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Abstract Background Seafood is increasingly traded worldwide, but its supply chain is particularly prone to frauds. To increase consumer confidence, prevent illegal trade, and provide independent validation for eco-labelling, accurate tools for seafood traceability are needed. Here we show that the use of microbiome profiling (MP) coupled with machine learning (ML) allows precise tracing the origin of Manila clams harvested in areas separated by small geographic distances. The study was designed to represent a real-world scenario. Clams were collected in different seasons across the most important production area in Europe (lagoons along the northern Adriatic coast) to cover the known seasonal variation in microbiome composition for the species. DNA extracted from samples underwent the same depuration process as commercial products (i.e. at least 12 h in open flow systems). Results Machine learning-based analysis of microbiome profiles was carried out using two completely independent sets of data (collected at the same locations but in different years), one for training the algorithm, and the other for testing its accuracy and assessing the temporal stability signal. Briefly, gills (GI) and digestive gland (DG) of clams were collected in summer and winter over two different years (i.e. from 2018 to 2020) in one banned area and four farming sites. 16S DNA metabarcoding was performed on clam tissues and the obtained amplicon sequence variants (ASVs) table was used as input for ML MP. The best-predicting performances were obtained using the combined information of GI and DG (consensus analysis), showing a Cohen K-score > 0.95 when the target was the classification of samples collected from the banned area and those harvested at farming sites. Classification of the four different farming areas showed slightly lower accuracy with a 0.76 score. Conclusions We show here that MP coupled with ML is an effective tool to trace the origin of shellfish products. The tool is extremely robust against seasonal and inter-annual variability, as well as product depuration, and is ready for implementation in routine assessment to prevent the trade of illegally harvested or mislabeled shellfish.

ABSTRACT Objective To investigate the prognostic significance of thyroid hormone profile in patients hospitalized for decompensated cirrhosis. Subjects and methods Prospective cohort study that included 119 subjects. All subjects were evaluated at admission and followed for 90 days. TSH, fT3, fT4 were measured within 24 hours of hospitalization. Results Higher fT4 and lower fT3 levels were observed among Child-Pugh C patients as compared to Child-Pugh A and B, and in those with acute-on-chronic liver failure (ACLF). Lower fT3/fT4 ratio was observed in those with ascites, infections, ACLF, and in Child-Pugh C. Ninety-day mortality was 26.9% and it was independently associated with higher Model for End-stage Liver Disease (MELD) and TSH, and lower fT3/fT4 ratio in multivariate analysis. A new prognostic model including MELD, TSH and fT3/fT4 ratio was devised. The areas under the receiver operating characteristic curves for MELD, fT3/fT4 ratio, TSH (μIU/mL), and the new model for predicting 90-day mortality were 0.847 ± 0.041, 0.841 ± 0.039, 0.658 ± 0.062, and 0.899 ± 0.031, respectively. The 90-day survival was 31.6% in patients with values of the predictive model ≥ -0.77 and 93.5% for values < -0.77 (P < 0.001). Conclusions Thyroid hormone profile was strongly associated with worse outcomes in patients with cirrhosis and might represent promising prognostic tools that can be incorporated in clinical practice.

Over recent decades, there was a substantial evolution in the productive management of dairy animals worldwide with a consequent boost in individual milk yield. This evolution positioned the milk production as the central metabolic priority around which all other physiological functions are coordinated and partially minimised. This shift underscores the crucial role of effectively managing stressful phases in intensive dairy farming systems, also highlighting the resilience exhibited by the animals. Indeed, monitoring the nutritional and health status becomes paramount, aiming for an early detection of (sub)clinical health impairments. Given the mammary gland’s centrality in high-yielding dairy breeds, it’s unsurprising that the milk matrix provides insights about udder itself but also systemic metabolic function. The emerging field of milk phenomics explores links between milk components and animal health, holding great promise for studying dairy cow resilience. The use of infrared spectroscopy on milk to predict indicators and complex traits at the herd level is a promising approach. In the dairy sector, the available infrared instruments mainly implement the Fourier transform infrared (FTIR) spectroscopy. This method is widely employed in milk recording schemes worldwide for animal monitoring and breeding purposes. In addition, visible and near-infrared (NIR) spectroscopy is increasingly integrated into milking systems for daily on-farm monitoring of milk quality and animals’ physiological status. This review examines the topic of milk phenomics together with potential and challenges of infrared spectroscopy to predict indicators and complex traits related to health and nutritional disorders exploiting the biological bonds that exist between milk and blood in dairy animals.HIGHLIGHTS Milk is a valuable source of information for assessing nutritional and health proxies in dairy animals. Infrared spectroscopy is used within routine milk recording systems for the determination of major components, but other useful applications exist. This review summarises research on milk phenomics carried out by mean of infrared spectroscopy to predict complex traits related to animals’ health and nutritional disorders.

AbstractOur study aimed to evaluate the effect of different treatments for BRD on health and welfare in fattening bulls. A total of 264 bulls were enrolled. Welfare was assessed on day 2 (T0) and day 15 (T1) after arrival. A decrease in the welfare level was observed from T0 to T1. All bulls were inspected clinically at T0 and T1 revealing an increase of skin lesions and lameness in T1. In both periods, a high incidence of respiratory disease was observed. A prevalence of 79.55% and 95.45% of Mycoplasma bovis using RT-PCR and culture at T0 and T1 respectively was observed. Blood samples were collected for haematology at T0 and T1. At T0, 36 animals were individually treated for BRD with an antimicrobial (IT), 54 received a metaphylactic treatment with tulathromycin (M), 150 received a metaphylactic treatment with tulathromycin plus a second antimicrobial (M + IT) whereas 24 were considered healthy and therefore not treated (NT). Additionally, 128 were treated with a non-steroid anti-inflammatory (NSAID). Neutrophils of M + IT were significantly higher than groups NT and M and the lymphocytes of M + IT were significantly lower than that of IT. White blood cells, neutrophils and N/L ratio of animals treated with an NSAID was significantly higher than that not treated. Lung inspection of 172 bulls at the abattoir indicated that 92.43% presented at least one lung lesion. A statistically significant effect of the NSAID treatment on the lung lesions was observed. Our findings indicate that BRD was a major welfare and health concern and evidence the difficulties of antimicrobial treatment of M. bovis.

This study aimed at evaluating the environmental impact (EI) of herds representative of the Italian heavy pig production system to investigate the EI variation associated with farm management (FM), diet formulation (DF) and animal response (AR) variables. Data originated from 8 breeding farms, which included the sows and preweaning piglets (Site1) and the post-weaned piglets (7.5–30 kg body weight – BW, Site2) and 10 growing–fattening farms, dealing with 30–170 kg BW pigs (Site3). A cradle-to-farm gate Life Cycle Assessment was used, with 1 kg BW gain (BWG) as functional unit. Impact categories were global warming (GWP), acidification (AP) and eutrophication (EP) potentials and land occupation (LO). Whole-production cycle EI (Site123, 0–170 kg BW) was obtained summing up EIs of 0–30 and 30–170 kg BW animals. Impacts were analysed with one- or two-way PERMANOVA to test the effect of FM, DF and AR variables. The Site123-related EI averaged nearly 3.1 kg CO2-eq (GWP), 50 g SO2-eq (AP), 22 g PO4-eq (EP) and 4.9 m2/y (LO) per kg BWG. Site3 contributed nearly 80% of the whole impact. Regarding Site1, GWP resulted mitigated by increasing sow productivity. Site2 EI resulted mitigated by decreasing feed conversion ratio and increasing average daily gain, whereas Site3 EI by increasing feed self-sufficiency and decreasing dietary crude protein and mortality. In perspective, given the relevance of Site3 on the whole-pig system EI, a deeper integration between the farm agronomical management and the growing–finishing pigs’ diets formulation would support the environmental sustainability of heavy pig operations.

Abstract Windows provide access to daylight and outdoor views, influencing building design. Various glazing and window shade materials are used to mitigate glare, overheating and privacy issues, and they affect view clarity. Among them, we evaluated the effect of window films, electrochromic (EC) glass, and fabric shades on view clarity. We conducted an experiment with 50 participants using visual tests adapted from clinical vision tests (visual acuity, contrast sensitivity, color sensitivity) and images displayed on a computer monitor in a controlled laboratory. Window films and EC glass tints outperformed fabric shades in visual acuity, contrast sensitivity and view satisfaction with the exception of the darkest EC tint state and dark grey VLT 3% shade for color sensitivity and view satisfaction. The EC tints pose internal reflection issues and fabric shades are preferred for visual privacy. Window films and EC glass hinder participants’ blue–green color discrimination while fabric shades also decrease red–yellow color discrimination. Visual acuity predicts view satisfaction and contrast sensitivity is the strongest predictor for visual privacy. Generally, higher visible light transmittance and lower solar reflectance (darker color) enhance human visual performance. The proposed workflow provides an experimental procedure, identifies the primary variables and establishes a predictive framework for assessing view clarity of fenestration.

