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In a two-year Hungarian study, the temporal progress of brown rot incidence and various injury types were studied in organic fruit orchards and the relationship between brown rot and injury types was determined. Results showed that brown rot reached an almost 20% incidence level in both years. Total injury incidence reached up to 5.3 and 19.8% in the two years. In all cases, insect injury incidence was the highest among injury types in most assessment dates. Incidence levels of other injury types (bird injury, mechanical injury and other injury) began to increase, but none of those reached levels >4%. Pearson’s correlation coefficients showed that brown rot incidence correlated significantly with the incidence of insect injury. In addition, brown rot incidence and the incidence levels of bird injuries was also significantly correlated. High injury and brown rot incidence levels suggest further improvements on organic fruit protection.

In a two-year Hungarian study, spore dispersal diurnal periodicity and viability of Monilinia spp. and their relation to weather components were determined in an organic apple orchard. Conidia of Monilinia spp. were first trapped in late May in both years. Low number of conidia were trapped until end-June. Thereafter, number of conidia continuously increased until harvest. Conidia in a 24-h period showed diurnal periodicity pattern, with th highest concentration in the afternoon hours. Spore viability with FDA staining showed that viability of conidia ranged from 45 to 70% with showing lower viability in the dry than in the wet days in both years. Temperature and relative humidity correlated positively with mean hourly conidia numbers in both years. Mean hourly rainfall was negatively but poorly correlated with conidiacatches in both years. Results were compared and discussed with previous observations.

In this study, the objective was to report a two-year investigation on micro area based spatial distribution of apple scab in an organic apple orchard. Results showed that number of symptomatic plant part ranged between 587 and 623 on leaf and between 46 and 78 on fruit for an individual tree. Number of asymptomatic plant part ranged between 1034 and 1321 on leaf and between 119 and 193 on fruit. Disease incidence ranged between 35 and 40% on leaf and between 27 and 33% on fruit. Disease aggregation index ranged between 0.115 and 0.298 on leaf and between 0.117 and 0.221 on fruit. Three of the four trees showed significant within canopy aggregation of disease for leaf apple scab symptoms in both years. For fruit apple scab, two of the four trees showed significant random patterns in both years.

The aim of this study was to evaluate the effectiveness of reduced spray programmes against cherry leaf spot in integrated and organic sour cherry orchards. Altogether four spray programmes were performed i) standard integrated: sprays followed by forecasting systems during the season, ii) reduced integrated: sprays followed by forecasting systems but only 75% of the spray numbers used during the season-long spray programme, iii) standard oragnic: sprays applied every 7–14 days during the season and iv) reduced organic: 60 % of the spray numbers used during the season-long spray programme. Our study showed that cherry leaf spot did not increase above 3.1, 7.4, and 8.9% in 2008, 2009, and 2010, respectively, in the integrated orchards. However, leaf spot incidence was above 20% in all years in the organic field. Reduced spray programme did not increase significantly cherry leaf spot incidence in the integrated field in either years. However, leaf spot incidence increased significanly (above 20%) in the reduced spray programme for the organic orchard.

In this study, possibilities of environmentally-benign plant protection against blossom and twig blight were summarized for organic stone fruit orchards. Symtomps of Monilinia laxa (Aderh. & Ruhl.) Honey) were described and then cultivar susceptibility to blossom and twig blight was discussed. Several sustainable plant protection methods were selected and discussed in details such as mechanical, agrotechnical, biological, and other non-chemical control possibilities (stone powders, plant extracts and restricted chemical materials).

The aim of this study was first to test the in vitro effeicacy of some fungicides against brown rot of sour cherry, and secondly to evaulate the effectiveness of reduced spray programmes against brown rot in integrated and organic sour cherry orchards. In vitro efficacy of 7 fungicides (Champion 50 WP, Kocide 2000, Nordox 75 WG, Olajos rézkén, Kumulus S, Rézkén, Rézoxiklorid) and another 6 fungicides (Score 25 EC, Efuzin 500 SC, Systane, Folicur Solo, Zato Plusz, Rovral) approved in organic and integrated production systems, respectively, were tested against brown rot of sour cherry. Altogether four spray programmes were performed i) standard integrated: sprays followed by forecasting systems during the season, ii) reduced integrated: sprays followed by forecasting systems but only 75% of the spray numbers used during the season-long spray programme, iii) standard oragnic: sprays applied every 7–14 days during the season and iv) reduced organic: 60% of the spray numbers used during the season-long spray programme. In vitro results showed that fungicides (with active ingredients of copper and sulphur) applied in organic production showed relatively high percent growth capacity of Monilinia fungus. Rézkén showed the highest and Kumilus S the lowest efficacy against brown rot. Fungicides applied in integrated production showed relatively low percent growth capacity of Monilinia fungus. Score 25 EC showed the highest and Rovral the lowest efficacy against brown rot. Field study showed that reduced spray programmes did not increase significantly brown rot incidence in the integrated field. However, brown rot incidence increased significanly (above 30%) in the reduced spray programme for the organic orchard.

