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Abstract The Radon and Hilbert transform and their applications to convectively coupled waves (CCWs) are reviewed. The Hilbert Transform is used to compute the wave envelope, whereas the Radon transform is used to estimate the phase and group velocities of CCWs. Together, they provide an objective method to understand CCW propagation. Results reveal phase speeds and group velocities for fast waves (mixed Rossby‐gravity, westward and eastward inertio‐gravity, and Kelvin) that are consistent with previous studies and with Matsuno's equatorial wave dispersion curves. However, slowly‐propagating tropical depression‐like systems and equatorial Rossby waves exhibit wave envelopes that propagate faster than the individual wave crests, which is not predicted by dry shallow water theory.

Abstract The moist processes of the Madden‐Julian Oscillation (MJO) in the Coupled Model Intercomparison Project Phase 6 models are assessed using moisture mode theory‐based diagnostics over the Indian Ocean (10°S–10°N, 75°E–100°E). Results show that no model can capture all the moisture mode properties relative to the reanalysis. Most models satisfy weak temperature gradient balance but have unrealistically fast MJO propagation and a lower moisture‐precipitation correlation. Models that satisfy the most moisture mode criteria reliably simulate a stronger MJO. The background moist static energy (MSE) and low‐level zonal winds are more realistic in the models that satisfy the most criteria. The MSE budget associated with the MJO is also well‐represented in the good models. Capturing the MJO's moisture mode properties over the Indian Ocean is associated with a more realistic representation of the MJO and thus can be employed to diagnose MJO performance.

To smelt beef tallow by using 1 000 kg as the mass of each sample of beef fat, and the fatty acid composition of the obtained butter including perirenal fat, abdominal fat and segmented fat was analyzed by gas chromatography (GC), and the volatile flavor components were detected by gas chromatography-olfactory mass spectrometry (GC-O-MS). The results showed that the saturated fatty acids (SFA) of the three kinds of beef tallow were mainly palmitic acid and stearic acid, and the unsaturated fatty acids (UFA) were mainly oleic acid. In addition, there was no significant difference (P>0.05) in palmitic acid content among three kinds of beef tallow. Although there were no significant differences in the stearic acid and oleic acid contents between perirenal tallow and belly tallow (P>0.05), there were significant differences in the stearic acid and oleic acid contents between partitioned tallow and waist oil and belly tallow (P

To provide an accurate and objective feedback on rehabilitation training movements and to improve the motivation of rehabilitation patients in rehabilitation training, a motion evaluation method capable of processing continuous human rehabilitation training movement data is proposed. First, a motion segmentation method based on the Gaussian mixture model (GMM) is developed to extract single motion repetition from continuous repetitive motion sequences of the same motion. Then, based on relevant a priori knowledge, a multi-feature fusion motion evaluation method combining significant motion feature dynamic time warping (DTW) distance evluation and Gaussian mixture model likelihood evaluation is proposed to perform motion evaluation in both the overall motion feature and local joint information of rehabilitation exercises. The results show that the motion segmentation method can segment the motion data of continuous repetitive motions well, and the correct rate of segmented motions on the dataset reaches more than 95%. The multi-feature fusion motion evaluation method effectively improves the differentiation of motion evaluation between healthy samples and rehabilitation patient samples, so that the motion scores of healthy samples are mainly distributed in the range of 0.93—0.94 on a scale of 0—1, while the motion scores of patient samples are mainly distributed in the range of 0.81—0.89.

Listeria monocytogenes (LM) is an important food-borne pathogen, and the risk of its ingestion is a serious public health issue. The better its environmental adaptation mechanisms and pathogenicity are understood, the better the risk it poses can be countered. The regulatory role of the small non-coding RNA (sRNA) rli106 in the environmental adaptation and pathogenicity of LM is still unclear and this study investigated that role through its biological function.

Nematodes of the Trichuris genus are commonly reported parasites that can cause trichuriasis in many animals, which leads to inflammation, intestinal bleeding and reductions of productivity in livestock. Knowledge of the prevalence of Trichuris infestation in the Tianshan ovine population and of the nematode species parasitising the population is not exhaustive, and this study aimed to expand the knowledge.