Abstract Background The current Italian scenario of pediatric palliative care (PPC) services is characterized by inadequate coverage of the territory. Therefore, it is important to improve the referral of patients to the most appropriate setting (community care, general PPC, or specialized PPC) and to improve the delivery of PPC care. Methods Aiming at obtaining information about the referrals to the Padua Pediatric Hospice that could help estimate the investments needed to improve the provision of care, a retrospective analysis has been carried out. The rate of proper referral and discharge, the number of patients followed at the hospice, the mortality rate, and the length of follow-up were analyzed, and, when possible, data were stratified by oncological and non-oncological diseases. Results The analysis showed that of the 870 patients referred to the Padua Pediatric Hospice between 2008 and 2022, 76% were affected by non-oncological conditions. 82% of patients referred were taken in charge and most of the remaining patients have been inappropriately referred. The analysis showed a growing number of total referrals, which increased by 195% from 2008 to 2022. An increase in proper referrals and referrals of non-oncological patients was observed alongside a decrease in oncological patient referrals and a trend toward a decrease in discharge rates. A decreased mortality was observed in patients with non-oncological conditions, with only 6% of deceased patients in 2022. Moreover, a longer survival with a median follow-up length of 43 months was observed among patients with non-oncological conditions who were followed up at the Padua Pediatric Hospice for more than 12 months. Conversely, the short survival rate observed for oncological patients suggests that those patients should have been referred to PPC earlier to benefit from palliative care for longer periods. Conclusions Considering these data, it is expected that the number of patients needing PPC services will steadily increase in the next years. Hence, there is a need to invest resources to provide the best care delivery model encompassing specific pathways for the transition into adulthood, the establishment of networks within all the Italian regions, and an efficient referral to the more suitable setting of care.

Abstract The study of species groups in which the presence of interspecific hybridization or introgression phenomena is known or suspected involves analysing shared bi-parentally inherited molecular markers. Current methods are based on different categories of markers among which the classical microsatellites or the more recent genome wide approaches for the analyses of thousands of SNPs or hundreds of microhaplotypes through high throughput sequencing. Our approach utilizes intron-targeted amplicon sequencing to characterise multi-locus intron polymorphisms (MIPs) and assess genetic diversity. These highly variable intron regions, combined with inter-specific transferable loci, serve as powerful multiple-SNP markers potentially suitable for various applications, from species and hybrid identification to population comparisons, without prior species knowledge. We developed the first panel of MIPs highly transferable across fish genomes, effectively distinguishing between species, even those closely related, and populations with different structures. MIPs offer versatile, hypervariable nuclear markers and promise to be especially useful when multiple nuclear loci must be genotyped across different species, such as for the monitoring of interspecific hybridization. Moreover, the relatively long sequences obtained ease the development of single-locus PCR-based diagnostic markers. This method, here demonstrated in teleost fishes, can be readily applied to other taxa, unlocking a new source of genetic variation.

Introduction of non-native species is an important cause of biodiversity decline in rivers. Separated by mountains and salt water, the freshwater fish fauna in Italy has experienced a natural isolation from fish in continental Europe. As a consequence, several Italian fish species have diverged from their European sister species, likely with unique adaptations to the local environment. Relatedly, the region is also susceptible to the invasion of non-native fish, and today almost half the fish species present are of non-native origin. Several of these non-native species have Italian congeners susceptible to competition and hybridizations, and, in the long run, displacements and extinctions. One such example is the Italian gudgeon (Gobio benacensis) and its European congener European gudgeon (Gobio gobio). During the last few decades the European gudgeon was introduced in Italian waters and has since spread rapidly, causing progressive declines in the Italian species. As for several other similar species pairs, little is known about potential differences in ecology and behavior. Here we study differences between Italian and European gudgeons in a controlled laboratory environment, using a combined open field and provoked escape response test, as well as tracking their sympatric survival over time in the hatchery. The smaller Italian gudgeon displayed a lower maximum swimming speed compared to the larger European gudgeon. The Italian gudgeon also experienced substantially higher sympatric mortality, resulting in only European gudgeon surviving to the end of the experiment. Contrary to expectations, no difference was seen in boldness and the Italian gudgeon displayed a higher movement activity, moving a larger distance in an open field test, compared to its European sister species. The reported differences could play a role in the replacement process, and may also have impacts on the surrounding ecosystem, and the prey and predators that have coevolved with the Italian species.

Abstract International building certification systems, such as the WELL and Leadership in Energy and Environmental Design (LEED) standards, play a pivotal role in the design of healthy and sustainable buildings. While LEED adopts a holistic approach to designing healthy and sustainable buildings, the WELL standard has a strong emphasis on human health, comfort, and well-being. Although prior research has revealed inconsistent results for occupant satisfaction in office buildings with WELL certification compared to buildings without WELL certification, or are certified using another certification system (e.g., LEED), most of these comparisons tend to lack methodological rigor. This study used a statistical procedure to match and compare 1634 occupant surveys from LEED-certified buildings to 1634 surveys from WELL-certified buildings. Six important architectural and experiential parameters were matched, masking their influence on the outcome. Overall building and workspace satisfaction was high in both WELL-certified buildings (94% and 87%) and LEED-certified (73% and 71%). We found that there is a 39% higher probability of finding occupants who are more satisfied in WELL-certified buildings compared to LEED-certified buildings, indicating occupant satisfaction is higher in buildings with WELL certification. Although we were unable to pinpoint the reason for higher occupant satisfaction in WELL-certified buildings, the results consistently showed that perceived indoor environmental quality was enhanced across all parameters except for the amount of space.

ABSTRACT: This study aimed to set up a life cycle assessment (LCA) approach at the level of individual animals to assess the effects of a 3-breed crossbreeding program on the environmental impact of cows. The study involved 564 cows, 279 purebred Holstein Friesian (HO) and 285 crossbred cows (CR), that originated from a 3-breed crossbreeding program based on the rotational use of Viking Red, Montebèliarde, and HO sires and kept in 2 dairy herds of northern Italy (224 and 340 cows/herd, respectively). The reference unit of the LCA model was the lifetime of cows, from the birth to culling or death. Data were collected at different levels: individual animal-based data referred to the whole life (birth, calving, dry, cull or death dates, and milk production); individual test-date collection of body measures and BCS, used to predict BW and to estimate energy requirements; common farm-based data concerning herd management (diets composition, and materials used). Data were used to compute DMI, milk and milk components production, gross income (GI), and income over feed costs (IOFC) pertaining to the lifespan of cows. An individual LCA-derived approach was set up to compute global warming potential (GWP), acidification and eutrophication potential (AP and EP, respectively), and land occupation (LO), which have been associated with different functional units (cow in her whole life or per day of life; kilograms of milk fat plus protein, and GI and IOFC [in euros] produced in the herd life). Data were analyzed using a generalized linear model including the fixed effects of genetic group (CR vs. HO), farm, and their interaction (genetic group × farm). Compared with HO, CR cows completed more lactations (+12%), had earlier first calving (−2 wk), yielded more fat plus protein in milk both in the lifespan (+8%) and per day of life (+4%). Concerning the environmental impact, when compared with HO herd mates, CR cows had nominal greater emissions per cow in the whole life, similar emissions per day of life and ∼3% lower GWP, AP, and EP per kilogram of fat plus protein yielded in lifespan. Income over feed costs per unit of emission tended to be ∼4% greater in CR compared with HO cows. Also, the use of land tended to be lower in CR compared with HO in most indicators considered. In conclusion, LCA could be adapted to represent individual animals. Moreover, managing dairy cows according to a 3-breed rotational crossbreeding scheme may be regarded as a strategy that can contribute to mitigate the emissions and to improve the environmental impact of dairy operations.

