Search

The purpose of the study was to investigate the association of carriage of rs9939609 polymorphism in the FTO gene with features of androgen status in men.Material and methods. The observational one-stage examination of 139 male patients aged 22 to 69 years, admitted to a therapeutic hospital in a planned manner has been carried out in the Clinic of the Federal Research Center for Fundamental and Translational Medicine in the period 2020–2021. All fasting men in the morning (8:00–8:30) were carried out of an anthropometric examination to determine physical development, sampling of venous blood from the cubital vein to assess the hormonal parameters of androgen status, and taking a sample of the buccal epithelium for molecular genetic research. Based on the carriage of the rs9939609 (T>A) polymorphism of the FTO gene, the following groups (G) of patients were distinguished: G1 – wild-type homozygotes (WT/WT), G2 – heterozygotes (WT/A), G3 – homozygotes for the mutant allele (A/A).Results and discussion. The statistically significant differences were found in the levels of total testosterone between G1 and G3 (11.6 vs 14.5 nmol/L, p = 0.010), as well as free testosterone in G1 compared to G2 (0.233 vs 0.287 nmol/L, p = 0.012) and G3 (0.233 vs 0.321 nmol/L, p = 0.002) when analyzing the clinical-anthropometric and hormonal features of the androgen status of the examined male patients in the selected groups. Assessment of the association revealed statistically significant positive correlations between the carriage of the mutant allele and the levels of total (r = 0.247; p = 0.013) and free (r = 0.296; p = 0.003) testosterone, and negative - with the frequency of androgen deficiency according to the free testosterone criteria (r = –0.240; p = 0.016).Conclusions. It has been shown that the carriage of the A/A genotype of rs9939609 polymorphism of the FTO gene is associated with an increase in the levels of total and free testosterone, that indicates a protective effect against the development of androgen deficiency in men. The obtained results suggest that this effect is due to the epigenetic effect of the FTO gene protein product on the expression of other genes involved in the synthesis of androgens in the gonads, but also in the adrenal cortex since no association of the carriage of the rs9939609 polymorphism of the FTO gene with the DHEA-C level was found.

Introduction: It is well-known that there is an association between rs9939609 polymorphism of fat mass and obesity-associated (FTO) gene with obesity in people from different ethnic background. Objectives: This study aimed to assess the association of common polymorphism on adiposity indexes and type 2 diabetic mellitus (T2DM) and its association with adiponectin level in Iranian women. Patients and Methods: A sample population of 83 obese patients was investigated in a study with case-control design. The patients’ age, body mass index (BMI), systolic blood pressure (SBP), diastolic blood pressure (DBP), fasting blood sugar (FBS), insulin, insulin resistance, low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), glycated hemoglobin, triglycerides (TG) and adiponectin level were measured. The genotype of FTO rs9939609 was considered using specific primers via PCR and sequencing. Results: There was a significant difference between two groups (diabetic and non-diabetic) in terms of their age, FBS, HbA1c, LDL-C, SBP and DBP. The mean ± standard error (SE) of these parameters except for DBP and SBP were higher in diabetic group. The frequency of the TA genotype (48.27%) was higher in the diabetic group. The levels of FBS, HbA1c and insulin resistance index (HOMA-IR) were high in mutant group in comparison with wild group. There was no significant correlation between adiponectin level and anthropometric and metabolic parameters. However, in diabetic patients significant moderate positive correlation was found between HDL-C and adiponectin level (r = 0.473, P = 0.01). Conclusion: The association of rs9939609 FTO polymorphism was significant with FBS, HbA1c, TG, insulin, HOMA and adiponectin level in obese diabetic women who harbored the mutant A allele.

Abstract Tuberculosis and diabetes mellitus are the most important reasons in mortality all over the world. It’s clear that reviewing of relationship between Tuberculosis (TB) and diabetes mellitus (DM) is necessary; on the other hand it’s found that FTO gene has the positive relationship with metabolic issues. Therefore, it’s necessary pay attention to the above and dangerous sicknesses, especially in field of genetic sciences. Hence, our purpose is investigation of relationship between polymorphism rs9939609 polymorphism of FTO gene, TB and DM. With regards to the findings, it was concluded that there was considerable relationship between FTO gene rs9939609 polymorphism and DM and also it was notable development of TB among diabetic people. It can be stated that there is an indirect relationship between FTO gene rs9939609 polymorphism and TB sickness.