Objective To investigate the characteristics of gut microbiome and their associations with lymphocyte subsets and disease activity in patients with ankylosing spondylitis (AS). Methods This study was a retrospective analysis. The subjects of the study were AS patients who were hospitalized in the Second Hospital of Shanxi Medical University from December 2019 to June 2020, as well as gender- and age-matched healthy controls (HCs). The fecal samples were collected, and the V3-V4 variable regions of 16S rRNA gene of gut microbiome were sequenced for bioinformatics analysis. Peripheral venous blood was collected from AS patients to determine peripheral blood lymphocyte subsets and disease activity indicators. Spearman correlation test was used to analyze the correlations between the relative abundances of gut microbiota and peripheral blood lymphocyte subsets as well as disease activity in AS patients. Results A total of 62 AS patients (11 with low disease activity, 26 with high disease activity, and 25 with extremely high disease activity) and 62 healthy people who met the inclusion and exclusion criteria were enrolled. As for α-diversity, ACE and Chao1 indices were lower in AS than in HCs(P < 0.05). Bray curtis distance-based β-diversity analysis revealed significant difference in the microbial community between AS and HCs (P < 0.01). As for the composition of the gut microbiome, Firmicutes, Bacteroidetes, and Proteobacteria were the dominant phyla in the gut microbiota of both groups, but there were differences in the abundance of various bacteria at the phylum and genus levels. In Stamp analysis, fecal microbial communities in AS differed significantly from those in HCs, which were characterized by higher abundances of phylum Proteobacteria and Patescibacteria(all P < 0.05) and a lower abundance of phylum Firmicutes and Fusobacteriota (all P < 0.05). At the genus level, the abundances of Escherichia-Shigella, Klebsiella and Enterococcus were increased while those of Prevotella and Faecalibacterium were decreased in AS patients compared to HCs(all P < 0.05). Spearman correlation analysis showed that the relative abundances of Faecalibacterium, Ruminococcus and Klebsiella in AS patients were significantly positively correlated with disease activity or its related indicators(all P < 0.05). There were positive correlations between Agathobacter and T cell (r=0.302, P=0.017), CD4+T cell (r=0.310, P=0.014), B cell (r=0.292, P=0.021), Th2 cell (r=0.429, P < 0.001), Th17 cell (r=0.288, P=0.023), Streptococcus and B cell (r=0.270, P=0.034), Prevotella and Th1 cell (r=0.279, P=0.028), Th17 cell (r=0.262, P=0.040), CAG-352 and Th1 cell (r=0.283, P=0.030). There were negative correlations between Escherichia-Shigella and Th2 cell(r=-0.261, P=0.040), other Enterobacteriaceae and CD4+T cell (r=-0.255, P=0.046). Conclusions The diversity of gut microbiota is reduced in AS patients. The abundance of pathogenic bacteria in AS patients is increased, which is correlated with changes in peripheral blood lymphocyte subsets and disease activity. Dysbiosis may be involved in the occurrence and development of AS.

We examine the relationship between convectively coupled waves, the Madden–Julian Oscillation (MJO), and extreme precipitation over the western coast of South America during Coastal El Niño (COEN) events for the period spanning 1980–2023. Two types of COEN can be distinguished: (i) that occur in association with large-scale El Niño Southern Oscillation (ENSO) (e.g. 1982/83, 1997/98), and (ii) more ‘local’ COEN–when anomalous sea surface temperature take place over the far-eastern equatorial Pacific only (e.g. 2017, 2023). During both types of COEN events, increased rainfall along the western coast of South America is associated with intense Kelvin wave activity. In addition, westward inertio-gravity (WIG) waves, Rossby waves, and the MJO exhibit increased activity during local COEN events. During the recent extreme COEN 2017 and 2023, heavy rainfall occurred alongside significant WIGs, Kelvin, Rossby, and MJO events with unprecedented amplitudes propagating along western South America. Our results suggest that the probability of extreme precipitation under Coastal ENSO in western South America is strongly modulated by wave activity.