Abstract Background Various blood metabolites are known to be useful indicators of health status in dairy cattle, but their routine assessment is time-consuming, expensive, and stressful for the cows at the herd level. Thus, we evaluated the effectiveness of combining in-line near infrared (NIR) milk spectra with on-farm (days in milk [DIM] and parity) and genetic markers for predicting blood metabolites in Holstein cattle. Data were obtained from 388 Holstein cows from a farm with an AfiLab system. NIR spectra, on-farm information, and single nucleotide polymorphisms (SNP) markers were blended to develop calibration equations for blood metabolites using the elastic net (ENet) approach, considering 3 models: (1) Model 1 (M1) including only NIR information, (2) Model 2 (M2) with both NIR and on-farm information, and (3) Model 3 (M3) combining NIR, on-farm and genomic information. Dimension reduction was considered for M3 by preselecting SNP markers from genome-wide association study (GWAS) results. Results Results indicate that M2 improved the predictive ability by an average of 19% for energy-related metabolites (glucose, cholesterol, NEFA, BHB, urea, and creatinine), 20% for liver function/hepatic damage, 7% for inflammation/innate immunity, 24% for oxidative stress metabolites, and 23% for minerals compared to M1. Meanwhile, M3 further enhanced the predictive ability by 34% for energy-related metabolites, 32% for liver function/hepatic damage, 22% for inflammation/innate immunity, 42.1% for oxidative stress metabolites, and 41% for minerals, compared to M1. We found improved predictive ability of M3 using selected SNP markers from GWAS results using a threshold of > 2.0 by 5% for energy-related metabolites, 9% for liver function/hepatic damage, 8% for inflammation/innate immunity, 22% for oxidative stress metabolites, and 9% for minerals. Slight reductions were observed for phosphorus (2%), ferric-reducing antioxidant power (1%), and glucose (3%). Furthermore, it was found that prediction accuracies are influenced by using more restrictive thresholds (−log10(P-value) > 2.5 and 3.0), with a lower increase in the predictive ability. Conclusion Our results highlighted the potential of combining several sources of information, such as genetic markers, on-farm information, and in-line NIR infrared data improves the predictive ability of blood metabolites in dairy cattle, representing an effective strategy for large-scale in-line health monitoring in commercial herds.

The effects of ceiling fans on the transmission of infectious aerosols remain poorly understood, leading to conflicting recommendations. We conducted repeated experiments in a well-controlled chamber with a typical mixing ventilation system at three different ventilation rates with and without ceiling fans. We evaluated airborne infection risks for short- and long-range transmission routes based on size-resolved tracer particles measured at various locations. We found that the mixing ventilation without fans only effectively diluted the airborne particle concentration for the long-range route but not for the short-range. By using ceiling fans to enhance air mixing, tracer particles were distributed more homogeneously throughout the room, leading to up to 77 % reduction in short-range particle exposure while a slight increase of less than 14 % in long-range exposure. Based on the dilution-based Wells-Riley model, the changes in particle concentration translated to a maximum 47 % reduction in short-range infection risk and a marginal 4 % increase for long-range transmission. Based on the dilution factors obtained from the experiments, we developed a decision-making tool that uses the ventilation rate, the number of individuals at short- and long-range, and the disease's transmissibility to decide whether the use of ceiling fans is beneficial. Deploying ceiling fans always reduces the concentration of particles in the short range and, assuming a relationship between particles and pathogens, this directly translates to a diminished short-range risk. Based on the modeling of the overall risk, the benefits of fans are highest when the room is ventilated according to code, when masking measures are in place, and when the pathogen is not highly contagious.

Research on triethylene glycol (TEG) use to disinfect airborne microorganisms have been conducted in non-realistic chamber settings. This study assesses how air temperature, humidity, HVAC settings, and filtration impact TEG's effectiveness in deactivating a common SARS-CoV-2 substitute, MS2 bacteriophage, in a simulated non-occupied office-sized chamber. The chamber was served by a dedicated HVAC system operating at 22.0, 23.5 and 25.0 °C, at 40, 55 and 70 % relative humidity, at 0, 3 and 6 air change per hour (ACH) recirculation, at 0.8, 2.5 and 5.0 ACH outdoor ventilation and at no, MERV8 and MERV14 filtration status. Airborne MS2 log10 reductions in the presence of TEG increased linearly over time and we noted a higher MS2 inactivation rate with higher TEG concentration. The estimated TEG concentration needed for a one-log inactivation of the MS2 within an hour was 0.44 mg/m3. The efficacy of TEG declined with the increase in temperature from 22.0 to 25.0 °C, peaked at 55 % RH, increased with higher recirculation rates but decreased with increasing ventilation rates and higher efficiency filters. The results of our study suggest that the optimum environmental and building conditions for TEG performance is at 22.0 or 23.5 °C air temperature, 55 % relative humidity, 0.8 ACH ventilation rate and 6 ACH recirculation rate. By conducting experiments in simulated office conditions, this study closes significant knowledge gaps in TEG performance application.

Introdução: Estima-se que 1/4 da população mundial esteja infectada com Mycobacterium tuberculosis, com maior risco de doença concentrado nos primeiros anos após a infecção primária, porém o período de latência pode durar anos. Tanto a infecção pelo HIV como o envelhecimento são fatores de risco para tuberculose ativa(Tb). No entanto, existe uma lacuna na compreensão do risco de Tb na população de pessoas idosas que vivem com HIV(PIVHA). Objetivo: Avaliar a prevalência de infecção latente por tuberculose(ILTB) em PIVHA e fatores associados. Método: Estudo incluiu PIVHA (≥ 50 anos) em terapia antirretroviral em quatro centros de 3 cidades brasileiras. Exames laboratoriais, incluindo IGRA (Interferon-gama release assay) para ILTB foram realizados. Aqueles com história documentada de tuberculose ativa/tratada ou com resultado de IGRA ausente/inconclusivo foram excluídos. ILTB foi definida por IGRA positivo no baseline ou IGRA ou PPD(prova tuberculínica) previamente positivo. As razões de prevalência de ILTB foram avaliadas por modelos de regressão de Poisson modificados não ajustados e multivariados, considerando covariáveis como idade, sexo ao nascer, raça, anos de diagnóstico de HIV, cidade de residência, escolaridade, renda, insegurança alimentar, uso atual de álcool/substâncias/tabaco e contagem de células TCD4 no baseline. Resultados: Excluidos 158 participantes por histórico de Tb ativa e 72 por resultados testes de ILTB ausentes/inconclusivos. 473 PIVHA foram incluídos na análise. Idade média de 62 anos; 293 (62%) sexo masculino ao nascer; 129 (29%) pardos e 101 (21%) negros. A mediana de anos de diagnóstico de HIV foi de 23; mediana do número de residentes no domicílio foi 2; 110 (23%) relataram renda familiar mensal < 1 salário mínimo; 95 (20%) tinham < 8 anos de escolaridade; e a mediana da contagem de células TCD4 foi de 752 células/μL. A prevalência de ILTB foi de 169/473 (36%), variando entre as cidades (44% Rio de Janeiro, 38% São Paulo e 23% Salvador). Na análise multivariada, contagem de células TCD4 (< 500 vs. ≥ 900 aPR:0,64 [IC95% 0,45-0,93]) e renda (< 1 vs 1-3 salários mínimos aPR:1,53 [IC95% 1,09-3.14]) foram associados ao risco de ILTB. Conclusão: Encontramos elevada prevalência de ILTB em PIVHA, particularmente associada à contagem de células TCD4 e baixa renda. Estes dados evidenciam a urgência de iniciativas para aumentar o acesso a testes de diagnóstico de ILTB e ao seu tratamento para reduzir o risco de doença e morte por tuberculose nesta população.