In this review, some aspects of disease management of cherry leaf spot (Blumeriella jaapii) are summarised with special reference to pesticide use. In the first part of the review, we show the non-chemical control approach (e. g. removal of fallen leaves, planting resistant cultivar) against leaf spot. In the second part of the review, the effect of pesticides including fertilizers (urea) and fungicides on cherry leaf spot are discussed. Special attention are given to the fungicides of copper, dodine, captafol, captan, benomil, chlorothalonil, sterol demethylation inhibitors (e.g. fenarimol, fenbuconazole, myclobutanil, tebuconazole), and strobilurins about their effectiveness against cherry leaf spot. In the final part of the review, possibilities of cherry leaf spot control are discussed in integrated and organic cherry orchards.

Still a large amount of pesticides and spary applications are used in environmentally friendly fruit production systems; therefore, the aim of our study was first to test the in vitro effeicacy of some fungicides against a key apple disease (apple scab), and secondly to evaulate the effectiveness of reduced spray programmes against apple scab, powdery mildew and coding moth in integrated and organic apple orchards. In vitro efficacy of 7 fungicides (Champion 50 WP, Kocide 2000, Nordox 75 WG, Olajos rézkén, Kumulus S, Rézkén, Rézoxiklorid) and another 6 fungicides (Score 25 EC, Efuzin 500 SC, Systane, Folicur Solo, Zato Plusz, Rovral) approved in organic and integrated production systems, respectively, were tested against apple scab. Altogether four spray programmes were performed i) standard integrated: sprays followed by forecasting systems during the season, ii) reduced integrated: sprays followed by forecasting systems but only 75% of the spray numbers used during the season-long spray programme, iii) standard oragnic: sprays applied every 7–14 days during the season and iv) reduced organic: 60% of the spray numbers used during the season-long spray programme. In vitro results showed that fungicides (with active ingredients of copper and sulphur) applied in organic production showed relatively high percent growth capacity of the apple scab fungus. Rézkén showed the highest and Kumilus S the lowest efficacy against apple scab. Fungicides applied in integrated production showed relatively low percent growth capacity of the apple scab fungus. Score 25 EC showed the highest and Rovral the lowest efficacy against apple scab. Field study showed that reduced spray programmes did not increase significantly scab incidence in the integrated field. However, scab incidence increased significanly (above 30%) in the reduced spray programme for the organic orchard.Mildew incidence was low (below 5%) in both integrated and organic spray programmes. Mildew incidence on both shoots and fruits increased significanly in the reduced spray programme for the organic orchard. Incidence of codling moth damage was affected the most by standard vs. reduced spray programmes. Though incidence remained below 10% in the integrated plots, the incidence was significantly higher in the reduced spray programme compared to the standard programme. Similar results were obtained for organic spary programmes, but the incidence was 10 times higher and the differences among the two programmes were larger.,

The current treatment of neuropathic pain (NP) is unsatisfactory; therefore, effective novel agents or combination-based analgesic therapies are needed. Herein, oral tolperisone, pregabalin, and duloxetine were tested for their antinociceptive effect against rat partial sciatic nerve ligation (pSNL)-induced tactile allodynia described by a decrease in the paw withdrawal threshold (PWT) measured by a dynamic plantar aesthesiometer. On day 7 after the operation, PWTs were assessed at 60, 120, and 180 min post-treatment. Chronic treatment was continued for 2 weeks, and again, PWTs were measured on day 14 and 21. None of the test compounds produced an acute antiallodynic effect. In contrast, after chronic treatment, tolperisone and pregabalin alleviated allodynia. In other experiments, on day 14, the acute antiallodynic effect of the tolperisone/pregabalin or duloxetine combination was measured. As a novel finding, a single dose of the tolperisone/pregabalin combination could remarkably alleviate allodynia acutely. It also restored the neuropathy-induced elevated CSF glutamate content. Furthermore, the combination is devoid of adverse effects related to motor and gastrointestinal transit functions. Tolperisone and pregabalin target voltage-gated sodium and calcium channels, respectively. The dual blockade effect of the combination might explain its advantageous acute analgesic effect in the present work.