Key points 1. Simulations of atmospheric cloud-radiative effect (ACRE) in 54 CMIP6 models are evaluated against the satellite-based observational data. 2. The performance of ACRE simulations is sensitive to the model horizontal resolution used. 3. The finer resolution models generally produce a better performance of ACRE simulations, especially over the cold tongues where shallow stratocumulus clouds prevail.

Objectives: Clostridium perfringens (C. perfringens) can cause intestinal diseases in livestock and humans, which seriously threatens the healthy development of animal husbandry and human food safety. Here, the characteristics of antimicrobial resistance and molecular typing of ruminant-borne strains of C. perfringens in Xinjiang, China were explored and profiled. Methods: A total of 307 clinical feces collected from ruminants (cattle and sheep) with diarrheal symptoms were screened for C. perfringens. The recovered isolates were characterized in respect to their antimicrobial resistance pattern and molecular typing. Results: A total of 109 isolates of C. perfringens were isolated from 307 clinical feces of ruminants, most of which displayed the multidrug resistance (MDR) phenotype. Demonstration of the quinolone-resistance gene was the highest among the isolates (70.6%). The multiplex PCR typing based on toxin genes showed that type A and type D strains made up 82.6% (90/109) and 17.4% (19/109), among which, the isolates carrying β2 gene occupied 43.3% (39/90) of type A strains and 31.6% (6/19) of type D strains. These isolates were divided into 6 genotypes (I-VI) by enterobacterial repetitive intergenic consensus sequence-based PCR (ERIC-PCR) method. A total of 33 ST types (ST1-ST33) were identified by multilocus sequence typing (MLST) method. Conclusion: C. perfringens isolates with multidrug resistance (MDR) were frequent and circulating in ruminants. Among them, type A-Ⅰ-ST19 was the dominant genotype of C. perfringens, displaying obvious genetic diversity. This study provided important epidemiological data for the risk assessment of food safety associated with ruminant-borne C. perfringens in Xinjiang, China.

Carbon nanotubes(CNTs), as an important discovery in the research of nanomaterials, have become a research hotspot in the field of carbon materials since their birth. With its unique porous structure, metal-organic frameworks (MOFs) has been developed into one of the frontiers of research in recent years. With the continuous development of materials science in recent years, the research on composite technology of materials with different functional characteristics has become one of the main methods to solve key problems in the field of materials applications.CNTs and MOFs are two very important types of nanomaterials in the current material field. Combining the high electrical conductivity of CNTs with the high specific surface area and rich pore distribution characteristics of MOFs through composite technology is an inevitable trend for future research and application in the field of materials. In this paper, the main composite forms and preparation methods of MOFs and CNTs in recent years were reviewed, and the latest research progress of composites in the fields of supercapacitors, lithium battery electrodes, catalysis, adsorption, etc. was summarized. The synergistic improvement of the performance of the two materials was discussed and analyzed, and it was pointed out that the composite of CNTs and MOFs materials and the growth and distribution of CNTs have a high degree of randomness, and further control of them is the focus of future technical research.

As one of important candidates for advanced aerocraft engine hot components, SiC/SiC composites would undergo oxidative and corrosive damage when subjected to high temperature combustion environment. Oxidation behavior of SiC/SiC composites at 1100-1300 ℃ in air was investigated in the present work, to clarify the oxidative and corrosive behavior of the composites. The oxidation kinetics curves were obtained. The morphology and composition evolution of the oxidized materials were analyzed by SEM, XPS and XRD. The results show that the oxidation kinetics of SiC/SiC composites at 1100-1300 ℃ follow the parabolic law and the oxides on the surface mainly consist of silica. Slight oxidation occurs at 1100 ℃. The oxidation rate increases with increasing temperature. The oxidation process is mainly concentrated in the BN interface phase and the pores of the matrix. The bending strength of the composite decreases with the oxidation extent.