Introdução: Com a limitação de esquemas terapêuticos para individualização do tratamento do HIV no Brasil pelo Sistema Único de Saúde, a simplificação é uma das estratégias de individualização para pessoas que vivem com HIV (PVHA) com comorbidades-toxicidades. Objetivo: Avaliar fatores associados a indicação de simplificação para terapia dupla baseada em Dolutegravir + Lamivudina. Método: Estudo de coorte retrospectivo avaliou PVHA no CRT DST/AIDS, São Paulo, utilizando Dolutegravir + Lamivudina por ≥48 semanas. Os dados foram coletados de prontuários, sistema de controle de medicamentos e de controle laboratorial e inseridos na plataforma REDCap. A análise foi realizada utilizando modelo de regressão de Poisson modificado não ajustado e multivariado. Resultados: No serviço, de 7.000 PVHA, 919 eram elegíveis. A mediana de uso de TD (terapia dupla) foi de 26,2 meses. Mediana de idade de 50,8 anos, com 82,3% designados do sexo masculino ao nascer; 69,3% raça branca. Tempo médio desde o diagnóstico de HIV foi 11,8 anos; tempo médio de exposição à TARV 9,1 anos; mediana de regimes de TARV anteriores foi de 3. Principais motivos para uso de TD: conveniência posológica (29,9%), comorbidades-toxicidades renais (21,8%) e ósseas (21,7%). Em relação à conveniência posológica, indivíduos com ≤ 5 anos de uso de TARV tiveram probabilidade 1,7 vezes maior de iniciar TD do que aqueles com ≥ 15 anos (RP = 1,69; IC95%: 1,26 – 2,27, p < 0,001). Para comorbidade-toxicidade renal, raça e idade foram fatores independentes para TD (p = 0,006 e p = 0,004). A raça negra teve chance 1,7 vezes maior de TD do que a branca (RP = 1,74; IC95%: 1,18 – 2,57), e aqueles com idade entre 50 e 69 anos tiveram chance 2,6 maior em comparação com 18 a 29 anos (RP = 2,58; IC95%: 1,48 – 4,45). Sexo e idade foram fatores independentes para início de TD em relação a comorbidade-toxicidade óssea (p = 0,001 e p < 0,001). Indivíduos do sexo feminino ao nascer tiveram chance 1,6 vezes maior de uso de TD do que do sexo masculino (RP = 1,57; IC95%: 1,21 – 2,04), e aqueles com idade entre 50 e 69 anos tiveram probabilidade 3,9 maior em comparação aos de 18 a 29 anos (RP = 3,92; IC95%: 1,87 – 8,18). Conclusão: O estudo destaca as comorbidades-toxicidades como os principais motivos do uso de TD em idosos que vivem com HIV. Estes achados estão alinhados com as diretrizes nacionais e podem reforçar o desenvolvimento de políticas de saúde pública, enfatizando a importância de abordagens terapêuticas personalizadas para PVHA.

Introdução: Terapia dupla (TD) com dolutegravir + lamivudina é aprovado no Brasil para uso em pessoas que vivem com HIV (PVHA) em uso de terapia antirretroviral (TARV), virologicamente suprimidos, com contra indicação ou risco de desenvolver toxicidade/evento adverso relacionado aos ITRNT (Inibidores da transcriptase reversa análogos de núcleosídeos/nucleotídeos). A eficácia desta estratégia foi demonstrada em estudos clínicos e em coortes de vida real, principalmente na Europa e USA, com poucos dados da América Latina. Objetivo: Avaliar a eficácia na manutenção da supressão viral (SV) numa coorte de PVHA, sem teste prévio de resistência. Método: Coorte retrospectiva de PVHA acompanhadas no CRT-DST/AIDS-SP, em uso de TD baseado em Dolutegravir + Lamivudina por ≥ 48 semanas. Dados captutados de prontuário médico, SICLOM, SISCEL e inseridos na plataforma REDCAP. A análise foi realizada utilizando plataforma STATA 17.0 na população do estudo por intenção de tratar (ITT) e por protocolo (PP), excluindo óbitos e perdas de seguimento. Resultados: De 7.000 PVHA, 919 eram elegíveis, tempo médio de TD de 26,2 meses. 82,9% (757)-homens cisgêneros, 69,3% (637)-raça branca, idade 50,8 anos. Tempo médio de infecção pelo HIV de 11,8 anos, de exposição aos ARV 9,1 anos e regimes ARVs prévios 2. Os principais motivos para TD foram: simplificação 29,9%, comorbidade/toxicidade renal 21,8% e óssea 21,7%. Desfechos da TD: 872 PVHA mantidos em TD, 15 mortes, 13 perdas de seguimento e 19 mudanças de terapia, sendo 7 eventos adversos, 3 erros de indicação, 2 decisões médicas e 7 desfechos virológicos (6 falhas virológicas (FV) em pacientes multi-experimentados e 1 blip). As taxas de manutenção da TD no período do estudo nas populações ITT e PP foram de 94,9% e 97,8%, respectivamente. A taxa de FV na população PP foi de 0,7%. Na análise de Kaplan-Meier e Cox, variáveis independentemente associadas à manutenção da TD foram sexo ao nascer (p = 0,008), idade (p < 0,001), raça (p = 0,006). Maior risco de descontinuação da TD: genêro masculino (p = 0,010), raça negra/parda (p = 0,002/ p = 0,006) e motivo da indicação de TD ser simplificação (p = 0,024). Idosos foram relacionados a maiores taxas de manutenção de TD. O tempo de exposição aos ARVs não foi relacionado à manutenção de TD nas duas análises. Conclusão: O estudo fornece evidências reais que apoiam o uso da TD no Brasil, com alta taxa de manutenção da SV(97,8%), baixa taxa de FV(0,7%), tornando-a uma opção segura mesmo para PVHA sob tratamento antirretroviral há muito tempo.

BackgroundERAS is an evidence-based multimodal perioperative protocol focused on stress reduction and promoting a return to function. The aim of this work is to perform a cost-consequence analysis for the implementation of ERAS in major lung resection by means of minimally invasive surgery (VATS) from the public health service perspective, evaluating resource consumption and clinical outcomes with respect to a control group of past patients, which did not adopt an ERAS protocol.MethodsOutcome differences (re-intervention rates, major and minor intraoperative and postoperative complications, readmissions, and mortality) as well as the costs of preoperative, operative, and postoperative care were estimated. The sample consisted of 64 consecutive patients enrolled in the ERAS programme between April 2021 and August 2022, compared to a control group (historical cohort) comprising 31 patients treated from April 2020 to December 2020, prior to the implementation of the ERAS programme. The study sample comprises patients who fulfil the established ERAS protocol inclusion criteria, including general criteria (acceptance of the protocol, proximity of residence, absence of contraindications to physiotherapy and early mobilisation), surgical criteria (anatomical lung resection up to lobectomy, absence of extensive resection, good possibility of conducting the operation in VATS) and anaesthesiologic criteria (ASA ≤2). Costs were quantified using the national health system perspective.ResultsThe average length-of-stay was at least one day shorter in the ERAS group [

Devastation from hurricanes can cause multiple problems for turfgrass managers, sod producers, and homeowners. Damage may include visible factors such as prolonged periods of standing water, uprooted trees, shading and obstruction from demolished structures, deposition of silt or mud, and damage to irrigation systems. Less visible problems may include damage to root systems resulting from compacted soil from heavy equipment used during cleanup and recovery, reduced drainage, and injury resulting from effects of saltwater intrusion. Suggested guidelines for correcting these problems are included in the text.

Devastation from hurricanes can cause multiple problems for turfgrass managers, sod producers, and homeowners. Damage may include visible factors such as prolonged periods of standing water, uprooted trees, shading and obstruction from demolished structures, deposition of silt or mud, and damage to irrigation systems. Less visible problems may include damage to root systems resulting from compacted soil from heavy equipment used during cleanup and recovery, reduced drainage, and injury resulting from effects of saltwater intrusion. Suggested guidelines for correcting these problems are included in the text.

ABSTRACT Objective: This study evaluated the force system generated by the Memory Titanol® spring (MTS) with different preactivation bends using an orthodontic force tester (OFT). Methods: Three preactivations were tested using a 0.017 × 0.022-in stainless steel (SS) wire and a 0.018 × 0.025-in NiTi segment, with an activation of 30º in the posterior segment (β), with 0º (Group 1 [G1]), 45º (Group 2 [G2]), and 60º (Group 3 [G3]) in the anterior segment (α). Results: The molars showed extrusion values of −1.33 N for G1 and −0.78 N for G2, and an intrusion value of 0.33 N for G3. The force in the premolars was intrusive with a variation of 1.34 N for G1 and 0.77 N for G2; and extrusive with a variation of −0.31 N for G3. Regarding the upright moment (Ty) of the molar, a distal moment was observed with values of 53.45 N.mm for G1 and 19.87 N.mm for G2, while G3 presented a mesial moment of −6.23 N.mm. G1, G2, and G3 all exhibited distal premolar moments (Ty) of 3.58, 2.45, and 0.68 N.mm, respectively. Conclusions: The tested preactivations exerted an extrusive force in G1 and G2 and an intrusive force in G3 during molar vertical movement. The premolar region in G1 and G2 showed intrusive force and distal moment.

