, MMPs. Our work aims to identify whether MMPs alter perineuronal net integrity and working memory in a mouse model of Tat-induced neuroinflammation. METHODS/STUDY POPULATION: Stereotaxic Injection: C57BL6/J mice were injected bilaterally with HIV-1 IIIB Tat 5ug in 5uL or Vehicle (0.2M KCl, 5mM DTT, 50mM Tris, pH 8.0), into the hippocampus (CA1; -1.9mm AP, ±1.6mm ML, -1.5mm DV from pial surface). All outcome measurements were performed 14-days post injection. Behavior: T-maze was used to assess working memory following Tat exposure. qRT-PCR: TaqMan probes were used according to manufacturer on extracted whole hippocampus mRNA. IF: GFAP and CD68 immunofluorescence was used to determine inflammation post injection. Inhibitory interneurons (parvalbumin positive) and peri-neuronal nets (WFA positive) were quantified. WB: Synaptosomes from whole hippocampi (Syn-PER) were isolated and synaptic excitatory markers were quantified (PSD-95, synaptophysin, GluR2a). RESULTS/ANTICIPATED RESULTS: Tat exposure resulted in impairments in working memory as measured by T-maze alternations and an increase in hippocampal mRNA expression of MMP-13 and IL-1β, indicative of neuroinflammation. We also noted an increase in GFAP+ injection site width 14 days post-Tat injection, suggesting robust gliosis. While there were no changes in the excitatory pre and post synaptic markers we found a significant decrease in the percent of PV+ interneurons with peri-neuronal nets (PNNs) following Tat exposure. Taken together, this preliminary data supports a role for inflammation and PNN integrity in Tat-induced alterations in working memory. DISCUSSION/SIGNIFICANCE OF FINDINGS: Our findings suggest that Tat contributes to cognitive impairment and that astrogliosis with elevated MMP-13 facilitates the degradation of peri-neuronal nets (PNNs) within the hippocampus. Since PNN degradation can alter neuronal circuitry future studies will focus on Tat-induced changes in hippocampal signaling.

We have collected transit times for the TRAPPIST-1 system with the Spitzer Space Telescope over four years. We add to these ground-based, HST, and K2 transit-time measurements, and revisit an N -body dynamical analysis of the seven-planet system using our complete set of times from which we refine the mass ratios of the planets to the star. We next carry out a photodynamical analysis of the Spitzer light curves to derive the density of the host star and the planet densities. We find that all seven planets’ densities may be described with a single rocky mass–radius relation which is depleted in iron relative to Earth, with Fe 21 wt% versus 32 wt% for Earth, and otherwise Earth-like in composition. Alternatively, the planets may have an Earth-like composition but enhanced in light elements, such as a surface water layer or a core-free structure with oxidized iron in the mantle. We measure planet masses to a precision of 3%–5%, equivalent to a radial-velocity (RV) precision of 2.5 cm s ^−1 , or two orders of magnitude more precise than current RV capabilities. We find the eccentricities of the planets are very small, the orbits are extremely coplanar, and the system is stable on 10 Myr timescales. We find evidence of infrequent timing outliers, which we cannot explain with an eighth planet; we instead account for the outliers using a robust likelihood function. We forecast JWST timing observations and speculate on possible implications of the planet densities for the formation, migration, and evolution of the planet system.

Composite materials can enhance morphing and deployable structure capability due to their high degree of tailorability and their favourable stiffness- and strength-to-weight ratios. One such structure, the bistable helical lattice, is augmented in current work. To date this type of structure, shows promise in aerospace systems which require linear actuation. Herein, morphing capabilities are enhanced by removing traditional mechanical fasteners at the joints, and replacing them with magnets which allow detachment and re-attachment in a controlled, purposeful way. Within a helical lattice structure, joint detachment creates new functionality by allowing a new topology to be formed which is used to convert a linear actuator to one that is curved and then back again, when the joints are reattached. The required force to actuate the topological change is characterised through the use of both finite element analysis and experimental testing. The structural response is observed through the manufacture and testing of a demonstrator which replaces the traditional joints with a series of magnets in order to capture this variable topology behaviour.