Diabetes mellitus is a fast-growing disease with a major influence on people’s quality of life. Oral hypoglycemic drugs and insulin are currently the main effective drugs in the treatment of diabetes, but chronic consumption of these drugs has certain side effects. Polysaccharides, saponins, flavonoids, and phenolics are the primary secondary metabolites isolated from the rhizomes of Polygonatum sibiricum Redouté [Asparagaceae], Polygonatum kingianum Collett & Hemsl [Asparagaceae], or Polygonatum cyrtonema Hua [Asparagaceae], which have attracted much more attention owing to their unique therapeutic role in the treatment and prevention of diabetes. However, the research on the mechanism of these three Polygonatum spp. in diabetes has not been reviewed. This review provides a summary of the research progress of three Polygonatum spp. on diabetes and its complications, reveals the potential antidiabetic mechanism of three Polygonatum spp., and discusses the effect of different processed products of three Polygonatum spp. in treating diabetes, for the sake of a thorough understanding of its effects on the prevention and treatment of diabetes and diabetes complications.

Background and Aim: As a tick-borne zoonotic pathogen, Ehrlichia canis has already posed a threat to public health and safety. This study aimed to clarify the prevalence and molecular characteristics of E. canis in pet dogs in Xinjiang, China. Materials and Methods: A total of 297 blood samples of pet dogs and 709 skin ticks (Rhipicephalus sanguineus sensu lato) were subjected to molecular detection using PCR for E. canis 16S rRNA gene, and then, positive samples were amplified, sequenced, and phylogenetically analyzed for E. canis gp36 gene. Results: The PCR detection showed that the positive rate of PCR was 12.12% (36/297) in blood samples and 15.23% (108/709) in tick samples, respectively. Based on the phylogenetic analysis of E. canis gp36 protein, these E. canis strains in different geographical regions of the world can be divided into Genogroup I and Genogroup II. Among them, the Xinjiang epidemic strain XJ-6 and 533, 36, 1055, Kasur1, and Jake strains were clustered into subgroup 1.1 of Genogroup I, while the XJ-2, XJ-21, and XJ-35 strains and the TWN1, TWN4, CM180, and CM196 strains were closely related and belonged to subgroup 2.2 of Genogroup II, displaying high genetic diversity. Conclusion: This is the first study focusing on the molecular epidemiology of E. canis infection in pet dogs, which revealed that E. canis infection had been occurred in Xinjiang, China. More importantly, this study confirmed that the substantial variability in immunoreactive protein gp36 from E. canis strains circulating in pet dogs.

Objective To analyze the clinical features of autonomic nerve dysfunction and sympathetic skin response (SSR) in patients with gout. Methods A total of 25 patients with gout diagnosed from March 2017 to January 2018 were enrolled, including 10 patients with tophus and 8 patients with autonomic nerve dysfunction(symptomatic). EMG was used to detect SSR. Results The main symptoms of autonomic nervous dysfunction in the gout group were abnormal sweat glands. Compared with the normal control group, the lower extremity latency period of patients in the gout group was prolonged (Z=⁃2.136, P =0.033) and the amplitude was reduced (Z =⁃2.959, P =0.003). Lower extremity amplitude of the symptomatic group was lower than that of the normal control group (Z =⁃3.426, P = 0.001) and the asymptomatic group (Z = ⁃3.308, P=0.001). The lower extremity amplitude of patients in tophout group was lower than that in the control group(Z =⁃3.176, P=0.001) and the non⁃tophout group(Z =⁃2.301, P= 0.021), while the lower extremity latency period of patients without tophout was prolonged(Z=⁃2.518, P= 0.120)and the amplitude was reduced(Z=⁃2.327, P=0.020),which were statistically significant different from the control group. Conclusions Abnormal autonomic nerve function exists in gout patients, mainly in lower limb injury, especially in patients with tophus or symptoms of autonomic nerve dysfunction. SSR can objectively evaluate the autonomic nerve function state.