O presente artigo escrito em formato de memorial, texto no qual os autores evocam fatos a que tenham assistido ou vivenciado, se propõe a descrever e analisar o processo de criação do espetáculo Santo Qorpo ou O Louco da Província, realizado em 2014, por um grupo de estudantes do curso de Teatro do Departamento de Arte Dramática (DAD), da Universidade Federal do Rio Grande do Sul (UFRGS) sob a orientação da Profa. Dra.(xx). O objetivo central desta pesquisa prática e teórica, ainda em curso, segue sendo evocar a obra e história do dramaturgo Qorpo Santo na cidade de Porto Alegre.

A Set of new MPGD-based Photon Detectors is being built for the upgrade of COMPASS RICH-1. The detectors cover a total active area of 1.4 m2 and are based on a hybrid architecture consisting of two THGEM layers and a Micromegas. A CsI film on one THGEM acts as a reflective photocathode. The characteristics of the detector, the production of the components and their validation tests are described in detail.

Abstract Sleep is a pillar of human health and wellbeing. In high- and middle-income countries, there is a great reliance on heating, ventilation, and air conditioning systems (HVAC) to control the interior thermal environment in the bedroom. However, these systems are expensive to buy, maintain, and operate while being energy and environmentally intensive—problems that may increase due to climate change. Easily-accessible passive and low-energy strategies, such as fans and electrical heated blankets, address these challenges but their comparative effectiveness for providing comfort in sleep environments has not been studied. We used a thermal manikin to experimentally show that many passive and low-energy strategies are highly effective in supplementing or replacing HVAC systems during sleep. Using passive strategies in combination with low-energy strategies that elevate air movement like ceiling or pedestal fans enhances the cooling effect by three times compared to using fans alone. We extrapolated our experimental findings to estimate heating and cooling effects in two historical case studies: the 2015 Pakistan heat wave and the 2021 Texas power crisis. Passive and low-energy strategies reduced sleep-time heat or cold exposure by 69–91%. The low-energy strategies we tested require one to two orders of magnitude less energy than HVAC systems, and the passive strategies require no energy input. These strategies can also help reduce peak load surges and total energy demand in extreme temperature events. This reduces the need for utility load shedding, which can put individuals at risk of hazardous heat or cold exposure. Our results may serve as a starting point for evidence-based public health guidelines on how individuals can sleep better during heat waves and cold snaps without relying on HVAC.

Abstract The amazing complexity of gene regulatory circuits, and biological systems in general, makes mathematical modeling an essential tool to frame and develop our understanding of their properties. Here, we present some fundamental considerations to develop and analyze a model of a gene regulatory circuit of interest, either representing a natural, synthetic, or theoretical system. A mathematical model allows us to effectively evaluate the logical implications of our hypotheses. Using our models to systematically perform in silico experiments, we can then propose specific follow-up assessments of the biological system as well as to reformulate the original assumptions, enriching both our knowledge and our understanding of the system. We want to invite the community working on different aspects of gene regulatory circuits to explore the power and benefits of mathematical modeling in their system.

Due to their high tensile strength and toughness, natural fibers have been proposed as reinforcing material in mortars to reduce cracking. Coir fiber has excellent potential for reuse in cementitious matrices among the various fiber types. However, this material is hydrophilic and has a high extractive content, including wax, oils, and other components in the structure. Different chemical treatments are used to remove contaminants from the fiber surface of the coir to minimize the presence of these elements. This study evaluated chemically treated coir fibers in mortars based on cement and lime. The effects of two levels of coir addition (1% and 2% relative to cement mass) treated with oxalic acid at a concentration of 5% w/w for 2 h and sodium bicarbonate at 10% w/w for 120 h were evaluated. The effect of these variables on compressive and tensile strength, capillary water absorption, accelerated carbonation, and microstructure analysis using X-ray microtomography and scanning electron microscopy were investigated. The results showed that with the addition of 2% coir treated with oxalic acid, mortars increased compressive strength by 8% and 22% in the tensile strength. Moreover, it was found that the treatment minimizes the carbonation rate in mortars with coir fibers, whose pore size distribution is also affected by the presence of the fibers and the treatment type. It can be concluded that the fiber's chemical treatment improves its properties for use in mortars, considering a synergistic analysis, and can be used as an alternative for composite material production.

Abstract Background Among people living with HIV, elite controllers (ECs) maintain an undetectable viral load, even without receiving anti-HIV therapy. In non-EC patients, this therapy leads to marked improvement, including in immune parameters, but unlike ECs, non-EC patients still require ongoing treatment and experience co-morbidities. In-depth, comprehensive immune analyses comparing EC and treated non-EC patients may reveal subtle, consistent differences. This comparison could clarify whether elevated circulating interferon-alpha (IFNα) promotes widespread immune cell alterations and persists post-therapy, furthering understanding of why non-EC patients continue to need treatment. Methods Levels of IFNα in HIV-infected EC and treated non-EC patients were compared, along with blood immune cell subset distribution and phenotype, and functional capacities in some cases. In addition, we assessed mechanisms potentially associated with IFNα overload. Results Treatment of non-EC patients results in restoration of IFNα control, followed by marked improvement in distribution numbers, phenotypic profiles of blood immune cells, and functional capacity. These changes still do not lead to EC status, however, and IFNα can induce these changes in normal immune cell counterparts in vitro. Hypothesizing that persistent alterations could arise from inalterable effects of IFNα at infection onset, we verified an IFNα-related mechanism. The protein induces the HIV coreceptor CCR5, boosting HIV infection and reducing the effects of anti-HIV therapies. EC patients may avoid elevated IFNα following on infection with a lower inoculum of HIV or because of some unidentified genetic factor. Conclusions Early control of IFNα is essential for better prognosis of HIV-infected patients.

Abstract Background A complete understanding of the different steps of HIV replication and an effective drug combination have led to modern antiretroviral regimens that block HIV replication for decades, but these therapies are not curative and must be taken for life. “Elite controllers” (ECs) is a term for the 0.5% of HIV-infected persons requiring no antiretroviral therapy, whose status may point the way toward a functional HIV cure. Defining the mechanisms of this control may be key to understanding how to replicate this functional cure in others. Methods In ECs and untreated non-EC patients, we compared IFNα serum concentration, distribution of immune cell subsets, and frequency of cell markers associated with immune dysfunction. We also investigated the effect of an elevated dose of IFNα on distinct subsets within dendritic cells, natural killer cells, and CD4+ and CD8 + T cells. Results Serum IFNα was undetectable in ECs, but all immune cell subsets from untreated non-EC patients were structurally and functionally impaired. We also show that the altered phenotype and function of these cell subsets in non-EC patients can be recapitulated when cells are stimulated in vitro with high-dose IFNα. Conclusions Elevated IFNα is a key mediator of HIV pathogenesis.

ABSTRACT: The considerable increase in the production capacity of individual cows owing to both selective breeding and innovations in the dairy sector has posed challenges to management practices in terms of maintaining the nutritional and metabolic health status of dairy cows. In this observational study, we investigated the associations between milk yield, composition, and technological traits and a set of 21 blood biomarkers related to energy metabolism, liver function or hepatic damage, oxidative stress, and inflammation or innate immunity in a population of 1,369 high-yielding Holstein-Friesian dairy cows. The milk traits investigated in this study included 4 production traits (milk yield, fat yield, protein yield, daily milk energy output), 5 traits related to milk composition (fat, protein, casein, and lactose percentages and urea), 11 milk technological traits (5 milk coagulation properties and 6 curd-firming traits). All milk traits (i.e., production, composition, and technological traits) were analyzed according to a linear mixed model that included the days in milk, the parity order, and the blood metabolites (tested one at a time) as fixed effects and the herd and date of sampling as random effects. Our findings revealed that milk yield and daily milk energy output were positively and linearly associated with total cholesterol, nonesterified fatty acids, urea, aspartate aminotransferase, γ-glutamyl transferase, total bilirubin, albumin, and ferric-reducing antioxidant power, whereas they were negatively associated with glucose, creatinine, alkaline phosphatase, total reactive oxygen metabolites, and proinflammatory proteins (ceruloplasmin, haptoglobin, and myeloperoxidase). Regarding composition traits, the protein percentage was negatively associated with nonesterified fatty acids and β-hydroxybutyrate (BHB), while the fat percentage was positively associated with BHB, and negatively associated with paraoxonase. Moreover, we found that the lactose percentage increased with increasing cholesterol and albumin and decreased with increasing ceruloplasmin, haptoglobin, and myeloperoxidase. Milk urea increased with an increase in cholesterol, blood urea, nonesterified fatty acids, and BHB, and decreased with an increase in proinflammatory proteins. Finally, no association was found between the blood metabolites and milk coagulation properties and curd-firming traits. In conclusion, this study showed that variations in blood metabolites had strong associations with milk productivity traits, the lactose percentage, and milk urea, but no relationships with technological traits of milk. Specifically, increasing levels of proinflammatory and oxidative stress metabolites, such as ceruloplasmin, haptoglobin, myeloperoxidase, and total reactive oxygen metabolites, were shown to be associated with reductions in milk yield, daily milk energy output, lactose percentage, and milk urea. These results highlight the close connection between the metabolic and innate immunity status and production performance. This connection is not limited to specific clinical diseases or to the transition phase but manifests throughout the entire lactation. These outcomes emphasize the importance of identifying cows with subacute inflammatory and oxidative stress as a means of reducing metabolic impairments and avoiding milk fluctuations.

ABSTRACT: Mastitis is one of the most significant diseases in dairy cows and causes several economic losses. Somatic cell count (SCC) is often used as an indirect diagnostic tool for mastitis, especially for subclinical mastitis (SCM) where no symptoms or signs can be detected. Streptococcus agalactiae is one of the main causes of contagious mastitis, and Prototheca spp. is an alga-inducing environmental mastitis that is not always correlated with increased milk SCC. The aim of this study was to evaluate the changes in the metabolomic profile of blood in relation to subclinical intramammary infection (IMI) in dairy cows. In addition, differences resulting from the etiologic agent causing mastitis were also considered. Forty Holstein-Friesian dairy cows in mid and late lactation were enrolled in this cross-sectional design study. Based on the bacteriological examination of milk, the animals were divided into 3 groups: group CTR (control group; n = 16), group A (affected by SCM with IMI caused by Strep. agalactiae; n = 17), and group P (affected by SCM with IMI caused by Prototheca spp.; n = 7). Blood samples from the jugular vein were collected in tubes containing clot activator; the serum aliquot was stored until metabolomic analysis by 1H-nuclear magnetic resonance spectroscopy. Statistical analysis was conducted by fitting a linear model with the group as the fixed effect and SCC as the covariate. Forty-two metabolites were identified, and among them 10 were significantly different among groups. Groups A and P showed greater levels of His and lactose and lower levels of acetate, Asn, and dimethylamine compared with group CTR. Group A showed high levels of Val, and group P showed high levels of Cit and methylguanidine, as well as lower levels of 3-hydroxybutyrate, acetone, allantoin, carnitine, citrate, and ethanol. These metabolites were related to ruminal fermentations, energy metabolism, urea synthesis and metabolism, immune and inflammatory response, and mammary gland permeability. These results suggest systemic involvement with subclinical IMI and that the metabolic profile of animals with SCM undergoes changes related to the etiological agent of mastitis.

ABSTRACT: In this study we wanted to investigate the associations between naturally occurring subclinical intramammary infection (IMI) caused by different etiological agents (i.e., Staphylococcus aureus, Streptococcus agalactiae, Streptococcus uberis, and Prototheca spp.), in combination with somatic cell count (SCC), on the detailed milk protein profile measured at the individual mammary gland quarter. An initial bacteriological screening (time 0; T0) conducted on individual composite milk from 450 Holstein cows reared in 3 herds, was performed to identify cows with subclinical IMI. We identified 78 infected animals which were followed up at the quarter level at 2 different sampling times: T1 and T2, 2 and 6 wk after T0, respectively. A total of 529 quarter samples belonging to the previously selected animals were collected at the 2 sampling points and analyzed with a reversed phase HPLC (RP-HPLC) validated method. Specifically, we identified and quantified 4 caseins (CN), namely αS1-CN, αS2-CN, κ-CN, and β-CN, and 3 whey protein fractions, namely β-lactoglobulin, α-lactalbumin, and lactoferrin (LF), which were later expressed both quantitatively (g/L) and qualitatively (as a percentage of the total milk nitrogen content, % N). Data were analyzed with a hierarchical linear mixed model with the following fixed effects: days in milk (DIM), parity, herd, SCC, bacteriological status (BACT), and the SCC × BACT interaction. The random effect of individual cow, nested within herd, DIM and parity was used as the error term for the latter effects. Both IMI (i.e., BACT) and SCC significantly reduced the proportion of β-CN and αS1-CN, ascribed to the increased activity of both milk endogenous and microbial proteases. Less evident alterations were found for whey proteins, except for LF, which being a glycoprotein with direct and undirect antimicrobial activity, increased both with IMI and SCC, suggesting its involvement in the modulation of both the innate and adaptive immune response. Finally, increasing SCC in the positive samples was associated with a more marked reduction of total caseins at T1, and αS1-CN at T2, suggesting a synergic effect of infection and inflammation, more evident at high SCC. In conclusion, our work helps clarify the behavior of protein fractions at quarter level in animals having subclinical IMI. The inflammation status driven by the increase in SCC, rather the infection, was associated with the most significant changes, suggesting that the activity of endogenous proteolytic enzymes related to the onset of inflammation might have a pivotal role in directing the alteration of the milk protein profile.

La grama centípeda, centipedegrass (en inglés) o pasto ciempiés (Eremochloa ophiuroides [Munro] Hack.) se introdujo en los Estados Unidos desde el sureste asiático en 1916. Este pasto está bien adaptado al clima y los suelos del centro y norte de Florida y es el césped residencial más común en el noroeste de Florida. Esta publicación está destinada a entusiastas de césped y paisajismo de Florida, y profesionales en mantenimiento de pasto ciempiés en paisaje residencial y comercial.

Abstract The objective of this work was to evaluate physical and chemical fruit parameters of new strawberry genotypes underfield conditions. The study was carried out during the 2018 and 2019 seasons. Twelve genotypes from the breeding program of Embrapa and two commercial cultivars (Camino Real and Merced) were evaluated. The assessed parameters were: average fruit number, average fruit weight, soluble solids, titrable acidity, and the soluble solids/titrable acidity ratio. Among the studied strawberry genotypes, the 8-1, 35-22 ('BRS DC22'), 31-13, 7-5, and 32-2 selections produce more fruits per plant. Among the new genotypes, 'BRS DC25' (Fênix) and 31-13 present the best average fruit weight in both evaluated seasons. The highest sugar content is observed in genotypes 35-22 ('BRS DC22') and 65-2, whereas 8-1 and 'BRS DC25' (Fênix) show the highest acidity. The 7-5, 35-22 ('BRS DC22'), 34-2, and 35-6 genotypes present the best soluble solid/acidity ratio.

ABSTRACT Objective: This descriptive observational study aimed to determine clinically relevant and applicable data of enamel thickness (ETH), considering the mesio-distal differences of anterior and posterior permanent teeth and their relationships. Material and Methods: The sample consisted of right-sided standardized radiographs of 34 individuals (21 females and 13 males), aged between 13 and 24 (average 16) years, with all permanent teeth intact and without crowding. Four periapical and four interproximal radiographs were obtained and digitized. ETH measurements (mesial to distal contact points at the dentin-enamel junction) were performed after correction for radiographic image magnification. The Students’ t-test was applied to the differences between paired means, with the Pearson correlation to evaluate the correlation between them. Results: The mesial and distal ETH increased from the anterior to the posterior teeth. Incisor ETH ranged between 0.60 and 0.84 mm. Canines, premolars, and molars were more than 1.0 mm thick, and molar enamel reached values between 1.26 and 1.44 mm. Conclusion: Distal ETH was significantly greater than the mesial ETH, and progressively thicker from the anterior to posterior teeth. Interproximal reduction (IPR) of the lower central and upper lateral incisors should be avoided, reduced, or performed on their distal surfaces. There is a positive and significant correlation between ETH and the mesial and distal surfaces of the teeth. Periapical radiographs and evaluation of the remaining ETH are necessary in cases of retreatment. The location and number of tooth size discrepancies should be considered in treatment planning and appropriately compensated with IPR.