Attenuation of noise-induced hair cell loss and noise-induced hearing loss (NIHL) by treatment with FK506 (tacrolimus), a calcineurin (CaN/PP2B) inhibitor used clinically as an immunosuppressant, has been previously reported, but the downstream mechanisms of FK506-attenuated NIHL remain unknown. Here we showed that CaN immunolabeling in outer hair cells (OHCs) and nuclear factor of activated T-cells isoform c4 (NFATc4/NFAT3) in OHC nuclei are significantly increased after moderate noise exposure in adult CBA/J mice. Consequently, treatment with FK506 significantly reduces moderate-noise-induced loss of OHCs and NIHL. Furthermore, induction of reactive oxygen species (ROS) by moderate noise was significantly diminished by treatment with FK506. In agreement with our previous finding that autophagy marker microtubule-associated protein light chain 3B (LC3B) does not change in OHCs under conditions of moderate-noise-induced permanent threshold shifts, treatment with FK506 increases LC3B immunolabeling in OHCs after exposure to moderate noise. Additionally, prevention of NIHL by treatment with FK506 was partially abolished by pretreatment with LC3B small interfering RNA. Taken together, these results indicate that attenuation of moderate-noise-induced OHC loss and hearing loss by FK506 treatment occurs not only via inhibition of CaN activity but also through inhibition of ROS and activation of autophagy.

Fascioliasis is an important zoonotic helminthic disease caused by Fasciola hepatica and poses a serious threat to global public health. To evade the immune response of its host (humans or animals), F. hepatica secretes various antioxidant enzymes such as glutathione transferase (GST) to facilitate its invasion, migration and parasitism in vivo. To investigate the biological functions of a novel omega-class GST (GSTO), the molecular features of GSTO2 of F. hepatica were analyzed by online software, and the biochemical properties in vitro of recombinant GSTO2 (rGSTO2) were dissected. Then, the regulatory roles of rGSTO2 protein in murine macrophages in vitro were further explored. The results revealed that the GSTO2 gene encodes 254 amino acids, which harbor the characteristic N-terminal domain (βαβαββα) and C-terminal domain (α-helical) of the cytoplasmic GST superfamily. GSTO2 was mainly expressed in F. hepatica vitelline follicles, intestinal tract, excretory pores and vitelline cells, with thioltransferase and dehydroascorbate reductase activities. Moreover, rGSTO2 protein could be taken up by murine macrophages and significantly inhibit the viability of macrophages. In addition, rGSTO2 protein could significantly promote apoptosis and modulate the expression of cytokines in macrophages. These findings suggested that F. hepatica GSTO2 plays an important role in modulating the physiological functions of macrophages, whereby this protein might be involved in immunomodulatory and anti-inflammatory roles during infection. This study provided new insights into the immune-evasion mechanism of F. hepatica and may contribute to the development of a potential anti-inflammatory agent.

Objective To establish an analytical method of the endosulfan concentrations （α-endosulfan and β-endosulfan） in biological samples by GC-MS/MS. To observe the distribution of endosulfan in aquatic animals and provide experimental evidence for forensic identification of relevant cases. Methods Acetonitrile was added to the blood and muscle samples for precipitating the protein. The endosulfan concentrations were determined by GC-MS/MS in multiple reaction monitoring mode. Qualitative analysis was performed according to the retention time and ion rate, and quantitative analysis was performed by external standard working curve method. Results In blood samples, the calibration curves of α-endosulfan and β-endosulfan ranging from 0.062 5 to 10 μg/mL had good linear relationship, the correlation coefficients （r） of which were >0.99. The limits of detection （LOD） were 1 ng/mL and 2 ng/mL and the limits of quantification （LOQ） were 4 ng/mL and 8 ng/mL, respectively. In muscle samples, the calibration curves of α-endosulfan and β-endosulfan ranging from 0.062 5 to 10 μg/g, the r of which were >0.98. The LOD were 1 ng/g and 4 ng/g and the LOQ were 4 ng/g and 16 ng/g, respectively. The accuracy of α-endosulfan and β-endosulfan was 90.76%-108.91% both in blood and muscle samples, the interday and intraday precision were 2.35%-8.71% and 5.44%-10.29%, respectively. In poisoning cases, endosulfan were detected in all parts of fish and crab and the content difference was statistically significant. Conclusion The endosulfan detection method based on GC-MS/MS established in the present study is rapid, sensitive and accurate, which can be applied to the endosulfan detection in traces biological samples. The distribution of endosulfan in fish and crab was different, which can provide evidence to the sample collection and analysis for toxicological analysis in relevant forensic identification.