A proteção de dados pessoais e sensíveis da pessoa idosa, bem como a segurança digital são importantes para a efetividade dos direitos da personalidade. Por meio da pesquisa, serão evidenciados os resultados acerca da aplicabilidade de um ambiente digital seguro e como essa atividade auxilia na preservação de direitos intrínsecos desse grupo vulnerável, como o direito à vida privada, à intimidade e à imagem. O método dedutivo é utilizado, com pesquisa qualitativa e bibliográfica, com a análise da doutrina e legislação atual. Há o objetivo de construir uma visão jurídica diferenciada sobre a temática apresentada, tipificando e caracterizando quais são os maiores desafios enfrentados pela pessoa idosa em sua atividade na internet e como os seus dados são gerenciados e tratados, principalmente diante dos sites e aplicativos governamentais. É evidenciado, de igual forma, como a ingerência de tais dados interfere na efetividade e na proteção dos direitos da personalidade da pessoa idosa. Há pretensão de que o presente estudo possa ser instrumento para difundir cientificamente este tema, de maneira a acrescentar e fortalecer o conhecimento jurídico sobre seus impactos, além de identificar e promover possíveis soluções para a sua aplicabilidade ao efetivo cumprimento do princípio da dignidade da pessoa humana.

Brazilian men’s and women’s Artistic Gymnastics (AG) has been in the spotlight due to the results of some outstanding athletes in the most important international competitions over the last 20 years. However, the development of this sport in the national context does not reflect these achievements. This article performs a critical analysis of Women’s Artistic Gymnastics (WAG) in Brazil, reviewing the trajectory of the main gymnasts and their achievements in the World Championships and Olympic Games from 1966 to 2021, as well as relevant contextual facts during this historical period. Medals in major events have been won since 2001, with gradual and unstable growth, placing Brazil among the most prominent countries. Nevertheless, the data suggest that the achievements were based on concentrated investment in elite gymnasts, mainly in the Olympic team, with the support of already established clubs. The development is still centralized in a few clubs, without showing a sustainable increase in the number of clubs and gymnasts. In this scenario, there is a need for a long-term plan that can sustain the development of the national ecosystem (clubs, local competitions, provincial federations) across all age-group categories and in connection with the national teams..

Microorganisms are present everywhere and can influence a variety of processes. In agriculture and husbandry, the level of microbial activity can be crucial information, yet the methods for determining microbial activity are usually very long, complex, and costly. In this work, a novel and easy-to-use method, already in use for determining soil microbial activity, named Fertimetro was tested as a fast and cheap solution for measuring microbial activity in silages, in vitro rumen fluids, and manure and slurry. The method was adjusted for the specific conditions of the new testing environments. The results indicate that this method is adequate for measuring cellulolytic microbial activity in vitro rumen fluids, with a coefficient of repeatability (RT%) 92.2 at 24 h and 87.5 at 48 h, and also for cellulolytic microbial activity measures in manure RT% 39.0. While, due to the specific conditions in silages and slurry, this method is less adequate for measuring cellulolytic microbial activity in these environments. This work demonstrates that Fertimetro method can be used in different environments as an easy and cheaper alternative for measuring microbial activity, especially if the interest is only in quantifying the microbial activity and not in knowing the microbial species. 1. Fertimetro is an easy-to-use and not costly method to evaluate microbial activity in different environments. 2. This method is very adequate for measuring cellulolytic microbial activity in vitro rumen fluids and manure.

In this paper we tackle the feeling of a personality change users of more than one language reportedly experience when switching between their languages. We set to investigate if Croatian learners of English as a foreign language (EFL) experience a character trait change when switching between Croatian and English; which of the Big Five Inventory (BFI) personality dimensions change the most and which the least in Croatians using English, as well as whether there is a difference in this character trait change between different EFL proficiency groups. A total of 94 Croatian EFL speakers participated in the study. The sample was divided into a more proficient EFL speaker group (C1 level) and a less proficient EFL speaker group (B2 level). Each participant completed an EFL proficiency test and two sets of BFI questionnaires. One BFI set focused on the character traits the participants reported having while using Croatian; the second BFI set focused on the traits they reported having while using English. The results show that the participants experience a personality change when switching between their two languages and that different levels of language proficiency affect certain personality dimensions in EFL learners. In conclusion, we propose that the more proficient speakers may be bicultural and are experiencing cultural frame switching.

ABSTRACT: Udder health has a crucial role in sustainable milk production, and various reports have pointed out that changes in udder condition seem to affect milk mineral content. The somatic cell count (SCC) is the most recognized indicator for the determination of udder health status. Recently, a new parameter, the differential somatic cell count (DSCC), has been proposed for a more detailed evaluation of intramammary infection patterns. Specifically, the DSCC is the combined proportions of polymorphonuclear neutrophils and lymphocytes (PMN-LYM) on the total SCC, with macrophages (MAC) representing the remainder proportion. In this study, we evaluated the association between DSCC in combination with SCC on a detailed milk mineral profile in 1,013 Holstein-Friesian cows reared in 5 herds. An inductively coupled plasma–optical emission spectrometry was used to quantify 32 milk mineral elements. Two different linear mixed models were fitted to explore the associations between the milk mineral elements and first, the DSCC combined with SCC, and second, DSCC expressed as the PMN-LYM and MAC counts, obtained by multiplying the proportion of PMN-LYM and MAC by SCC. We observed a significant positive association between SCC and milk Na, S, and Fe levels. Differential somatic cell count showed an opposite behavior to the one displayed by SCC, with a negative association with Na and positive association with K milk concentrations. When considering DSCC as count, Na and K showed contrasting behavior when associated with PMN-LYM or MAC counts, with decreasing of Na content and increasing K when associated with increasing PMN-LYM counts, and increasing Na and decreasing K when associated with increasing MAC count. These findings confirmed that an increase in SCC is associated with altered milk Na and K amounts. Moreover, MAC count seemed to mirror SCC patterns, with the worsening of inflammation. Differently, PMN-LYM count exhibited patterns of associations with milk Na and K contents attributable more to LYM than PMN, given the nonpathological condition of the majority of the investigated population. An interesting association was observed for milk S content, which increased with increasing of inflammatory conditions (i.e., increased SCC and MAC count) probably attributable to its relationship with milk proteins, especially whey proteins. Moreover, milk Fe content showed positive associations with the PMN-LYM population, highlighting its role in immune regulation during inflammation. Further studies including individuals with clinical condition are needed to achieve a comprehensive view of milk mineral behavior during udder health impairment.

To evaluate the effectiveness of antiseptic mouthwashes in reducing SARS-CoV-2 load clinically and in vitro. A systematic electronic search (MEDLINE/Scopus/Cochrane) was conducted to identify prospective clinical and in vitro studies published between 2019 included and 16 June 2023 assessing the effectiveness of mouthwashes in reducing SARS-CoV-2 load in saliva or surrogates. Data were summarized in tables and a network meta-analysis was performed for clinical trials. Thirty-five studies (14 RCTs, 21 in vitro) fulfilled the inclusion criteria. The risk of bias was judged to be high for 2 clinical and 7 in vitro studies. The most commonly test product was chlorhexidine alone or in combination with other active ingredients, followed by povidone-iodine, hydrogen peroxide and cetylpyridinium chloride. Overall, the descriptive analysis revealed the effectiveness of the mouthwashes in decreasing the salivary viral load both clinically and in vitro. Network meta-analysis demonstrated a high degree of heterogeneity. Among these studies, only chlorhexidine 0.20% was associated to a significant Ct increase in the saliva 5 min after rinsing compared to non-active control (p = 0.027). Data from clinical and in vitro studies suggested the antiviral efficacy of commonly used mouthwashes. Large well-balanced trials are needed to identify the best rinsing protocols.

This study aimed to assess the environmental impact (cradle-to-farm gate LCA) of milk production of cereal-based dairy farms in northern Italy and to analyse which traits related to farm management (MAN_F), lactating cows’ ration composition (DIET_F), and animal responses (ANI_F) could be identified as main determinants to address specific mitigation strategies. Data originated from 28 dairy farms. The functional unit was 1 kg of fat- and protein-corrected milk (FPCM). Impact categories were global warming (GWP), acidification (AP) and eutrophication (EP) potentials and land occupation (LO). Impact values were analysed with a linear model to test separately the effects of MAN_F, DIET_F and ANI_F variables. One kg FPCM was associated with an average impact of 1.10 kg CO2-eq (GWP), 17.1 g SO2-eq (AP), 6.0 g PO4-eq (EP), and 1.3 m2/y (LO). Regarding MAN_F, GWP and LO linearly decreased with increasing values of stocking rate and feed self-sufficiency (FSS), whereas minimum EP was found for intermediate FSS values (50% DM). Regarding DIET_F, GWP linearly decreased at increasing levels of dietary inclusion of cereal silages, whereas AP and EP linearly decreased at decreasing dietary crude protein (CP) content values. Among ANI_F, GWP and EP were quadratically associated with milk yield (MY), evidencing the lowest values at intermediate MY (9100 ± 477 kg FPCM/cow per 305d-lactation). The GWP, AP and EP decreased with decreasing age at first calving. These results can contribute at drawing good practices for farmers and consultants to promote more environmentally sustainable dairy production while supporting farm functioning and farmers’ income.HIGHLIGHTS Farm and animal determinants of Life Cycle Assessment of cereal-based dairy farms were studied. Mitigation effects can be achieved by operating on farm organisation, cow’s diet, and animal responses. Good practices for farmers and consultants can be proposed for a better dairy production sustainability.