Twin-roll strip casters for near-net-shape manufacture of steels have received increased attention in the steel industry. Although negative segregation of phosphorus occurred in twin-roll strip casting (TRSC) steels in our prior work, its mechanism is still unclear. In this work, V-shaped molds were designed and used to simulate a meta-rapid solidification process without roll separating force during twin roll casting of carbon steels. Experimental results show that no obvious phosphorus segregation exist in the V-shaped mold casting (VMC) steels. By comparing TRSC and the VMC, it is proposed that the negative phosphorus segregation during TRSC results from phosphorus redistribution driven by recirculating and vortex flow in the molten pool. Meanwhile, solute atoms near the advancing interface are overtaken and incorporated into the solid because of the high solidification speed. The high rolling force could promote the negative segregation of alloying elements in TRSC.

Abstract Background Acute autonomic neuropathy (AAN) is rare disorder with anecdotal report, especially for childhood onset patients. Misdiagnosis or delays in treatment can always be found in clinical practice. We conducted this study to give a description of the manifestations and treatment of AAN in children and therefore help clinicians to make the accurate diagnosis early so that the prognosis of the patients can be improved. Methods A systematic record from 3 clinical centers was used to identify 11 subject, 3 males and 8 females, with clinical diagnosed AAN. Result The age ranged from 2 years and 4 months to 14 years and 6 months (mean, 9 ± 3.6 years old) and the course from onset to diagnosis ranged from 7 days to 8 months. All children shared prominent initial symptoms, 7 with frequent vomiting and 4 with motor dysfunctions. The condition of 9 patients improved after treatment of IVIg and intravenous glucocorticoid. Conclusion The clinical manifestations of AAN are diverse, generalized, and non-specific. Gastrointestinal disorders were the most common initial symptoms. Symptoms of gastrointestinal system and abnormal secretion of glands were severe and more common than other symptoms. The mechanism of AAN remains unknown. Although IVIg and intravenous glucocorticoid can be used in clinical practice, there is still no treatment recommendation and further study is needed.

For violent vibration of the sink roll device of a hot-dip galvanizing line, an incomplete liquid-solid coupling method based on the wet modal was studied, the vibration modal of the device was numerically simulated, the natural frequencies and vibration modes were discussed, and the vibration test was performed. The partial mode of the sink roll changed the assembly relationship in bearings, and the clipping appeared in the time domain waveform of measured signals, indicating that crashing and friction existed in the sliding bearings. The frequency of the peak in the amplitude spectrum was 45.05 Hz, close to the fourth order frequency and mode, which showed that the fourth order natural vibration of the device was dominant. The double-frequency vibration was caused by nonlinear vibration of liquid-solid coupling. The main cause of sidebands near the peak in the amplitude spectrum came from the continuous action of the zinc liquid flow field. The numerical analysis and experimented research have significant correlation, and the incomplete solid-liquid coupling method can be effectively applied to engineering practice.

Graphene oxide and carbon nanotubes supported Pt nanoparticles were successfully synthesized by wet chemical reduction method. The structures and methanol oxidation performance of the two catalysts were characterized by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray diffraction (XRD), Raman spectroscopy (Raman) and electrochemical workstation. The results show that graphene oxide supported Pt nanoparticles exhibits higher electrochemical active surface area, methanol oxidation activity and stability compared with carbon nanotubes. It is proposed that the well dispersed Pt on the graphene oxide plays an important role for the excellent performancefor methanol oxidation.

In this study, N, N’-carbonic bis(piperonylic acid) dihydrazide (BPACH) was synthesized to broaden the category of piperonylic acid derivative and evaluate its influences on the thermal properties of poly(L-lactic acid) (PLLA). The geometry optimization of BPACH showed that the highest occupied molecular orbital mainly focused on the formed amide group and carbonic dihydrazide, whereas the lowest unoccupied molecular orbital mainly focused on the piperonylic acid, and the orbital energy gap was 0.10418 eV. The differences in melt-crystallization processes of the neat PLLA and PLLA/BPACH samples indicated that the BPACH could provide the effective nucleation site to accelerate the crystallization of PLLA, but the crystallization accelerating effect was still further improved compared to some reported nucleating agents. The melting behaviors of PLLA/BPACH samples after crystallization depended on the crystallization temperatures and heating rates; additionally, the melting processes could also effectively reflect the previous crystallization behaviors.

Ferroptosis is a process driven by accumulated iron-dependent lipid ROS that leads to cell death, which is a distinct regulated cell death comparing to other cell death. The lethal metabolic imbalance resulted from GSH depletion or inactivation of glutathione peroxidase 4 is the executor of ferroptosis within the cancer cell. Small molecules-induced ferroptosis has a strong inhibition of tumor growth and enhances the sensitivity of chemotherapeutic drugs, especially in the condition of drug resistance. These evidences have highlighted the importance of ferroptosis in cancer therapeutics, but the roles of ferroptosis in tumorigenesis and development remain unclear. This article provides an overview of the mechanisms of ferroptosis, highlights the role of ferroptosis in cancer and discusses strategies for therapeutic modulation.

Acotiamide hydrochloride trihydrate, which is the first gastrointestinal prokinetic agent, has been proven effective in treating functional malnutrition. There is little method in analyzing this new medicine. The only reported method is using High Performance Liquid Chromatography (HPLC), with methanol-monopotassium phosphate-sodium octanesulfonate as the mobile phase. However, this method is time wasting and unstable because of the using of an ion paring agent in the mobile phase. In this paper, a new HPLC method is established to quantitatively analyze industrial acotiamide hydrochloride trihydrate based on its amphoteric structure. The separation is accomplished in a C18 (4.6 mm×150 mm, 5 μm) column with methanol and 20 mmol/L ammonium acetate aqueous solution (pH 6.8) (45:55, V/V) as the mobile phase. The detection wavelength is set at UV 280 nm. The retention time of acotiamide hydrochloride trihydrate is 8 min with good peak shape, and the total analysis time is 23 min. Under the optimized chromatographic condition, the calibration curve of acotiamide hydrochloride trihydrate is linear in the range of 0.0006 and 1.0 mg/mL, detection limit is 0.0002 mg/mL, and both the intra-and inter-day relative standard deviations (RSDs) are lower than 1%. This method contains no ion paring agent, benefiting the rapid balance of chromatographic condition and improving the analysis repeatability and maneuverability. The method can be performed for rapid detection of acotiamide hydrochloride trihydrate and used in the quality control of raw medicine.

Acotiamide hydrochloride trihydrate, which is the first gastrointestinal prokinetic agent, has been proven effective in treating functional malnutrition. There is little method in analyzing this new medicine. The only reported method is using High Performance Liquid Chromatography (HPLC), with methanol-monopotassium phosphate-sodium octanesulfonate as the mobile phase. However, this method is time wasting and unstable because of the using of an ion paring agent in the mobile phase. In this paper, a new HPLC method is established to quantitatively analyze industrial acotiamide hydrochloride trihydrate based on its amphoteric structure. The separation is accomplished in a C18 (4.6 mm×150 mm, 5 μm) column with methanol and 20 mmol/L ammonium acetate aqueous solution (pH 6.8) (45:55, V/V) as the mobile phase. The detection wavelength is set at UV 280 nm. The retention time of acotiamide hydrochloride trihydrate is 8 min with good peak shape, and the total analysis time is 23 min. Under the optimized chromatographic condition, the calibration curve of acotiamide hydrochloride trihydrate is linear in the range of 0.0006 and 1.0 mg/mL, detection limit is 0.0002 mg/mL, and both the intra-and inter-day relative standard deviations (RSDs) are lower than 1%. This method contains no ion paring agent, benefiting the rapid balance of chromatographic condition and improving the analysis repeatability and maneuverability. The method can be performed for rapid detection of acotiamide hydrochloride trihydrate and used in the quality control of raw medicine.