The milk Fourier-transform infra-red spectrometry (FTIRS) can be used to predict the enteric methane emissions (EME) at population level. In this study, the variability in FTIRS predicted EME traits due to the breed of cow, farm, the production level of individual cows within herds, and their interactions were evaluated. A dataset obtained from milk recordings, which covered four breeds (Holstein, Brown Swiss, Simmental and Alpine Grey), 6,430 herds, 115,819 cows, and 1,759,706 test-day milk/spectra records was used. The herds were stratified into 5 production levels considering their average daily milk energy production; individual cows within herd were similarly stratified considering their individual production levels. The EME traits were predicted directly from milk FTIR spectra or indirectly from six informative milk FA predicted from milk spectra. The statistical model included, separately for each trait and method, breed, herd intensiveness level, cow production level, and their interactions, year, month, parity, and lactation stage. The direct and indirect methods yielded similar results in predicting CH4 yield per kg of DMI and CH4 intensity per kg of fat-protein corrected milk. The indirect method was reliable in predicting daily EME production per cow, the indirect one did not. EME was affected by the breed x herd production intensiveness interaction, and to a lesser degree, by the breed x cow production level interaction. A better understanding of the complex interactions influencing EME in dairy herds was achieved. This would be useful for the genetic improvement, the environmental certification of farms, and for setting prices in milk payment schemes.HIGHLIGHTS Causes of variation of the FTIR predicted EME were studied at population level using data from 6,430 herds and 1,759,706 milk spectra records Reliable daily EME estimates were achieved from 6 six informative fatty acids predicted from milk spectra EME was influenced by the interaction breed × level of herd intensiveness and, to a lesser extent, by the individual production level

This work presents the development of a magnetic levitation system with a ferrite core, designed for electromagnetic energy harvesting from mechanical vibrations. The system consists of a fixed enamel-coated copper coil and five neodymium-iron-boron permanent magnets housed within a PVC spool. To enhance magnetic flux concentration, a manganese-zinc ferrite (Mn-Zn) ring was employed within the spool. Experimental tests were conducted at frequencies up to 20 Hz, demonstrating the device’s potential for harvesting energy from small vibrations, such as those generated by human biomechanical movements, achieving operating voltages up to 3 V. Additionally, the architecture is scalable for larger systems and allows for the integration of multiple transducers without magnetic field interference, independent of the frequency or excitation phase of each transducer.

As the global trend for healthy eating grows, firms are emphasising dairy functional foods (DFFs). This study looks into the relationship between consumer attitudes and readiness to consume DFFs, taking into account how a healthy lifestyle might both motivate and deter people from using them. Hungary was chosen because consumer behaviour towards functional foods is under-researched in comparison to Western Europe. Data were generated using a survey questionnaire from 313 respondents. The results of Partial Least Squares (PLS) analysis revealed that consumer attitudes had a considerable influence on the desire to consume DFFs. Furthermore, the motivators and barriers had a direct influence on willingness through the consumer attitudes that serve as mediators. These findings show the need for targeting consumer attitudes and lifestyle characteristics in order to increase the market acceptance of DFFs.

The aim of the present work was to evaluate retrospectively, after seven years of function, the efficacy of a conometric retention to stabilize complete prostheses (CPs) on four implants. Data from twenty-three patients with CPs supported by four implants, with at least seven years of follow up were retrieved. All the CPs were immediately fixed to the implants using a conometric retention. Outcome measures were prosthesis and implant success, biological and prosthetic complications, probing pocket depth changes, marginal bleeding, and plaque index changes. A total of 92 implants were evaluated. No fixture or abutment fractures were reported. No abutment unscrewing was reported. Four framework fractures occurred after three, four, six, and seven years of function. The overall success rate of the rehabilitation was 82.6%. Mucositis was observed in eight patients and 13 implants. No peri-implantitis was recorded. A 0.55 mm difference of PPD and 0.74 mm of MBL was recorded after seven years (p < 0.001). The present implant-supported conometric retention system proved to be effective in giving fixed retention to a CP supported by four implants. Biological complications were easily detected and treated. An adequate metal framework should be provided to the definitive restoration to avoid fractures in the long term.

Fluorescence spectroscopy is an extremely sensitive analytical technique that has been widely used to elucidate problems that require low detection limits. Although fluorescence spectroscopy is widespread in industry and academia, there is a lack of teaching materials demonstrating its basic principles, as well as the influence of instrumental parameters involved in the technique, which limit its correct use and the full potential of it. For this reason, this work aims to explore the principles of fluorescence spectroscopy and correlate them in function of different instrumental variables to carry out a fluorescence experiment. The spectrofluorometer parameters such as the excitation wavelength, excitation and emission slits, step, integration time, detection geometry as well as concentration of the samples were varied to illustrate how the best resulting spectra can be registered free of artifacts. To do so, two organic dyes with structured vibronic bands (pyrene) and another with unstructured bands (rhodamine 6G) were used. Another study carried out involved the use of 3D spectra, excitation/emission spectra, of cadmium telluride (CdTe) quantum dots. In this case, it was possible to show the excitation spectrum of nanomaterials, collecting emissions at different wavelengths, as well as analyzing the particle size distribution.

Il saggio pubblica e commenta la sentenza pronunciata dai giudici del Procurator di Venezia del 13 luglio 1366, documento che entrerà a far parte del Codice Diplomatico Poliano in preparazione a cura dell’Istituto Veneto di Scienze, Lettere ed Arti. La sentenza condanna i Procuratori di S. Marco, esecutori testamentari di Marco Bragadin (morto a Candia nel 1360) a pagare a sua moglie Fantina, primogenita di Marco Polo, 80 lire di grossi (800 ducati), considerato che Bragadin si era appropriato illegalmente del terzo dei beni mobili spettante alla moglie in quanto co-erede, assieme alle sorelle Bellela e Moreta, del Viaggiatore. Il notaio di curia, Fantino Rizzo, nel testo della sentenza inserì una copia fedelissima dell’inventario dei beni trovati nella casa di Marco Polo, redatto da Marco Bragadin poco dopo la morte del suocero, avvenuta l’8 o il 9 gennaio 1324: un testo giustamente famoso perché mostra molti punti di contatto con il Milione (o Devisament dou monde), il celebre libro scritto nel 1298 da Marco Polo e Rustichello da Pisa prigionieri nelle carceri genovesi.

Agroforestry systems may show low CO2 efflux, and CO2 efflux contributes to sustainability. This work aimed to evaluate the soil CO2 efflux in coffee plantations cultivated in agroforestry and full-sun systems during the winter in high-altitude tropical climate regions. The work was carried out at three family farms (RO, GI, and PA) in Minas Gerais, Brazil. Two treatments were established: coffee with and without trees, and 20 sampling spots for soil and gases. The air and soil temperatures in the agroforestry systems were lower than in the full-sun systems. The soil moisture content in agroforestry systems was higher than full-sun only on the GI. Except for the agroforestry systems in PA, all the other systems showed an increase in CO2 efflux with increasing soil moisture. This increase was more pronounced in agroforestry systems (RO), followed by full sun (RO). On the GI farm, this correlation was lower in the agroforestry system. Soil CO2 efflux was positively correlated with soil temperature and negatively correlated with total nitrogen, labile carbon and total organic carbon. Therefore, despite the microclimate stability promoted by the agroforestry systems in the winter, no decrease in the soil CO2 efflux was observed when compared to full sun systems.

Centipedegrass is a commonly grown, low input turfgrass used in landscapes throughout the state of Florida. This factsheet provide science based information on the culture and management of centipedegrass including mowing, irrigation, pest control, and cultural practices.