A novel VP-16 derivative, 4β-[N -(4‴-acetyloxyl-phenyl-1‴-carbonyl)-4″-aminoanilino]-4′-O-demethyl-4-desoxypodophyllotoxin (GL3), displayed a wide range of cytotoxicity in a panel of human tumor cell lines, with half-maximal inhibitory concentration (IC50) values ranging from 0.82 to 4.88 µM, much less than that of VP-16 (4.18–39.43 µM). Importantly, GL3 induces more significant apoptosis and cell cycle arrest than VP-16. The molecular and cellular machinery studies showed that GL3 functions as a topoisomerase II (Top 2) poison through direct binding to the enzyme, and the advanced cell-killing activities of GL3 were ascribed to its potent effects on trapping Top 2-DNA cleavage complex, Moreover, GL3-triggered DNA double-strand breaks and apoptotic cell death were in a Top 2-dependent manner, because the catalytic inhibitor aclarubicin attenuated these biologic consequences caused by Top 2 poisoning in GL3-treated cells. Taken together, among a series of 4β-anilino-4′-O-demethyl-4-desoxypodophyllotoxin analog, GL3 stood out by its improved anticancer activity and well-defined Top 2 poisoning mechanisms, which merited the potential value of GL3 as an anticancer lead compound/drug candidate deserving further development.

The paper analyzed problems of original design scheme of high voltage capacitor cabinet of Baimahe air shaft substation in Nantun Coal Mine, and gave its improvement design: selecting vacuum circuit breaker for capacitive load; adding discharge coil and connecting the coil with a capacitor in parallel directly to form a complete discharge circuit; adding grounding switch which has both mechanical latch and electrical interlock for easy operation and high security. The practical application shows that the improved high-voltage capacitor cabinet has more functions and high security and reliability.

Speed of heavy vehicles is greatly reduced on upgrade sections due to their insufficient climbing capability, thus having great impact on traffic safety. Setting climbing lanes can solve this problem effectively. Considering China’s traffic composition and operational status, this paper proposed the traffic conflict technique-based setting method of freeway climbing lanes in order to set foundations for climbing lanes with the goal of promoting traffic safety. Based on large freeway upgrade section data and actual traffic characteristics, the paper built simulation models with VISSIM. 220 schemes were simulated by setting different road and traffic conditions of models. The paper then used Surrogate Safety Assessment Model (SSAM) to calculate traffic conflict, analyzed the influence of slope, slope length and mixed rate of heavy vehicles on traffic safety on upgrade sections. Finally, taking the reduction extent of the collision rate after setting climbing lanes as an indicator, this paper used K-means to classify the effectiveness level of the climbing lanes. The paper found the setting position and basis of the climbing lanes. The findings of this paper can provide support to effective decision making with regards to constructing future climbing lanes.

The human hepatocellular carcinoma (HCC) represents biologically aggressive and chemo-resistant cancers. Owing to the low affinity with the apoptotic factor Mcl-1, the BH3 mimetic drug ABT-737 failed to exert potent cancer-killing activities in variety of cancer models including HCC. The current study demonstrated that combining ABT-737 and Celastrol synergistically suppressed HCC cell proliferation, and induced apoptosis which was accompanied with the activation of caspase cascade and release of cytochrome c from mitochondria. Further study revealed that the enhanced Noxa caused by Celastrol was the key factor for the synergy, since small interfering RNA-mediated knockdown of Noxa expression in HCC cells resulted in decreased apoptosis and attenuated anti-proliferative effects of the combination. In addition, our study unraveled that, upon Celastrol exposure, the activation of endoplasmic reticulum (ER) stress, specifically, the eIF2α-ATF4 pathway played indispensable roles in the activation of Noxa, which was validated by the observation that depletion of ATF4 significantly abrogated the Noxa elevation by Celastrol. Our findings highlight a novel signaling pathway through which Celastrol increase Noxa expression, and suggest the potential use of ATF4-mediated regulation of Noxa as a promising strategy to improve the anti-cancer activities of ABT-737.